Amino acid dipepetide frequency for Escherichia phage aalborv

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.352AlaAla: 6.352 ± 0.923
0.673AlaCys: 0.673 ± 0.217
4.259AlaAsp: 4.259 ± 0.666
5.53AlaGlu: 5.53 ± 0.653
2.989AlaPhe: 2.989 ± 0.551
4.633AlaGly: 4.633 ± 0.625
1.046AlaHis: 1.046 ± 0.268
5.978AlaIle: 5.978 ± 0.727
7.174AlaLys: 7.174 ± 0.991
6.576AlaLeu: 6.576 ± 0.784
3.213AlaMet: 3.213 ± 0.406
3.288AlaAsn: 3.288 ± 0.667
1.569AlaPro: 1.569 ± 0.369
3.288AlaGln: 3.288 ± 0.516
4.185AlaArg: 4.185 ± 0.63
4.932AlaSer: 4.932 ± 0.604
4.259AlaThr: 4.259 ± 0.816
6.128AlaVal: 6.128 ± 0.791
1.196AlaTrp: 1.196 ± 0.232
2.69AlaTyr: 2.69 ± 0.434
0.0AlaXaa: 0.0 ± 0.0
Cys
1.27CysAla: 1.27 ± 0.325
0.075CysCys: 0.075 ± 0.065
1.644CysAsp: 1.644 ± 0.319
1.046CysGlu: 1.046 ± 0.254
0.374CysPhe: 0.374 ± 0.153
1.719CysGly: 1.719 ± 0.381
0.299CysHis: 0.299 ± 0.133
0.673CysIle: 0.673 ± 0.225
0.897CysLys: 0.897 ± 0.3
0.971CysLeu: 0.971 ± 0.28
0.523CysMet: 0.523 ± 0.184
0.374CysAsn: 0.374 ± 0.185
0.448CysPro: 0.448 ± 0.171
0.224CysGln: 0.224 ± 0.124
0.822CysArg: 0.822 ± 0.288
0.374CysSer: 0.374 ± 0.185
0.673CysThr: 0.673 ± 0.242
0.747CysVal: 0.747 ± 0.247
0.374CysTrp: 0.374 ± 0.158
0.448CysTyr: 0.448 ± 0.176
0.0CysXaa: 0.0 ± 0.0
Asp
4.857AspAla: 4.857 ± 0.66
0.897AspCys: 0.897 ± 0.3
4.334AspAsp: 4.334 ± 0.737
4.334AspGlu: 4.334 ± 0.581
3.213AspPhe: 3.213 ± 0.53
6.277AspGly: 6.277 ± 0.697
0.897AspHis: 0.897 ± 0.247
3.437AspIle: 3.437 ± 0.524
4.484AspLys: 4.484 ± 0.56
4.558AspLeu: 4.558 ± 0.482
2.317AspMet: 2.317 ± 0.43
2.391AspAsn: 2.391 ± 0.381
2.541AspPro: 2.541 ± 0.439
1.943AspGln: 1.943 ± 0.366
2.765AspArg: 2.765 ± 0.467
3.213AspSer: 3.213 ± 0.507
3.961AspThr: 3.961 ± 0.582
3.288AspVal: 3.288 ± 0.482
1.046AspTrp: 1.046 ± 0.318
2.466AspTyr: 2.466 ± 0.449
0.0AspXaa: 0.0 ± 0.0
Glu
5.679GluAla: 5.679 ± 0.66
0.971GluCys: 0.971 ± 0.244
3.437GluAsp: 3.437 ± 0.437
4.783GluGlu: 4.783 ± 0.628
3.587GluPhe: 3.587 ± 0.588
3.437GluGly: 3.437 ± 0.457
1.345GluHis: 1.345 ± 0.355
5.679GluIle: 5.679 ± 0.62
4.334GluLys: 4.334 ± 0.589
4.932GluLeu: 4.932 ± 0.784
2.84GluMet: 2.84 ± 0.464
3.587GluAsn: 3.587 ± 0.529
1.793GluPro: 1.793 ± 0.421
3.288GluGln: 3.288 ± 0.613
2.914GluArg: 2.914 ± 0.688
4.484GluSer: 4.484 ± 0.511
3.886GluThr: 3.886 ± 0.534
4.932GluVal: 4.932 ± 0.572
0.747GluTrp: 0.747 ± 0.258
2.914GluTyr: 2.914 ± 0.456
0.0GluXaa: 0.0 ± 0.0
Phe
2.615PheAla: 2.615 ± 0.397
0.374PheCys: 0.374 ± 0.185
3.363PheAsp: 3.363 ± 0.531
2.317PheGlu: 2.317 ± 0.339
1.046PhePhe: 1.046 ± 0.355
3.064PheGly: 3.064 ± 0.49
0.448PheHis: 0.448 ± 0.157
2.466PheIle: 2.466 ± 0.357
3.213PheLys: 3.213 ± 0.399
2.317PheLeu: 2.317 ± 0.528
1.495PheMet: 1.495 ± 0.389
2.018PheAsn: 2.018 ± 0.336
1.42PhePro: 1.42 ± 0.384
1.569PheGln: 1.569 ± 0.389
2.018PheArg: 2.018 ± 0.364
2.092PheSer: 2.092 ± 0.489
2.69PheThr: 2.69 ± 0.395
2.541PheVal: 2.541 ± 0.49
0.523PheTrp: 0.523 ± 0.167
1.27PheTyr: 1.27 ± 0.424
0.0PheXaa: 0.0 ± 0.0
Gly
4.558GlyAla: 4.558 ± 0.786
1.345GlyCys: 1.345 ± 0.327
5.679GlyAsp: 5.679 ± 0.892
5.156GlyGlu: 5.156 ± 0.657
3.288GlyPhe: 3.288 ± 0.454
6.128GlyGly: 6.128 ± 0.987
1.27GlyHis: 1.27 ± 0.328
4.185GlyIle: 4.185 ± 0.56
6.95GlyLys: 6.95 ± 0.537
5.007GlyLeu: 5.007 ± 0.582
2.466GlyMet: 2.466 ± 0.439
3.512GlyAsn: 3.512 ± 0.525
0.374GlyPro: 0.374 ± 0.171
2.018GlyGln: 2.018 ± 0.346
2.615GlyArg: 2.615 ± 0.351
5.53GlySer: 5.53 ± 0.586
3.064GlyThr: 3.064 ± 0.634
5.903GlyVal: 5.903 ± 0.611
1.42GlyTrp: 1.42 ± 0.305
3.363GlyTyr: 3.363 ± 0.447
0.0GlyXaa: 0.0 ± 0.0
His
0.673HisAla: 0.673 ± 0.297
0.374HisCys: 0.374 ± 0.193
1.046HisAsp: 1.046 ± 0.286
1.121HisGlu: 1.121 ± 0.308
0.673HisPhe: 0.673 ± 0.206
1.793HisGly: 1.793 ± 0.479
0.448HisHis: 0.448 ± 0.218
1.121HisIle: 1.121 ± 0.264
1.42HisLys: 1.42 ± 0.43
0.897HisLeu: 0.897 ± 0.248
0.374HisMet: 0.374 ± 0.225
0.897HisAsn: 0.897 ± 0.306
0.523HisPro: 0.523 ± 0.213
0.598HisGln: 0.598 ± 0.238
1.196HisArg: 1.196 ± 0.294
0.822HisSer: 0.822 ± 0.304
1.046HisThr: 1.046 ± 0.337
1.42HisVal: 1.42 ± 0.35
0.149HisTrp: 0.149 ± 0.107
1.196HisTyr: 1.196 ± 0.27
0.0HisXaa: 0.0 ± 0.0
Ile
6.053IleAla: 6.053 ± 0.624
1.196IleCys: 1.196 ± 0.32
5.007IleAsp: 5.007 ± 0.594
4.409IleGlu: 4.409 ± 0.455
2.092IlePhe: 2.092 ± 0.396
4.857IleGly: 4.857 ± 0.56
1.121IleHis: 1.121 ± 0.233
4.409IleIle: 4.409 ± 0.581
5.605IleLys: 5.605 ± 0.696
3.213IleLeu: 3.213 ± 0.445
1.793IleMet: 1.793 ± 0.351
3.288IleAsn: 3.288 ± 0.639
2.466IlePro: 2.466 ± 0.515
2.84IleGln: 2.84 ± 0.608
3.064IleArg: 3.064 ± 0.53
4.259IleSer: 4.259 ± 0.622
4.558IleThr: 4.558 ± 0.575
4.185IleVal: 4.185 ± 0.579
1.046IleTrp: 1.046 ± 0.313
2.391IleTyr: 2.391 ± 0.47
0.0IleXaa: 0.0 ± 0.0
Lys
6.95LysAla: 6.95 ± 1.068
1.046LysCys: 1.046 ± 0.288
4.558LysAsp: 4.558 ± 0.622
6.427LysGlu: 6.427 ± 0.624
2.914LysPhe: 2.914 ± 0.382
4.334LysGly: 4.334 ± 0.628
1.196LysHis: 1.196 ± 0.313
4.035LysIle: 4.035 ± 0.479
4.558LysLys: 4.558 ± 0.592
5.306LysLeu: 5.306 ± 0.601
3.587LysMet: 3.587 ± 0.509
3.139LysAsn: 3.139 ± 0.679
3.886LysPro: 3.886 ± 0.552
3.587LysGln: 3.587 ± 0.65
4.558LysArg: 4.558 ± 0.744
3.512LysSer: 3.512 ± 0.473
4.484LysThr: 4.484 ± 0.501
5.007LysVal: 5.007 ± 0.499
0.897LysTrp: 0.897 ± 0.301
1.793LysTyr: 1.793 ± 0.457
0.0LysXaa: 0.0 ± 0.0
Leu
5.829LeuAla: 5.829 ± 0.725
0.822LeuCys: 0.822 ± 0.279
3.512LeuAsp: 3.512 ± 0.642
4.334LeuGlu: 4.334 ± 0.755
1.943LeuPhe: 1.943 ± 0.373
3.811LeuGly: 3.811 ± 0.708
1.27LeuHis: 1.27 ± 0.321
5.156LeuIle: 5.156 ± 0.585
5.754LeuLys: 5.754 ± 0.64
3.736LeuLeu: 3.736 ± 0.5
1.868LeuMet: 1.868 ± 0.339
3.437LeuAsn: 3.437 ± 0.486
3.139LeuPro: 3.139 ± 0.469
1.719LeuGln: 1.719 ± 0.392
3.512LeuArg: 3.512 ± 0.449
4.409LeuSer: 4.409 ± 0.581
4.334LeuThr: 4.334 ± 0.616
3.587LeuVal: 3.587 ± 0.325
0.971LeuTrp: 0.971 ± 0.264
2.317LeuTyr: 2.317 ± 0.322
0.0LeuXaa: 0.0 ± 0.0
Met
3.886MetAla: 3.886 ± 0.603
0.374MetCys: 0.374 ± 0.157
1.495MetAsp: 1.495 ± 0.341
1.644MetGlu: 1.644 ± 0.481
0.598MetPhe: 0.598 ± 0.238
0.971MetGly: 0.971 ± 0.292
0.673MetHis: 0.673 ± 0.258
2.989MetIle: 2.989 ± 0.457
2.989MetLys: 2.989 ± 0.437
2.541MetLeu: 2.541 ± 0.524
1.196MetMet: 1.196 ± 0.293
1.345MetAsn: 1.345 ± 0.353
1.27MetPro: 1.27 ± 0.316
1.196MetGln: 1.196 ± 0.254
2.018MetArg: 2.018 ± 0.289
2.167MetSer: 2.167 ± 0.366
1.793MetThr: 1.793 ± 0.346
1.943MetVal: 1.943 ± 0.387
0.149MetTrp: 0.149 ± 0.116
1.046MetTyr: 1.046 ± 0.237
0.0MetXaa: 0.0 ± 0.0
Asn
3.736AsnAla: 3.736 ± 0.622
0.598AsnCys: 0.598 ± 0.284
3.213AsnAsp: 3.213 ± 0.506
3.363AsnGlu: 3.363 ± 0.537
1.644AsnPhe: 1.644 ± 0.311
5.38AsnGly: 5.38 ± 0.651
0.747AsnHis: 0.747 ± 0.261
3.064AsnIle: 3.064 ± 0.495
3.213AsnLys: 3.213 ± 0.45
2.242AsnLeu: 2.242 ± 0.449
0.897AsnMet: 0.897 ± 0.262
2.765AsnAsn: 2.765 ± 0.424
1.495AsnPro: 1.495 ± 0.36
1.868AsnGln: 1.868 ± 0.345
1.644AsnArg: 1.644 ± 0.358
2.242AsnSer: 2.242 ± 0.455
1.27AsnThr: 1.27 ± 0.245
3.512AsnVal: 3.512 ± 0.432
0.747AsnTrp: 0.747 ± 0.194
1.943AsnTyr: 1.943 ± 0.427
0.0AsnXaa: 0.0 ± 0.0
Pro
2.317ProAla: 2.317 ± 0.479
0.523ProCys: 0.523 ± 0.23
2.615ProAsp: 2.615 ± 0.55
3.288ProGlu: 3.288 ± 0.686
1.569ProPhe: 1.569 ± 0.311
2.765ProGly: 2.765 ± 0.42
0.448ProHis: 0.448 ± 0.16
1.943ProIle: 1.943 ± 0.487
1.42ProLys: 1.42 ± 0.289
1.719ProLeu: 1.719 ± 0.389
0.971ProMet: 0.971 ± 0.305
1.495ProAsn: 1.495 ± 0.369
1.345ProPro: 1.345 ± 0.363
1.196ProGln: 1.196 ± 0.32
1.868ProArg: 1.868 ± 0.383
1.495ProSer: 1.495 ± 0.317
1.793ProThr: 1.793 ± 0.347
2.317ProVal: 2.317 ± 0.444
0.598ProTrp: 0.598 ± 0.219
1.644ProTyr: 1.644 ± 0.369
0.0ProXaa: 0.0 ± 0.0
Gln
3.064GlnAla: 3.064 ± 0.546
0.747GlnCys: 0.747 ± 0.211
1.719GlnAsp: 1.719 ± 0.298
2.242GlnGlu: 2.242 ± 0.385
1.196GlnPhe: 1.196 ± 0.318
2.242GlnGly: 2.242 ± 0.352
0.822GlnHis: 0.822 ± 0.25
2.84GlnIle: 2.84 ± 0.573
2.391GlnLys: 2.391 ± 0.49
2.914GlnLeu: 2.914 ± 0.583
0.897GlnMet: 0.897 ± 0.32
1.046GlnAsn: 1.046 ± 0.289
1.569GlnPro: 1.569 ± 0.497
2.69GlnGln: 2.69 ± 0.725
2.391GlnArg: 2.391 ± 0.408
2.84GlnSer: 2.84 ± 0.435
1.569GlnThr: 1.569 ± 0.312
2.989GlnVal: 2.989 ± 0.514
0.523GlnTrp: 0.523 ± 0.21
1.868GlnTyr: 1.868 ± 0.397
0.0GlnXaa: 0.0 ± 0.0
Arg
4.259ArgAla: 4.259 ± 0.636
0.897ArgCys: 0.897 ± 0.28
2.242ArgAsp: 2.242 ± 0.493
3.437ArgGlu: 3.437 ± 0.437
1.644ArgPhe: 1.644 ± 0.338
2.765ArgGly: 2.765 ± 0.369
0.598ArgHis: 0.598 ± 0.236
4.259ArgIle: 4.259 ± 0.432
4.484ArgLys: 4.484 ± 0.579
2.84ArgLeu: 2.84 ± 0.519
1.644ArgMet: 1.644 ± 0.365
2.242ArgAsn: 2.242 ± 0.514
1.793ArgPro: 1.793 ± 0.427
1.793ArgGln: 1.793 ± 0.461
3.288ArgArg: 3.288 ± 0.598
2.615ArgSer: 2.615 ± 0.459
1.644ArgThr: 1.644 ± 0.38
4.334ArgVal: 4.334 ± 0.61
0.673ArgTrp: 0.673 ± 0.234
2.84ArgTyr: 2.84 ± 0.457
0.0ArgXaa: 0.0 ± 0.0
Ser
5.306SerAla: 5.306 ± 0.707
0.747SerCys: 0.747 ± 0.234
3.512SerAsp: 3.512 ± 0.469
4.708SerGlu: 4.708 ± 0.58
2.84SerPhe: 2.84 ± 0.401
5.455SerGly: 5.455 ± 0.508
1.196SerHis: 1.196 ± 0.287
4.558SerIle: 4.558 ± 0.661
4.334SerLys: 4.334 ± 0.609
3.961SerLeu: 3.961 ± 0.472
0.897SerMet: 0.897 ± 0.21
2.092SerAsn: 2.092 ± 0.506
1.943SerPro: 1.943 ± 0.352
2.167SerGln: 2.167 ± 0.395
3.064SerArg: 3.064 ± 0.468
3.587SerSer: 3.587 ± 0.668
2.765SerThr: 2.765 ± 0.523
3.886SerVal: 3.886 ± 0.483
0.971SerTrp: 0.971 ± 0.331
2.615SerTyr: 2.615 ± 0.453
0.0SerXaa: 0.0 ± 0.0
Thr
4.558ThrAla: 4.558 ± 0.82
0.523ThrCys: 0.523 ± 0.24
2.765ThrAsp: 2.765 ± 0.513
2.989ThrGlu: 2.989 ± 0.625
2.541ThrPhe: 2.541 ± 0.39
6.352ThrGly: 6.352 ± 0.762
0.448ThrHis: 0.448 ± 0.186
3.139ThrIle: 3.139 ± 0.507
3.363ThrLys: 3.363 ± 0.637
4.259ThrLeu: 4.259 ± 0.56
1.345ThrMet: 1.345 ± 0.281
3.064ThrAsn: 3.064 ± 0.518
2.018ThrPro: 2.018 ± 0.395
2.317ThrGln: 2.317 ± 0.335
1.644ThrArg: 1.644 ± 0.397
3.961ThrSer: 3.961 ± 0.561
2.018ThrThr: 2.018 ± 0.391
3.662ThrVal: 3.662 ± 0.489
0.897ThrTrp: 0.897 ± 0.24
1.868ThrTyr: 1.868 ± 0.428
0.0ThrXaa: 0.0 ± 0.0
Val
4.334ValAla: 4.334 ± 0.714
1.196ValCys: 1.196 ± 0.303
5.306ValAsp: 5.306 ± 0.537
5.007ValGlu: 5.007 ± 0.622
2.84ValPhe: 2.84 ± 0.472
3.736ValGly: 3.736 ± 0.599
1.569ValHis: 1.569 ± 0.408
4.857ValIle: 4.857 ± 0.529
5.829ValLys: 5.829 ± 0.718
4.259ValLeu: 4.259 ± 0.476
2.092ValMet: 2.092 ± 0.366
3.288ValAsn: 3.288 ± 0.431
1.868ValPro: 1.868 ± 0.579
1.943ValGln: 1.943 ± 0.385
3.288ValArg: 3.288 ± 0.495
4.558ValSer: 4.558 ± 0.611
4.857ValThr: 4.857 ± 0.668
4.185ValVal: 4.185 ± 0.512
0.822ValTrp: 0.822 ± 0.216
1.569ValTyr: 1.569 ± 0.316
0.0ValXaa: 0.0 ± 0.0
Trp
0.897TrpAla: 0.897 ± 0.296
0.075TrpCys: 0.075 ± 0.083
0.897TrpAsp: 0.897 ± 0.225
0.747TrpGlu: 0.747 ± 0.225
0.598TrpPhe: 0.598 ± 0.269
1.196TrpGly: 1.196 ± 0.306
0.822TrpHis: 0.822 ± 0.24
1.046TrpIle: 1.046 ± 0.245
0.822TrpLys: 0.822 ± 0.247
1.196TrpLeu: 1.196 ± 0.321
0.374TrpMet: 0.374 ± 0.145
0.673TrpAsn: 0.673 ± 0.221
0.299TrpPro: 0.299 ± 0.172
0.374TrpGln: 0.374 ± 0.166
1.27TrpArg: 1.27 ± 0.255
0.897TrpSer: 0.897 ± 0.247
0.673TrpThr: 0.673 ± 0.229
0.523TrpVal: 0.523 ± 0.177
0.149TrpTrp: 0.149 ± 0.106
0.598TrpTyr: 0.598 ± 0.169
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.765TyrAla: 2.765 ± 0.495
0.523TyrCys: 0.523 ± 0.186
2.615TyrAsp: 2.615 ± 0.448
2.69TyrGlu: 2.69 ± 0.485
1.27TyrPhe: 1.27 ± 0.343
2.914TyrGly: 2.914 ± 0.469
1.121TyrHis: 1.121 ± 0.259
2.018TyrIle: 2.018 ± 0.428
2.541TyrLys: 2.541 ± 0.515
2.092TyrLeu: 2.092 ± 0.359
1.42TyrMet: 1.42 ± 0.337
1.644TyrAsn: 1.644 ± 0.308
1.569TyrPro: 1.569 ± 0.365
1.943TyrGln: 1.943 ± 0.324
2.167TyrArg: 2.167 ± 0.388
2.615TyrSer: 2.615 ± 0.453
2.391TyrThr: 2.391 ± 0.338
2.317TyrVal: 2.317 ± 0.406
0.224TyrTrp: 0.224 ± 0.124
0.971TyrTyr: 0.971 ± 0.264
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 71 proteins (13383 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski