Amino acid dipepetide frequency for Synechococcus phage S-CBP42

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.938AlaAla: 13.938 ± 2.071
0.491AlaCys: 0.491 ± 0.353
6.478AlaAsp: 6.478 ± 0.782
6.282AlaGlu: 6.282 ± 0.832
2.454AlaPhe: 2.454 ± 0.55
8.736AlaGly: 8.736 ± 0.964
1.767AlaHis: 1.767 ± 0.441
4.81AlaIle: 4.81 ± 0.6
5.693AlaLys: 5.693 ± 1.04
9.521AlaLeu: 9.521 ± 0.884
2.65AlaMet: 2.65 ± 0.599
5.791AlaAsn: 5.791 ± 1.909
3.828AlaPro: 3.828 ± 0.821
5.3AlaGln: 5.3 ± 1.176
4.122AlaArg: 4.122 ± 0.677
7.263AlaSer: 7.263 ± 1.208
6.38AlaThr: 6.38 ± 1.425
6.576AlaVal: 6.576 ± 0.888
1.08AlaTrp: 1.08 ± 0.416
2.945AlaTyr: 2.945 ± 0.582
0.0AlaXaa: 0.0 ± 0.0
Cys
0.294CysAla: 0.294 ± 0.179
0.196CysCys: 0.196 ± 0.154
0.589CysAsp: 0.589 ± 0.259
0.294CysGlu: 0.294 ± 0.185
0.589CysPhe: 0.589 ± 0.245
0.491CysGly: 0.491 ± 0.247
0.0CysHis: 0.0 ± 0.0
0.785CysIle: 0.785 ± 0.364
0.687CysLys: 0.687 ± 0.301
0.687CysLeu: 0.687 ± 0.352
0.393CysMet: 0.393 ± 0.243
0.294CysAsn: 0.294 ± 0.17
0.589CysPro: 0.589 ± 0.316
0.393CysGln: 0.393 ± 0.201
0.785CysArg: 0.785 ± 0.361
0.393CysSer: 0.393 ± 0.248
0.393CysThr: 0.393 ± 0.197
0.491CysVal: 0.491 ± 0.228
0.098CysTrp: 0.098 ± 0.122
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
6.086AspAla: 6.086 ± 0.706
0.491AspCys: 0.491 ± 0.284
3.141AspAsp: 3.141 ± 0.732
4.711AspGlu: 4.711 ± 0.691
2.748AspPhe: 2.748 ± 0.568
6.184AspGly: 6.184 ± 1.381
0.785AspHis: 0.785 ± 0.298
3.043AspIle: 3.043 ± 0.577
2.748AspLys: 2.748 ± 0.575
6.086AspLeu: 6.086 ± 0.958
1.276AspMet: 1.276 ± 0.422
1.767AspAsn: 1.767 ± 0.353
3.435AspPro: 3.435 ± 0.434
2.748AspGln: 2.748 ± 0.599
4.024AspArg: 4.024 ± 0.584
3.043AspSer: 3.043 ± 0.647
3.926AspThr: 3.926 ± 0.764
3.435AspVal: 3.435 ± 0.463
1.178AspTrp: 1.178 ± 0.319
1.963AspTyr: 1.963 ± 0.429
0.0AspXaa: 0.0 ± 0.0
Glu
7.263GluAla: 7.263 ± 0.99
0.687GluCys: 0.687 ± 0.288
4.122GluAsp: 4.122 ± 0.784
4.908GluGlu: 4.908 ± 0.993
2.159GluPhe: 2.159 ± 0.425
3.534GluGly: 3.534 ± 0.6
0.785GluHis: 0.785 ± 0.235
4.515GluIle: 4.515 ± 0.754
1.669GluLys: 1.669 ± 0.409
5.497GluLeu: 5.497 ± 0.845
0.982GluMet: 0.982 ± 0.269
2.356GluAsn: 2.356 ± 0.409
1.374GluPro: 1.374 ± 0.333
2.945GluGln: 2.945 ± 0.677
3.043GluArg: 3.043 ± 0.461
3.141GluSer: 3.141 ± 0.652
3.73GluThr: 3.73 ± 0.791
5.202GluVal: 5.202 ± 0.645
0.883GluTrp: 0.883 ± 0.261
2.159GluTyr: 2.159 ± 0.417
0.0GluXaa: 0.0 ± 0.0
Phe
1.767PheAla: 1.767 ± 0.455
0.294PheCys: 0.294 ± 0.171
3.239PheAsp: 3.239 ± 0.782
1.57PheGlu: 1.57 ± 0.335
1.276PhePhe: 1.276 ± 0.287
2.258PheGly: 2.258 ± 0.591
0.883PheHis: 0.883 ± 0.281
1.178PheIle: 1.178 ± 0.355
1.963PheLys: 1.963 ± 0.42
2.258PheLeu: 2.258 ± 0.385
0.687PheMet: 0.687 ± 0.265
2.159PheAsn: 2.159 ± 0.561
1.276PhePro: 1.276 ± 0.289
1.669PheGln: 1.669 ± 0.333
1.767PheArg: 1.767 ± 0.322
1.767PheSer: 1.767 ± 0.368
2.748PheThr: 2.748 ± 0.645
2.65PheVal: 2.65 ± 0.55
0.098PheTrp: 0.098 ± 0.11
0.785PheTyr: 0.785 ± 0.266
0.0PheXaa: 0.0 ± 0.0
Gly
6.969GlyAla: 6.969 ± 1.072
0.491GlyCys: 0.491 ± 0.262
4.711GlyAsp: 4.711 ± 0.575
4.319GlyGlu: 4.319 ± 0.818
3.534GlyPhe: 3.534 ± 0.551
4.81GlyGly: 4.81 ± 1.023
1.57GlyHis: 1.57 ± 0.486
5.006GlyIle: 5.006 ± 0.892
5.595GlyLys: 5.595 ± 1.135
7.558GlyLeu: 7.558 ± 0.83
1.08GlyMet: 1.08 ± 0.29
3.337GlyAsn: 3.337 ± 0.588
3.337GlyPro: 3.337 ± 0.588
2.846GlyGln: 2.846 ± 0.461
4.711GlyArg: 4.711 ± 0.806
4.221GlySer: 4.221 ± 1.067
3.337GlyThr: 3.337 ± 0.603
4.613GlyVal: 4.613 ± 0.596
1.767GlyTrp: 1.767 ± 0.427
2.65GlyTyr: 2.65 ± 0.462
0.0GlyXaa: 0.0 ± 0.0
His
0.883HisAla: 0.883 ± 0.244
0.098HisCys: 0.098 ± 0.102
0.982HisAsp: 0.982 ± 0.41
1.178HisGlu: 1.178 ± 0.383
0.785HisPhe: 0.785 ± 0.312
0.687HisGly: 0.687 ± 0.271
0.687HisHis: 0.687 ± 0.348
0.687HisIle: 0.687 ± 0.254
0.982HisLys: 0.982 ± 0.33
1.57HisLeu: 1.57 ± 0.635
0.393HisMet: 0.393 ± 0.21
0.785HisAsn: 0.785 ± 0.258
0.982HisPro: 0.982 ± 0.312
0.687HisGln: 0.687 ± 0.232
0.785HisArg: 0.785 ± 0.342
1.276HisSer: 1.276 ± 0.548
0.687HisThr: 0.687 ± 0.227
1.08HisVal: 1.08 ± 0.361
0.098HisTrp: 0.098 ± 0.103
0.589HisTyr: 0.589 ± 0.226
0.0HisXaa: 0.0 ± 0.0
Ile
6.282IleAla: 6.282 ± 0.939
0.491IleCys: 0.491 ± 0.259
4.417IleAsp: 4.417 ± 0.696
3.435IleGlu: 3.435 ± 0.513
0.687IlePhe: 0.687 ± 0.248
2.945IleGly: 2.945 ± 0.54
0.491IleHis: 0.491 ± 0.223
1.865IleIle: 1.865 ± 0.477
2.65IleLys: 2.65 ± 0.456
4.024IleLeu: 4.024 ± 0.79
1.57IleMet: 1.57 ± 0.324
1.669IleAsn: 1.669 ± 0.448
2.846IlePro: 2.846 ± 0.405
2.356IleGln: 2.356 ± 0.667
3.141IleArg: 3.141 ± 0.613
2.945IleSer: 2.945 ± 0.476
3.73IleThr: 3.73 ± 0.857
3.239IleVal: 3.239 ± 0.504
0.393IleTrp: 0.393 ± 0.153
1.767IleTyr: 1.767 ± 0.463
0.0IleXaa: 0.0 ± 0.0
Lys
7.165LysAla: 7.165 ± 0.928
0.098LysCys: 0.098 ± 0.111
3.337LysAsp: 3.337 ± 0.714
3.632LysGlu: 3.632 ± 0.61
1.276LysPhe: 1.276 ± 0.398
3.141LysGly: 3.141 ± 0.545
0.589LysHis: 0.589 ± 0.263
2.552LysIle: 2.552 ± 0.757
3.73LysLys: 3.73 ± 0.855
4.81LysLeu: 4.81 ± 0.637
1.472LysMet: 1.472 ± 0.509
2.454LysAsn: 2.454 ± 0.589
2.846LysPro: 2.846 ± 0.553
3.337LysGln: 3.337 ± 0.607
2.945LysArg: 2.945 ± 0.604
3.043LysSer: 3.043 ± 0.697
3.141LysThr: 3.141 ± 0.687
3.73LysVal: 3.73 ± 0.599
1.08LysTrp: 1.08 ± 0.301
1.669LysTyr: 1.669 ± 0.479
0.0LysXaa: 0.0 ± 0.0
Leu
8.638LeuAla: 8.638 ± 1.039
1.08LeuCys: 1.08 ± 0.382
5.791LeuAsp: 5.791 ± 0.755
5.497LeuGlu: 5.497 ± 0.725
2.846LeuPhe: 2.846 ± 0.47
6.184LeuGly: 6.184 ± 0.947
1.767LeuHis: 1.767 ± 0.432
3.926LeuIle: 3.926 ± 0.611
5.889LeuLys: 5.889 ± 1.105
5.399LeuLeu: 5.399 ± 0.571
2.159LeuMet: 2.159 ± 0.48
3.926LeuAsn: 3.926 ± 0.576
3.435LeuPro: 3.435 ± 0.654
2.945LeuGln: 2.945 ± 0.407
6.184LeuArg: 6.184 ± 0.851
6.184LeuSer: 6.184 ± 0.751
4.515LeuThr: 4.515 ± 0.595
4.417LeuVal: 4.417 ± 0.741
0.883LeuTrp: 0.883 ± 0.27
2.356LeuTyr: 2.356 ± 0.491
0.0LeuXaa: 0.0 ± 0.0
Met
2.748MetAla: 2.748 ± 0.601
0.098MetCys: 0.098 ± 0.095
1.276MetAsp: 1.276 ± 0.344
1.767MetGlu: 1.767 ± 0.332
0.098MetPhe: 0.098 ± 0.103
2.159MetGly: 2.159 ± 0.449
0.098MetHis: 0.098 ± 0.098
1.374MetIle: 1.374 ± 0.346
1.669MetLys: 1.669 ± 0.451
1.767MetLeu: 1.767 ± 0.404
0.491MetMet: 0.491 ± 0.206
1.178MetAsn: 1.178 ± 0.275
0.687MetPro: 0.687 ± 0.255
1.472MetGln: 1.472 ± 0.514
0.982MetArg: 0.982 ± 0.26
1.472MetSer: 1.472 ± 0.37
2.061MetThr: 2.061 ± 0.61
1.472MetVal: 1.472 ± 0.481
0.196MetTrp: 0.196 ± 0.15
0.491MetTyr: 0.491 ± 0.294
0.0MetXaa: 0.0 ± 0.0
Asn
4.613AsnAla: 4.613 ± 1.054
0.196AsnCys: 0.196 ± 0.121
2.061AsnAsp: 2.061 ± 0.51
2.159AsnGlu: 2.159 ± 0.441
1.276AsnPhe: 1.276 ± 0.449
3.926AsnGly: 3.926 ± 0.708
0.393AsnHis: 0.393 ± 0.226
2.454AsnIle: 2.454 ± 0.455
1.963AsnLys: 1.963 ± 0.403
3.926AsnLeu: 3.926 ± 0.899
1.178AsnMet: 1.178 ± 0.363
2.945AsnAsn: 2.945 ± 0.859
2.748AsnPro: 2.748 ± 0.553
2.258AsnGln: 2.258 ± 0.606
2.454AsnArg: 2.454 ± 0.562
2.356AsnSer: 2.356 ± 0.503
3.926AsnThr: 3.926 ± 1.207
3.239AsnVal: 3.239 ± 0.53
0.589AsnTrp: 0.589 ± 0.259
1.57AsnTyr: 1.57 ± 0.394
0.0AsnXaa: 0.0 ± 0.0
Pro
3.534ProAla: 3.534 ± 0.626
0.491ProCys: 0.491 ± 0.251
2.846ProAsp: 2.846 ± 0.578
3.043ProGlu: 3.043 ± 0.634
1.865ProPhe: 1.865 ± 0.625
3.926ProGly: 3.926 ± 0.835
0.883ProHis: 0.883 ± 0.356
2.552ProIle: 2.552 ± 0.379
2.258ProLys: 2.258 ± 0.426
2.356ProLeu: 2.356 ± 0.433
1.08ProMet: 1.08 ± 0.267
2.552ProAsn: 2.552 ± 0.373
2.258ProPro: 2.258 ± 0.868
1.669ProGln: 1.669 ± 0.512
1.865ProArg: 1.865 ± 0.486
2.159ProSer: 2.159 ± 0.344
2.748ProThr: 2.748 ± 0.548
3.141ProVal: 3.141 ± 0.562
0.883ProTrp: 0.883 ± 0.456
1.963ProTyr: 1.963 ± 0.528
0.0ProXaa: 0.0 ± 0.0
Gln
5.889GlnAla: 5.889 ± 1.135
0.098GlnCys: 0.098 ± 0.133
1.472GlnAsp: 1.472 ± 0.382
2.945GlnGlu: 2.945 ± 0.569
1.963GlnPhe: 1.963 ± 0.584
4.221GlnGly: 4.221 ± 0.88
0.883GlnHis: 0.883 ± 0.388
2.356GlnIle: 2.356 ± 0.528
1.08GlnLys: 1.08 ± 0.407
5.202GlnLeu: 5.202 ± 1.046
0.883GlnMet: 0.883 ± 0.334
1.669GlnAsn: 1.669 ± 0.418
2.258GlnPro: 2.258 ± 0.607
3.926GlnGln: 3.926 ± 0.894
2.846GlnArg: 2.846 ± 0.543
1.472GlnSer: 1.472 ± 0.534
1.374GlnThr: 1.374 ± 0.464
3.337GlnVal: 3.337 ± 0.638
0.589GlnTrp: 0.589 ± 0.274
1.472GlnTyr: 1.472 ± 0.586
0.0GlnXaa: 0.0 ± 0.0
Arg
5.595ArgAla: 5.595 ± 0.846
0.196ArgCys: 0.196 ± 0.151
2.846ArgAsp: 2.846 ± 0.495
3.239ArgGlu: 3.239 ± 0.505
1.276ArgPhe: 1.276 ± 0.41
3.239ArgGly: 3.239 ± 0.677
0.982ArgHis: 0.982 ± 0.368
3.337ArgIle: 3.337 ± 0.526
5.202ArgLys: 5.202 ± 0.926
5.693ArgLeu: 5.693 ± 0.689
2.159ArgMet: 2.159 ± 0.476
2.258ArgAsn: 2.258 ± 0.451
2.159ArgPro: 2.159 ± 0.4
2.552ArgGln: 2.552 ± 0.486
3.435ArgArg: 3.435 ± 0.548
3.828ArgSer: 3.828 ± 0.598
3.043ArgThr: 3.043 ± 0.666
2.945ArgVal: 2.945 ± 0.433
0.785ArgTrp: 0.785 ± 0.319
1.669ArgTyr: 1.669 ± 0.366
0.0ArgXaa: 0.0 ± 0.0
Ser
5.595SerAla: 5.595 ± 0.818
0.589SerCys: 0.589 ± 0.28
2.552SerAsp: 2.552 ± 0.549
2.846SerGlu: 2.846 ± 0.688
1.963SerPhe: 1.963 ± 0.598
5.693SerGly: 5.693 ± 0.872
1.08SerHis: 1.08 ± 0.294
3.337SerIle: 3.337 ± 0.792
3.534SerLys: 3.534 ± 0.56
3.337SerLeu: 3.337 ± 0.514
0.982SerMet: 0.982 ± 0.356
3.239SerAsn: 3.239 ± 0.631
2.65SerPro: 2.65 ± 0.457
1.767SerGln: 1.767 ± 0.409
2.846SerArg: 2.846 ± 0.665
3.534SerSer: 3.534 ± 1.244
5.399SerThr: 5.399 ± 0.843
3.73SerVal: 3.73 ± 0.863
0.982SerTrp: 0.982 ± 0.264
2.258SerTyr: 2.258 ± 0.441
0.0SerXaa: 0.0 ± 0.0
Thr
7.067ThrAla: 7.067 ± 2.538
0.393ThrCys: 0.393 ± 0.202
4.122ThrAsp: 4.122 ± 0.75
3.141ThrGlu: 3.141 ± 0.52
1.865ThrPhe: 1.865 ± 0.392
7.46ThrGly: 7.46 ± 1.115
0.687ThrHis: 0.687 ± 0.208
2.159ThrIle: 2.159 ± 0.579
3.043ThrLys: 3.043 ± 0.524
4.711ThrLeu: 4.711 ± 0.911
1.178ThrMet: 1.178 ± 0.377
3.141ThrAsn: 3.141 ± 0.542
2.552ThrPro: 2.552 ± 0.531
1.865ThrGln: 1.865 ± 0.452
3.337ThrArg: 3.337 ± 0.447
2.846ThrSer: 2.846 ± 0.581
4.81ThrThr: 4.81 ± 1.17
4.319ThrVal: 4.319 ± 0.898
0.982ThrTrp: 0.982 ± 0.29
2.356ThrTyr: 2.356 ± 0.577
0.0ThrXaa: 0.0 ± 0.0
Val
7.656ValAla: 7.656 ± 0.885
1.08ValCys: 1.08 ± 0.408
5.006ValAsp: 5.006 ± 0.674
3.239ValGlu: 3.239 ± 0.527
2.748ValPhe: 2.748 ± 0.534
4.711ValGly: 4.711 ± 0.813
0.589ValHis: 0.589 ± 0.293
2.454ValIle: 2.454 ± 0.384
2.846ValLys: 2.846 ± 0.385
6.086ValLeu: 6.086 ± 0.847
1.767ValMet: 1.767 ± 0.395
3.043ValAsn: 3.043 ± 0.615
2.945ValPro: 2.945 ± 0.562
2.846ValGln: 2.846 ± 0.565
3.73ValArg: 3.73 ± 0.528
3.435ValSer: 3.435 ± 0.646
3.435ValThr: 3.435 ± 0.408
3.73ValVal: 3.73 ± 0.803
0.491ValTrp: 0.491 ± 0.228
1.963ValTyr: 1.963 ± 0.402
0.0ValXaa: 0.0 ± 0.0
Trp
1.767TrpAla: 1.767 ± 0.349
0.491TrpCys: 0.491 ± 0.282
1.374TrpAsp: 1.374 ± 0.291
0.687TrpGlu: 0.687 ± 0.253
0.196TrpPhe: 0.196 ± 0.158
0.589TrpGly: 0.589 ± 0.243
0.196TrpHis: 0.196 ± 0.133
0.785TrpIle: 0.785 ± 0.256
0.491TrpLys: 0.491 ± 0.227
1.178TrpLeu: 1.178 ± 0.359
0.393TrpMet: 0.393 ± 0.186
0.785TrpAsn: 0.785 ± 0.385
0.687TrpPro: 0.687 ± 0.286
0.687TrpGln: 0.687 ± 0.177
0.687TrpArg: 0.687 ± 0.292
0.491TrpSer: 0.491 ± 0.285
0.687TrpThr: 0.687 ± 0.282
0.687TrpVal: 0.687 ± 0.265
0.196TrpTrp: 0.196 ± 0.145
0.589TrpTyr: 0.589 ± 0.218
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.356TyrAla: 2.356 ± 0.488
0.393TyrCys: 0.393 ± 0.2
2.454TyrAsp: 2.454 ± 0.569
2.061TyrGlu: 2.061 ± 0.467
0.687TyrPhe: 0.687 ± 0.398
1.57TyrGly: 1.57 ± 0.42
0.785TyrHis: 0.785 ± 0.295
1.767TyrIle: 1.767 ± 0.434
2.356TyrLys: 2.356 ± 0.405
2.356TyrLeu: 2.356 ± 0.487
0.687TyrMet: 0.687 ± 0.252
0.883TyrAsn: 0.883 ± 0.234
1.178TyrPro: 1.178 ± 0.31
1.669TyrGln: 1.669 ± 0.539
2.748TyrArg: 2.748 ± 0.655
3.043TyrSer: 3.043 ± 0.518
2.061TyrThr: 2.061 ± 0.442
1.767TyrVal: 1.767 ± 0.48
0.393TyrTrp: 0.393 ± 0.228
1.178TyrTyr: 1.178 ± 0.361
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (10189 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski