Methyloceanibacter marginalis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2602 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E3WD35|A0A1E3WD35_9RHIZ ATP-grasp domain-containing protein OS=Methyloceanibacter marginalis OX=1774971 GN=AUC71_08615 PE=3 SV=1
MM1 pKa = 7.08STDD4 pKa = 4.02DD5 pKa = 4.85GLITEE10 pKa = 5.46DD11 pKa = 3.85GAEE14 pKa = 4.04GAYY17 pKa = 10.35LVASQLLWYY26 pKa = 10.11RR27 pKa = 11.84DD28 pKa = 3.76PQPTNDD34 pKa = 4.3AGISGYY40 pKa = 9.61IQFGWNDD47 pKa = 3.27SEE49 pKa = 4.44TQLFNYY55 pKa = 9.7YY56 pKa = 10.23FGAGFTGRR64 pKa = 11.84ALRR67 pKa = 11.84VV68 pKa = 3.25

Molecular weight: 7.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E3WCE5|A0A1E3WCE5_9RHIZ Transglycosylase OS=Methyloceanibacter marginalis OX=1774971 GN=AUC71_10960 PE=4 SV=1
MM1 pKa = 7.53WGLPGAAAGACLAFAASLAASWLVGRR27 pKa = 11.84RR28 pKa = 11.84LLDD31 pKa = 3.41IPHH34 pKa = 7.14AFGEE38 pKa = 4.52VARR41 pKa = 11.84ITVAVGVMLAALWVIQPPDD60 pKa = 3.59GVVGLVIEE68 pKa = 4.41IVTGGVAMSPLPWLSTYY85 pKa = 8.07WKK87 pKa = 9.76CVRR90 pKa = 11.84HH91 pKa = 5.81SLSISRR97 pKa = 11.84AWCKK101 pKa = 10.56SRR103 pKa = 11.84WVRR106 pKa = 11.84RR107 pKa = 11.84DD108 pKa = 3.24RR109 pKa = 11.84VDD111 pKa = 2.92QSSAVFGRR119 pKa = 11.84GHH121 pKa = 5.6QKK123 pKa = 10.64

Molecular weight: 13.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2602

0

2602

634150

29

1738

243.7

26.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.036 ± 0.064

0.956 ± 0.017

5.799 ± 0.044

6.148 ± 0.05

3.743 ± 0.032

8.425 ± 0.05

2.033 ± 0.027

4.924 ± 0.037

3.848 ± 0.048

10.062 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.37 ± 0.026

2.531 ± 0.03

5.38 ± 0.039

2.982 ± 0.027

6.991 ± 0.055

5.389 ± 0.037

5.239 ± 0.034

7.333 ± 0.036

1.412 ± 0.021

2.397 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski