Prevotella sp. OH937_COT-195
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2205 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3P1SY30|A0A3P1SY30_9BACT Tetraacyldisaccharide 4'-kinase OS=Prevotella sp. OH937_COT-195 OX=2491051 GN=lpxK PE=3 SV=1
MM1 pKa = 7.48 AKK3 pKa = 10.21 VIEE6 pKa = 4.11 GLYY9 pKa = 9.7 YY10 pKa = 10.21 SEE12 pKa = 3.62 SHH14 pKa = 6.61 EE15 pKa = 4.2 YY16 pKa = 10.66 VKK18 pKa = 11.18 VEE20 pKa = 4.07 GNVAYY25 pKa = 9.73 IGITDD30 pKa = 3.74 YY31 pKa = 11.49 AQNALGNVVYY41 pKa = 11.14 VDD43 pKa = 3.67 MPEE46 pKa = 3.9 VDD48 pKa = 5.1 DD49 pKa = 4.73 EE50 pKa = 4.83 VEE52 pKa = 3.85 ADD54 pKa = 3.46 EE55 pKa = 6.0 DD56 pKa = 4.07 FGAVEE61 pKa = 4.38 SVKK64 pKa = 10.61 AASDD68 pKa = 3.55 LKK70 pKa = 10.96 SPVSGTVLEE79 pKa = 4.44 VNEE82 pKa = 4.04 VLEE85 pKa = 5.0 DD86 pKa = 3.52 KK87 pKa = 10.86 PEE89 pKa = 4.57 SINEE93 pKa = 3.65 DD94 pKa = 3.62 AYY96 pKa = 11.07 ANWIIKK102 pKa = 9.94 IEE104 pKa = 4.11 LSDD107 pKa = 3.64 TAEE110 pKa = 4.54 LDD112 pKa = 3.52 NLMSAEE118 pKa = 4.36 EE119 pKa = 3.96 YY120 pKa = 11.26 AKK122 pKa = 10.68 FCEE125 pKa = 4.42 AQDD128 pKa = 3.39
Molecular weight: 14.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.77
IPC_protein 3.719
Toseland 3.528
ProMoST 3.808
Dawson 3.681
Bjellqvist 3.872
Wikipedia 3.579
Rodwell 3.554
Grimsley 3.439
Solomon 3.668
Lehninger 3.63
Nozaki 3.808
DTASelect 3.948
Thurlkill 3.567
EMBOSS 3.592
Sillero 3.834
Patrickios 0.947
IPC_peptide 3.668
IPC2_peptide 3.808
IPC2.peptide.svr19 3.775
Protein with the highest isoelectric point:
>tr|A0A3P1SZ43|A0A3P1SZ43_9BACT Uncharacterized protein OS=Prevotella sp. OH937_COT-195 OX=2491051 GN=EII32_03335 PE=4 SV=1
MM1 pKa = 7.59 PLSLRR6 pKa = 11.84 FPTALARR13 pKa = 11.84 LLTSAQPLHH22 pKa = 7.14 PDD24 pKa = 3.11 AARR27 pKa = 11.84 YY28 pKa = 9.81 AFDD31 pKa = 3.31 EE32 pKa = 4.66 TAAHH36 pKa = 6.85 PLNNRR41 pKa = 11.84 TKK43 pKa = 10.41 SVEE46 pKa = 3.88 GSNDD50 pKa = 3.27 RR51 pKa = 11.84 LGLNHH56 pKa = 6.88 TLLVGNRR63 pKa = 11.84 LTCTDD68 pKa = 3.48 KK69 pKa = 11.43 SIFAAKK75 pKa = 10.08 RR76 pKa = 11.84 PVKK79 pKa = 9.94 TYY81 pKa = 8.63 LAKK84 pKa = 9.55 MRR86 pKa = 11.84 FYY88 pKa = 10.59 KK89 pKa = 10.58 TMLPKK94 pKa = 10.51 PPVLHH99 pKa = 7.15 RR100 pKa = 11.84 NFAYY104 pKa = 9.6 IAKK107 pKa = 10.06 QIGRR111 pKa = 11.84 FCRR114 pKa = 11.84 AIPAEE119 pKa = 4.05 LFHH122 pKa = 6.9 RR123 pKa = 11.84 FSFIIFF129 pKa = 3.73
Molecular weight: 14.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.333
IPC2_protein 9.853
IPC_protein 10.643
Toseland 10.804
ProMoST 10.482
Dawson 10.891
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 11.169
Grimsley 10.935
Solomon 11.008
Lehninger 10.979
Nozaki 10.789
DTASelect 10.599
Thurlkill 10.789
EMBOSS 11.199
Sillero 10.818
Patrickios 10.906
IPC_peptide 11.008
IPC2_peptide 9.604
IPC2.peptide.svr19 8.454
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2205
0
2205
722840
22
2515
327.8
36.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.063 ± 0.06
1.341 ± 0.019
5.734 ± 0.035
6.611 ± 0.05
4.475 ± 0.036
6.873 ± 0.047
1.928 ± 0.023
7.104 ± 0.045
6.915 ± 0.044
8.456 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.13 ± 0.028
5.328 ± 0.045
3.482 ± 0.026
3.108 ± 0.026
5.068 ± 0.042
5.804 ± 0.039
5.74 ± 0.044
6.662 ± 0.04
1.09 ± 0.019
4.089 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here