Capybara microvirus Cap3_SP_389
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVS8|A0A4V1FVS8_9VIRU Nonstructural protein OS=Capybara microvirus Cap3_SP_389 OX=2585441 PE=4 SV=1
MM1 pKa = 7.07 NRR3 pKa = 11.84 EE4 pKa = 4.14 SNNYY8 pKa = 9.57 FSDD11 pKa = 3.55 LPYY14 pKa = 10.14 IEE16 pKa = 4.71 KK17 pKa = 10.15 PRR19 pKa = 11.84 SKK21 pKa = 10.33 FSRR24 pKa = 11.84 PQPHH28 pKa = 5.9 ITTFKK33 pKa = 10.86 NGDD36 pKa = 4.55 LIPVYY41 pKa = 9.98 IDD43 pKa = 3.17 EE44 pKa = 4.58 VLPGDD49 pKa = 4.78 SMTIDD54 pKa = 3.28 VNAVVRR60 pKa = 11.84 MMTPIYY66 pKa = 10.2 PVMDD70 pKa = 4.1 NLILDD75 pKa = 3.89 VMAFYY80 pKa = 10.88 CPNRR84 pKa = 11.84 LLWTHH89 pKa = 4.94 WQEE92 pKa = 4.4 FLGEE96 pKa = 4.19 NKK98 pKa = 8.45 LTAWEE103 pKa = 4.19 QPVKK107 pKa = 11.25 YY108 pKa = 9.57 EE109 pKa = 3.87 IPQIKK114 pKa = 9.94 APEE117 pKa = 4.66 GGWKK121 pKa = 10.34 AGTIADD127 pKa = 3.79 YY128 pKa = 11.15 LGIPTYY134 pKa = 11.05 VEE136 pKa = 4.0 NLSVNAMPFRR146 pKa = 11.84 AYY148 pKa = 10.96 SLIWNEE154 pKa = 3.58 FFRR157 pKa = 11.84 DD158 pKa = 3.48 EE159 pKa = 4.1 NLKK162 pKa = 10.8 DD163 pKa = 3.19 RR164 pKa = 11.84 VMINLDD170 pKa = 3.14 DD171 pKa = 3.82 ATRR174 pKa = 11.84 VGSNGDD180 pKa = 3.63 KK181 pKa = 11.19 EE182 pKa = 4.25 GDD184 pKa = 3.82 TYY186 pKa = 11.14 VTTAQLGAKK195 pKa = 8.06 PLKK198 pKa = 9.47 VAKK201 pKa = 8.98 THH203 pKa = 7.73 DD204 pKa = 4.56 YY205 pKa = 7.87 FTSALPNTQKK215 pKa = 11.19 GEE217 pKa = 3.89 AVLIPVISAGTGGLIPVTAGEE238 pKa = 3.97 RR239 pKa = 11.84 HH240 pKa = 5.91 IEE242 pKa = 4.16 NGSVTGNGLLWATNQSGLNNWWNVQYY268 pKa = 11.21 DD269 pKa = 4.01 GLSDD273 pKa = 3.57 NKK275 pKa = 9.88 TVSTAATGGTIHH287 pKa = 6.84 NGPIVPANLYY297 pKa = 11.13 ANLEE301 pKa = 4.31 DD302 pKa = 4.04 SQSLATIGTINEE314 pKa = 4.03 LRR316 pKa = 11.84 QSFAIQRR323 pKa = 11.84 ILEE326 pKa = 4.04 AMARR330 pKa = 11.84 GGSRR334 pKa = 11.84 YY335 pKa = 8.13 TEE337 pKa = 3.59 ILKK340 pKa = 10.33 NIWGVTAPDD349 pKa = 3.5 ARR351 pKa = 11.84 LQRR354 pKa = 11.84 PEE356 pKa = 3.86 YY357 pKa = 10.42 LGGEE361 pKa = 4.55 RR362 pKa = 11.84 IPINMDD368 pKa = 2.95 QVLQTSASNGNNPLGDD384 pKa = 3.12 TGAFSNTGFSTTLVNKK400 pKa = 10.26 SFVEE404 pKa = 3.78 HH405 pKa = 6.74 GYY407 pKa = 10.71 VIIVAAARR415 pKa = 11.84 IRR417 pKa = 11.84 NHH419 pKa = 6.76 TYY421 pKa = 9.55 QQGLEE426 pKa = 4.24 KK427 pKa = 9.25 MWSRR431 pKa = 11.84 KK432 pKa = 7.37 TMYY435 pKa = 10.35 DD436 pKa = 3.01 FYY438 pKa = 10.36 MPQLAYY444 pKa = 10.66 LSEE447 pKa = 3.91 QAVLNKK453 pKa = 10.13 EE454 pKa = 4.09 IYY456 pKa = 9.85 AQGNRR461 pKa = 11.84 EE462 pKa = 4.0 DD463 pKa = 4.32 EE464 pKa = 4.39 EE465 pKa = 4.74 VFGYY469 pKa = 7.43 QEE471 pKa = 3.15 RR472 pKa = 11.84 WAEE475 pKa = 3.82 YY476 pKa = 8.27 RR477 pKa = 11.84 YY478 pKa = 10.64 KK479 pKa = 10.45 PGRR482 pKa = 11.84 VSGAMRR488 pKa = 11.84 SNYY491 pKa = 9.61 PNGSLDD497 pKa = 3.19 SWHH500 pKa = 6.46 YY501 pKa = 11.42 ADD503 pKa = 6.01 DD504 pKa = 4.23 YY505 pKa = 11.23 VSKK508 pKa = 10.74 PMLSSEE514 pKa = 4.41 WIDD517 pKa = 3.43 EE518 pKa = 4.28 GTSEE522 pKa = 4.47 VDD524 pKa = 3.01 RR525 pKa = 11.84 TIAIQSEE532 pKa = 4.48 LEE534 pKa = 4.13 DD535 pKa = 3.4 QFLADD540 pKa = 5.28 FLFINNSTRR549 pKa = 11.84 TMPIYY554 pKa = 10.38 SVPGLLDD561 pKa = 3.54 HH562 pKa = 6.83 YY563 pKa = 11.48
Molecular weight: 63.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.913
IPC2_protein 4.991
IPC_protein 4.94
Toseland 4.851
ProMoST 5.067
Dawson 4.914
Bjellqvist 5.041
Wikipedia 4.774
Rodwell 4.813
Grimsley 4.762
Solomon 4.914
Lehninger 4.863
Nozaki 5.029
DTASelect 5.181
Thurlkill 4.838
EMBOSS 4.813
Sillero 5.092
Patrickios 3.795
IPC_peptide 4.914
IPC2_peptide 5.092
IPC2.peptide.svr19 5.041
Protein with the highest isoelectric point:
>tr|A0A4P8W8G2|A0A4P8W8G2_9VIRU Lysozyme OS=Capybara microvirus Cap3_SP_389 OX=2585441 PE=3 SV=1
MM1 pKa = 7.5 SAIACIKK8 pKa = 9.61 PIKK11 pKa = 9.94 CWEE14 pKa = 4.04 HH15 pKa = 5.92 LKK17 pKa = 10.94 KK18 pKa = 9.88 KK19 pKa = 6.36 TAKK22 pKa = 9.31 GRR24 pKa = 11.84 KK25 pKa = 8.83 LISFKK30 pKa = 10.9 QPTISDD36 pKa = 3.13 IDD38 pKa = 3.48 NWNEE42 pKa = 3.46 IQIPCGQCIEE52 pKa = 4.09 CRR54 pKa = 11.84 LRR56 pKa = 11.84 YY57 pKa = 8.9 SRR59 pKa = 11.84 HH60 pKa = 4.07 WATRR64 pKa = 11.84 LTLEE68 pKa = 4.42 KK69 pKa = 10.11 KK70 pKa = 7.22 YY71 pKa = 10.78 HH72 pKa = 6.08 KK73 pKa = 10.05 YY74 pKa = 10.85 SYY76 pKa = 10.37 FITLTYY82 pKa = 10.68 DD83 pKa = 3.11 EE84 pKa = 4.66 EE85 pKa = 4.48 HH86 pKa = 6.23 LHH88 pKa = 5.74 RR89 pKa = 11.84 VEE91 pKa = 5.78 NIDD94 pKa = 3.55 TKK96 pKa = 10.4 TGEE99 pKa = 4.05 IVEE102 pKa = 4.68 LSTLVKK108 pKa = 10.46 KK109 pKa = 10.78 DD110 pKa = 3.17 MQDD113 pKa = 2.85 FLKK116 pKa = 10.34 RR117 pKa = 11.84 YY118 pKa = 8.2 RR119 pKa = 11.84 YY120 pKa = 9.79 YY121 pKa = 9.45 YY122 pKa = 10.98 GKK124 pKa = 10.09 IKK126 pKa = 10.42 FYY128 pKa = 10.75 QCGEE132 pKa = 4.22 YY133 pKa = 10.92 GEE135 pKa = 4.37 QTHH138 pKa = 6.38 RR139 pKa = 11.84 RR140 pKa = 11.84 HH141 pKa = 5.26 HH142 pKa = 6.08 HH143 pKa = 5.6 MIVFQEE149 pKa = 4.27 RR150 pKa = 11.84 PIEE153 pKa = 3.99 PLEE156 pKa = 3.79 FLKK159 pKa = 10.74 LQKK162 pKa = 10.54 DD163 pKa = 3.8 NQPLYY168 pKa = 11.05 TNLPLQKK175 pKa = 9.5 IWGKK179 pKa = 10.76 GMITIGEE186 pKa = 4.31 VTPEE190 pKa = 3.63 SRR192 pKa = 11.84 GYY194 pKa = 8.08 VARR197 pKa = 11.84 YY198 pKa = 8.61 VLKK201 pKa = 9.91 KK202 pKa = 10.65 QKK204 pKa = 10.78 GEE206 pKa = 3.81 SSNIYY211 pKa = 8.28 NTLGILPEE219 pKa = 4.27 YY220 pKa = 9.78 VTMSNGIGMEE230 pKa = 3.95 YY231 pKa = 10.62 FKK233 pKa = 11.29 DD234 pKa = 3.73 NKK236 pKa = 9.2 TKK238 pKa = 10.13 IYY240 pKa = 10.18 QYY242 pKa = 11.47 DD243 pKa = 4.0 SIILTGMHH251 pKa = 6.65 GEE253 pKa = 4.07 AKK255 pKa = 9.83 KK256 pKa = 10.69 VKK258 pKa = 9.4 PPRR261 pKa = 11.84 AFDD264 pKa = 3.49 EE265 pKa = 4.41 EE266 pKa = 4.3 LAKK269 pKa = 10.98 EE270 pKa = 4.01 NLSEE274 pKa = 3.95 ITSIKK279 pKa = 10.1 DD280 pKa = 3.0 KK281 pKa = 10.84 RR282 pKa = 11.84 RR283 pKa = 11.84 AAAIRR288 pKa = 11.84 GRR290 pKa = 11.84 SVKK293 pKa = 9.63 EE294 pKa = 3.63 AQTEE298 pKa = 4.1 NVNWAKK304 pKa = 10.75 VEE306 pKa = 3.94 SDD308 pKa = 3.55 YY309 pKa = 11.55 KK310 pKa = 10.2 QARR313 pKa = 11.84 QKK315 pKa = 10.5 ILNKK319 pKa = 10.31 RR320 pKa = 11.84 EE321 pKa = 3.61 VDD323 pKa = 3.71 DD324 pKa = 4.16
Molecular weight: 38.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.764
IPC2_protein 8.741
IPC_protein 8.609
Toseland 9.311
ProMoST 9.121
Dawson 9.604
Bjellqvist 9.355
Wikipedia 9.78
Rodwell 9.882
Grimsley 9.677
Solomon 9.633
Lehninger 9.589
Nozaki 9.458
DTASelect 9.311
Thurlkill 9.458
EMBOSS 9.75
Sillero 9.56
Patrickios 4.724
IPC_peptide 9.633
IPC2_peptide 7.951
IPC2.peptide.svr19 7.799
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1605
74
563
229.3
26.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.854 ± 1.486
0.748 ± 0.334
5.483 ± 0.421
7.975 ± 0.86
3.427 ± 0.43
6.729 ± 0.502
1.62 ± 0.424
7.539 ± 0.74
7.477 ± 1.537
7.04 ± 0.647
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.617 ± 0.327
5.919 ± 0.785
3.614 ± 0.712
3.988 ± 0.214
5.234 ± 0.286
6.231 ± 0.969
6.355 ± 0.463
4.361 ± 0.585
1.807 ± 0.243
4.984 ± 0.425
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here