Clostridium homopropionicum DSM 5847

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium homopropionicum

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3499 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L6Z9S4|A0A0L6Z9S4_9CLOT Uncharacterized protein OS=Clostridium homopropionicum DSM 5847 OX=1121318 GN=CLHOM_17990 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.2AYY4 pKa = 10.42VDD6 pKa = 3.52QEE8 pKa = 4.39TCIGCGLCPSICPEE22 pKa = 4.05VFDD25 pKa = 5.14MGDD28 pKa = 3.28DD29 pKa = 3.94GKK31 pKa = 11.2AHH33 pKa = 7.26AIVDD37 pKa = 4.16EE38 pKa = 4.53VPSGSEE44 pKa = 3.74DD45 pKa = 3.59GAVEE49 pKa = 4.18SQEE52 pKa = 4.23SCPVDD57 pKa = 4.07AIKK60 pKa = 10.96VEE62 pKa = 4.34EE63 pKa = 4.07

Molecular weight: 6.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L6Z664|A0A0L6Z664_9CLOT Alcohol dehydrogenase 2 OS=Clostridium homopropionicum DSM 5847 OX=1121318 GN=adhB PE=4 SV=1
MM1 pKa = 7.38NYY3 pKa = 9.89LRR5 pKa = 11.84RR6 pKa = 11.84FMMGRR11 pKa = 11.84YY12 pKa = 9.71GSDD15 pKa = 3.32QLSMALILLSILLSMISGLIHH36 pKa = 7.14LKK38 pKa = 9.82LLRR41 pKa = 11.84YY42 pKa = 9.3IADD45 pKa = 3.41IPLVISIFRR54 pKa = 11.84ILSRR58 pKa = 11.84DD59 pKa = 2.82ISKK62 pKa = 10.67RR63 pKa = 11.84RR64 pKa = 11.84MEE66 pKa = 4.12NYY68 pKa = 9.22KK69 pKa = 10.44FSMLMSPVYY78 pKa = 10.47SWLKK82 pKa = 7.98KK83 pKa = 6.35TQRR86 pKa = 11.84HH87 pKa = 5.94AIDD90 pKa = 3.92SRR92 pKa = 11.84THH94 pKa = 7.33RR95 pKa = 11.84YY96 pKa = 8.0FKK98 pKa = 10.82CPNCRR103 pKa = 11.84TQLRR107 pKa = 11.84VPKK110 pKa = 9.9GKK112 pKa = 10.51GKK114 pKa = 10.34IIITCPKK121 pKa = 9.31CKK123 pKa = 9.96TEE125 pKa = 3.85FRR127 pKa = 11.84EE128 pKa = 4.34RR129 pKa = 11.84TT130 pKa = 3.49

Molecular weight: 15.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3499

0

3499

1063634

29

3130

304.0

34.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.926 ± 0.047

1.137 ± 0.019

5.276 ± 0.03

7.592 ± 0.048

4.338 ± 0.032

6.401 ± 0.045

1.361 ± 0.016

10.073 ± 0.051

9.017 ± 0.04

9.08 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.583 ± 0.023

6.145 ± 0.042

2.866 ± 0.021

2.439 ± 0.022

3.415 ± 0.032

6.379 ± 0.038

4.897 ± 0.04

6.365 ± 0.035

0.669 ± 0.012

4.041 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski