Sporanaerobacter sp. PP17-6a

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Tissierellaceae; Sporanaerobacter; unclassified Sporanaerobacter

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3134 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G4FNG6|A0A1G4FNG6_9FIRM Ribonuclease H OS=Sporanaerobacter sp. PP17-6a OX=1891289 GN=rnhA PE=3 SV=1
MM1 pKa = 7.5GNIALQIEE9 pKa = 4.6RR10 pKa = 11.84TASGATVASTARR22 pKa = 11.84VVFNNLVYY30 pKa = 10.02STGDD34 pKa = 2.72IDD36 pKa = 4.49YY37 pKa = 10.47NPSTGIITLNSTGRR51 pKa = 11.84YY52 pKa = 8.2IINWWVAIQSTGSANGMIFSLLSSQSDD79 pKa = 4.31SITGSSPQIAGQVSGIGIIDD99 pKa = 3.79VVSAPVTLSLINSSTGTLTYY119 pKa = 10.62SVAMPVKK126 pKa = 10.2ASLMVFKK133 pKa = 10.64DD134 pKa = 3.94EE135 pKa = 5.11PSGNSNDD142 pKa = 3.68TTSLCFSYY150 pKa = 10.82AQLAHH155 pKa = 6.74VIEE158 pKa = 4.22QLITIYY164 pKa = 10.37SEE166 pKa = 4.16SVMSVFTTNVNTVTGTPYY184 pKa = 10.77QLYY187 pKa = 10.48ASPDD191 pKa = 3.53GDD193 pKa = 3.27NGAIFVLIDD202 pKa = 3.97SLGQFQAVPITLITAIYY219 pKa = 10.09VGDD222 pKa = 3.54NTVYY226 pKa = 10.85DD227 pKa = 4.15DD228 pKa = 4.81SITYY232 pKa = 7.5LTPPSPLPNGCDD244 pKa = 3.25TDD246 pKa = 4.97LIAAIRR252 pKa = 11.84DD253 pKa = 3.68YY254 pKa = 11.43LPISTEE260 pKa = 4.09VIMQMGATTQASGAIYY276 pKa = 10.06KK277 pKa = 10.08NEE279 pKa = 3.72YY280 pKa = 9.96GILVLSDD287 pKa = 3.66EE288 pKa = 4.86NGNTPIFIPVTKK300 pKa = 9.85IARR303 pKa = 11.84IVTAPPTPSKK313 pKa = 10.82KK314 pKa = 8.27PTPVSVINNPEE325 pKa = 4.17LNVPSNPDD333 pKa = 3.29NPNDD337 pKa = 3.53

Molecular weight: 35.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G4FGA3|A0A1G4FGA3_9FIRM Putative D D-dipeptide transport system permease protein DdpC OS=Sporanaerobacter sp. PP17-6a OX=1891289 GN=ddpC PE=3 SV=1
MM1 pKa = 7.64KK2 pKa = 10.42KK3 pKa = 10.18NNKK6 pKa = 9.6LILILTASILSVSMAGCGTKK26 pKa = 10.08TSQRR30 pKa = 11.84PNSRR34 pKa = 11.84VEE36 pKa = 3.89TRR38 pKa = 11.84IGNQAQRR45 pKa = 11.84RR46 pKa = 11.84TPNVMPRR53 pKa = 11.84SNVPRR58 pKa = 11.84LEE60 pKa = 4.32SNLNNNSGIDD70 pKa = 3.65NNGNTNLNTNNNGNMTQGSYY90 pKa = 9.0QRR92 pKa = 11.84AQNIARR98 pKa = 11.84RR99 pKa = 11.84VAALNDD105 pKa = 3.69VNSATAVITGNTALVGVDD123 pKa = 4.82LKK125 pKa = 11.19GTTNDD130 pKa = 3.48NLTTNIRR137 pKa = 11.84NKK139 pKa = 10.08VEE141 pKa = 4.23TAVKK145 pKa = 9.98EE146 pKa = 3.7ADD148 pKa = 3.6KK149 pKa = 11.37NITTVNVTTNPDD161 pKa = 3.27LYY163 pKa = 11.45SRR165 pKa = 11.84ISTIARR171 pKa = 11.84NISNGRR177 pKa = 11.84PMNDD181 pKa = 3.35FANDD185 pKa = 3.09IQDD188 pKa = 3.67ILRR191 pKa = 11.84KK192 pKa = 9.3MNLNRR197 pKa = 4.11

Molecular weight: 21.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3134

0

3134

970558

29

2835

309.7

35.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.314 ± 0.044

1.044 ± 0.017

5.693 ± 0.039

7.589 ± 0.054

4.376 ± 0.032

6.788 ± 0.042

1.304 ± 0.017

10.103 ± 0.048

9.044 ± 0.048

8.965 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.695 ± 0.019

5.904 ± 0.038

3.035 ± 0.025

2.32 ± 0.02

3.755 ± 0.029

6.154 ± 0.031

4.715 ± 0.029

6.357 ± 0.037

0.704 ± 0.013

4.14 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski