Saccharopolyspora coralli

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Saccharopolyspora

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4296 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q3QGE1|A0A5Q3QGE1_9PSEU Helix-turn-helix domain-containing protein OS=Saccharopolyspora coralli OX=2665642 GN=GIY23_14755 PE=4 SV=1
MM1 pKa = 7.53RR2 pKa = 11.84TRR4 pKa = 11.84TLLAGAATVVVATGMAVPAAVVAAPSPDD32 pKa = 3.48YY33 pKa = 10.69SAPAAMTHH41 pKa = 5.22LQEE44 pKa = 4.69LDD46 pKa = 3.59RR47 pKa = 11.84IAAEE51 pKa = 4.05NGGNRR56 pKa = 11.84AVGTPGYY63 pKa = 9.44EE64 pKa = 3.59AALDD68 pKa = 3.97YY69 pKa = 10.95VQQQLDD75 pKa = 3.27QAGFVTTRR83 pKa = 11.84QEE85 pKa = 3.71FTYY88 pKa = 10.84GGTTTWNLLAEE99 pKa = 4.55TEE101 pKa = 4.53GGNADD106 pKa = 3.43NTVVVGAHH114 pKa = 6.52LDD116 pKa = 3.88GVEE119 pKa = 4.23SGHH122 pKa = 7.03GINDD126 pKa = 3.59NGSGSAAVLNSALQYY141 pKa = 10.6AEE143 pKa = 4.82SGAEE147 pKa = 3.84PANKK151 pKa = 9.73LRR153 pKa = 11.84FAFWGAEE160 pKa = 3.92EE161 pKa = 4.22VGLVGSTHH169 pKa = 6.03YY170 pKa = 11.31VDD172 pKa = 4.02TLSQQEE178 pKa = 3.82RR179 pKa = 11.84DD180 pKa = 3.76QIAMYY185 pKa = 10.65LNYY188 pKa = 11.24DD189 pKa = 3.5MVGSNNAGYY198 pKa = 7.86FTYY201 pKa = 10.71DD202 pKa = 3.2GDD204 pKa = 4.48DD205 pKa = 3.48SDD207 pKa = 5.57GVGAGPGPDD216 pKa = 3.12GSAEE220 pKa = 3.88IEE222 pKa = 4.16QALNDD227 pKa = 4.19ALSGAGVEE235 pKa = 4.3PDD237 pKa = 3.12GTDD240 pKa = 3.37FDD242 pKa = 4.22GRR244 pKa = 11.84SDD246 pKa = 3.81YY247 pKa = 11.58GPFIEE252 pKa = 5.33VGIPSGGIFTGAEE265 pKa = 3.33GRR267 pKa = 11.84MTQEE271 pKa = 5.17QAQKK275 pKa = 8.44WNGRR279 pKa = 11.84AGEE282 pKa = 4.51PYY284 pKa = 10.08DD285 pKa = 3.68ACYY288 pKa = 10.04HH289 pKa = 5.06QACDD293 pKa = 5.9DD294 pKa = 4.2LDD296 pKa = 3.94NLNNEE301 pKa = 4.21AFEE304 pKa = 4.77KK305 pKa = 10.13NSQVISSTVADD316 pKa = 5.42FAAQLPIPQSS326 pKa = 3.23

Molecular weight: 33.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q3QC39|A0A5Q3QC39_9PSEU RDD family protein OS=Saccharopolyspora coralli OX=2665642 GN=GIY23_07460 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84KK12 pKa = 8.98RR13 pKa = 11.84AKK15 pKa = 8.46THH17 pKa = 5.11GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84GRR40 pKa = 11.84NKK42 pKa = 9.17LTAA45 pKa = 4.19

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4296

0

4296

1342545

33

2035

312.5

33.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.422 ± 0.044

0.833 ± 0.01

6.341 ± 0.037

6.125 ± 0.035

2.835 ± 0.022

9.144 ± 0.036

2.429 ± 0.023

3.205 ± 0.026

1.876 ± 0.026

10.103 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.863 ± 0.014

1.902 ± 0.019

5.716 ± 0.033

3.089 ± 0.023

7.892 ± 0.042

5.564 ± 0.024

6.104 ± 0.027

9.187 ± 0.042

1.489 ± 0.017

1.88 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski