Arachidicoccus rhizosphaerae
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3837 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H3W9Q1|A0A1H3W9Q1_9BACT 1 4-alpha-glucan branching enzyme GlgB OS=Arachidicoccus rhizosphaerae OX=551991 GN=glgB PE=3 SV=1
MM1 pKa = 7.9 RR2 pKa = 11.84 FGNINLTSQITYY14 pKa = 9.98 YY15 pKa = 10.81 FSDD18 pKa = 3.75 LQSVKK23 pKa = 10.72 DD24 pKa = 3.66 QLMDD28 pKa = 3.46 NSDD31 pKa = 4.21 YY32 pKa = 11.3 IGDD35 pKa = 3.85 PP36 pKa = 3.43
Molecular weight: 4.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.935
IPC_protein 3.745
Toseland 3.528
ProMoST 4.012
Dawson 3.795
Bjellqvist 3.973
Wikipedia 3.859
Rodwell 3.592
Grimsley 3.465
Solomon 3.745
Lehninger 3.694
Nozaki 3.961
DTASelect 4.253
Thurlkill 3.668
EMBOSS 3.846
Sillero 3.884
Patrickios 1.952
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.802
Protein with the highest isoelectric point:
>tr|A0A1H4ATF4|A0A1H4ATF4_9BACT Cytidine deaminase OS=Arachidicoccus rhizosphaerae OX=551991 GN=SAMN05192529_11662 PE=4 SV=1
MM1 pKa = 7.27 IRR3 pKa = 11.84 FFIRR7 pKa = 11.84 IVFTIPVIIKK17 pKa = 8.58 FFTIIMRR24 pKa = 11.84 SISIKK29 pKa = 10.1 NSIWCIHH36 pKa = 5.84 ILLRR40 pKa = 11.84 ILNQVGANTDD50 pKa = 3.59 CRR52 pKa = 11.84 STTLPPNILTWYY64 pKa = 8.75 KK65 pKa = 9.19 TICEE69 pKa = 4.24 HH70 pKa = 6.75 KK71 pKa = 10.86 KK72 pKa = 8.3 MAIYY76 pKa = 10.01 FNRR79 pKa = 11.84 IYY81 pKa = 10.64 RR82 pKa = 11.84 IVSRR86 pKa = 11.84 VAIWSKK92 pKa = 10.54 RR93 pKa = 11.84 IINFRR98 pKa = 11.84 ILIFYY103 pKa = 9.94 LPSSNLCIIKK113 pKa = 8.53 TCAVIILVKK122 pKa = 10.57 SQVFLEE128 pKa = 4.72 FFAVVSILVMQWVCFLRR145 pKa = 5.4
Molecular weight: 17.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.765
IPC_protein 10.394
Toseland 10.643
ProMoST 10.467
Dawson 10.76
Bjellqvist 10.452
Wikipedia 10.935
Rodwell 11.096
Grimsley 10.804
Solomon 10.833
Lehninger 10.804
Nozaki 10.672
DTASelect 10.438
Thurlkill 10.643
EMBOSS 11.038
Sillero 10.687
Patrickios 10.833
IPC_peptide 10.833
IPC2_peptide 9.721
IPC2.peptide.svr19 8.482
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3837
0
3837
1398744
28
3013
364.5
40.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.579 ± 0.039
0.815 ± 0.011
5.543 ± 0.025
5.187 ± 0.039
4.677 ± 0.027
7.178 ± 0.036
2.004 ± 0.016
6.951 ± 0.035
6.783 ± 0.034
9.631 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.351 ± 0.02
5.204 ± 0.036
3.981 ± 0.019
4.302 ± 0.025
3.987 ± 0.024
6.646 ± 0.034
5.65 ± 0.033
5.921 ± 0.027
1.339 ± 0.016
4.27 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here