Flavobacterium phage FPSV-D35

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Unahavirus; unclassified Unahavirus

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M2VC80|A0A6M2VC80_9CAUD Uncharacterized protein OS=Flavobacterium phage FPSV-D35 OX=2575342 PE=4 SV=1
MM1 pKa = 8.06RR2 pKa = 11.84NRR4 pKa = 11.84IYY6 pKa = 10.25IQYY9 pKa = 9.89SGKK12 pKa = 8.17ITKK15 pKa = 8.21NTIALMEE22 pKa = 4.56TYY24 pKa = 10.11LICDD28 pKa = 4.78FIGRR32 pKa = 11.84TVIDD36 pKa = 3.57GRR38 pKa = 11.84RR39 pKa = 11.84AYY41 pKa = 9.61IDD43 pKa = 3.9LKK45 pKa = 10.97QSEE48 pKa = 4.73NPNLDD53 pKa = 3.37EE54 pKa = 4.11VSQFILALDD63 pKa = 3.84GTDD66 pKa = 6.66FITFWGYY73 pKa = 9.86PLEE76 pKa = 4.99DD77 pKa = 3.88GSNITTNEE85 pKa = 3.77DD86 pKa = 3.29FKK88 pKa = 11.48DD89 pKa = 4.53FSISEE94 pKa = 3.79IDD96 pKa = 3.12AA97 pKa = 4.2

Molecular weight: 11.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M2VDI7|A0A6M2VDI7_9CAUD SNF2_N domain-containing protein OS=Flavobacterium phage FPSV-D35 OX=2575342 PE=4 SV=1
MM1 pKa = 7.77HH2 pKa = 6.82IQILIMQQQPTIKK15 pKa = 10.4LRR17 pKa = 11.84DD18 pKa = 4.03AIKK21 pKa = 10.7KK22 pKa = 8.45MRR24 pKa = 11.84ALTGINVPFSFSYY37 pKa = 10.2ISYY40 pKa = 10.62SEE42 pKa = 4.16VLNKK46 pKa = 10.35SEE48 pKa = 4.35GYY50 pKa = 10.05KK51 pKa = 10.08RR52 pKa = 11.84VNKK55 pKa = 10.1GLLRR59 pKa = 11.84AGYY62 pKa = 9.35RR63 pKa = 11.84ANQSEE68 pKa = 4.24RR69 pKa = 11.84SNILIAYY76 pKa = 8.87IDD78 pKa = 4.03YY79 pKa = 10.62DD80 pKa = 3.54KK81 pKa = 11.66KK82 pKa = 10.39EE83 pKa = 4.11ADD85 pKa = 3.69RR86 pKa = 11.84QFYY89 pKa = 10.85LPLLMTFNNIQVIPP103 pKa = 3.92

Molecular weight: 12.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12785

65

1464

232.5

26.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.632 ± 0.521

0.72 ± 0.094

5.616 ± 0.18

7.415 ± 0.241

4.889 ± 0.303

5.061 ± 0.272

1.572 ± 0.167

8.69 ± 0.275

9.542 ± 0.399

9.026 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.315 ± 0.149

6.86 ± 0.305

2.855 ± 0.194

3.723 ± 0.166

3.747 ± 0.183

6.226 ± 0.226

5.209 ± 0.286

5.585 ± 0.219

1.197 ± 0.144

4.122 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski