Saccharicrinis carchari

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Marinilabiliales; Marinilabiliaceae; Saccharicrinis

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3615 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A521FBV8|A0A521FBV8_9BACT DNA helicase OS=Saccharicrinis carchari OX=1168039 GN=SAMN06265379_1209 PE=4 SV=1
MM1 pKa = 7.21YY2 pKa = 10.47KK3 pKa = 10.41SIIYY7 pKa = 8.97GFSMLLALSSCEE19 pKa = 3.97EE20 pKa = 4.01VNMLQLGEE28 pKa = 4.27TVDD31 pKa = 4.59LVLSSDD37 pKa = 3.93VVGKK41 pKa = 10.59SIVLSEE47 pKa = 5.4DD48 pKa = 2.84IAEE51 pKa = 4.28EE52 pKa = 4.0PFGAFTWTPADD63 pKa = 3.58FGYY66 pKa = 10.17PSASPEE72 pKa = 4.34YY73 pKa = 9.21ILQMDD78 pKa = 4.25FEE80 pKa = 6.19GNDD83 pKa = 3.41FKK85 pKa = 11.66NAITLINTLDD95 pKa = 3.61LRR97 pKa = 11.84FEE99 pKa = 4.28NTNAMFNQKK108 pKa = 10.37LITLGAVPGVASNLEE123 pKa = 4.07FKK125 pKa = 10.3LTGKK129 pKa = 9.73LHH131 pKa = 6.0EE132 pKa = 4.41EE133 pKa = 4.67VYY135 pKa = 11.01AVGNVLTANITPYY148 pKa = 10.39KK149 pKa = 9.52IVPVFPKK156 pKa = 10.52LYY158 pKa = 9.47VTGDD162 pKa = 3.16HH163 pKa = 5.94NTWGFNDD170 pKa = 3.38EE171 pKa = 4.15SAIFSVKK178 pKa = 10.59DD179 pKa = 3.17NAIYY183 pKa = 10.25EE184 pKa = 4.48GYY186 pKa = 10.13IYY188 pKa = 10.6LRR190 pKa = 11.84EE191 pKa = 4.57DD192 pKa = 3.45GLIKK196 pKa = 10.74FSQQPNWDD204 pKa = 3.86NPNAIIGDD212 pKa = 4.05LDD214 pKa = 3.61EE215 pKa = 5.83SGTSGVLQIGNWGGNNIVASQAAGVYY241 pKa = 10.46KK242 pKa = 10.49FMVNLNNQSYY252 pKa = 10.17SQLLVNWAVTGDD264 pKa = 3.75FNSWAMADD272 pKa = 3.47MVYY275 pKa = 10.6DD276 pKa = 3.67ITTGLWSLTVDD287 pKa = 3.59MTAGGFKK294 pKa = 10.55FIANQDD300 pKa = 3.01WGKK303 pKa = 11.15VYY305 pKa = 10.84GDD307 pKa = 4.54DD308 pKa = 4.74EE309 pKa = 4.55MDD311 pKa = 3.55GVLDD315 pKa = 4.93KK316 pKa = 11.57GSDD319 pKa = 3.48GNNIIIQEE327 pKa = 4.25GGNYY331 pKa = 8.87TITMDD336 pKa = 5.17LSDD339 pKa = 4.31ALYY342 pKa = 9.64TYY344 pKa = 10.72SIVKK348 pKa = 10.38NNN350 pKa = 3.14

Molecular weight: 38.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A521EPP5|A0A521EPP5_9BACT Uncharacterized protein OS=Saccharicrinis carchari OX=1168039 GN=SAMN06265379_11072 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84KK11 pKa = 8.89RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.84HH16 pKa = 3.75GFRR19 pKa = 11.84ARR21 pKa = 11.84MASANGRR28 pKa = 11.84KK29 pKa = 9.04VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.02GRR39 pKa = 11.84KK40 pKa = 8.41KK41 pKa = 10.8LSVSCEE47 pKa = 3.86KK48 pKa = 10.5RR49 pKa = 11.84HH50 pKa = 6.22KK51 pKa = 10.58LL52 pKa = 3.64

Molecular weight: 6.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3615

0

3615

1343413

29

6488

371.6

41.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.792 ± 0.036

0.831 ± 0.017

5.506 ± 0.032

6.149 ± 0.043

4.892 ± 0.032

6.793 ± 0.052

1.984 ± 0.019

7.486 ± 0.041

7.272 ± 0.057

9.321 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.544 ± 0.02

5.73 ± 0.043

3.622 ± 0.025

3.588 ± 0.022

3.874 ± 0.029

6.428 ± 0.034

5.356 ± 0.055

6.527 ± 0.034

1.166 ± 0.017

4.141 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski