Amino acid dipepetide frequency for Nocardioides sp. CF204

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.356AlaAla: 20.356 ± 0.224
1.056AlaCys: 1.056 ± 0.035
8.347AlaAsp: 8.347 ± 0.109
8.355AlaGlu: 8.355 ± 0.157
3.837AlaPhe: 3.837 ± 0.058
12.601AlaGly: 12.601 ± 0.124
2.607AlaHis: 2.607 ± 0.056
4.761AlaIle: 4.761 ± 0.066
3.231AlaLys: 3.231 ± 0.07
13.833AlaLeu: 13.833 ± 0.181
2.941AlaMet: 2.941 ± 0.049
2.217AlaAsn: 2.217 ± 0.046
6.61AlaPro: 6.61 ± 0.121
3.684AlaGln: 3.684 ± 0.06
9.226AlaArg: 9.226 ± 0.107
6.616AlaSer: 6.616 ± 0.084
7.812AlaThr: 7.812 ± 0.098
11.504AlaVal: 11.504 ± 0.145
2.067AlaTrp: 2.067 ± 0.045
2.849AlaTyr: 2.849 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
0.944CysAla: 0.944 ± 0.03
0.081CysCys: 0.081 ± 0.009
0.488CysAsp: 0.488 ± 0.023
0.412CysGlu: 0.412 ± 0.017
0.211CysPhe: 0.211 ± 0.015
0.89CysGly: 0.89 ± 0.032
0.181CysHis: 0.181 ± 0.015
0.231CysIle: 0.231 ± 0.016
0.136CysLys: 0.136 ± 0.011
0.632CysLeu: 0.632 ± 0.023
0.123CysMet: 0.123 ± 0.01
0.152CysAsn: 0.152 ± 0.012
0.447CysPro: 0.447 ± 0.023
0.166CysGln: 0.166 ± 0.013
0.503CysArg: 0.503 ± 0.022
0.458CysSer: 0.458 ± 0.022
0.472CysThr: 0.472 ± 0.022
0.577CysVal: 0.577 ± 0.023
0.105CysTrp: 0.105 ± 0.011
0.154CysTyr: 0.154 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
8.053AspAla: 8.053 ± 0.094
0.403AspCys: 0.403 ± 0.021
4.058AspAsp: 4.058 ± 0.076
4.337AspGlu: 4.337 ± 0.074
1.715AspPhe: 1.715 ± 0.04
6.29AspGly: 6.29 ± 0.118
1.427AspHis: 1.427 ± 0.04
2.073AspIle: 2.073 ± 0.044
1.264AspLys: 1.264 ± 0.04
6.986AspLeu: 6.986 ± 0.076
0.896AspMet: 0.896 ± 0.032
1.085AspAsn: 1.085 ± 0.034
4.141AspPro: 4.141 ± 0.069
1.711AspGln: 1.711 ± 0.037
4.425AspArg: 4.425 ± 0.065
2.33AspSer: 2.33 ± 0.052
2.748AspThr: 2.748 ± 0.059
5.773AspVal: 5.773 ± 0.083
0.967AspTrp: 0.967 ± 0.028
1.145AspTyr: 1.145 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
7.836GluAla: 7.836 ± 0.113
0.347GluCys: 0.347 ± 0.021
2.777GluAsp: 2.777 ± 0.054
3.644GluGlu: 3.644 ± 0.074
1.679GluPhe: 1.679 ± 0.035
4.542GluGly: 4.542 ± 0.075
1.655GluHis: 1.655 ± 0.041
2.697GluIle: 2.697 ± 0.055
1.843GluLys: 1.843 ± 0.048
6.559GluLeu: 6.559 ± 0.089
1.084GluMet: 1.084 ± 0.032
1.029GluAsn: 1.029 ± 0.038
2.97GluPro: 2.97 ± 0.05
2.347GluGln: 2.347 ± 0.063
5.025GluArg: 5.025 ± 0.083
2.644GluSer: 2.644 ± 0.054
3.018GluThr: 3.018 ± 0.052
5.408GluVal: 5.408 ± 0.075
0.852GluTrp: 0.852 ± 0.03
1.035GluTyr: 1.035 ± 0.035
0.0GluXaa: 0.0 ± 0.0
Phe
3.897PheAla: 3.897 ± 0.064
0.274PheCys: 0.274 ± 0.017
2.103PheAsp: 2.103 ± 0.051
1.65PheGlu: 1.65 ± 0.04
0.925PhePhe: 0.925 ± 0.031
3.234PheGly: 3.234 ± 0.056
0.684PheHis: 0.684 ± 0.023
0.879PheIle: 0.879 ± 0.029
0.628PheLys: 0.628 ± 0.025
2.779PheLeu: 2.779 ± 0.062
0.478PheMet: 0.478 ± 0.025
0.669PheAsn: 0.669 ± 0.027
1.368PhePro: 1.368 ± 0.04
0.707PheGln: 0.707 ± 0.024
1.856PheArg: 1.856 ± 0.047
1.461PheSer: 1.461 ± 0.04
1.889PheThr: 1.889 ± 0.044
2.678PheVal: 2.678 ± 0.05
0.428PheTrp: 0.428 ± 0.021
0.599PheTyr: 0.599 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
10.666GlyAla: 10.666 ± 0.125
0.811GlyCys: 0.811 ± 0.029
5.213GlyAsp: 5.213 ± 0.085
5.18GlyGlu: 5.18 ± 0.068
3.168GlyPhe: 3.168 ± 0.058
8.122GlyGly: 8.122 ± 0.126
2.148GlyHis: 2.148 ± 0.053
3.92GlyIle: 3.92 ± 0.061
2.528GlyLys: 2.528 ± 0.054
9.348GlyLeu: 9.348 ± 0.115
1.944GlyMet: 1.944 ± 0.044
1.667GlyAsn: 1.667 ± 0.046
4.18GlyPro: 4.18 ± 0.072
2.673GlyGln: 2.673 ± 0.057
6.537GlyArg: 6.537 ± 0.081
5.358GlySer: 5.358 ± 0.077
5.826GlyThr: 5.826 ± 0.128
8.084GlyVal: 8.084 ± 0.089
1.739GlyTrp: 1.739 ± 0.043
2.118GlyTyr: 2.118 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
2.675HisAla: 2.675 ± 0.055
0.171HisCys: 0.171 ± 0.012
1.551HisAsp: 1.551 ± 0.041
1.281HisGlu: 1.281 ± 0.03
0.661HisPhe: 0.661 ± 0.023
2.215HisGly: 2.215 ± 0.047
0.703HisHis: 0.703 ± 0.028
0.604HisIle: 0.604 ± 0.025
0.385HisLys: 0.385 ± 0.022
2.44HisLeu: 2.44 ± 0.051
0.352HisMet: 0.352 ± 0.019
0.392HisAsn: 0.392 ± 0.02
1.535HisPro: 1.535 ± 0.04
0.639HisGln: 0.639 ± 0.024
1.691HisArg: 1.691 ± 0.04
0.861HisSer: 0.861 ± 0.029
1.146HisThr: 1.146 ± 0.031
2.028HisVal: 2.028 ± 0.044
0.344HisTrp: 0.344 ± 0.023
0.425HisTyr: 0.425 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
5.503IleAla: 5.503 ± 0.07
0.292IleCys: 0.292 ± 0.016
2.878IleAsp: 2.878 ± 0.047
2.585IleGlu: 2.585 ± 0.055
0.87IlePhe: 0.87 ± 0.033
3.902IleGly: 3.902 ± 0.069
0.776IleHis: 0.776 ± 0.027
1.177IleIle: 1.177 ± 0.037
0.965IleLys: 0.965 ± 0.035
2.787IleLeu: 2.787 ± 0.051
0.499IleMet: 0.499 ± 0.024
0.922IleAsn: 0.922 ± 0.034
1.942IlePro: 1.942 ± 0.045
0.852IleGln: 0.852 ± 0.03
2.41IleArg: 2.41 ± 0.055
1.911IleSer: 1.911 ± 0.047
2.435IleThr: 2.435 ± 0.051
3.351IleVal: 3.351 ± 0.057
0.434IleTrp: 0.434 ± 0.021
0.587IleTyr: 0.587 ± 0.023
0.0IleXaa: 0.0 ± 0.0
Lys
3.358LysAla: 3.358 ± 0.076
0.117LysCys: 0.117 ± 0.011
1.29LysAsp: 1.29 ± 0.041
1.329LysGlu: 1.329 ± 0.041
0.631LysPhe: 0.631 ± 0.026
2.09LysGly: 2.09 ± 0.053
0.478LysHis: 0.478 ± 0.018
1.041LysIle: 1.041 ± 0.034
1.045LysLys: 1.045 ± 0.051
2.134LysLeu: 2.134 ± 0.049
0.462LysMet: 0.462 ± 0.021
0.541LysAsn: 0.541 ± 0.025
1.497LysPro: 1.497 ± 0.046
0.779LysGln: 0.779 ± 0.027
1.663LysArg: 1.663 ± 0.043
1.219LysSer: 1.219 ± 0.039
1.383LysThr: 1.383 ± 0.037
2.475LysVal: 2.475 ± 0.055
0.259LysTrp: 0.259 ± 0.016
0.494LysTyr: 0.494 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
15.295LeuAla: 15.295 ± 0.163
0.721LeuCys: 0.721 ± 0.026
6.827LeuAsp: 6.827 ± 0.092
5.68LeuGlu: 5.68 ± 0.076
2.626LeuPhe: 2.626 ± 0.055
9.563LeuGly: 9.563 ± 0.112
2.125LeuHis: 2.125 ± 0.043
3.206LeuIle: 3.206 ± 0.067
2.126LeuLys: 2.126 ± 0.045
10.915LeuLeu: 10.915 ± 0.153
1.718LeuMet: 1.718 ± 0.042
1.708LeuAsn: 1.708 ± 0.046
5.803LeuPro: 5.803 ± 0.079
2.557LeuGln: 2.557 ± 0.047
7.454LeuArg: 7.454 ± 0.093
5.06LeuSer: 5.06 ± 0.066
6.335LeuThr: 6.335 ± 0.081
10.131LeuVal: 10.131 ± 0.12
1.273LeuTrp: 1.273 ± 0.033
1.6LeuTyr: 1.6 ± 0.038
0.0LeuXaa: 0.0 ± 0.0
Met
2.413MetAla: 2.413 ± 0.046
0.144MetCys: 0.144 ± 0.011
0.879MetAsp: 0.879 ± 0.029
0.853MetGlu: 0.853 ± 0.031
0.492MetPhe: 0.492 ± 0.021
1.4MetGly: 1.4 ± 0.037
0.365MetHis: 0.365 ± 0.019
0.678MetIle: 0.678 ± 0.023
0.564MetLys: 0.564 ± 0.022
1.912MetLeu: 1.912 ± 0.044
0.312MetMet: 0.312 ± 0.019
0.471MetAsn: 0.471 ± 0.022
1.046MetPro: 1.046 ± 0.029
0.525MetGln: 0.525 ± 0.024
1.334MetArg: 1.334 ± 0.038
1.456MetSer: 1.456 ± 0.033
1.776MetThr: 1.776 ± 0.038
1.495MetVal: 1.495 ± 0.037
0.2MetTrp: 0.2 ± 0.014
0.27MetTyr: 0.27 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
2.393AsnAla: 2.393 ± 0.049
0.155AsnCys: 0.155 ± 0.013
1.199AsnAsp: 1.199 ± 0.039
0.908AsnGlu: 0.908 ± 0.034
0.54AsnPhe: 0.54 ± 0.026
1.918AsnGly: 1.918 ± 0.048
0.434AsnHis: 0.434 ± 0.023
0.701AsnIle: 0.701 ± 0.028
0.494AsnLys: 0.494 ± 0.024
1.943AsnLeu: 1.943 ± 0.039
0.312AsnMet: 0.312 ± 0.02
0.453AsnAsn: 0.453 ± 0.021
1.45AsnPro: 1.45 ± 0.043
0.553AsnGln: 0.553 ± 0.024
1.271AsnArg: 1.271 ± 0.033
0.808AsnSer: 0.808 ± 0.035
1.048AsnThr: 1.048 ± 0.036
1.599AsnVal: 1.599 ± 0.046
0.289AsnTrp: 0.289 ± 0.018
0.395AsnTyr: 0.395 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
7.94ProAla: 7.94 ± 0.128
0.292ProCys: 0.292 ± 0.017
3.913ProAsp: 3.913 ± 0.064
3.913ProGlu: 3.913 ± 0.06
1.601ProPhe: 1.601 ± 0.041
5.251ProGly: 5.251 ± 0.09
1.214ProHis: 1.214 ± 0.031
1.669ProIle: 1.669 ± 0.042
1.266ProLys: 1.266 ± 0.045
4.903ProLeu: 4.903 ± 0.066
0.954ProMet: 0.954 ± 0.03
0.928ProAsn: 0.928 ± 0.029
2.327ProPro: 2.327 ± 0.061
1.434ProGln: 1.434 ± 0.036
3.343ProArg: 3.343 ± 0.057
2.882ProSer: 2.882 ± 0.054
3.407ProThr: 3.407 ± 0.066
5.221ProVal: 5.221 ± 0.083
0.882ProTrp: 0.882 ± 0.027
1.112ProTyr: 1.112 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
3.666GlnAla: 3.666 ± 0.08
0.185GlnCys: 0.185 ± 0.013
1.158GlnAsp: 1.158 ± 0.036
1.472GlnGlu: 1.472 ± 0.036
0.738GlnPhe: 0.738 ± 0.026
2.152GlnGly: 2.152 ± 0.047
0.675GlnHis: 0.675 ± 0.021
1.171GlnIle: 1.171 ± 0.032
0.729GlnLys: 0.729 ± 0.028
3.19GlnLeu: 3.19 ± 0.065
0.533GlnMet: 0.533 ± 0.021
0.495GlnAsn: 0.495 ± 0.023
1.661GlnPro: 1.661 ± 0.041
1.138GlnGln: 1.138 ± 0.037
2.446GlnArg: 2.446 ± 0.051
1.232GlnSer: 1.232 ± 0.035
1.41GlnThr: 1.41 ± 0.039
2.879GlnVal: 2.879 ± 0.056
0.452GlnTrp: 0.452 ± 0.022
0.554GlnTyr: 0.554 ± 0.022
0.0GlnXaa: 0.0 ± 0.0
Arg
8.438ArgAla: 8.438 ± 0.116
0.471ArgCys: 0.471 ± 0.02
4.249ArgAsp: 4.249 ± 0.073
4.47ArgGlu: 4.47 ± 0.069
2.264ArgPhe: 2.264 ± 0.049
5.34ArgGly: 5.34 ± 0.072
1.736ArgHis: 1.736 ± 0.046
3.27ArgIle: 3.27 ± 0.057
1.763ArgLys: 1.763 ± 0.045
7.676ArgLeu: 7.676 ± 0.097
1.704ArgMet: 1.704 ± 0.042
1.371ArgAsn: 1.371 ± 0.033
3.843ArgPro: 3.843 ± 0.061
2.113ArgGln: 2.113 ± 0.048
6.54ArgArg: 6.54 ± 0.114
3.877ArgSer: 3.877 ± 0.064
4.463ArgThr: 4.463 ± 0.064
5.97ArgVal: 5.97 ± 0.066
1.251ArgTrp: 1.251 ± 0.035
1.483ArgTyr: 1.483 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
6.39SerAla: 6.39 ± 0.079
0.373SerCys: 0.373 ± 0.02
2.89SerAsp: 2.89 ± 0.056
2.533SerGlu: 2.533 ± 0.053
1.674SerPhe: 1.674 ± 0.042
5.435SerGly: 5.435 ± 0.078
1.042SerHis: 1.042 ± 0.028
1.898SerIle: 1.898 ± 0.048
1.177SerLys: 1.177 ± 0.033
5.01SerLeu: 5.01 ± 0.074
1.153SerMet: 1.153 ± 0.034
0.999SerAsn: 0.999 ± 0.031
2.854SerPro: 2.854 ± 0.06
1.3SerGln: 1.3 ± 0.035
3.518SerArg: 3.518 ± 0.066
2.972SerSer: 2.972 ± 0.065
3.408SerThr: 3.408 ± 0.063
4.27SerVal: 4.27 ± 0.072
0.905SerTrp: 0.905 ± 0.026
1.196SerTyr: 1.196 ± 0.037
0.0SerXaa: 0.0 ± 0.0
Thr
7.716ThrAla: 7.716 ± 0.1
0.494ThrCys: 0.494 ± 0.024
3.605ThrAsp: 3.605 ± 0.098
3.004ThrGlu: 3.004 ± 0.059
1.88ThrPhe: 1.88 ± 0.047
6.007ThrGly: 6.007 ± 0.084
1.191ThrHis: 1.191 ± 0.031
2.375ThrIle: 2.375 ± 0.05
1.322ThrLys: 1.322 ± 0.037
5.867ThrLeu: 5.867 ± 0.085
1.017ThrMet: 1.017 ± 0.03
1.165ThrAsn: 1.165 ± 0.036
4.084ThrPro: 4.084 ± 0.082
1.496ThrGln: 1.496 ± 0.034
3.765ThrArg: 3.765 ± 0.053
3.471ThrSer: 3.471 ± 0.069
4.092ThrThr: 4.092 ± 0.084
5.615ThrVal: 5.615 ± 0.087
1.099ThrTrp: 1.099 ± 0.034
1.42ThrTyr: 1.42 ± 0.036
0.0ThrXaa: 0.0 ± 0.0
Val
12.487ValAla: 12.487 ± 0.135
0.657ValCys: 0.657 ± 0.023
5.941ValAsp: 5.941 ± 0.079
5.614ValGlu: 5.614 ± 0.082
2.429ValPhe: 2.429 ± 0.048
7.357ValGly: 7.357 ± 0.1
1.957ValHis: 1.957 ± 0.043
3.644ValIle: 3.644 ± 0.064
1.996ValLys: 1.996 ± 0.048
9.822ValLeu: 9.822 ± 0.111
1.54ValMet: 1.54 ± 0.041
1.821ValAsn: 1.821 ± 0.048
5.03ValPro: 5.03 ± 0.064
2.172ValGln: 2.172 ± 0.047
6.595ValArg: 6.595 ± 0.092
4.475ValSer: 4.475 ± 0.065
5.974ValThr: 5.974 ± 0.088
9.783ValVal: 9.783 ± 0.122
1.156ValTrp: 1.156 ± 0.034
1.478ValTyr: 1.478 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.626TrpAla: 1.626 ± 0.037
0.159TrpCys: 0.159 ± 0.013
0.804TrpAsp: 0.804 ± 0.027
0.742TrpGlu: 0.742 ± 0.027
0.585TrpPhe: 0.585 ± 0.027
1.073TrpGly: 1.073 ± 0.031
0.407TrpHis: 0.407 ± 0.022
0.582TrpIle: 0.582 ± 0.024
0.422TrpLys: 0.422 ± 0.019
1.956TrpLeu: 1.956 ± 0.054
0.315TrpMet: 0.315 ± 0.016
0.372TrpAsn: 0.372 ± 0.023
0.727TrpPro: 0.727 ± 0.026
0.589TrpGln: 0.589 ± 0.024
1.209TrpArg: 1.209 ± 0.034
1.008TrpSer: 1.008 ± 0.034
0.978TrpThr: 0.978 ± 0.028
1.159TrpVal: 1.159 ± 0.034
0.35TrpTrp: 0.35 ± 0.018
0.292TrpTyr: 0.292 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.858TyrAla: 2.858 ± 0.057
0.161TyrCys: 0.161 ± 0.013
1.657TyrAsp: 1.657 ± 0.039
1.025TyrGlu: 1.025 ± 0.026
0.662TyrPhe: 0.662 ± 0.026
1.846TyrGly: 1.846 ± 0.045
0.294TyrHis: 0.294 ± 0.016
0.498TyrIle: 0.498 ± 0.025
0.4TyrLys: 0.4 ± 0.019
2.03TyrLeu: 2.03 ± 0.046
0.249TyrMet: 0.249 ± 0.016
0.406TyrAsn: 0.406 ± 0.021
0.939TyrPro: 0.939 ± 0.034
0.505TyrGln: 0.505 ± 0.025
1.483TyrArg: 1.483 ± 0.044
0.899TyrSer: 0.899 ± 0.033
1.064TyrThr: 1.064 ± 0.033
1.926TyrVal: 1.926 ± 0.04
0.305TyrTrp: 0.305 ± 0.017
0.415TyrTyr: 0.415 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3302 proteins (1042830 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski