Nocardioides sp. CF204

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3302 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T5W9M8|A0A2T5W9M8_9ACTN Single-strand DNA-binding protein OS=Nocardioides sp. CF204 OX=2135677 GN=C8J51_2070 PE=4 SV=1
MM1 pKa = 7.97RR2 pKa = 11.84PPFLRR7 pKa = 11.84TTLAGMLVAATLGLAPAAFADD28 pKa = 3.71QTSRR32 pKa = 11.84DD33 pKa = 3.45GDD35 pKa = 3.76TAAVGRR41 pKa = 11.84NITYY45 pKa = 10.22GPGFRR50 pKa = 11.84ACTTRR55 pKa = 11.84GAQVSGAITVTYY67 pKa = 10.78NGGTHH72 pKa = 6.17LTPGEE77 pKa = 3.98ALVVTMSPPAGVTATAGATSVPATWNSNGQSFVIPLSTTVTTAAASGSVGFKK129 pKa = 10.68VVGASSGYY137 pKa = 10.57DD138 pKa = 3.22VTAGGSQYY146 pKa = 11.47DD147 pKa = 4.08VIVDD151 pKa = 4.28CPANTAPTGVADD163 pKa = 5.17SYY165 pKa = 11.67ATDD168 pKa = 3.42EE169 pKa = 4.32DD170 pKa = 4.29TAKK173 pKa = 10.6VVAAPGVLGNDD184 pKa = 3.6TDD186 pKa = 4.09PQGDD190 pKa = 3.94ALTAEE195 pKa = 4.52LVGGPVHH202 pKa = 5.9GTVSLAADD210 pKa = 4.25GSFTYY215 pKa = 10.34TPDD218 pKa = 3.51ADD220 pKa = 3.87YY221 pKa = 11.27SGPDD225 pKa = 3.06SFTYY229 pKa = 9.86KK230 pKa = 10.75AKK232 pKa = 10.84DD233 pKa = 3.36PGGLTSADD241 pKa = 3.56TTVSLTVTAVNDD253 pKa = 3.69APVAGDD259 pKa = 4.22DD260 pKa = 3.88SASTDD265 pKa = 3.13EE266 pKa = 4.31EE267 pKa = 4.49LAVDD271 pKa = 4.02VQVLEE276 pKa = 4.43NDD278 pKa = 3.55SDD280 pKa = 4.59PEE282 pKa = 4.3GSTLVVTGASGGTHH296 pKa = 5.94GTTAHH301 pKa = 7.0DD302 pKa = 4.41GDD304 pKa = 4.53SVTYY308 pKa = 9.64TPAPDD313 pKa = 3.61YY314 pKa = 11.01FGTDD318 pKa = 2.71SFTYY322 pKa = 10.22SVSDD326 pKa = 3.79GNGGTDD332 pKa = 3.07SATVTVTVSNVNDD345 pKa = 3.72APVAAGDD352 pKa = 3.78TGSTDD357 pKa = 3.17EE358 pKa = 5.6DD359 pKa = 3.79SALVVGASDD368 pKa = 3.96GLLANDD374 pKa = 4.26SDD376 pKa = 4.35VDD378 pKa = 4.02GDD380 pKa = 4.23SLTAALEE387 pKa = 4.61DD388 pKa = 4.62GPSHH392 pKa = 5.67GTVVLDD398 pKa = 3.63ADD400 pKa = 4.19GSYY403 pKa = 10.73SYY405 pKa = 10.93TPAADD410 pKa = 3.76YY411 pKa = 11.28YY412 pKa = 9.71GTDD415 pKa = 3.0SFTYY419 pKa = 9.72RR420 pKa = 11.84ANDD423 pKa = 3.79GAVSSGVATVTLTVAPVNDD442 pKa = 4.07APVADD447 pKa = 4.8DD448 pKa = 3.94EE449 pKa = 4.88SLTVAEE455 pKa = 5.11DD456 pKa = 3.49SQGSVDD462 pKa = 4.21VLLGDD467 pKa = 3.79TDD469 pKa = 4.1VEE471 pKa = 4.33GDD473 pKa = 3.86ALALTAVTEE482 pKa = 4.63GEE484 pKa = 4.26HH485 pKa = 5.71GTVVDD490 pKa = 4.31NGDD493 pKa = 3.38GTVTYY498 pKa = 10.0TPDD501 pKa = 3.4ADD503 pKa = 3.88YY504 pKa = 11.55AGADD508 pKa = 3.27SFTYY512 pKa = 8.18TVCDD516 pKa = 3.81DD517 pKa = 3.87HH518 pKa = 6.96ATTPTSSAGCDD529 pKa = 2.94TAMVEE534 pKa = 4.48VTVTPVNDD542 pKa = 3.82APVVTAGDD550 pKa = 3.48AASTDD555 pKa = 3.2EE556 pKa = 4.34GTAVTITATFTDD568 pKa = 3.13VDD570 pKa = 3.7RR571 pKa = 11.84AEE573 pKa = 4.73DD574 pKa = 3.55EE575 pKa = 4.74TYY577 pKa = 9.57TASIAWGDD585 pKa = 3.69GTTTAGTVAGNTVSGTHH602 pKa = 6.33TYY604 pKa = 10.99SDD606 pKa = 4.47DD607 pKa = 3.44EE608 pKa = 4.5AAPGDD613 pKa = 4.05DD614 pKa = 3.56VFPAVVTVTDD624 pKa = 3.54SGSTDD629 pKa = 3.32GVADD633 pKa = 4.41PLAGTATVAVTVANVAPTAATPALTVNPLTGLLSLSTSFSDD674 pKa = 4.0PAGSSDD680 pKa = 3.79TYY682 pKa = 11.03SGSFTVDD689 pKa = 2.75GDD691 pKa = 4.32TVPGTISGTTMSATTLLAPGCHH713 pKa = 5.02TVSATATVSDD723 pKa = 4.08EE724 pKa = 4.66DD725 pKa = 4.26GGSGTSGAGSVSQADD740 pKa = 4.02VYY742 pKa = 11.17AVSFQAPIRR751 pKa = 11.84DD752 pKa = 3.86DD753 pKa = 3.42VRR755 pKa = 11.84NVAKK759 pKa = 10.3YY760 pKa = 10.73GNVVPVKK767 pKa = 10.45VVITSSCTGLPVTTGPLAIQVVKK790 pKa = 11.11GNVTDD795 pKa = 5.03DD796 pKa = 3.63VTVDD800 pKa = 3.6DD801 pKa = 4.75SNAAVTEE808 pKa = 4.46SVSNADD814 pKa = 3.48SGTQMRR820 pKa = 11.84VADD823 pKa = 4.12GKK825 pKa = 11.03YY826 pKa = 9.81IYY828 pKa = 10.56NLSTKK833 pKa = 9.78GLTVGSDD840 pKa = 3.14YY841 pKa = 10.65TVRR844 pKa = 11.84IRR846 pKa = 11.84SGSTTGPVVLRR857 pKa = 11.84ALLSTKK863 pKa = 10.18KK864 pKa = 10.62

Molecular weight: 85.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T5WB69|A0A2T5WB69_9ACTN DNA integrity scanning protein DisA OS=Nocardioides sp. CF204 OX=2135677 GN=disA PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.72HH17 pKa = 5.74RR18 pKa = 11.84KK19 pKa = 7.77LLKK22 pKa = 8.07RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3302

0

3302

1042830

29

2969

315.8

33.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.451 ± 0.066

0.736 ± 0.01

6.183 ± 0.038

5.899 ± 0.039

2.919 ± 0.022

9.123 ± 0.036

2.21 ± 0.019

3.707 ± 0.03

2.428 ± 0.036

10.272 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.859 ± 0.018

1.886 ± 0.022

5.445 ± 0.034

2.794 ± 0.019

7.229 ± 0.045

5.1 ± 0.028

6.068 ± 0.041

9.193 ± 0.042

1.536 ± 0.018

1.962 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski