Shigella phage SP18

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Gaprivervirus; Shigella virus SP18

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 284 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3SFF1|E3SFF1_9CAUD Uncharacterized protein a-gt.5 OS=Shigella phage SP18 OX=645664 GN=a-gt.5 PE=4 SV=1
MM1 pKa = 7.8RR2 pKa = 11.84LYY4 pKa = 10.93DD5 pKa = 3.82FNEE8 pKa = 4.25AGLEE12 pKa = 4.15HH13 pKa = 7.53IRR15 pKa = 11.84EE16 pKa = 4.28CDD18 pKa = 3.83VVDD21 pKa = 6.32DD22 pKa = 4.97DD23 pKa = 5.14NQPNTIKK30 pKa = 10.67YY31 pKa = 8.09WFEE34 pKa = 4.98AITDD38 pKa = 3.95RR39 pKa = 11.84EE40 pKa = 4.22TGQLIKK46 pKa = 10.89VYY48 pKa = 10.64VVTDD52 pKa = 3.6WAVEE56 pKa = 4.0FSFNVMEE63 pKa = 5.94DD64 pKa = 3.71DD65 pKa = 3.78TPEE68 pKa = 4.19TLIHH72 pKa = 5.85MAEE75 pKa = 4.12SCIKK79 pKa = 10.31DD80 pKa = 3.28AYY82 pKa = 10.2YY83 pKa = 10.93DD84 pKa = 3.64EE85 pKa = 5.92DD86 pKa = 3.8FF87 pKa = 4.89

Molecular weight: 10.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3SF30|E3SF30_9CAUD Uncharacterized protein gp31.2 OS=Shigella phage SP18 OX=645664 GN=gp31.2 PE=4 SV=1
MM1 pKa = 7.53AKK3 pKa = 10.16KK4 pKa = 9.46EE5 pKa = 4.17VKK7 pKa = 10.53VVDD10 pKa = 4.48GSSKK14 pKa = 9.21RR15 pKa = 11.84AGYY18 pKa = 9.82KK19 pKa = 9.3RR20 pKa = 11.84ASNKK24 pKa = 10.09RR25 pKa = 11.84INQVTDD31 pKa = 3.05KK32 pKa = 10.71LQARR36 pKa = 11.84ARR38 pKa = 11.84AVLRR42 pKa = 11.84ADD44 pKa = 3.85AACFGKK50 pKa = 9.94PKK52 pKa = 10.62AA53 pKa = 4.22

Molecular weight: 5.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

284

0

284

53101

37

1299

187.0

21.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.132 ± 0.172

1.088 ± 0.075

6.346 ± 0.109

7.299 ± 0.178

4.397 ± 0.11

6.22 ± 0.213

1.812 ± 0.093

6.81 ± 0.123

7.535 ± 0.195

7.422 ± 0.128

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.917 ± 0.111

5.399 ± 0.155

3.618 ± 0.096

3.719 ± 0.121

4.222 ± 0.105

5.851 ± 0.138

5.943 ± 0.212

6.606 ± 0.131

1.411 ± 0.056

4.252 ± 0.121
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski