Amino acid dipepetide frequency for Roseobacter phage DSS3P8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.806AlaAla: 13.806 ± 1.315
1.081AlaCys: 1.081 ± 0.175
6.903AlaAsp: 6.903 ± 0.386
7.419AlaGlu: 7.419 ± 0.438
3.611AlaPhe: 3.611 ± 0.295
7.886AlaGly: 7.886 ± 0.775
1.548AlaHis: 1.548 ± 0.195
5.699AlaIle: 5.699 ± 0.407
4.741AlaLys: 4.741 ± 0.424
8.819AlaLeu: 8.819 ± 0.637
4.348AlaMet: 4.348 ± 0.405
3.292AlaAsn: 3.292 ± 0.29
3.857AlaPro: 3.857 ± 0.305
3.341AlaGln: 3.341 ± 0.337
6.657AlaArg: 6.657 ± 0.505
5.257AlaSer: 5.257 ± 0.443
5.969AlaThr: 5.969 ± 0.445
7.394AlaVal: 7.394 ± 0.397
1.351AlaTrp: 1.351 ± 0.162
3.366AlaTyr: 3.366 ± 0.289
0.0AlaXaa: 0.0 ± 0.0
Cys
1.204CysAla: 1.204 ± 0.172
0.221CysCys: 0.221 ± 0.073
0.884CysAsp: 0.884 ± 0.137
0.811CysGlu: 0.811 ± 0.175
0.344CysPhe: 0.344 ± 0.104
1.007CysGly: 1.007 ± 0.182
0.27CysHis: 0.27 ± 0.082
0.59CysIle: 0.59 ± 0.134
0.467CysLys: 0.467 ± 0.135
0.737CysLeu: 0.737 ± 0.146
0.295CysMet: 0.295 ± 0.09
0.27CysAsn: 0.27 ± 0.093
0.835CysPro: 0.835 ± 0.166
0.295CysGln: 0.295 ± 0.081
0.59CysArg: 0.59 ± 0.109
0.54CysSer: 0.54 ± 0.117
0.639CysThr: 0.639 ± 0.136
0.59CysVal: 0.59 ± 0.108
0.098CysTrp: 0.098 ± 0.048
0.295CysTyr: 0.295 ± 0.098
0.0CysXaa: 0.0 ± 0.0
Asp
7.984AspAla: 7.984 ± 0.445
0.835AspCys: 0.835 ± 0.141
4.029AspAsp: 4.029 ± 0.409
4.594AspGlu: 4.594 ± 0.379
2.997AspPhe: 2.997 ± 0.246
6.068AspGly: 6.068 ± 0.431
1.277AspHis: 1.277 ± 0.17
3.881AspIle: 3.881 ± 0.291
2.555AspLys: 2.555 ± 0.241
4.987AspLeu: 4.987 ± 0.35
2.137AspMet: 2.137 ± 0.275
2.309AspAsn: 2.309 ± 0.215
2.776AspPro: 2.776 ± 0.242
1.474AspGln: 1.474 ± 0.189
3.316AspArg: 3.316 ± 0.288
3.316AspSer: 3.316 ± 0.3
3.881AspThr: 3.881 ± 0.324
5.134AspVal: 5.134 ± 0.352
1.449AspTrp: 1.449 ± 0.235
2.555AspTyr: 2.555 ± 0.282
0.0AspXaa: 0.0 ± 0.0
Glu
7.198GluAla: 7.198 ± 0.485
0.639GluCys: 0.639 ± 0.149
4.962GluAsp: 4.962 ± 0.469
5.576GluGlu: 5.576 ± 0.443
2.997GluPhe: 2.997 ± 0.252
5.11GluGly: 5.11 ± 0.378
1.769GluHis: 1.769 ± 0.247
4.348GluIle: 4.348 ± 0.37
2.874GluLys: 2.874 ± 0.321
5.011GluLeu: 5.011 ± 0.443
2.506GluMet: 2.506 ± 0.273
2.358GluAsn: 2.358 ± 0.246
2.358GluPro: 2.358 ± 0.299
2.064GluGln: 2.064 ± 0.233
3.513GluArg: 3.513 ± 0.328
3.267GluSer: 3.267 ± 0.392
3.267GluThr: 3.267 ± 0.282
4.864GluVal: 4.864 ± 0.376
1.425GluTrp: 1.425 ± 0.233
2.358GluTyr: 2.358 ± 0.291
0.0GluXaa: 0.0 ± 0.0
Phe
3.636PheAla: 3.636 ± 0.282
0.565PheCys: 0.565 ± 0.112
3.095PheAsp: 3.095 ± 0.275
3.071PheGlu: 3.071 ± 0.259
1.744PhePhe: 1.744 ± 0.265
3.194PheGly: 3.194 ± 0.299
0.909PheHis: 0.909 ± 0.167
1.793PheIle: 1.793 ± 0.186
1.818PheLys: 1.818 ± 0.245
2.457PheLeu: 2.457 ± 0.249
1.007PheMet: 1.007 ± 0.151
1.621PheAsn: 1.621 ± 0.258
1.548PhePro: 1.548 ± 0.186
0.983PheGln: 0.983 ± 0.15
2.653PheArg: 2.653 ± 0.242
1.892PheSer: 1.892 ± 0.217
2.997PheThr: 2.997 ± 0.326
2.235PheVal: 2.235 ± 0.243
0.565PheTrp: 0.565 ± 0.132
1.351PheTyr: 1.351 ± 0.152
0.0PheXaa: 0.0 ± 0.0
Gly
7.714GlyAla: 7.714 ± 0.78
0.786GlyCys: 0.786 ± 0.134
4.913GlyAsp: 4.913 ± 0.345
5.306GlyGlu: 5.306 ± 0.362
2.727GlyPhe: 2.727 ± 0.27
5.847GlyGly: 5.847 ± 0.743
1.523GlyHis: 1.523 ± 0.211
3.906GlyIle: 3.906 ± 0.334
4.274GlyLys: 4.274 ± 0.32
5.994GlyLeu: 5.994 ± 0.471
2.555GlyMet: 2.555 ± 0.25
2.53GlyAsn: 2.53 ± 0.207
2.383GlyPro: 2.383 ± 0.295
2.064GlyGln: 2.064 ± 0.2
3.931GlyArg: 3.931 ± 0.312
4.913GlySer: 4.913 ± 0.346
5.626GlyThr: 5.626 ± 0.53
5.847GlyVal: 5.847 ± 0.371
1.67GlyTrp: 1.67 ± 0.214
3.022GlyTyr: 3.022 ± 0.28
0.0GlyXaa: 0.0 ± 0.0
His
2.064HisAla: 2.064 ± 0.196
0.368HisCys: 0.368 ± 0.114
1.499HisAsp: 1.499 ± 0.192
1.548HisGlu: 1.548 ± 0.188
0.835HisPhe: 0.835 ± 0.135
1.793HisGly: 1.793 ± 0.243
0.663HisHis: 0.663 ± 0.128
0.958HisIle: 0.958 ± 0.158
1.081HisLys: 1.081 ± 0.205
1.425HisLeu: 1.425 ± 0.202
0.491HisMet: 0.491 ± 0.12
0.59HisAsn: 0.59 ± 0.121
1.13HisPro: 1.13 ± 0.177
0.59HisGln: 0.59 ± 0.12
1.13HisArg: 1.13 ± 0.165
1.032HisSer: 1.032 ± 0.171
1.302HisThr: 1.302 ± 0.154
1.228HisVal: 1.228 ± 0.174
0.418HisTrp: 0.418 ± 0.11
0.59HisTyr: 0.59 ± 0.107
0.0HisXaa: 0.0 ± 0.0
Ile
5.503IleAla: 5.503 ± 0.397
0.491IleCys: 0.491 ± 0.133
4.348IleAsp: 4.348 ± 0.315
4.422IleGlu: 4.422 ± 0.335
1.867IlePhe: 1.867 ± 0.225
4.004IleGly: 4.004 ± 0.282
1.204IleHis: 1.204 ± 0.185
3.144IleIle: 3.144 ± 0.281
2.186IleLys: 2.186 ± 0.217
3.611IleLeu: 3.611 ± 0.255
1.646IleMet: 1.646 ± 0.226
2.186IleAsn: 2.186 ± 0.196
2.85IlePro: 2.85 ± 0.277
1.376IleGln: 1.376 ± 0.159
3.12IleArg: 3.12 ± 0.314
3.144IleSer: 3.144 ± 0.319
3.513IleThr: 3.513 ± 0.35
3.488IleVal: 3.488 ± 0.297
0.663IleTrp: 0.663 ± 0.145
1.548IleTyr: 1.548 ± 0.204
0.0IleXaa: 0.0 ± 0.0
Lys
5.233LysAla: 5.233 ± 0.389
0.319LysCys: 0.319 ± 0.086
2.997LysAsp: 2.997 ± 0.262
3.218LysGlu: 3.218 ± 0.324
1.646LysPhe: 1.646 ± 0.186
2.874LysGly: 2.874 ± 0.253
1.179LysHis: 1.179 ± 0.171
2.407LysIle: 2.407 ± 0.259
2.481LysLys: 2.481 ± 0.309
3.513LysLeu: 3.513 ± 0.339
1.425LysMet: 1.425 ± 0.203
1.523LysAsn: 1.523 ± 0.2
1.621LysPro: 1.621 ± 0.208
1.474LysGln: 1.474 ± 0.168
3.488LysArg: 3.488 ± 0.327
2.506LysSer: 2.506 ± 0.258
2.432LysThr: 2.432 ± 0.296
3.144LysVal: 3.144 ± 0.239
0.786LysTrp: 0.786 ± 0.147
1.056LysTyr: 1.056 ± 0.15
0.0LysXaa: 0.0 ± 0.0
Leu
6.952LeuAla: 6.952 ± 0.443
0.835LeuCys: 0.835 ± 0.145
5.159LeuAsp: 5.159 ± 0.341
5.159LeuGlu: 5.159 ± 0.381
2.874LeuPhe: 2.874 ± 0.288
5.159LeuGly: 5.159 ± 0.517
1.351LeuHis: 1.351 ± 0.213
3.857LeuIle: 3.857 ± 0.328
3.095LeuLys: 3.095 ± 0.303
5.503LeuLeu: 5.503 ± 0.393
1.941LeuMet: 1.941 ± 0.234
2.555LeuAsn: 2.555 ± 0.209
3.537LeuPro: 3.537 ± 0.246
2.604LeuGln: 2.604 ± 0.412
5.134LeuArg: 5.134 ± 0.331
5.11LeuSer: 5.11 ± 0.388
4.471LeuThr: 4.471 ± 0.314
4.889LeuVal: 4.889 ± 0.378
0.934LeuTrp: 0.934 ± 0.152
2.235LeuTyr: 2.235 ± 0.233
0.0LeuXaa: 0.0 ± 0.0
Met
3.611MetAla: 3.611 ± 0.35
0.319MetCys: 0.319 ± 0.086
1.793MetAsp: 1.793 ± 0.217
2.457MetGlu: 2.457 ± 0.203
1.081MetPhe: 1.081 ± 0.184
2.506MetGly: 2.506 ± 0.256
0.59MetHis: 0.59 ± 0.118
1.916MetIle: 1.916 ± 0.218
1.67MetLys: 1.67 ± 0.208
1.99MetLeu: 1.99 ± 0.21
0.909MetMet: 0.909 ± 0.158
1.13MetAsn: 1.13 ± 0.181
1.695MetPro: 1.695 ± 0.167
1.155MetGln: 1.155 ± 0.15
2.113MetArg: 2.113 ± 0.259
2.014MetSer: 2.014 ± 0.201
2.432MetThr: 2.432 ± 0.23
1.99MetVal: 1.99 ± 0.225
0.295MetTrp: 0.295 ± 0.085
0.59MetTyr: 0.59 ± 0.112
0.0MetXaa: 0.0 ± 0.0
Asn
3.955AsnAla: 3.955 ± 0.319
0.516AsnCys: 0.516 ± 0.084
2.53AsnAsp: 2.53 ± 0.27
1.916AsnGlu: 1.916 ± 0.198
1.228AsnPhe: 1.228 ± 0.221
3.267AsnGly: 3.267 ± 0.309
0.909AsnHis: 0.909 ± 0.158
1.72AsnIle: 1.72 ± 0.215
1.155AsnLys: 1.155 ± 0.19
2.629AsnLeu: 2.629 ± 0.24
1.032AsnMet: 1.032 ± 0.155
1.376AsnAsn: 1.376 ± 0.226
2.039AsnPro: 2.039 ± 0.301
1.007AsnGln: 1.007 ± 0.135
2.064AsnArg: 2.064 ± 0.203
1.818AsnSer: 1.818 ± 0.191
2.334AsnThr: 2.334 ± 0.237
2.629AsnVal: 2.629 ± 0.265
0.762AsnTrp: 0.762 ± 0.126
0.737AsnTyr: 0.737 ± 0.125
0.0AsnXaa: 0.0 ± 0.0
Pro
4.225ProAla: 4.225 ± 0.268
0.59ProCys: 0.59 ± 0.118
3.292ProAsp: 3.292 ± 0.351
3.022ProGlu: 3.022 ± 0.282
1.941ProPhe: 1.941 ± 0.261
2.948ProGly: 2.948 ± 0.234
0.909ProHis: 0.909 ± 0.141
2.137ProIle: 2.137 ± 0.225
1.965ProLys: 1.965 ± 0.226
2.555ProLeu: 2.555 ± 0.204
1.474ProMet: 1.474 ± 0.202
1.4ProAsn: 1.4 ± 0.178
1.597ProPro: 1.597 ± 0.234
1.007ProGln: 1.007 ± 0.156
2.014ProArg: 2.014 ± 0.224
2.432ProSer: 2.432 ± 0.263
3.439ProThr: 3.439 ± 0.333
3.415ProVal: 3.415 ± 0.282
0.59ProTrp: 0.59 ± 0.108
1.72ProTyr: 1.72 ± 0.212
0.0ProXaa: 0.0 ± 0.0
Gln
3.415GlnAla: 3.415 ± 0.5
0.246GlnCys: 0.246 ± 0.077
1.572GlnAsp: 1.572 ± 0.19
1.351GlnGlu: 1.351 ± 0.211
1.302GlnPhe: 1.302 ± 0.18
1.818GlnGly: 1.818 ± 0.255
0.712GlnHis: 0.712 ± 0.165
1.523GlnIle: 1.523 ± 0.207
1.621GlnLys: 1.621 ± 0.187
1.965GlnLeu: 1.965 ± 0.227
0.86GlnMet: 0.86 ± 0.129
1.105GlnAsn: 1.105 ± 0.16
1.13GlnPro: 1.13 ± 0.192
0.983GlnGln: 0.983 ± 0.264
2.235GlnArg: 2.235 ± 0.275
1.4GlnSer: 1.4 ± 0.197
1.621GlnThr: 1.621 ± 0.199
2.186GlnVal: 2.186 ± 0.268
0.639GlnTrp: 0.639 ± 0.144
0.983GlnTyr: 0.983 ± 0.181
0.0GlnXaa: 0.0 ± 0.0
Arg
6.019ArgAla: 6.019 ± 0.474
0.614ArgCys: 0.614 ± 0.141
4.324ArgAsp: 4.324 ± 0.411
4.299ArgGlu: 4.299 ± 0.375
2.874ArgPhe: 2.874 ± 0.282
4.274ArgGly: 4.274 ± 0.342
1.228ArgHis: 1.228 ± 0.197
3.366ArgIle: 3.366 ± 0.35
3.39ArgLys: 3.39 ± 0.377
4.225ArgLeu: 4.225 ± 0.329
2.014ArgMet: 2.014 ± 0.194
2.53ArgAsn: 2.53 ± 0.282
2.064ArgPro: 2.064 ± 0.284
1.695ArgGln: 1.695 ± 0.183
3.881ArgArg: 3.881 ± 0.311
2.85ArgSer: 2.85 ± 0.28
3.39ArgThr: 3.39 ± 0.291
4.053ArgVal: 4.053 ± 0.331
1.007ArgTrp: 1.007 ± 0.146
1.646ArgTyr: 1.646 ± 0.215
0.0ArgXaa: 0.0 ± 0.0
Ser
5.994SerAla: 5.994 ± 0.511
0.663SerCys: 0.663 ± 0.143
3.341SerAsp: 3.341 ± 0.282
3.144SerGlu: 3.144 ± 0.254
2.137SerPhe: 2.137 ± 0.229
5.208SerGly: 5.208 ± 0.447
1.056SerHis: 1.056 ± 0.148
3.292SerIle: 3.292 ± 0.277
2.358SerLys: 2.358 ± 0.254
4.471SerLeu: 4.471 ± 0.302
1.744SerMet: 1.744 ± 0.195
2.162SerAsn: 2.162 ± 0.283
2.407SerPro: 2.407 ± 0.284
1.597SerGln: 1.597 ± 0.208
3.366SerArg: 3.366 ± 0.27
3.636SerSer: 3.636 ± 0.497
3.439SerThr: 3.439 ± 0.418
3.562SerVal: 3.562 ± 0.291
0.762SerTrp: 0.762 ± 0.165
2.064SerTyr: 2.064 ± 0.228
0.0SerXaa: 0.0 ± 0.0
Thr
6.436ThrAla: 6.436 ± 0.533
0.639ThrCys: 0.639 ± 0.136
3.341ThrAsp: 3.341 ± 0.407
3.587ThrGlu: 3.587 ± 0.287
2.948ThrPhe: 2.948 ± 0.285
5.404ThrGly: 5.404 ± 0.395
1.277ThrHis: 1.277 ± 0.191
4.102ThrIle: 4.102 ± 0.363
2.26ThrLys: 2.26 ± 0.261
4.766ThrLeu: 4.766 ± 0.343
2.014ThrMet: 2.014 ± 0.238
2.358ThrAsn: 2.358 ± 0.24
3.808ThrPro: 3.808 ± 0.36
1.376ThrGln: 1.376 ± 0.181
3.267ThrArg: 3.267 ± 0.29
3.857ThrSer: 3.857 ± 0.408
3.685ThrThr: 3.685 ± 0.371
4.938ThrVal: 4.938 ± 0.453
0.786ThrTrp: 0.786 ± 0.12
1.965ThrTyr: 1.965 ± 0.211
0.0ThrXaa: 0.0 ± 0.0
Val
6.829ValAla: 6.829 ± 0.511
0.737ValCys: 0.737 ± 0.134
5.036ValAsp: 5.036 ± 0.293
4.717ValGlu: 4.717 ± 0.378
2.334ValPhe: 2.334 ± 0.245
4.987ValGly: 4.987 ± 0.346
1.081ValHis: 1.081 ± 0.162
3.144ValIle: 3.144 ± 0.261
3.095ValLys: 3.095 ± 0.265
4.815ValLeu: 4.815 ± 0.341
2.334ValMet: 2.334 ± 0.257
2.653ValAsn: 2.653 ± 0.26
3.144ValPro: 3.144 ± 0.307
2.211ValGln: 2.211 ± 0.244
4.766ValArg: 4.766 ± 0.289
4.446ValSer: 4.446 ± 0.338
5.404ValThr: 5.404 ± 0.462
4.668ValVal: 4.668 ± 0.419
1.253ValTrp: 1.253 ± 0.16
2.285ValTyr: 2.285 ± 0.249
0.0ValXaa: 0.0 ± 0.0
Trp
1.4TrpAla: 1.4 ± 0.198
0.123TrpCys: 0.123 ± 0.054
1.277TrpAsp: 1.277 ± 0.187
1.007TrpGlu: 1.007 ± 0.145
0.516TrpPhe: 0.516 ± 0.129
1.179TrpGly: 1.179 ± 0.169
0.442TrpHis: 0.442 ± 0.101
0.983TrpIle: 0.983 ± 0.157
0.688TrpLys: 0.688 ± 0.118
1.302TrpLeu: 1.302 ± 0.16
0.59TrpMet: 0.59 ± 0.113
0.639TrpAsn: 0.639 ± 0.13
0.59TrpPro: 0.59 ± 0.117
0.491TrpGln: 0.491 ± 0.111
0.811TrpArg: 0.811 ± 0.152
1.032TrpSer: 1.032 ± 0.158
1.081TrpThr: 1.081 ± 0.191
1.179TrpVal: 1.179 ± 0.157
0.295TrpTrp: 0.295 ± 0.088
0.565TrpTyr: 0.565 ± 0.114
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.169TyrAla: 3.169 ± 0.33
0.393TyrCys: 0.393 ± 0.109
2.26TyrAsp: 2.26 ± 0.241
1.842TyrGlu: 1.842 ± 0.188
1.13TyrPhe: 1.13 ± 0.16
2.997TyrGly: 2.997 ± 0.304
0.86TyrHis: 0.86 ± 0.156
1.548TyrIle: 1.548 ± 0.252
1.548TyrLys: 1.548 ± 0.221
2.579TyrLeu: 2.579 ± 0.238
0.909TyrMet: 0.909 ± 0.126
1.032TyrAsn: 1.032 ± 0.15
1.302TyrPro: 1.302 ± 0.179
0.884TyrGln: 0.884 ± 0.154
1.695TyrArg: 1.695 ± 0.227
1.941TyrSer: 1.941 ± 0.205
1.892TyrThr: 1.892 ± 0.267
2.506TyrVal: 2.506 ± 0.284
0.442TyrTrp: 0.442 ± 0.1
1.032TyrTyr: 1.032 ± 0.162
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 229 proteins (40708 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski