Stackebrandtia endophytica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Glycomycetales; Glycomycetaceae; Stackebrandtia

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5197 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A543AST5|A0A543AST5_9ACTN (R)-citramalate synthase OS=Stackebrandtia endophytica OX=1496996 GN=FB566_1164 PE=3 SV=1
MM1 pKa = 6.99TPRR4 pKa = 11.84VRR6 pKa = 11.84GVLVGAVSLAVMLTGCASEE25 pKa = 4.34SAPLVQSDD33 pKa = 4.05EE34 pKa = 4.85DD35 pKa = 4.23DD36 pKa = 3.83VAVDD40 pKa = 4.27PNSGDD45 pKa = 3.49LSAGEE50 pKa = 4.55SDD52 pKa = 3.9PVTDD56 pKa = 3.79PFYY59 pKa = 10.73PEE61 pKa = 4.92FGNADD66 pKa = 3.57IDD68 pKa = 4.07VLHH71 pKa = 6.92YY72 pKa = 11.06DD73 pKa = 4.06LDD75 pKa = 4.09LSYY78 pKa = 11.1EE79 pKa = 4.02PSNQVLTGTATLTIRR94 pKa = 11.84PVVDD98 pKa = 3.28AAEE101 pKa = 4.27VAVDD105 pKa = 3.77LAKK108 pKa = 10.6PLMVSKK114 pKa = 9.67VTVDD118 pKa = 3.33DD119 pKa = 4.33VEE121 pKa = 4.43ATFQHH126 pKa = 5.55EE127 pKa = 4.37QYY129 pKa = 11.42DD130 pKa = 4.25LVIDD134 pKa = 3.94TAVTADD140 pKa = 3.56QPVVARR146 pKa = 11.84IEE148 pKa = 4.24YY149 pKa = 10.21SGVPQTVPAPAKK161 pKa = 10.25RR162 pKa = 11.84GDD164 pKa = 3.22MSMGIGASTNAVTGALWSFQEE185 pKa = 4.21PFGALTWYY193 pKa = 9.14PVNDD197 pKa = 4.12HH198 pKa = 7.12PSDD201 pKa = 3.47EE202 pKa = 4.27ALYY205 pKa = 10.38DD206 pKa = 3.51IAITVPDD213 pKa = 5.3GYY215 pKa = 11.23SGVASGKK222 pKa = 10.12FVGQEE227 pKa = 3.13GDD229 pKa = 3.63TFHH232 pKa = 6.57WTSSDD237 pKa = 3.21PVASYY242 pKa = 8.98VTTIAVDD249 pKa = 3.54QYY251 pKa = 11.54EE252 pKa = 4.48LTEE255 pKa = 3.98LTGPNGLPITLWTLPEE271 pKa = 4.06YY272 pKa = 11.03SEE274 pKa = 4.16FMPVLEE280 pKa = 5.02RR281 pKa = 11.84MPDD284 pKa = 3.35MIEE287 pKa = 3.89WLEE290 pKa = 4.02SRR292 pKa = 11.84YY293 pKa = 10.51GPYY296 pKa = 10.41PFDD299 pKa = 3.3SAGVVLVGGEE309 pKa = 4.27SAMEE313 pKa = 4.06TQEE316 pKa = 4.45MITFSGDD323 pKa = 3.78FINLPGVDD331 pKa = 3.78ADD333 pKa = 4.0YY334 pKa = 10.8VAGVVVHH341 pKa = 6.5EE342 pKa = 5.48LAHH345 pKa = 5.21QWFGNAVSPRR355 pKa = 11.84DD356 pKa = 3.85WSNLWLNEE364 pKa = 3.5GAATYY369 pKa = 9.87IEE371 pKa = 4.4QMWNVDD377 pKa = 2.88NGYY380 pKa = 6.78TTEE383 pKa = 4.12SSVVSGWEE391 pKa = 3.54RR392 pKa = 11.84DD393 pKa = 3.6DD394 pKa = 4.1EE395 pKa = 4.59YY396 pKa = 11.71LRR398 pKa = 11.84MAYY401 pKa = 9.64GPPGAADD408 pKa = 3.63PDD410 pKa = 3.94AFASSNSYY418 pKa = 9.95VCPALMLHH426 pKa = 7.03RR427 pKa = 11.84MRR429 pKa = 11.84DD430 pKa = 3.72LLGGADD436 pKa = 4.66KK437 pKa = 11.15VDD439 pKa = 3.66EE440 pKa = 5.05LLAAWVVEE448 pKa = 3.7FDD450 pKa = 3.48NRR452 pKa = 11.84SVEE455 pKa = 3.95RR456 pKa = 11.84DD457 pKa = 3.42DD458 pKa = 5.82FVAFVNEE465 pKa = 4.35YY466 pKa = 10.2TGTDD470 pKa = 2.98HH471 pKa = 7.29TAFIDD476 pKa = 3.44EE477 pKa = 4.45WLDD480 pKa = 3.77SEE482 pKa = 4.71TTPEE486 pKa = 3.7

Molecular weight: 52.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A543AXL1|A0A543AXL1_9ACTN Acetyltransferase (GNAT) family protein OS=Stackebrandtia endophytica OX=1496996 GN=FB566_2872 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84GRR40 pKa = 11.84ARR42 pKa = 11.84LTAA45 pKa = 4.29

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5197

0

5197

1637824

29

6712

315.1

34.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.123 ± 0.04

0.795 ± 0.01

6.639 ± 0.033

5.615 ± 0.034

2.918 ± 0.02

8.715 ± 0.027

2.236 ± 0.016

4.364 ± 0.023

1.969 ± 0.027

10.003 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.088 ± 0.015

2.159 ± 0.02

5.657 ± 0.035

2.879 ± 0.021

7.466 ± 0.042

5.684 ± 0.02

6.452 ± 0.032

8.534 ± 0.03

1.565 ± 0.015

2.137 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski