Amino acid dipepetide frequency for Shigella phage SP18

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.8AlaAla: 5.8 ± 0.395
0.508AlaCys: 0.508 ± 0.103
4.369AlaAsp: 4.369 ± 0.289
5.273AlaGlu: 5.273 ± 0.371
2.731AlaPhe: 2.731 ± 0.245
4.953AlaGly: 4.953 ± 0.36
1.13AlaHis: 1.13 ± 0.176
4.991AlaIle: 4.991 ± 0.362
5.348AlaLys: 5.348 ± 0.351
5.725AlaLeu: 5.725 ± 0.355
1.94AlaMet: 1.94 ± 0.171
3.315AlaAsn: 3.315 ± 0.28
2.806AlaPro: 2.806 ± 0.281
2.712AlaGln: 2.712 ± 0.226
3.183AlaArg: 3.183 ± 0.245
4.294AlaSer: 4.294 ± 0.317
4.313AlaThr: 4.313 ± 0.513
4.181AlaVal: 4.181 ± 0.255
1.055AlaTrp: 1.055 ± 0.135
2.693AlaTyr: 2.693 ± 0.228
0.0AlaXaa: 0.0 ± 0.0
Cys
0.979CysAla: 0.979 ± 0.13
0.188CysCys: 0.188 ± 0.064
0.772CysAsp: 0.772 ± 0.111
0.998CysGlu: 0.998 ± 0.179
0.414CysPhe: 0.414 ± 0.082
0.753CysGly: 0.753 ± 0.132
0.282CysHis: 0.282 ± 0.076
0.603CysIle: 0.603 ± 0.114
0.81CysLys: 0.81 ± 0.132
0.697CysLeu: 0.697 ± 0.102
0.358CysMet: 0.358 ± 0.088
0.414CysAsn: 0.414 ± 0.086
0.433CysPro: 0.433 ± 0.093
0.395CysGln: 0.395 ± 0.081
0.49CysArg: 0.49 ± 0.1
0.621CysSer: 0.621 ± 0.117
0.433CysThr: 0.433 ± 0.088
0.678CysVal: 0.678 ± 0.118
0.132CysTrp: 0.132 ± 0.058
0.414CysTyr: 0.414 ± 0.079
0.0CysXaa: 0.0 ± 0.0
Asp
4.482AspAla: 4.482 ± 0.306
0.734AspCys: 0.734 ± 0.115
4.03AspAsp: 4.03 ± 0.28
4.689AspGlu: 4.689 ± 0.297
2.938AspPhe: 2.938 ± 0.249
4.765AspGly: 4.765 ± 0.273
0.885AspHis: 0.885 ± 0.126
4.67AspIle: 4.67 ± 0.333
4.652AspLys: 4.652 ± 0.274
4.859AspLeu: 4.859 ± 0.356
1.996AspMet: 1.996 ± 0.212
3.145AspAsn: 3.145 ± 0.254
2.015AspPro: 2.015 ± 0.243
2.034AspGln: 2.034 ± 0.193
1.902AspArg: 1.902 ± 0.208
3.616AspSer: 3.616 ± 0.235
3.126AspThr: 3.126 ± 0.24
4.313AspVal: 4.313 ± 0.27
1.318AspTrp: 1.318 ± 0.151
3.296AspTyr: 3.296 ± 0.255
0.0AspXaa: 0.0 ± 0.0
Glu
5.876GluAla: 5.876 ± 0.4
0.942GluCys: 0.942 ± 0.15
4.124GluAsp: 4.124 ± 0.303
5.217GluGlu: 5.217 ± 0.434
3.936GluPhe: 3.936 ± 0.277
3.729GluGly: 3.729 ± 0.219
1.375GluHis: 1.375 ± 0.153
5.405GluIle: 5.405 ± 0.28
4.331GluLys: 4.331 ± 0.307
6.667GluLeu: 6.667 ± 0.383
2.222GluMet: 2.222 ± 0.185
3.616GluAsn: 3.616 ± 0.267
1.996GluPro: 1.996 ± 0.193
2.693GluGln: 2.693 ± 0.261
2.9GluArg: 2.9 ± 0.234
3.879GluSer: 3.879 ± 0.302
4.388GluThr: 4.388 ± 0.272
5.047GluVal: 5.047 ± 0.266
1.111GluTrp: 1.111 ± 0.117
3.54GluTyr: 3.54 ± 0.304
0.0GluXaa: 0.0 ± 0.0
Phe
2.919PheAla: 2.919 ± 0.205
0.471PheCys: 0.471 ± 0.093
3.352PheAsp: 3.352 ± 0.275
3.484PheGlu: 3.484 ± 0.284
1.507PhePhe: 1.507 ± 0.185
3.107PheGly: 3.107 ± 0.246
0.678PheHis: 0.678 ± 0.12
2.825PheIle: 2.825 ± 0.186
3.879PheLys: 3.879 ± 0.279
2.542PheLeu: 2.542 ± 0.247
1.337PheMet: 1.337 ± 0.167
2.863PheAsn: 2.863 ± 0.26
1.281PhePro: 1.281 ± 0.16
1.45PheGln: 1.45 ± 0.177
1.921PheArg: 1.921 ± 0.156
2.448PheSer: 2.448 ± 0.2
2.467PheThr: 2.467 ± 0.259
2.976PheVal: 2.976 ± 0.263
0.697PheTrp: 0.697 ± 0.101
1.77PheTyr: 1.77 ± 0.189
0.0PheXaa: 0.0 ± 0.0
Gly
3.691GlyAla: 3.691 ± 0.265
0.697GlyCys: 0.697 ± 0.12
4.087GlyAsp: 4.087 ± 0.305
3.842GlyGlu: 3.842 ± 0.265
2.938GlyPhe: 2.938 ± 0.266
3.597GlyGly: 3.597 ± 0.261
0.923GlyHis: 0.923 ± 0.13
4.369GlyIle: 4.369 ± 0.269
4.256GlyLys: 4.256 ± 0.306
5.179GlyLeu: 5.179 ± 0.263
2.034GlyMet: 2.034 ± 0.223
3.352GlyAsn: 3.352 ± 0.354
2.072GlyPro: 2.072 ± 0.202
2.429GlyGln: 2.429 ± 0.226
2.674GlyArg: 2.674 ± 0.243
3.955GlySer: 3.955 ± 0.285
4.162GlyThr: 4.162 ± 0.429
4.087GlyVal: 4.087 ± 0.307
1.149GlyTrp: 1.149 ± 0.175
2.712GlyTyr: 2.712 ± 0.19
0.0GlyXaa: 0.0 ± 0.0
His
0.942HisAla: 0.942 ± 0.145
0.151HisCys: 0.151 ± 0.057
0.942HisAsp: 0.942 ± 0.138
1.299HisGlu: 1.299 ± 0.137
0.904HisPhe: 0.904 ± 0.143
1.073HisGly: 1.073 ± 0.157
0.395HisHis: 0.395 ± 0.088
1.281HisIle: 1.281 ± 0.17
1.337HisLys: 1.337 ± 0.143
1.318HisLeu: 1.318 ± 0.168
0.527HisMet: 0.527 ± 0.083
0.791HisAsn: 0.791 ± 0.14
1.224HisPro: 1.224 ± 0.145
0.734HisGln: 0.734 ± 0.112
0.885HisArg: 0.885 ± 0.118
0.96HisSer: 0.96 ± 0.147
0.998HisThr: 0.998 ± 0.162
1.281HisVal: 1.281 ± 0.14
0.282HisTrp: 0.282 ± 0.067
0.791HisTyr: 0.791 ± 0.125
0.0HisXaa: 0.0 ± 0.0
Ile
5.009IleAla: 5.009 ± 0.397
0.678IleCys: 0.678 ± 0.107
4.859IleAsp: 4.859 ± 0.323
5.348IleGlu: 5.348 ± 0.304
2.448IlePhe: 2.448 ± 0.213
3.427IleGly: 3.427 ± 0.248
1.205IleHis: 1.205 ± 0.153
4.143IleIle: 4.143 ± 0.269
6.328IleLys: 6.328 ± 0.41
3.559IleLeu: 3.559 ± 0.247
2.222IleMet: 2.222 ± 0.181
4.03IleAsn: 4.03 ± 0.237
2.768IlePro: 2.768 ± 0.23
2.731IleGln: 2.731 ± 0.211
3.333IleArg: 3.333 ± 0.249
4.068IleSer: 4.068 ± 0.309
4.52IleThr: 4.52 ± 0.303
4.407IleVal: 4.407 ± 0.274
0.527IleTrp: 0.527 ± 0.095
2.486IleTyr: 2.486 ± 0.221
0.0IleXaa: 0.0 ± 0.0
Lys
5.819LysAla: 5.819 ± 0.341
0.716LysCys: 0.716 ± 0.129
4.294LysAsp: 4.294 ± 0.248
6.026LysGlu: 6.026 ± 0.418
3.333LysPhe: 3.333 ± 0.249
4.52LysGly: 4.52 ± 0.324
1.714LysHis: 1.714 ± 0.194
4.783LysIle: 4.783 ± 0.266
4.633LysLys: 4.633 ± 0.337
6.234LysLeu: 6.234 ± 0.354
2.467LysMet: 2.467 ± 0.217
3.898LysAsn: 3.898 ± 0.313
2.863LysPro: 2.863 ± 0.225
2.806LysGln: 2.806 ± 0.234
3.427LysArg: 3.427 ± 0.296
4.181LysSer: 4.181 ± 0.35
4.087LysThr: 4.087 ± 0.28
5.367LysVal: 5.367 ± 0.352
1.036LysTrp: 1.036 ± 0.139
3.145LysTyr: 3.145 ± 0.25
0.0LysXaa: 0.0 ± 0.0
Leu
5.235LeuAla: 5.235 ± 0.297
0.753LeuCys: 0.753 ± 0.136
5.085LeuAsp: 5.085 ± 0.322
5.33LeuGlu: 5.33 ± 0.315
3.051LeuPhe: 3.051 ± 0.272
4.105LeuGly: 4.105 ± 0.275
1.224LeuHis: 1.224 ± 0.121
4.802LeuIle: 4.802 ± 0.277
6.422LeuLys: 6.422 ± 0.36
5.085LeuLeu: 5.085 ± 0.376
2.335LeuMet: 2.335 ± 0.211
4.896LeuAsn: 4.896 ± 0.272
3.051LeuPro: 3.051 ± 0.244
2.806LeuGln: 2.806 ± 0.26
2.957LeuArg: 2.957 ± 0.194
4.426LeuSer: 4.426 ± 0.305
4.35LeuThr: 4.35 ± 0.296
4.501LeuVal: 4.501 ± 0.32
0.678LeuTrp: 0.678 ± 0.124
3.126LeuTyr: 3.126 ± 0.262
0.0LeuXaa: 0.0 ± 0.0
Met
2.072MetAla: 2.072 ± 0.197
0.358MetCys: 0.358 ± 0.076
1.544MetAsp: 1.544 ± 0.191
1.94MetGlu: 1.94 ± 0.186
1.544MetPhe: 1.544 ± 0.17
1.62MetGly: 1.62 ± 0.172
0.49MetHis: 0.49 ± 0.089
2.109MetIle: 2.109 ± 0.19
2.58MetLys: 2.58 ± 0.236
2.072MetLeu: 2.072 ± 0.189
0.772MetMet: 0.772 ± 0.127
1.94MetAsn: 1.94 ± 0.146
0.791MetPro: 0.791 ± 0.103
1.017MetGln: 1.017 ± 0.144
1.149MetArg: 1.149 ± 0.145
2.222MetSer: 2.222 ± 0.198
1.921MetThr: 1.921 ± 0.191
1.695MetVal: 1.695 ± 0.204
0.264MetTrp: 0.264 ± 0.073
1.073MetTyr: 1.073 ± 0.158
0.0MetXaa: 0.0 ± 0.0
Asn
3.785AsnAla: 3.785 ± 0.296
0.659AsnCys: 0.659 ± 0.113
3.164AsnAsp: 3.164 ± 0.265
3.898AsnGlu: 3.898 ± 0.284
2.241AsnPhe: 2.241 ± 0.183
4.237AsnGly: 4.237 ± 0.321
1.073AsnHis: 1.073 ± 0.163
3.522AsnIle: 3.522 ± 0.243
4.162AsnLys: 4.162 ± 0.277
4.143AsnLeu: 4.143 ± 0.293
1.601AsnMet: 1.601 ± 0.208
2.844AsnAsn: 2.844 ± 0.272
2.166AsnPro: 2.166 ± 0.223
1.921AsnGln: 1.921 ± 0.204
2.335AsnArg: 2.335 ± 0.194
2.693AsnSer: 2.693 ± 0.216
3.202AsnThr: 3.202 ± 0.288
3.371AsnVal: 3.371 ± 0.284
0.584AsnTrp: 0.584 ± 0.108
2.392AsnTyr: 2.392 ± 0.213
0.0AsnXaa: 0.0 ± 0.0
Pro
2.524ProAla: 2.524 ± 0.244
0.471ProCys: 0.471 ± 0.108
2.712ProAsp: 2.712 ± 0.236
3.333ProGlu: 3.333 ± 0.274
1.544ProPhe: 1.544 ± 0.14
2.599ProGly: 2.599 ± 0.234
0.734ProHis: 0.734 ± 0.134
2.053ProIle: 2.053 ± 0.218
2.26ProLys: 2.26 ± 0.207
2.373ProLeu: 2.373 ± 0.182
0.659ProMet: 0.659 ± 0.091
1.714ProAsn: 1.714 ± 0.184
0.998ProPro: 0.998 ± 0.197
0.942ProGln: 0.942 ± 0.13
1.243ProArg: 1.243 ± 0.149
2.203ProSer: 2.203 ± 0.231
2.637ProThr: 2.637 ± 0.226
3.089ProVal: 3.089 ± 0.252
0.64ProTrp: 0.64 ± 0.12
1.45ProTyr: 1.45 ± 0.184
0.0ProXaa: 0.0 ± 0.0
Gln
2.863GlnAla: 2.863 ± 0.23
0.226GlnCys: 0.226 ± 0.065
1.977GlnAsp: 1.977 ± 0.225
2.618GlnGlu: 2.618 ± 0.265
1.733GlnPhe: 1.733 ± 0.195
2.166GlnGly: 2.166 ± 0.189
0.716GlnHis: 0.716 ± 0.127
2.844GlnIle: 2.844 ± 0.236
2.448GlnLys: 2.448 ± 0.202
3.145GlnLeu: 3.145 ± 0.247
1.036GlnMet: 1.036 ± 0.131
1.62GlnAsn: 1.62 ± 0.154
1.205GlnPro: 1.205 ± 0.148
1.243GlnGln: 1.243 ± 0.173
1.883GlnArg: 1.883 ± 0.163
1.846GlnSer: 1.846 ± 0.168
2.75GlnThr: 2.75 ± 0.258
2.241GlnVal: 2.241 ± 0.211
0.734GlnTrp: 0.734 ± 0.124
1.902GlnTyr: 1.902 ± 0.203
0.0GlnXaa: 0.0 ± 0.0
Arg
3.089ArgAla: 3.089 ± 0.312
0.433ArgCys: 0.433 ± 0.085
2.674ArgAsp: 2.674 ± 0.228
3.126ArgGlu: 3.126 ± 0.253
1.883ArgPhe: 1.883 ± 0.173
2.411ArgGly: 2.411 ± 0.218
0.942ArgHis: 0.942 ± 0.152
3.352ArgIle: 3.352 ± 0.286
3.145ArgLys: 3.145 ± 0.278
3.352ArgLeu: 3.352 ± 0.256
1.356ArgMet: 1.356 ± 0.151
2.053ArgAsn: 2.053 ± 0.183
1.224ArgPro: 1.224 ± 0.137
1.733ArgGln: 1.733 ± 0.195
2.241ArgArg: 2.241 ± 0.22
2.222ArgSer: 2.222 ± 0.208
2.241ArgThr: 2.241 ± 0.186
2.655ArgVal: 2.655 ± 0.241
0.565ArgTrp: 0.565 ± 0.099
1.525ArgTyr: 1.525 ± 0.194
0.0ArgXaa: 0.0 ± 0.0
Ser
3.578SerAla: 3.578 ± 0.293
0.772SerCys: 0.772 ± 0.138
3.823SerAsp: 3.823 ± 0.3
3.992SerGlu: 3.992 ± 0.251
2.674SerPhe: 2.674 ± 0.23
4.124SerGly: 4.124 ± 0.265
1.186SerHis: 1.186 ± 0.21
4.143SerIle: 4.143 ± 0.269
4.313SerLys: 4.313 ± 0.318
4.407SerLeu: 4.407 ± 0.288
1.544SerMet: 1.544 ± 0.169
3.032SerAsn: 3.032 ± 0.264
2.015SerPro: 2.015 ± 0.223
2.298SerGln: 2.298 ± 0.198
2.693SerArg: 2.693 ± 0.222
3.597SerSer: 3.597 ± 0.321
3.183SerThr: 3.183 ± 0.263
3.804SerVal: 3.804 ± 0.252
0.621SerTrp: 0.621 ± 0.116
2.712SerTyr: 2.712 ± 0.187
0.0SerXaa: 0.0 ± 0.0
Thr
4.35ThrAla: 4.35 ± 0.322
0.471ThrCys: 0.471 ± 0.094
3.446ThrAsp: 3.446 ± 0.287
4.256ThrGlu: 4.256 ± 0.281
2.844ThrPhe: 2.844 ± 0.261
4.539ThrGly: 4.539 ± 0.384
0.885ThrHis: 0.885 ± 0.126
4.124ThrIle: 4.124 ± 0.302
4.482ThrLys: 4.482 ± 0.304
4.407ThrLeu: 4.407 ± 0.345
1.356ThrMet: 1.356 ± 0.136
2.976ThrAsn: 2.976 ± 0.223
2.712ThrPro: 2.712 ± 0.262
2.072ThrGln: 2.072 ± 0.249
2.392ThrArg: 2.392 ± 0.327
3.729ThrSer: 3.729 ± 0.327
3.39ThrThr: 3.39 ± 0.334
4.162ThrVal: 4.162 ± 0.292
0.81ThrTrp: 0.81 ± 0.127
2.034ThrTyr: 2.034 ± 0.165
0.0ThrXaa: 0.0 ± 0.0
Val
4.407ValAla: 4.407 ± 0.254
1.017ValCys: 1.017 ± 0.121
4.463ValAsp: 4.463 ± 0.313
4.878ValGlu: 4.878 ± 0.347
2.712ValPhe: 2.712 ± 0.224
3.371ValGly: 3.371 ± 0.232
1.168ValHis: 1.168 ± 0.159
4.049ValIle: 4.049 ± 0.278
5.518ValLys: 5.518 ± 0.34
4.52ValLeu: 4.52 ± 0.281
1.846ValMet: 1.846 ± 0.203
3.898ValAsn: 3.898 ± 0.269
2.618ValPro: 2.618 ± 0.256
2.806ValGln: 2.806 ± 0.26
2.58ValArg: 2.58 ± 0.22
4.068ValSer: 4.068 ± 0.27
3.898ValThr: 3.898 ± 0.278
4.426ValVal: 4.426 ± 0.352
1.017ValTrp: 1.017 ± 0.169
2.806ValTyr: 2.806 ± 0.21
0.0ValXaa: 0.0 ± 0.0
Trp
0.923TrpAla: 0.923 ± 0.123
0.151TrpCys: 0.151 ± 0.053
0.923TrpAsp: 0.923 ± 0.127
0.923TrpGlu: 0.923 ± 0.128
0.659TrpPhe: 0.659 ± 0.107
0.377TrpGly: 0.377 ± 0.09
0.282TrpHis: 0.282 ± 0.077
0.942TrpIle: 0.942 ± 0.129
1.318TrpLys: 1.318 ± 0.147
1.149TrpLeu: 1.149 ± 0.169
0.358TrpMet: 0.358 ± 0.076
0.885TrpAsn: 0.885 ± 0.13
0.414TrpPro: 0.414 ± 0.081
0.603TrpGln: 0.603 ± 0.102
0.395TrpArg: 0.395 ± 0.085
0.81TrpSer: 0.81 ± 0.126
0.998TrpThr: 0.998 ± 0.117
0.942TrpVal: 0.942 ± 0.136
0.264TrpTrp: 0.264 ± 0.069
0.791TrpTyr: 0.791 ± 0.123
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.976TyrAla: 2.976 ± 0.264
0.49TyrCys: 0.49 ± 0.102
2.825TyrAsp: 2.825 ± 0.226
2.524TyrGlu: 2.524 ± 0.243
1.94TyrPhe: 1.94 ± 0.189
2.731TyrGly: 2.731 ± 0.244
0.829TyrHis: 0.829 ± 0.123
3.089TyrIle: 3.089 ± 0.262
3.126TyrLys: 3.126 ± 0.262
2.787TyrLeu: 2.787 ± 0.242
1.186TyrMet: 1.186 ± 0.165
2.712TyrAsn: 2.712 ± 0.267
1.525TyrPro: 1.525 ± 0.173
1.77TyrGln: 1.77 ± 0.21
1.638TyrArg: 1.638 ± 0.179
2.674TyrSer: 2.674 ± 0.228
2.373TyrThr: 2.373 ± 0.237
2.844TyrVal: 2.844 ± 0.222
0.621TyrTrp: 0.621 ± 0.111
1.864TyrTyr: 1.864 ± 0.195
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 284 proteins (53101 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski