Escherichia phage aalborv
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6B9WZE4|A0A6B9WZE4_9CAUD Uncharacterized protein OS=Escherichia phage aalborv OX=2696375 GN=aalborv_56 PE=4 SV=1
MM1 pKa = 6.55 MQSTVIKK8 pKa = 10.23 FLGSEE13 pKa = 4.07 CEE15 pKa = 4.04 EE16 pKa = 4.03 VNEE19 pKa = 4.61 CFTAGKK25 pKa = 9.79 RR26 pKa = 11.84 YY27 pKa = 9.13 HH28 pKa = 7.13 ADD30 pKa = 2.88 VSSAGNYY37 pKa = 8.25 FVYY40 pKa = 10.17 DD41 pKa = 3.56 DD42 pKa = 5.68 HH43 pKa = 8.26 GDD45 pKa = 3.14 TWAIDD50 pKa = 3.66 PDD52 pKa = 4.58 DD53 pKa = 5.85 DD54 pKa = 5.02 DD55 pKa = 5.81 FSVV58 pKa = 3.2
Molecular weight: 6.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.884
IPC_protein 3.821
Toseland 3.617
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.808
Rodwell 3.656
Grimsley 3.528
Solomon 3.808
Lehninger 3.77
Nozaki 3.961
DTASelect 4.202
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.948
Patrickios 0.769
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.846
Protein with the highest isoelectric point:
>tr|A0A6B9WRZ7|A0A6B9WRZ7_9CAUD Uncharacterized protein OS=Escherichia phage aalborv OX=2696375 GN=aalborv_54 PE=4 SV=1
MM1 pKa = 7.8 LDD3 pKa = 3.18 LHH5 pKa = 7.08 KK6 pKa = 10.61 IRR8 pKa = 11.84 VGTKK12 pKa = 9.21 FVVTWVYY19 pKa = 11.05 DD20 pKa = 3.78 EE21 pKa = 4.49 RR22 pKa = 11.84 CGIRR26 pKa = 11.84 KK27 pKa = 9.94 GEE29 pKa = 4.08 IVTASDD35 pKa = 4.04 RR36 pKa = 11.84 YY37 pKa = 9.95 IGSLKK42 pKa = 10.18 EE43 pKa = 3.88 FNRR46 pKa = 11.84 PRR48 pKa = 11.84 IKK50 pKa = 10.36 NGYY53 pKa = 9.12 IITRR57 pKa = 11.84 RR58 pKa = 11.84 LGFDD62 pKa = 3.8 HH63 pKa = 6.82 YY64 pKa = 10.9 CVEE67 pKa = 4.2 ATQGVLIEE75 pKa = 4.44 LKK77 pKa = 10.31 RR78 pKa = 11.84 ISDD81 pKa = 3.56 HH82 pKa = 6.68 RR83 pKa = 11.84 GCHH86 pKa = 4.72 VKK88 pKa = 9.37 TMKK91 pKa = 10.19 MPFQRR96 pKa = 11.84 ARR98 pKa = 11.84 YY99 pKa = 7.81 DD100 pKa = 3.15 ARR102 pKa = 11.84 RR103 pKa = 11.84 MRR105 pKa = 11.84 RR106 pKa = 11.84 LARR109 pKa = 11.84 NAIKK113 pKa = 10.19 FKK115 pKa = 11.06 KK116 pKa = 8.9 PDD118 pKa = 3.27 NVLYY122 pKa = 10.95 GMYY125 pKa = 10.25 KK126 pKa = 10.52 GIARR130 pKa = 11.84 NAGKK134 pKa = 10.28
Molecular weight: 15.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.328
IPC2_protein 9.78
IPC_protein 10.423
Toseland 10.643
ProMoST 10.321
Dawson 10.745
Bjellqvist 10.438
Wikipedia 10.935
Rodwell 11.082
Grimsley 10.804
Solomon 10.818
Lehninger 10.789
Nozaki 10.613
DTASelect 10.423
Thurlkill 10.643
EMBOSS 11.023
Sillero 10.672
Patrickios 10.789
IPC_peptide 10.833
IPC2_peptide 9.37
IPC2.peptide.svr19 8.576
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
71
0
71
13383
38
1141
188.5
21.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.995 ± 0.467
1.397 ± 0.187
6.321 ± 0.263
6.957 ± 0.269
3.886 ± 0.176
7.383 ± 0.276
1.778 ± 0.223
6.785 ± 0.201
7.577 ± 0.342
6.553 ± 0.194
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.228 ± 0.19
4.558 ± 0.183
3.333 ± 0.169
3.856 ± 0.289
5.029 ± 0.245
6.06 ± 0.23
5.372 ± 0.293
6.74 ± 0.21
1.412 ± 0.12
3.781 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here