Burkholderia sp. TSV86

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia; pseudomallei group

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4805 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A103EB67|A0A103EB67_9BURK Plasmid stabilization protein OS=Burkholderia sp. TSV86 OX=1385594 GN=WS68_16740 PE=4 SV=1
MM1 pKa = 8.07PDD3 pKa = 3.57LANIDD8 pKa = 3.83SSSIQQWLTEE18 pKa = 4.5RR19 pKa = 11.84IARR22 pKa = 11.84YY23 pKa = 8.77LQISPEE29 pKa = 4.75DD30 pKa = 3.53IGASTPLDD38 pKa = 3.98TIGLDD43 pKa = 3.44SVYY46 pKa = 11.24ALALCGEE53 pKa = 4.46IEE55 pKa = 4.27EE56 pKa = 4.58TLHH59 pKa = 6.75CVVEE63 pKa = 4.25PTIAWDD69 pKa = 4.16FPTVAEE75 pKa = 4.75IAQEE79 pKa = 3.95LCNRR83 pKa = 11.84LMADD87 pKa = 3.65GADD90 pKa = 3.34VMASEE95 pKa = 4.75KK96 pKa = 10.97

Molecular weight: 10.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A103EEC0|A0A103EEC0_9BURK LysR family transcriptional regulator OS=Burkholderia sp. TSV86 OX=1385594 GN=WS68_12635 PE=3 SV=1
MM1 pKa = 7.92PFRR4 pKa = 11.84LPSYY8 pKa = 9.35LHH10 pKa = 6.41RR11 pKa = 11.84TTKK14 pKa = 10.04GARR17 pKa = 11.84FASPRR22 pKa = 11.84ITRR25 pKa = 11.84ALRR28 pKa = 11.84HH29 pKa = 5.48HH30 pKa = 6.11SARR33 pKa = 11.84ANVLAEE39 pKa = 4.0RR40 pKa = 11.84ARR42 pKa = 11.84WHH44 pKa = 5.6QPRR47 pKa = 11.84LDD49 pKa = 3.82GLRR52 pKa = 11.84TWLHH56 pKa = 6.34ALLSMMRR63 pKa = 11.84AQAAARR69 pKa = 11.84RR70 pKa = 11.84TALRR74 pKa = 11.84SLLPKK79 pKa = 10.2PSSLRR84 pKa = 11.84PNAQPARR91 pKa = 11.84RR92 pKa = 11.84ASAAARR98 pKa = 11.84RR99 pKa = 11.84INRR102 pKa = 11.84PRR104 pKa = 11.84RR105 pKa = 11.84LATSTGWFAFAARR118 pKa = 4.17

Molecular weight: 13.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4805

0

4805

1537092

27

5230

319.9

34.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.795 ± 0.073

0.967 ± 0.011

5.663 ± 0.027

4.767 ± 0.033

3.594 ± 0.027

8.197 ± 0.038

2.341 ± 0.017

4.657 ± 0.026

2.937 ± 0.034

10.057 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.015

2.763 ± 0.031

5.214 ± 0.03

3.506 ± 0.023

7.358 ± 0.042

5.469 ± 0.032

5.198 ± 0.029

7.432 ± 0.029

1.376 ± 0.015

2.511 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski