Polaromonas vacuolata

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Polaromonas

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3376 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H2HD28|A0A6H2HD28_9BURK Translocation and assembly module subunit TamB OS=Polaromonas vacuolata OX=37448 GN=tamB PE=4 SV=1
MM1 pKa = 7.18SAAAEE6 pKa = 4.1LVTTEE11 pKa = 4.21MPSPFVFTDD20 pKa = 3.71SAASKK25 pKa = 9.65VAEE28 pKa = 4.86LIAEE32 pKa = 4.35EE33 pKa = 4.62GNPDD37 pKa = 2.99LKK39 pKa = 11.13LRR41 pKa = 11.84VFVQGGGCSGFQYY54 pKa = 10.94GFTFDD59 pKa = 4.81EE60 pKa = 4.64ITNEE64 pKa = 4.77DD65 pKa = 4.2DD66 pKa = 3.25TTMTKK71 pKa = 10.66NGVSLLIDD79 pKa = 3.54AMSYY83 pKa = 10.32QYY85 pKa = 11.5LVGAEE90 pKa = 4.01IDD92 pKa = 3.89YY93 pKa = 11.28KK94 pKa = 11.39DD95 pKa = 4.47DD96 pKa = 4.08LEE98 pKa = 4.56GAQFVIKK105 pKa = 10.53NPNATSTCGCGSSFSAA121 pKa = 4.79

Molecular weight: 12.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H2H4U4|A0A6H2H4U4_9BURK Uncharacterized protein OS=Polaromonas vacuolata OX=37448 GN=HC248_00085 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.31QPSKK9 pKa = 8.92VKK11 pKa = 10.35RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 6.27GFLTRR21 pKa = 11.84MKK23 pKa = 9.36TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.8GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3376

0

3376

1095282

29

7532

324.4

35.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.659 ± 0.055

0.963 ± 0.016

4.999 ± 0.032

4.997 ± 0.042

3.751 ± 0.025

7.553 ± 0.046

2.025 ± 0.02

5.134 ± 0.031

4.24 ± 0.043

11.16 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.657 ± 0.024

3.406 ± 0.033

4.648 ± 0.03

4.37 ± 0.034

5.564 ± 0.034

6.672 ± 0.044

5.387 ± 0.042

7.195 ± 0.044

1.351 ± 0.02

2.27 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski