Caviid betaherpesvirus 2
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B7TPW2|B7TPW2_9BETA GP43 OS=Caviid betaherpesvirus 2 OX=33706 GN=GP43 PE=4 SV=1
MM1 pKa = 7.94 DD2 pKa = 4.95 GAIEE6 pKa = 4.29 SYY8 pKa = 10.61 EE9 pKa = 4.36 LDD11 pKa = 3.26 VLGYY15 pKa = 10.75 VSGDD19 pKa = 3.29 PEE21 pKa = 4.22 GRR23 pKa = 11.84 QPYY26 pKa = 10.11 AEE28 pKa = 4.49 PPPYY32 pKa = 10.33 FEE34 pKa = 4.9 TVLEE38 pKa = 4.3 SHH40 pKa = 7.45 LDD42 pKa = 3.55 PPPDD46 pKa = 3.67 YY47 pKa = 11.35 FEE49 pKa = 4.65 VCHH52 pKa = 6.48 DD53 pKa = 3.84 ASDD56 pKa = 3.7 AVSLYY61 pKa = 10.32 RR62 pKa = 11.84 YY63 pKa = 9.38 DD64 pKa = 3.03 SRR66 pKa = 11.84 VILRR70 pKa = 11.84 QSVCDD75 pKa = 4.02 DD76 pKa = 4.11 DD77 pKa = 4.85 EE78 pKa = 5.39 DD79 pKa = 4.59 RR80 pKa = 11.84 SARR83 pKa = 11.84 NGVKK87 pKa = 10.07 HH88 pKa = 5.88 CVCCAISVLMLILLLVAFGTLMKK111 pKa = 10.45 SSLSIVRR118 pKa = 11.84 GEE120 pKa = 3.89 NN121 pKa = 3.02
Molecular weight: 13.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.062
IPC2_protein 4.304
IPC_protein 4.228
Toseland 4.05
ProMoST 4.368
Dawson 4.202
Bjellqvist 4.355
Wikipedia 4.113
Rodwell 4.062
Grimsley 3.961
Solomon 4.202
Lehninger 4.151
Nozaki 4.317
DTASelect 4.52
Thurlkill 4.075
EMBOSS 4.126
Sillero 4.355
Patrickios 0.807
IPC_peptide 4.202
IPC2_peptide 4.329
IPC2.peptide.svr19 4.245
Protein with the highest isoelectric point:
>tr|B7TQ21|B7TQ21_9BETA Gp141 OS=Caviid betaherpesvirus 2 OX=33706 GN=gp141 PE=4 SV=1
MM1 pKa = 7.59 ACMNLLYY8 pKa = 10.69 ASLLSKK14 pKa = 10.42 IGSSLSFRR22 pKa = 11.84 WYY24 pKa = 10.87 SEE26 pKa = 3.63 TFRR29 pKa = 11.84 NLKK32 pKa = 10.37 AAMSASSSLRR42 pKa = 11.84 DD43 pKa = 3.4 GRR45 pKa = 11.84 FVSSIRR51 pKa = 11.84 TLSWVEE57 pKa = 3.68 LYY59 pKa = 10.95 VILSHH64 pKa = 5.77 SHH66 pKa = 5.17 SNARR70 pKa = 11.84 ADD72 pKa = 3.55 SSLSATGRR80 pKa = 11.84 PASTSSTLSVIAEE93 pKa = 4.17 PLFPPPNARR102 pKa = 11.84 SSGRR106 pKa = 11.84 ARR108 pKa = 11.84 SSFPGTEE115 pKa = 3.77 ANYY118 pKa = 8.64 TCIYY122 pKa = 9.72 RR123 pKa = 11.84 PRR125 pKa = 11.84 TSRR128 pKa = 11.84 CPEE131 pKa = 3.46 IPVRR135 pKa = 4.24
Molecular weight: 14.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 9.736
IPC_protein 10.716
Toseland 10.233
ProMoST 10.511
Dawson 10.511
Bjellqvist 10.35
Wikipedia 10.804
Rodwell 10.482
Grimsley 10.613
Solomon 10.599
Lehninger 10.54
Nozaki 10.321
DTASelect 10.321
Thurlkill 10.35
EMBOSS 10.687
Sillero 10.438
Patrickios 9.999
IPC_peptide 10.599
IPC2_peptide 9.706
IPC2.peptide.svr19 8.412
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
99
1
100
47463
76
2123
474.6
53.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.6 ± 0.206
2.448 ± 0.122
6.816 ± 0.166
5.396 ± 0.178
3.984 ± 0.127
6.228 ± 0.263
2.476 ± 0.086
4.633 ± 0.212
3.5 ± 0.196
8.887 ± 0.216
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.404 ± 0.103
3.455 ± 0.156
5.103 ± 0.211
2.724 ± 0.137
8.198 ± 0.276
8.076 ± 0.202
6.066 ± 0.219
7.665 ± 0.178
0.95 ± 0.069
3.392 ± 0.103
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here