Synechococcus phage S-CBP42
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A096VKW4|A0A096VKW4_9CAUD Uncharacterized protein OS=Synechococcus phage S-CBP42 OX=461711 GN=S-CBP42_0054 PE=4 SV=1
MM1 pKa = 7.33 TYY3 pKa = 10.31 EE4 pKa = 4.15 YY5 pKa = 8.94 DD6 pKa = 3.37 TTTALSNEE14 pKa = 3.69 VLEE17 pKa = 4.4 YY18 pKa = 10.53 FRR20 pKa = 11.84 QQEE23 pKa = 4.21 EE24 pKa = 4.26 EE25 pKa = 3.98 QLLKK29 pKa = 10.58 QEE31 pKa = 4.93 QIDD34 pKa = 4.17 DD35 pKa = 4.04 LDD37 pKa = 4.36 DD38 pKa = 3.7 EE39 pKa = 4.47 KK40 pKa = 11.06 FAYY43 pKa = 9.3 FVAYY47 pKa = 10.36 GDD49 pKa = 3.79 FDD51 pKa = 3.82 YY52 pKa = 11.5 QRR54 pKa = 11.84 TFDD57 pKa = 3.68 LL58 pKa = 4.95
Molecular weight: 7.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.732
IPC_protein 3.643
Toseland 3.452
ProMoST 3.821
Dawson 3.643
Bjellqvist 3.808
Wikipedia 3.579
Rodwell 3.49
Grimsley 3.376
Solomon 3.605
Lehninger 3.567
Nozaki 3.77
DTASelect 3.935
Thurlkill 3.516
EMBOSS 3.592
Sillero 3.77
Patrickios 0.693
IPC_peptide 3.605
IPC2_peptide 3.745
IPC2.peptide.svr19 3.724
Protein with the highest isoelectric point:
>tr|A0A096VKU7|A0A096VKU7_9CAUD Uncharacterized protein OS=Synechococcus phage S-CBP42 OX=461711 GN=S-CBP42_0027 PE=4 SV=1
MM1 pKa = 7.67 HH2 pKa = 6.82 NTQFSAAVTAARR14 pKa = 11.84 LAAGQPVSTAALAHH28 pKa = 5.97 LAKK31 pKa = 10.61 VEE33 pKa = 3.98 AQIKK37 pKa = 8.77 QVQAARR43 pKa = 3.69
Molecular weight: 4.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.235
IPC2_protein 9.736
IPC_protein 10.54
Toseland 11.228
ProMoST 11.813
Dawson 11.257
Bjellqvist 10.994
Wikipedia 11.506
Rodwell 11.506
Grimsley 11.272
Solomon 11.52
Lehninger 11.477
Nozaki 11.199
DTASelect 11.008
Thurlkill 11.199
EMBOSS 11.652
Sillero 11.199
Patrickios 11.374
IPC_peptide 11.52
IPC2_peptide 9.897
IPC2.peptide.svr19 9.127
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51
0
51
10189
43
1610
199.8
22.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.609 ± 0.748
0.844 ± 0.22
6.281 ± 0.263
6.095 ± 0.369
3.229 ± 0.155
7.646 ± 0.447
1.59 ± 0.291
5.094 ± 0.206
5.604 ± 0.371
8.401 ± 0.353
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.355 ± 0.209
4.623 ± 0.352
4.407 ± 0.298
4.593 ± 0.391
5.643 ± 0.356
5.791 ± 0.412
6.213 ± 0.468
6.379 ± 0.328
1.364 ± 0.169
3.239 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here