Amino acid dipepetide frequency for Sporanaerobacter sp. PP17-6a

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.688AlaAla: 3.688 ± 0.09	0.61AlaCys: 0.61 ± 0.031	3.042AlaAsp: 3.042 ± 0.068	3.732AlaGlu: 3.732 ± 0.071	2.311AlaPhe: 2.311 ± 0.054	3.901AlaGly: 3.901 ± 0.076	0.796AlaHis: 0.796 ± 0.033	4.914AlaIle: 4.914 ± 0.076	4.184AlaLys: 4.184 ± 0.073	5.41AlaLeu: 5.41 ± 0.081	1.55AlaMet: 1.55 ± 0.043	2.29AlaAsn: 2.29 ± 0.047	1.27AlaPro: 1.27 ± 0.05	1.522AlaGln: 1.522 ± 0.042	1.857AlaArg: 1.857 ± 0.047	2.894AlaSer: 2.894 ± 0.062	2.421AlaThr: 2.421 ± 0.063	4.447AlaVal: 4.447 ± 0.076	0.317AlaTrp: 0.317 ± 0.018	1.983AlaTyr: 1.983 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	0.578CysAla: 0.578 ± 0.025	0.161CysCys: 0.161 ± 0.015	0.547CysAsp: 0.547 ± 0.024	0.587CysGlu: 0.587 ± 0.025	0.414CysPhe: 0.414 ± 0.022	1.021CysGly: 1.021 ± 0.037	0.218CysHis: 0.218 ± 0.014	1.022CysIle: 1.022 ± 0.039	0.781CysLys: 0.781 ± 0.03	0.842CysLeu: 0.842 ± 0.03	0.275CysMet: 0.275 ± 0.018	0.613CysAsn: 0.613 ± 0.027	0.47CysPro: 0.47 ± 0.028	0.244CysGln: 0.244 ± 0.018	0.422CysArg: 0.422 ± 0.025	0.68CysSer: 0.68 ± 0.031	0.503CysThr: 0.503 ± 0.02	0.57CysVal: 0.57 ± 0.022	0.086CysTrp: 0.086 ± 0.01	0.408CysTyr: 0.408 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	2.534AspAla: 2.534 ± 0.055	0.551AspCys: 0.551 ± 0.027	2.829AspAsp: 2.829 ± 0.06	5.21AspGlu: 5.21 ± 0.081	2.776AspPhe: 2.776 ± 0.06	3.578AspGly: 3.578 ± 0.071	0.64AspHis: 0.64 ± 0.023	6.677AspIle: 6.677 ± 0.085	5.541AspLys: 5.541 ± 0.089	5.07AspLeu: 5.07 ± 0.073	1.745AspMet: 1.745 ± 0.042	3.151AspAsn: 3.151 ± 0.063	1.482AspPro: 1.482 ± 0.042	0.851AspGln: 0.851 ± 0.028	2.068AspArg: 2.068 ± 0.051	3.113AspSer: 3.113 ± 0.056	2.399AspThr: 2.399 ± 0.052	3.59AspVal: 3.59 ± 0.064	0.422AspTrp: 0.422 ± 0.021	2.708AspTyr: 2.708 ± 0.056	0.0AspXaa: 0.0 ± 0.0
Glu	3.648GluAla: 3.648 ± 0.064	0.553GluCys: 0.553 ± 0.021	4.589GluAsp: 4.589 ± 0.082	7.373GluGlu: 7.373 ± 0.114	2.779GluPhe: 2.779 ± 0.065	4.781GluGly: 4.781 ± 0.07	0.79GluHis: 0.79 ± 0.03	7.74GluIle: 7.74 ± 0.102	8.609GluLys: 8.609 ± 0.137	6.403GluLeu: 6.403 ± 0.084	2.066GluMet: 2.066 ± 0.049	5.374GluAsn: 5.374 ± 0.086	1.706GluPro: 1.706 ± 0.044	1.623GluGln: 1.623 ± 0.045	3.215GluArg: 3.215 ± 0.061	3.472GluSer: 3.472 ± 0.068	3.024GluThr: 3.024 ± 0.058	4.487GluVal: 4.487 ± 0.085	0.491GluTrp: 0.491 ± 0.024	3.165GluTyr: 3.165 ± 0.059	0.0GluXaa: 0.0 ± 0.0
Phe	2.241PheAla: 2.241 ± 0.044	0.486PheCys: 0.486 ± 0.022	2.55PheAsp: 2.55 ± 0.05	2.683PheGlu: 2.683 ± 0.056	2.038PhePhe: 2.038 ± 0.054	2.934PheGly: 2.934 ± 0.054	0.59PheHis: 0.59 ± 0.024	4.377PheIle: 4.377 ± 0.071	3.54PheLys: 3.54 ± 0.062	4.26PheLeu: 4.26 ± 0.083	1.157PheMet: 1.157 ± 0.032	2.651PheAsn: 2.651 ± 0.056	1.392PhePro: 1.392 ± 0.032	1.087PheGln: 1.087 ± 0.036	1.368PheArg: 1.368 ± 0.034	3.369PheSer: 3.369 ± 0.057	2.208PheThr: 2.208 ± 0.054	2.723PheVal: 2.723 ± 0.053	0.315PheTrp: 0.315 ± 0.018	1.787PheTyr: 1.787 ± 0.044	0.0PheXaa: 0.0 ± 0.0
Gly	3.852GlyAla: 3.852 ± 0.091	0.864GlyCys: 0.864 ± 0.031	3.441GlyAsp: 3.441 ± 0.06	4.812GlyGlu: 4.812 ± 0.078	3.089GlyPhe: 3.089 ± 0.059	4.571GlyGly: 4.571 ± 0.094	0.95GlyHis: 0.95 ± 0.034	7.661GlyIle: 7.661 ± 0.09	6.229GlyLys: 6.229 ± 0.086	5.728GlyLeu: 5.728 ± 0.093	1.922GlyMet: 1.922 ± 0.043	3.653GlyAsn: 3.653 ± 0.061	1.368GlyPro: 1.368 ± 0.041	1.576GlyGln: 1.576 ± 0.042	2.692GlyArg: 2.692 ± 0.06	3.72GlySer: 3.72 ± 0.059	3.612GlyThr: 3.612 ± 0.078	4.471GlyVal: 4.471 ± 0.079	0.533GlyTrp: 0.533 ± 0.023	3.141GlyTyr: 3.141 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	0.631HisAla: 0.631 ± 0.023	0.193HisCys: 0.193 ± 0.014	0.668HisAsp: 0.668 ± 0.028	0.822HisGlu: 0.822 ± 0.032	0.593HisPhe: 0.593 ± 0.024	1.043HisGly: 1.043 ± 0.031	0.256HisHis: 0.256 ± 0.018	1.352HisIle: 1.352 ± 0.042	0.92HisLys: 0.92 ± 0.033	1.146HisLeu: 1.146 ± 0.034	0.356HisMet: 0.356 ± 0.019	0.681HisAsn: 0.681 ± 0.027	0.636HisPro: 0.636 ± 0.025	0.355HisGln: 0.355 ± 0.02	0.569HisArg: 0.569 ± 0.019	0.802HisSer: 0.802 ± 0.032	0.615HisThr: 0.615 ± 0.027	0.788HisVal: 0.788 ± 0.029	0.106HisTrp: 0.106 ± 0.01	0.511HisTyr: 0.511 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.57IleAla: 5.57 ± 0.088	1.147IleCys: 1.147 ± 0.041	6.045IleAsp: 6.045 ± 0.071	7.031IleGlu: 7.031 ± 0.098	4.595IlePhe: 4.595 ± 0.093	6.609IleGly: 6.609 ± 0.099	1.279IleHis: 1.279 ± 0.042	9.771IleIle: 9.771 ± 0.136	8.813IleLys: 8.813 ± 0.102	9.454IleLeu: 9.454 ± 0.125	2.442IleMet: 2.442 ± 0.06	5.81IleAsn: 5.81 ± 0.089	3.798IlePro: 3.798 ± 0.067	2.263IleGln: 2.263 ± 0.051	3.579IleArg: 3.579 ± 0.059	7.348IleSer: 7.348 ± 0.108	4.796IleThr: 4.796 ± 0.073	6.387IleVal: 6.387 ± 0.079	0.624IleTrp: 0.624 ± 0.024	3.673IleTyr: 3.673 ± 0.067	0.0IleXaa: 0.0 ± 0.0
Lys	4.482LysAla: 4.482 ± 0.06	0.74LysCys: 0.74 ± 0.027	6.027LysAsp: 6.027 ± 0.091	9.041LysGlu: 9.041 ± 0.137	3.17LysPhe: 3.17 ± 0.051	5.817LysGly: 5.817 ± 0.083	0.932LysHis: 0.932 ± 0.033	9.004LysIle: 9.004 ± 0.105	8.59LysLys: 8.59 ± 0.115	7.088LysLeu: 7.088 ± 0.075	2.475LysMet: 2.475 ± 0.048	6.391LysAsn: 6.391 ± 0.105	2.118LysPro: 2.118 ± 0.051	1.78LysGln: 1.78 ± 0.042	3.572LysArg: 3.572 ± 0.069	4.975LysSer: 4.975 ± 0.075	4.02LysThr: 4.02 ± 0.061	5.43LysVal: 5.43 ± 0.082	0.636LysTrp: 0.636 ± 0.027	4.146LysTyr: 4.146 ± 0.076	0.0LysXaa: 0.0 ± 0.0
Leu	4.758LeuAla: 4.758 ± 0.093	0.989LeuCys: 0.989 ± 0.031	4.903LeuAsp: 4.903 ± 0.07	5.894LeuGlu: 5.894 ± 0.083	3.9LeuPhe: 3.9 ± 0.075	5.937LeuGly: 5.937 ± 0.089	1.007LeuHis: 1.007 ± 0.035	8.523LeuIle: 8.523 ± 0.112	8.989LeuLys: 8.989 ± 0.124	7.896LeuLeu: 7.896 ± 0.116	2.403LeuMet: 2.403 ± 0.054	5.968LeuAsn: 5.968 ± 0.079	3.062LeuPro: 3.062 ± 0.064	1.978LeuGln: 1.978 ± 0.044	3.412LeuArg: 3.412 ± 0.061	6.745LeuSer: 6.745 ± 0.096	4.408LeuThr: 4.408 ± 0.076	4.943LeuVal: 4.943 ± 0.082	0.626LeuTrp: 0.626 ± 0.031	3.314LeuTyr: 3.314 ± 0.062	0.0LeuXaa: 0.0 ± 0.0
Met	1.802MetAla: 1.802 ± 0.045	0.208MetCys: 0.208 ± 0.015	1.708MetAsp: 1.708 ± 0.041	2.139MetGlu: 2.139 ± 0.049	0.967MetPhe: 0.967 ± 0.034	2.071MetGly: 2.071 ± 0.051	0.295MetHis: 0.295 ± 0.018	2.372MetIle: 2.372 ± 0.053	2.773MetLys: 2.773 ± 0.057	2.18MetLeu: 2.18 ± 0.054	0.721MetMet: 0.721 ± 0.03	1.688MetAsn: 1.688 ± 0.046	0.838MetPro: 0.838 ± 0.03	0.495MetGln: 0.495 ± 0.023	0.99MetArg: 0.99 ± 0.031	1.666MetSer: 1.666 ± 0.04	1.322MetThr: 1.322 ± 0.033	1.699MetVal: 1.699 ± 0.041	0.151MetTrp: 0.151 ± 0.012	0.868MetTyr: 0.868 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.566AsnAla: 2.566 ± 0.049	0.669AsnCys: 0.669 ± 0.027	2.747AsnAsp: 2.747 ± 0.059	3.914AsnGlu: 3.914 ± 0.07	2.576AsnPhe: 2.576 ± 0.054	3.62AsnGly: 3.62 ± 0.068	0.749AsnHis: 0.749 ± 0.028	7.24AsnIle: 7.24 ± 0.102	5.36AsnLys: 5.36 ± 0.079	5.452AsnLeu: 5.452 ± 0.081	1.79AsnMet: 1.79 ± 0.04	3.645AsnAsn: 3.645 ± 0.082	2.313AsnPro: 2.313 ± 0.051	1.322AsnGln: 1.322 ± 0.037	2.241AsnArg: 2.241 ± 0.054	3.637AsnSer: 3.637 ± 0.073	2.656AsnThr: 2.656 ± 0.055	3.537AsnVal: 3.537 ± 0.069	0.447AsnTrp: 0.447 ± 0.02	2.555AsnTyr: 2.555 ± 0.06	0.0AsnXaa: 0.0 ± 0.0
Pro	1.51ProAla: 1.51 ± 0.039	0.307ProCys: 0.307 ± 0.02	1.865ProAsp: 1.865 ± 0.05	2.505ProGlu: 2.505 ± 0.06	1.522ProPhe: 1.522 ± 0.041	1.887ProGly: 1.887 ± 0.052	0.591ProHis: 0.591 ± 0.03	2.834ProIle: 2.834 ± 0.054	2.336ProLys: 2.336 ± 0.05	2.719ProLeu: 2.719 ± 0.058	0.756ProMet: 0.756 ± 0.025	1.51ProAsn: 1.51 ± 0.041	0.78ProPro: 0.78 ± 0.032	0.949ProGln: 0.949 ± 0.026	0.877ProArg: 0.877 ± 0.032	1.872ProSer: 1.872 ± 0.044	1.552ProThr: 1.552 ± 0.039	2.328ProVal: 2.328 ± 0.052	0.244ProTrp: 0.244 ± 0.017	1.401ProTyr: 1.401 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	1.316GlnAla: 1.316 ± 0.05	0.232GlnCys: 0.232 ± 0.016	1.159GlnAsp: 1.159 ± 0.036	1.7GlnGlu: 1.7 ± 0.039	0.917GlnPhe: 0.917 ± 0.03	1.554GlnGly: 1.554 ± 0.041	0.268GlnHis: 0.268 ± 0.015	2.32GlnIle: 2.32 ± 0.053	2.124GlnLys: 2.124 ± 0.053	2.07GlnLeu: 2.07 ± 0.052	0.681GlnMet: 0.681 ± 0.027	1.479GlnAsn: 1.479 ± 0.04	0.62GlnPro: 0.62 ± 0.026	0.69GlnGln: 0.69 ± 0.032	1.061GlnArg: 1.061 ± 0.035	1.312GlnSer: 1.312 ± 0.039	1.102GlnThr: 1.102 ± 0.037	1.403GlnVal: 1.403 ± 0.038	0.201GlnTrp: 0.201 ± 0.016	0.995GlnTyr: 0.995 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	1.984ArgAla: 1.984 ± 0.046	0.361ArgCys: 0.361 ± 0.021	2.138ArgAsp: 2.138 ± 0.047	3.296ArgGlu: 3.296 ± 0.071	1.482ArgPhe: 1.482 ± 0.046	2.368ArgGly: 2.368 ± 0.049	0.475ArgHis: 0.475 ± 0.021	3.852ArgIle: 3.852 ± 0.068	3.609ArgLys: 3.609 ± 0.065	3.295ArgLeu: 3.295 ± 0.057	1.057ArgMet: 1.057 ± 0.035	2.285ArgAsn: 2.285 ± 0.046	1.04ArgPro: 1.04 ± 0.037	0.989ArgGln: 0.989 ± 0.035	1.762ArgArg: 1.762 ± 0.048	1.697ArgSer: 1.697 ± 0.038	1.729ArgThr: 1.729 ± 0.043	2.247ArgVal: 2.247 ± 0.054	0.253ArgTrp: 0.253 ± 0.016	1.626ArgTyr: 1.626 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	3.169SerAla: 3.169 ± 0.055	0.621SerCys: 0.621 ± 0.031	3.295SerAsp: 3.295 ± 0.06	3.975SerGlu: 3.975 ± 0.075	3.077SerPhe: 3.077 ± 0.055	4.583SerGly: 4.583 ± 0.074	0.936SerHis: 0.936 ± 0.034	6.177SerIle: 6.177 ± 0.08	5.004SerLys: 5.004 ± 0.072	5.932SerLeu: 5.932 ± 0.08	1.674SerMet: 1.674 ± 0.045	3.162SerAsn: 3.162 ± 0.066	1.857SerPro: 1.857 ± 0.044	1.849SerGln: 1.849 ± 0.044	2.267SerArg: 2.267 ± 0.053	3.976SerSer: 3.976 ± 0.072	2.994SerThr: 2.994 ± 0.067	3.887SerVal: 3.887 ± 0.076	0.477SerTrp: 0.477 ± 0.022	2.624SerTyr: 2.624 ± 0.052	0.0SerXaa: 0.0 ± 0.0
Thr	2.983ThrAla: 2.983 ± 0.063	0.433ThrCys: 0.433 ± 0.021	2.755ThrAsp: 2.755 ± 0.049	3.267ThrGlu: 3.267 ± 0.065	2.141ThrPhe: 2.141 ± 0.048	3.812ThrGly: 3.812 ± 0.069	0.746ThrHis: 0.746 ± 0.027	4.431ThrIle: 4.431 ± 0.078	3.454ThrLys: 3.454 ± 0.058	4.37ThrLeu: 4.37 ± 0.077	1.075ThrMet: 1.075 ± 0.039	2.269ThrAsn: 2.269 ± 0.054	1.805ThrPro: 1.805 ± 0.04	1.082ThrGln: 1.082 ± 0.033	1.524ThrArg: 1.524 ± 0.039	2.796ThrSer: 2.796 ± 0.059	2.361ThrThr: 2.361 ± 0.053	3.78ThrVal: 3.78 ± 0.074	0.361ThrTrp: 0.361 ± 0.02	1.709ThrTyr: 1.709 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	3.567ValAla: 3.567 ± 0.08	0.743ValCys: 0.743 ± 0.025	3.731ValAsp: 3.731 ± 0.066	4.439ValGlu: 4.439 ± 0.072	3.014ValPhe: 3.014 ± 0.058	4.438ValGly: 4.438 ± 0.077	0.815ValHis: 0.815 ± 0.03	5.956ValIle: 5.956 ± 0.095	5.393ValLys: 5.393 ± 0.071	5.977ValLeu: 5.977 ± 0.082	1.581ValMet: 1.581 ± 0.043	3.409ValAsn: 3.409 ± 0.07	2.273ValPro: 2.273 ± 0.059	1.405ValGln: 1.405 ± 0.039	2.196ValArg: 2.196 ± 0.058	4.44ValSer: 4.44 ± 0.073	3.135ValThr: 3.135 ± 0.067	4.214ValVal: 4.214 ± 0.077	0.354ValTrp: 0.354 ± 0.017	2.486ValTyr: 2.486 ± 0.053	0.0ValXaa: 0.0 ± 0.0
Trp	0.422TrpAla: 0.422 ± 0.026	0.081TrpCys: 0.081 ± 0.009	0.412TrpAsp: 0.412 ± 0.024	0.499TrpGlu: 0.499 ± 0.022	0.308TrpPhe: 0.308 ± 0.019	0.536TrpGly: 0.536 ± 0.025	0.115TrpHis: 0.115 ± 0.012	0.655TrpIle: 0.655 ± 0.03	0.555TrpLys: 0.555 ± 0.024	0.627TrpLeu: 0.627 ± 0.026	0.216TrpMet: 0.216 ± 0.014	0.45TrpAsn: 0.45 ± 0.022	0.189TrpPro: 0.189 ± 0.016	0.209TrpGln: 0.209 ± 0.014	0.272TrpArg: 0.272 ± 0.016	0.367TrpSer: 0.367 ± 0.021	0.343TrpThr: 0.343 ± 0.022	0.4TrpVal: 0.4 ± 0.022	0.082TrpTrp: 0.082 ± 0.009	0.301TrpTyr: 0.301 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.838TyrAla: 1.838 ± 0.049	0.494TyrCys: 0.494 ± 0.023	2.482TyrAsp: 2.482 ± 0.056	2.971TyrGlu: 2.971 ± 0.064	2.09TyrPhe: 2.09 ± 0.052	2.826TyrGly: 2.826 ± 0.05	0.593TyrHis: 0.593 ± 0.029	4.156TyrIle: 4.156 ± 0.07	3.632TyrLys: 3.632 ± 0.068	3.735TyrLeu: 3.735 ± 0.07	1.01TyrMet: 1.01 ± 0.033	2.555TyrAsn: 2.555 ± 0.063	1.329TyrPro: 1.329 ± 0.042	0.933TyrGln: 0.933 ± 0.034	1.601TyrArg: 1.601 ± 0.04	2.656TyrSer: 2.656 ± 0.053	1.952TyrThr: 1.952 ± 0.051	2.235TyrVal: 2.235 ± 0.05	0.312TyrTrp: 0.312 ± 0.018	2.002TyrTyr: 2.002 ± 0.052	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3134 proteins (970558 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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