Cryptococcus depauperatus CBS 7841

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes; Tremellales; Cryptococcaceae; Cryptococcus; Cryptococcus depauperatus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6530 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E3HV80|A0A1E3HV80_9TREE Ribosomal RNA-processing protein 1 OS=Cryptococcus depauperatus CBS 7841 OX=1295531 GN=L203_05835 PE=4 SV=1
MM1 pKa = 7.89PSHH4 pKa = 7.18FVPTTPSSGYY14 pKa = 10.62SPDD17 pKa = 4.13SPSQTNSDD25 pKa = 3.81QPRR28 pKa = 11.84VILMPVSEE36 pKa = 4.73SSLGGDD42 pKa = 3.49GDD44 pKa = 4.24ALSSVLTIDD53 pKa = 4.25PYY55 pKa = 10.93IATSPYY61 pKa = 8.69DD62 pKa = 3.56TAGSSVPSSTTATLCLIVLISDD84 pKa = 3.52DD85 pKa = 3.8TTGNNSPISPSITTDD100 pKa = 3.16SASTPNKK107 pKa = 10.26RR108 pKa = 11.84LDD110 pKa = 3.61PDD112 pKa = 3.31

Molecular weight: 11.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E3HSC4|A0A1E3HSC4_9TREE CAP10 domain-containing protein OS=Cryptococcus depauperatus CBS 7841 OX=1295531 GN=L203_06032 PE=4 SV=1
MM1 pKa = 7.52ASAVGRR7 pKa = 11.84TVSRR11 pKa = 11.84LKK13 pKa = 10.96AVGLAGKK20 pKa = 8.57PATAQPTDD28 pKa = 3.16HH29 pKa = 7.05GIVDD33 pKa = 4.25GLFKK37 pKa = 10.28MEE39 pKa = 4.23KK40 pKa = 6.86TTSISPNAYY49 pKa = 10.15NKK51 pKa = 10.09FGPTLTSTRR60 pKa = 11.84HH61 pKa = 5.63KK62 pKa = 9.55QTQPYY67 pKa = 8.91SAEE70 pKa = 4.19SVDD73 pKa = 4.97SKK75 pKa = 11.3GLQNITYY82 pKa = 9.35SYY84 pKa = 10.31RR85 pKa = 11.84DD86 pKa = 3.35EE87 pKa = 4.23AHH89 pKa = 6.43RR90 pKa = 11.84QSPQPNPVTEE100 pKa = 4.26CFRR103 pKa = 11.84IAVIIPPPPIARR115 pKa = 11.84KK116 pKa = 6.61MTARR120 pKa = 11.84NGVPRR125 pKa = 11.84VSSLRR130 pKa = 11.84RR131 pKa = 11.84TISKK135 pKa = 10.05HH136 pKa = 4.46VEE138 pKa = 3.33VWPINRR144 pKa = 11.84FEE146 pKa = 4.24VQTAHH151 pKa = 6.28CVVRR155 pKa = 11.84SIRR158 pKa = 11.84SGTCRR163 pKa = 11.84HH164 pKa = 6.53IYY166 pKa = 9.05FICNSFGILHH176 pKa = 6.48TSRR179 pKa = 11.84GPSIRR184 pKa = 11.84CLIRR188 pKa = 5.25

Molecular weight: 20.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6530

0

6530

3229921

14

4945

494.6

54.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.732 ± 0.023

1.089 ± 0.011

5.349 ± 0.019

6.51 ± 0.028

3.443 ± 0.017

6.601 ± 0.03

2.402 ± 0.012

5.12 ± 0.021

5.478 ± 0.027

9.192 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.156 ± 0.009

3.757 ± 0.012

6.196 ± 0.032

4.058 ± 0.022

5.915 ± 0.022

9.225 ± 0.04

5.863 ± 0.018

6.007 ± 0.021

1.351 ± 0.012

2.556 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski