Amino acid dipepetide frequency for Helicosporidium sp. ATCC 50920

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.667AlaAla: 18.667 ± 0.164	1.949AlaCys: 1.949 ± 0.038	5.85AlaAsp: 5.85 ± 0.061	8.206AlaGlu: 8.206 ± 0.094	3.869AlaPhe: 3.869 ± 0.051	10.152AlaGly: 10.152 ± 0.097	2.638AlaHis: 2.638 ± 0.043	2.994AlaIle: 2.994 ± 0.044	3.86AlaLys: 3.86 ± 0.066	14.473AlaLeu: 14.473 ± 0.121	2.5AlaMet: 2.5 ± 0.037	2.151AlaAsn: 2.151 ± 0.038	7.586AlaPro: 7.586 ± 0.098	4.901AlaGln: 4.901 ± 0.059	10.106AlaArg: 10.106 ± 0.106	9.713AlaSer: 9.713 ± 0.093	5.12AlaThr: 5.12 ± 0.061	8.577AlaVal: 8.577 ± 0.083	1.848AlaTrp: 1.848 ± 0.035	2.376AlaTyr: 2.376 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	1.873CysAla: 1.873 ± 0.035	0.336CysCys: 0.336 ± 0.013	0.713CysAsp: 0.713 ± 0.021	0.835CysGlu: 0.835 ± 0.022	0.549CysPhe: 0.549 ± 0.019	1.28CysGly: 1.28 ± 0.031	0.344CysHis: 0.344 ± 0.017	0.506CysIle: 0.506 ± 0.016	0.463CysLys: 0.463 ± 0.017	1.581CysLeu: 1.581 ± 0.035	0.329CysMet: 0.329 ± 0.013	0.359CysAsn: 0.359 ± 0.017	0.908CysPro: 0.908 ± 0.029	0.499CysGln: 0.499 ± 0.02	1.095CysArg: 1.095 ± 0.027	1.131CysSer: 1.131 ± 0.028	0.705CysThr: 0.705 ± 0.023	1.28CysVal: 1.28 ± 0.026	0.355CysTrp: 0.355 ± 0.023	0.334CysTyr: 0.334 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	7.528AspAla: 7.528 ± 0.078	0.776AspCys: 0.776 ± 0.021	2.933AspAsp: 2.933 ± 0.053	3.962AspGlu: 3.962 ± 0.056	1.674AspPhe: 1.674 ± 0.029	4.34AspGly: 4.34 ± 0.063	0.861AspHis: 0.861 ± 0.022	1.588AspIle: 1.588 ± 0.036	1.666AspLys: 1.666 ± 0.038	5.199AspLeu: 5.199 ± 0.061	1.034AspMet: 1.034 ± 0.027	0.992AspAsn: 0.992 ± 0.024	3.22AspPro: 3.22 ± 0.041	1.425AspGln: 1.425 ± 0.029	3.325AspArg: 3.325 ± 0.047	3.191AspSer: 3.191 ± 0.051	1.763AspThr: 1.763 ± 0.03	4.387AspVal: 4.387 ± 0.059	0.942AspTrp: 0.942 ± 0.025	1.227AspTyr: 1.227 ± 0.027	0.0AspXaa: 0.0 ± 0.0
Glu	9.526GluAla: 9.526 ± 0.091	0.779GluCys: 0.779 ± 0.023	4.072GluAsp: 4.072 ± 0.068	6.806GluGlu: 6.806 ± 0.124	1.65GluPhe: 1.65 ± 0.035	5.293GluGly: 5.293 ± 0.071	1.421GluHis: 1.421 ± 0.032	1.936GluIle: 1.936 ± 0.038	2.301GluLys: 2.301 ± 0.051	6.211GluLeu: 6.211 ± 0.068	1.28GluMet: 1.28 ± 0.028	1.334GluAsn: 1.334 ± 0.027	3.04GluPro: 3.04 ± 0.047	2.451GluGln: 2.451 ± 0.042	5.394GluArg: 5.394 ± 0.074	3.949GluSer: 3.949 ± 0.057	2.741GluThr: 2.741 ± 0.041	4.007GluVal: 4.007 ± 0.057	0.811GluTrp: 0.811 ± 0.022	1.264GluTyr: 1.264 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	3.575PheAla: 3.575 ± 0.051	0.629PheCys: 0.629 ± 0.02	1.867PheAsp: 1.867 ± 0.034	2.068PheGlu: 2.068 ± 0.033	1.32PhePhe: 1.32 ± 0.033	2.678PheGly: 2.678 ± 0.04	0.688PheHis: 0.688 ± 0.021	0.835PheIle: 0.835 ± 0.023	1.119PheLys: 1.119 ± 0.026	2.967PheLeu: 2.967 ± 0.05	0.678PheMet: 0.678 ± 0.019	0.855PheAsn: 0.855 ± 0.024	1.597PhePro: 1.597 ± 0.035	1.127PheGln: 1.127 ± 0.03	1.937PheArg: 1.937 ± 0.038	2.253PheSer: 2.253 ± 0.036	1.289PheThr: 1.289 ± 0.029	2.718PheVal: 2.718 ± 0.047	0.484PheTrp: 0.484 ± 0.017	0.881PheTyr: 0.881 ± 0.024	0.0PheXaa: 0.0 ± 0.0
Gly	10.33GlyAla: 10.33 ± 0.11	1.171GlyCys: 1.171 ± 0.03	4.162GlyAsp: 4.162 ± 0.053	5.425GlyGlu: 5.425 ± 0.07	2.545GlyPhe: 2.545 ± 0.04	8.627GlyGly: 8.627 ± 0.113	1.759GlyHis: 1.759 ± 0.038	2.397GlyIle: 2.397 ± 0.042	2.883GlyLys: 2.883 ± 0.048	7.466GlyLeu: 7.466 ± 0.071	1.787GlyMet: 1.787 ± 0.032	1.726GlyAsn: 1.726 ± 0.045	4.058GlyPro: 4.058 ± 0.056	2.92GlyGln: 2.92 ± 0.053	6.661GlyArg: 6.661 ± 0.11	6.432GlySer: 6.432 ± 0.073	3.667GlyThr: 3.667 ± 0.044	6.069GlyVal: 6.069 ± 0.064	1.173GlyTrp: 1.173 ± 0.026	1.691GlyTyr: 1.691 ± 0.042	0.0GlyXaa: 0.0 ± 0.0
His	2.953HisAla: 2.953 ± 0.049	0.407HisCys: 0.407 ± 0.017	1.031HisAsp: 1.031 ± 0.027	1.15HisGlu: 1.15 ± 0.025	0.741HisPhe: 0.741 ± 0.025	1.879HisGly: 1.879 ± 0.037	0.556HisHis: 0.556 ± 0.02	0.658HisIle: 0.658 ± 0.023	0.648HisLys: 0.648 ± 0.023	2.202HisLeu: 2.202 ± 0.043	0.459HisMet: 0.459 ± 0.018	0.493HisAsn: 0.493 ± 0.014	1.662HisPro: 1.662 ± 0.032	0.674HisGln: 0.674 ± 0.02	1.731HisArg: 1.731 ± 0.038	1.38HisSer: 1.38 ± 0.029	0.917HisThr: 0.917 ± 0.023	1.838HisVal: 1.838 ± 0.035	0.378HisTrp: 0.378 ± 0.015	0.545HisTyr: 0.545 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	3.013IleAla: 3.013 ± 0.05	0.488IleCys: 0.488 ± 0.019	1.546IleAsp: 1.546 ± 0.032	1.592IleGlu: 1.592 ± 0.032	1.152IlePhe: 1.152 ± 0.028	1.862IleGly: 1.862 ± 0.039	0.623IleHis: 0.623 ± 0.02	0.946IleIle: 0.946 ± 0.025	1.277IleLys: 1.277 ± 0.031	2.822IleLeu: 2.822 ± 0.043	0.674IleMet: 0.674 ± 0.023	0.818IleAsn: 0.818 ± 0.024	1.603IlePro: 1.603 ± 0.029	1.074IleGln: 1.074 ± 0.025	1.847IleArg: 1.847 ± 0.035	1.928IleSer: 1.928 ± 0.034	1.311IleThr: 1.311 ± 0.03	2.314IleVal: 2.314 ± 0.048	0.407IleTrp: 0.407 ± 0.015	0.721IleTyr: 0.721 ± 0.021	0.0IleXaa: 0.0 ± 0.0
Lys	3.973LysAla: 3.973 ± 0.06	0.356LysCys: 0.356 ± 0.014	1.791LysAsp: 1.791 ± 0.037	2.4LysGlu: 2.4 ± 0.054	0.894LysPhe: 0.894 ± 0.024	2.619LysGly: 2.619 ± 0.046	0.78LysHis: 0.78 ± 0.025	1.203LysIle: 1.203 ± 0.03	2.111LysLys: 2.111 ± 0.065	3.21LysLeu: 3.21 ± 0.052	0.749LysMet: 0.749 ± 0.022	0.956LysAsn: 0.956 ± 0.028	1.81LysPro: 1.81 ± 0.036	1.173LysGln: 1.173 ± 0.029	2.672LysArg: 2.672 ± 0.046	2.117LysSer: 2.117 ± 0.041	1.732LysThr: 1.732 ± 0.036	2.23LysVal: 2.23 ± 0.045	0.354LysTrp: 0.354 ± 0.015	0.817LysTyr: 0.817 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	12.55LeuAla: 12.55 ± 0.113	1.721LeuCys: 1.721 ± 0.032	5.727LeuAsp: 5.727 ± 0.058	6.719LeuGlu: 6.719 ± 0.079	3.208LeuPhe: 3.208 ± 0.051	7.991LeuGly: 7.991 ± 0.071	2.452LeuHis: 2.452 ± 0.039	2.357LeuIle: 2.357 ± 0.046	3.398LeuLys: 3.398 ± 0.047	10.55LeuLeu: 10.55 ± 0.098	1.793LeuMet: 1.793 ± 0.035	2.182LeuAsn: 2.182 ± 0.037	6.359LeuPro: 6.359 ± 0.071	4.085LeuGln: 4.085 ± 0.058	8.454LeuArg: 8.454 ± 0.088	7.462LeuSer: 7.462 ± 0.071	4.234LeuThr: 4.234 ± 0.057	7.266LeuVal: 7.266 ± 0.073	1.377LeuTrp: 1.377 ± 0.033	2.096LeuTyr: 2.096 ± 0.036	0.0LeuXaa: 0.0 ± 0.0
Met	2.425MetAla: 2.425 ± 0.034	0.273MetCys: 0.273 ± 0.011	1.195MetAsp: 1.195 ± 0.029	1.332MetGlu: 1.332 ± 0.027	0.573MetPhe: 0.573 ± 0.018	1.657MetGly: 1.657 ± 0.032	0.532MetHis: 0.532 ± 0.02	0.536MetIle: 0.536 ± 0.021	0.588MetLys: 0.588 ± 0.021	2.17MetLeu: 2.17 ± 0.036	0.417MetMet: 0.417 ± 0.017	0.483MetAsn: 0.483 ± 0.017	1.053MetPro: 1.053 ± 0.028	0.869MetGln: 0.869 ± 0.024	1.497MetArg: 1.497 ± 0.026	1.336MetSer: 1.336 ± 0.029	0.943MetThr: 0.943 ± 0.027	1.359MetVal: 1.359 ± 0.032	0.217MetTrp: 0.217 ± 0.012	0.465MetTyr: 0.465 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.673AsnAla: 2.673 ± 0.038	0.342AsnCys: 0.342 ± 0.016	1.002AsnAsp: 1.002 ± 0.024	1.118AsnGlu: 1.118 ± 0.027	0.81AsnPhe: 0.81 ± 0.024	1.709AsnGly: 1.709 ± 0.038	0.41AsnHis: 0.41 ± 0.015	0.829AsnIle: 0.829 ± 0.025	0.822AsnLys: 0.822 ± 0.022	2.146AsnLeu: 2.146 ± 0.032	0.5AsnMet: 0.5 ± 0.016	0.64AsnAsn: 0.64 ± 0.022	1.444AsnPro: 1.444 ± 0.032	0.657AsnGln: 0.657 ± 0.019	1.23AsnArg: 1.23 ± 0.029	1.366AsnSer: 1.366 ± 0.03	0.991AsnThr: 0.991 ± 0.027	1.859AsnVal: 1.859 ± 0.037	0.357AsnTrp: 0.357 ± 0.015	0.604AsnTyr: 0.604 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	7.563ProAla: 7.563 ± 0.097	0.809ProCys: 0.809 ± 0.026	3.019ProAsp: 3.019 ± 0.053	4.024ProGlu: 4.024 ± 0.049	1.717ProPhe: 1.717 ± 0.038	5.411ProGly: 5.411 ± 0.061	1.333ProHis: 1.333 ± 0.032	1.381ProIle: 1.381 ± 0.029	1.72ProLys: 1.72 ± 0.038	5.529ProLeu: 5.529 ± 0.062	0.966ProMet: 0.966 ± 0.025	1.172ProAsn: 1.172 ± 0.027	5.445ProPro: 5.445 ± 0.086	1.915ProGln: 1.915 ± 0.036	4.532ProArg: 4.532 ± 0.071	5.218ProSer: 5.218 ± 0.069	2.603ProThr: 2.603 ± 0.04	3.609ProVal: 3.609 ± 0.053	0.821ProTrp: 0.821 ± 0.024	1.185ProTyr: 1.185 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	4.842GlnAla: 4.842 ± 0.062	0.478GlnCys: 0.478 ± 0.018	1.642GlnAsp: 1.642 ± 0.032	2.273GlnGlu: 2.273 ± 0.047	1.003GlnPhe: 1.003 ± 0.022	3.192GlnGly: 3.192 ± 0.05	0.911GlnHis: 0.911 ± 0.024	1.081GlnIle: 1.081 ± 0.022	1.132GlnLys: 1.132 ± 0.028	3.631GlnLeu: 3.631 ± 0.048	0.685GlnMet: 0.685 ± 0.02	0.762GlnAsn: 0.762 ± 0.02	2.023GlnPro: 2.023 ± 0.042	1.534GlnGln: 1.534 ± 0.04	3.255GlnArg: 3.255 ± 0.047	2.307GlnSer: 2.307 ± 0.044	1.679GlnThr: 1.679 ± 0.031	2.32GlnVal: 2.32 ± 0.041	0.465GlnTrp: 0.465 ± 0.017	0.761GlnTyr: 0.761 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	10.294ArgAla: 10.294 ± 0.111	1.19ArgCys: 1.19 ± 0.028	3.815ArgAsp: 3.815 ± 0.052	5.046ArgGlu: 5.046 ± 0.065	2.289ArgPhe: 2.289 ± 0.036	6.853ArgGly: 6.853 ± 0.111	1.904ArgHis: 1.904 ± 0.034	2.01ArgIle: 2.01 ± 0.034	2.528ArgLys: 2.528 ± 0.045	7.961ArgLeu: 7.961 ± 0.078	1.535ArgMet: 1.535 ± 0.031	1.415ArgAsn: 1.415 ± 0.033	4.306ArgPro: 4.306 ± 0.062	2.788ArgGln: 2.788 ± 0.042	7.724ArgArg: 7.724 ± 0.126	4.864ArgSer: 4.864 ± 0.065	2.884ArgThr: 2.884 ± 0.043	5.467ArgVal: 5.467 ± 0.058	1.142ArgTrp: 1.142 ± 0.027	1.47ArgTyr: 1.47 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	8.611SerAla: 8.611 ± 0.094	1.083SerCys: 1.083 ± 0.031	3.455SerAsp: 3.455 ± 0.053	4.052SerGlu: 4.052 ± 0.057	2.321SerPhe: 2.321 ± 0.037	5.838SerGly: 5.838 ± 0.063	1.469SerHis: 1.469 ± 0.03	2.082SerIle: 2.082 ± 0.036	2.499SerLys: 2.499 ± 0.041	7.4SerLeu: 7.4 ± 0.062	1.444SerMet: 1.444 ± 0.034	1.612SerAsn: 1.612 ± 0.041	4.876SerPro: 4.876 ± 0.076	2.472SerGln: 2.472 ± 0.04	5.1SerArg: 5.1 ± 0.058	6.514SerSer: 6.514 ± 0.094	3.408SerThr: 3.408 ± 0.05	4.71SerVal: 4.71 ± 0.06	1.082SerTrp: 1.082 ± 0.028	1.462SerTyr: 1.462 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	4.649ThrAla: 4.649 ± 0.055	0.764ThrCys: 0.764 ± 0.023	1.903ThrAsp: 1.903 ± 0.036	2.194ThrGlu: 2.194 ± 0.038	1.538ThrPhe: 1.538 ± 0.029	3.446ThrGly: 3.446 ± 0.054	0.977ThrHis: 0.977 ± 0.025	1.525ThrIle: 1.525 ± 0.034	1.363ThrLys: 1.363 ± 0.033	5.093ThrLeu: 5.093 ± 0.062	0.894ThrMet: 0.894 ± 0.026	0.996ThrAsn: 0.996 ± 0.023	3.083ThrPro: 3.083 ± 0.044	1.461ThrGln: 1.461 ± 0.027	2.888ThrArg: 2.888 ± 0.044	3.151ThrSer: 3.151 ± 0.047	2.153ThrThr: 2.153 ± 0.041	3.27ThrVal: 3.27 ± 0.049	0.662ThrTrp: 0.662 ± 0.018	1.155ThrTyr: 1.155 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	8.504ValAla: 8.504 ± 0.075	1.24ValCys: 1.24 ± 0.026	4.117ValAsp: 4.117 ± 0.059	4.81ValGlu: 4.81 ± 0.054	2.405ValPhe: 2.405 ± 0.038	5.187ValGly: 5.187 ± 0.067	1.793ValHis: 1.793 ± 0.035	2.007ValIle: 2.007 ± 0.039	2.295ValLys: 2.295 ± 0.045	7.905ValLeu: 7.905 ± 0.08	1.394ValMet: 1.394 ± 0.03	1.487ValAsn: 1.487 ± 0.031	4.237ValPro: 4.237 ± 0.057	2.767ValGln: 2.767 ± 0.042	5.317ValArg: 5.317 ± 0.055	4.717ValSer: 4.717 ± 0.056	3.094ValThr: 3.094 ± 0.042	5.926ValVal: 5.926 ± 0.076	1.085ValTrp: 1.085 ± 0.026	1.736ValTyr: 1.736 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	1.561TrpAla: 1.561 ± 0.036	0.23TrpCys: 0.23 ± 0.012	0.864TrpAsp: 0.864 ± 0.026	0.974TrpGlu: 0.974 ± 0.026	0.421TrpPhe: 0.421 ± 0.016	1.213TrpGly: 1.213 ± 0.035	0.386TrpHis: 0.386 ± 0.014	0.453TrpIle: 0.453 ± 0.017	0.532TrpLys: 0.532 ± 0.02	1.342TrpLeu: 1.342 ± 0.033	0.34TrpMet: 0.34 ± 0.014	0.418TrpAsn: 0.418 ± 0.016	0.641TrpPro: 0.641 ± 0.022	0.52TrpGln: 0.52 ± 0.016	1.241TrpArg: 1.241 ± 0.031	1.017TrpSer: 1.017 ± 0.025	0.859TrpThr: 0.859 ± 0.025	0.971TrpVal: 0.971 ± 0.028	0.24TrpTrp: 0.24 ± 0.013	0.326TrpTyr: 0.326 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.426TyrAla: 2.426 ± 0.04	0.454TyrCys: 0.454 ± 0.014	1.329TyrAsp: 1.329 ± 0.03	1.278TyrGlu: 1.278 ± 0.032	0.888TyrPhe: 0.888 ± 0.024	1.72TyrGly: 1.72 ± 0.039	0.467TyrHis: 0.467 ± 0.017	0.696TyrIle: 0.696 ± 0.022	0.741TyrLys: 0.741 ± 0.023	2.124TyrLeu: 2.124 ± 0.042	0.463TyrMet: 0.463 ± 0.014	0.66TyrAsn: 0.66 ± 0.021	1.021TyrPro: 1.021 ± 0.03	0.662TyrGln: 0.662 ± 0.021	1.478TyrArg: 1.478 ± 0.036	1.441TyrSer: 1.441 ± 0.031	1.07TyrThr: 1.07 ± 0.033	1.845TyrVal: 1.845 ± 0.038	0.352TyrTrp: 0.352 ± 0.014	0.718TyrTyr: 0.718 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6029 proteins (1647305 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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