Amino acid dipepetide frequency for Flavobacterium phage FPSV-D35

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.581AlaAla: 2.581 ± 0.853
0.313AlaCys: 0.313 ± 0.165
3.051AlaAsp: 3.051 ± 0.618
3.911AlaGlu: 3.911 ± 0.664
3.442AlaPhe: 3.442 ± 0.452
3.833AlaGly: 3.833 ± 0.793
0.704AlaHis: 0.704 ± 0.207
5.632AlaIle: 5.632 ± 0.749
4.772AlaLys: 4.772 ± 1.425
5.163AlaLeu: 5.163 ± 0.87
1.017AlaMet: 1.017 ± 0.222
3.989AlaAsn: 3.989 ± 0.633
0.86AlaPro: 0.86 ± 0.215
2.19AlaGln: 2.19 ± 0.779
1.721AlaArg: 1.721 ± 0.296
3.598AlaSer: 3.598 ± 0.722
3.285AlaThr: 3.285 ± 0.612
2.972AlaVal: 2.972 ± 0.485
0.704AlaTrp: 0.704 ± 0.232
2.581AlaTyr: 2.581 ± 0.387
0.0AlaXaa: 0.0 ± 0.0
Cys
0.313CysAla: 0.313 ± 0.149
0.235CysCys: 0.235 ± 0.145
0.86CysAsp: 0.86 ± 0.272
0.313CysGlu: 0.313 ± 0.21
0.313CysPhe: 0.313 ± 0.142
0.235CysGly: 0.235 ± 0.124
0.235CysHis: 0.235 ± 0.136
0.156CysIle: 0.156 ± 0.11
0.626CysLys: 0.626 ± 0.299
0.469CysLeu: 0.469 ± 0.203
0.235CysMet: 0.235 ± 0.137
0.391CysAsn: 0.391 ± 0.198
0.391CysPro: 0.391 ± 0.192
0.313CysGln: 0.313 ± 0.169
0.235CysArg: 0.235 ± 0.184
0.391CysSer: 0.391 ± 0.156
0.548CysThr: 0.548 ± 0.206
0.469CysVal: 0.469 ± 0.171
0.0CysTrp: 0.0 ± 0.0
0.469CysTyr: 0.469 ± 0.217
0.0CysXaa: 0.0 ± 0.0
Asp
3.833AspAla: 3.833 ± 0.648
0.626AspCys: 0.626 ± 0.237
2.972AspAsp: 2.972 ± 0.51
4.615AspGlu: 4.615 ± 0.602
3.051AspPhe: 3.051 ± 0.503
3.129AspGly: 3.129 ± 0.518
0.626AspHis: 0.626 ± 0.264
4.772AspIle: 4.772 ± 0.549
5.084AspLys: 5.084 ± 0.553
5.163AspLeu: 5.163 ± 0.772
0.86AspMet: 0.86 ± 0.218
2.816AspAsn: 2.816 ± 0.377
1.017AspPro: 1.017 ± 0.244
1.173AspGln: 1.173 ± 0.276
2.112AspArg: 2.112 ± 0.339
4.068AspSer: 4.068 ± 0.695
2.425AspThr: 2.425 ± 0.539
3.52AspVal: 3.52 ± 0.508
0.782AspTrp: 0.782 ± 0.234
3.52AspTyr: 3.52 ± 0.622
0.0AspXaa: 0.0 ± 0.0
Glu
4.068GluAla: 4.068 ± 0.527
0.704GluCys: 0.704 ± 0.213
3.755GluAsp: 3.755 ± 0.579
4.693GluGlu: 4.693 ± 0.547
3.051GluPhe: 3.051 ± 0.495
3.911GluGly: 3.911 ± 0.509
1.017GluHis: 1.017 ± 0.297
8.213GluIle: 8.213 ± 1.015
6.805GluLys: 6.805 ± 0.625
8.135GluLeu: 8.135 ± 1.065
1.408GluMet: 1.408 ± 0.274
5.788GluAsn: 5.788 ± 0.764
1.252GluPro: 1.252 ± 0.416
2.66GluGln: 2.66 ± 0.446
2.894GluArg: 2.894 ± 0.445
3.755GluSer: 3.755 ± 0.472
3.207GluThr: 3.207 ± 0.474
4.85GluVal: 4.85 ± 0.717
0.86GluTrp: 0.86 ± 0.288
3.051GluTyr: 3.051 ± 0.584
0.0GluXaa: 0.0 ± 0.0
Phe
1.956PheAla: 1.956 ± 0.375
0.548PheCys: 0.548 ± 0.143
3.285PheAsp: 3.285 ± 0.502
5.163PheGlu: 5.163 ± 0.806
1.721PhePhe: 1.721 ± 0.408
2.503PheGly: 2.503 ± 0.516
0.782PheHis: 0.782 ± 0.277
3.989PheIle: 3.989 ± 0.591
3.364PheLys: 3.364 ± 0.464
4.146PheLeu: 4.146 ± 0.726
0.86PheMet: 0.86 ± 0.305
2.268PheAsn: 2.268 ± 0.382
1.564PhePro: 1.564 ± 0.353
1.799PheGln: 1.799 ± 0.455
2.816PheArg: 2.816 ± 0.476
3.676PheSer: 3.676 ± 0.539
2.19PheThr: 2.19 ± 0.354
2.738PheVal: 2.738 ± 0.525
1.173PheTrp: 1.173 ± 0.286
2.347PheTyr: 2.347 ± 0.505
0.0PheXaa: 0.0 ± 0.0
Gly
2.738GlyAla: 2.738 ± 0.646
0.156GlyCys: 0.156 ± 0.098
2.268GlyAsp: 2.268 ± 0.417
2.894GlyGlu: 2.894 ± 0.558
2.972GlyPhe: 2.972 ± 0.484
2.581GlyGly: 2.581 ± 0.456
0.548GlyHis: 0.548 ± 0.239
4.85GlyIle: 4.85 ± 0.532
4.146GlyLys: 4.146 ± 0.83
4.537GlyLeu: 4.537 ± 0.654
1.643GlyMet: 1.643 ± 0.404
3.442GlyAsn: 3.442 ± 0.46
0.86GlyPro: 0.86 ± 0.223
1.33GlyGln: 1.33 ± 0.269
1.877GlyArg: 1.877 ± 0.447
3.051GlySer: 3.051 ± 0.55
3.285GlyThr: 3.285 ± 0.397
3.676GlyVal: 3.676 ± 0.677
0.939GlyTrp: 0.939 ± 0.222
2.816GlyTyr: 2.816 ± 0.437
0.0GlyXaa: 0.0 ± 0.0
His
0.548HisAla: 0.548 ± 0.206
0.078HisCys: 0.078 ± 0.083
0.626HisAsp: 0.626 ± 0.201
1.017HisGlu: 1.017 ± 0.309
1.017HisPhe: 1.017 ± 0.342
0.86HisGly: 0.86 ± 0.206
0.078HisHis: 0.078 ± 0.082
1.721HisIle: 1.721 ± 0.419
1.643HisLys: 1.643 ± 0.499
1.877HisLeu: 1.877 ± 0.369
0.313HisMet: 0.313 ± 0.112
1.095HisAsn: 1.095 ± 0.226
0.469HisPro: 0.469 ± 0.209
0.548HisGln: 0.548 ± 0.169
0.86HisArg: 0.86 ± 0.228
0.86HisSer: 0.86 ± 0.286
0.782HisThr: 0.782 ± 0.172
0.469HisVal: 0.469 ± 0.18
0.078HisTrp: 0.078 ± 0.084
0.782HisTyr: 0.782 ± 0.223
0.0HisXaa: 0.0 ± 0.0
Ile
5.71IleAla: 5.71 ± 0.918
0.626IleCys: 0.626 ± 0.252
5.319IleAsp: 5.319 ± 0.642
8.839IleGlu: 8.839 ± 0.721
4.302IlePhe: 4.302 ± 0.552
2.738IleGly: 2.738 ± 0.39
2.034IleHis: 2.034 ± 0.458
5.554IleIle: 5.554 ± 0.606
8.996IleLys: 8.996 ± 0.643
6.492IleLeu: 6.492 ± 0.642
2.347IleMet: 2.347 ± 0.46
6.101IleAsn: 6.101 ± 0.67
2.894IlePro: 2.894 ± 0.4
3.833IleGln: 3.833 ± 0.479
3.207IleArg: 3.207 ± 0.575
5.945IleSer: 5.945 ± 0.553
4.068IleThr: 4.068 ± 0.515
4.615IleVal: 4.615 ± 0.663
0.469IleTrp: 0.469 ± 0.218
2.816IleTyr: 2.816 ± 0.508
0.0IleXaa: 0.0 ± 0.0
Lys
5.788LysAla: 5.788 ± 0.844
0.391LysCys: 0.391 ± 0.198
4.146LysAsp: 4.146 ± 0.398
7.744LysGlu: 7.744 ± 0.822
3.52LysPhe: 3.52 ± 0.48
3.755LysGly: 3.755 ± 0.577
1.252LysHis: 1.252 ± 0.302
9.074LysIle: 9.074 ± 0.885
9.309LysLys: 9.309 ± 1.244
8.37LysLeu: 8.37 ± 1.018
2.347LysMet: 2.347 ± 0.431
7.118LysAsn: 7.118 ± 0.728
2.347LysPro: 2.347 ± 0.383
3.364LysGln: 3.364 ± 0.722
4.146LysArg: 4.146 ± 0.602
5.554LysSer: 5.554 ± 0.532
4.928LysThr: 4.928 ± 0.685
6.023LysVal: 6.023 ± 0.514
1.33LysTrp: 1.33 ± 0.297
4.928LysTyr: 4.928 ± 0.637
0.0LysXaa: 0.0 ± 0.0
Leu
5.397LeuAla: 5.397 ± 0.556
0.704LeuCys: 0.704 ± 0.26
4.38LeuAsp: 4.38 ± 0.574
6.023LeuGlu: 6.023 ± 0.604
4.224LeuPhe: 4.224 ± 0.695
4.302LeuGly: 4.302 ± 0.63
1.33LeuHis: 1.33 ± 0.322
6.727LeuIle: 6.727 ± 0.791
9.621LeuLys: 9.621 ± 0.9
8.448LeuLeu: 8.448 ± 0.716
1.877LeuMet: 1.877 ± 0.374
6.492LeuAsn: 6.492 ± 0.801
3.755LeuPro: 3.755 ± 0.679
4.224LeuGln: 4.224 ± 0.515
3.442LeuArg: 3.442 ± 0.586
6.336LeuSer: 6.336 ± 0.713
5.084LeuThr: 5.084 ± 0.654
3.989LeuVal: 3.989 ± 0.434
1.017LeuTrp: 1.017 ± 0.242
2.894LeuTyr: 2.894 ± 0.396
0.0LeuXaa: 0.0 ± 0.0
Met
1.877MetAla: 1.877 ± 0.418
0.0MetCys: 0.0 ± 0.0
1.486MetAsp: 1.486 ± 0.305
1.33MetGlu: 1.33 ± 0.351
0.782MetPhe: 0.782 ± 0.236
0.704MetGly: 0.704 ± 0.218
0.235MetHis: 0.235 ± 0.149
1.173MetIle: 1.173 ± 0.233
2.972MetLys: 2.972 ± 0.487
1.956MetLeu: 1.956 ± 0.533
0.86MetMet: 0.86 ± 0.32
1.643MetAsn: 1.643 ± 0.316
0.548MetPro: 0.548 ± 0.198
1.173MetGln: 1.173 ± 0.325
0.782MetArg: 0.782 ± 0.212
1.33MetSer: 1.33 ± 0.267
1.956MetThr: 1.956 ± 0.333
1.095MetVal: 1.095 ± 0.42
0.235MetTrp: 0.235 ± 0.124
1.017MetTyr: 1.017 ± 0.358
0.0MetXaa: 0.0 ± 0.0
Asn
3.676AsnAla: 3.676 ± 0.55
0.469AsnCys: 0.469 ± 0.209
3.911AsnAsp: 3.911 ± 0.542
4.772AsnGlu: 4.772 ± 0.483
3.442AsnPhe: 3.442 ± 0.686
4.224AsnGly: 4.224 ± 0.491
1.173AsnHis: 1.173 ± 0.257
5.71AsnIle: 5.71 ± 0.741
6.414AsnLys: 6.414 ± 0.648
5.632AsnLeu: 5.632 ± 0.629
1.095AsnMet: 1.095 ± 0.33
4.459AsnAsn: 4.459 ± 0.602
1.956AsnPro: 1.956 ± 0.382
2.347AsnGln: 2.347 ± 0.404
3.207AsnArg: 3.207 ± 0.604
4.459AsnSer: 4.459 ± 0.651
4.146AsnThr: 4.146 ± 0.687
4.068AsnVal: 4.068 ± 0.505
0.548AsnTrp: 0.548 ± 0.182
2.894AsnTyr: 2.894 ± 0.514
0.0AsnXaa: 0.0 ± 0.0
Pro
1.33ProAla: 1.33 ± 0.353
0.235ProCys: 0.235 ± 0.139
1.252ProAsp: 1.252 ± 0.251
2.66ProGlu: 2.66 ± 0.485
1.721ProPhe: 1.721 ± 0.453
1.173ProGly: 1.173 ± 0.279
0.235ProHis: 0.235 ± 0.125
2.268ProIle: 2.268 ± 0.362
2.347ProLys: 2.347 ± 0.504
2.503ProLeu: 2.503 ± 0.427
0.469ProMet: 0.469 ± 0.21
2.581ProAsn: 2.581 ± 0.312
0.782ProPro: 0.782 ± 0.253
1.017ProGln: 1.017 ± 0.264
0.704ProArg: 0.704 ± 0.23
1.956ProSer: 1.956 ± 0.401
1.408ProThr: 1.408 ± 0.367
2.503ProVal: 2.503 ± 0.624
0.313ProTrp: 0.313 ± 0.122
1.095ProTyr: 1.095 ± 0.308
0.0ProXaa: 0.0 ± 0.0
Gln
2.19GlnAla: 2.19 ± 0.644
0.235GlnCys: 0.235 ± 0.122
1.877GlnAsp: 1.877 ± 0.325
3.442GlnGlu: 3.442 ± 0.544
1.017GlnPhe: 1.017 ± 0.32
1.643GlnGly: 1.643 ± 0.309
0.626GlnHis: 0.626 ± 0.199
3.364GlnIle: 3.364 ± 0.534
3.989GlnLys: 3.989 ± 0.613
3.755GlnLeu: 3.755 ± 0.433
1.095GlnMet: 1.095 ± 0.23
2.19GlnAsn: 2.19 ± 0.37
1.017GlnPro: 1.017 ± 0.261
1.721GlnGln: 1.721 ± 0.405
1.564GlnArg: 1.564 ± 0.379
1.721GlnSer: 1.721 ± 0.32
2.347GlnThr: 2.347 ± 0.398
1.643GlnVal: 1.643 ± 0.37
0.548GlnTrp: 0.548 ± 0.201
1.252GlnTyr: 1.252 ± 0.279
0.0GlnXaa: 0.0 ± 0.0
Arg
2.425ArgAla: 2.425 ± 0.354
0.626ArgCys: 0.626 ± 0.229
2.738ArgAsp: 2.738 ± 0.505
2.972ArgGlu: 2.972 ± 0.496
2.19ArgPhe: 2.19 ± 0.502
1.799ArgGly: 1.799 ± 0.325
0.86ArgHis: 0.86 ± 0.338
3.911ArgIle: 3.911 ± 0.475
3.989ArgLys: 3.989 ± 0.509
3.129ArgLeu: 3.129 ± 0.421
0.782ArgMet: 0.782 ± 0.228
2.347ArgAsn: 2.347 ± 0.403
0.704ArgPro: 0.704 ± 0.211
1.33ArgGln: 1.33 ± 0.28
1.095ArgArg: 1.095 ± 0.376
1.721ArgSer: 1.721 ± 0.36
1.643ArgThr: 1.643 ± 0.298
2.425ArgVal: 2.425 ± 0.471
0.078ArgTrp: 0.078 ± 0.078
0.704ArgTyr: 0.704 ± 0.229
0.0ArgXaa: 0.0 ± 0.0
Ser
3.598SerAla: 3.598 ± 0.446
0.391SerCys: 0.391 ± 0.151
4.38SerAsp: 4.38 ± 0.499
3.833SerGlu: 3.833 ± 0.59
4.38SerPhe: 4.38 ± 0.583
3.755SerGly: 3.755 ± 0.7
0.86SerHis: 0.86 ± 0.244
5.319SerIle: 5.319 ± 0.595
5.788SerLys: 5.788 ± 0.784
5.084SerLeu: 5.084 ± 0.499
1.877SerMet: 1.877 ± 0.529
4.146SerAsn: 4.146 ± 0.484
2.034SerPro: 2.034 ± 0.363
2.425SerGln: 2.425 ± 0.435
1.564SerArg: 1.564 ± 0.35
3.755SerSer: 3.755 ± 0.479
3.285SerThr: 3.285 ± 0.511
3.129SerVal: 3.129 ± 0.434
0.626SerTrp: 0.626 ± 0.209
2.034SerTyr: 2.034 ± 0.394
0.0SerXaa: 0.0 ± 0.0
Thr
2.738ThrAla: 2.738 ± 0.777
0.235ThrCys: 0.235 ± 0.137
3.364ThrAsp: 3.364 ± 0.549
3.442ThrGlu: 3.442 ± 0.473
2.503ThrPhe: 2.503 ± 0.441
4.38ThrGly: 4.38 ± 0.638
0.782ThrHis: 0.782 ± 0.235
5.084ThrIle: 5.084 ± 0.64
5.006ThrLys: 5.006 ± 0.659
4.459ThrLeu: 4.459 ± 0.524
1.017ThrMet: 1.017 ± 0.234
3.442ThrAsn: 3.442 ± 0.552
2.581ThrPro: 2.581 ± 0.416
1.799ThrGln: 1.799 ± 0.362
1.408ThrArg: 1.408 ± 0.362
2.581ThrSer: 2.581 ± 0.456
2.347ThrThr: 2.347 ± 0.436
2.581ThrVal: 2.581 ± 0.373
0.391ThrTrp: 0.391 ± 0.136
1.956ThrTyr: 1.956 ± 0.544
0.0ThrXaa: 0.0 ± 0.0
Val
3.676ValAla: 3.676 ± 0.839
0.391ValCys: 0.391 ± 0.172
3.598ValAsp: 3.598 ± 0.45
3.911ValGlu: 3.911 ± 0.384
2.738ValPhe: 2.738 ± 0.541
2.503ValGly: 2.503 ± 0.408
1.486ValHis: 1.486 ± 0.403
4.537ValIle: 4.537 ± 0.473
3.989ValLys: 3.989 ± 0.597
5.241ValLeu: 5.241 ± 0.504
1.799ValMet: 1.799 ± 0.499
4.068ValAsn: 4.068 ± 0.556
1.643ValPro: 1.643 ± 0.511
1.799ValGln: 1.799 ± 0.452
1.564ValArg: 1.564 ± 0.263
4.068ValSer: 4.068 ± 0.514
2.347ValThr: 2.347 ± 0.405
3.285ValVal: 3.285 ± 0.468
1.173ValTrp: 1.173 ± 0.298
2.034ValTyr: 2.034 ± 0.481
0.0ValXaa: 0.0 ± 0.0
Trp
0.704TrpAla: 0.704 ± 0.2
0.078TrpCys: 0.078 ± 0.078
0.939TrpAsp: 0.939 ± 0.347
0.548TrpGlu: 0.548 ± 0.205
0.313TrpPhe: 0.313 ± 0.114
0.626TrpGly: 0.626 ± 0.232
0.156TrpHis: 0.156 ± 0.096
0.782TrpIle: 0.782 ± 0.296
1.486TrpLys: 1.486 ± 0.357
1.877TrpLeu: 1.877 ± 0.429
0.235TrpMet: 0.235 ± 0.118
0.469TrpAsn: 0.469 ± 0.195
0.235TrpPro: 0.235 ± 0.13
0.548TrpGln: 0.548 ± 0.188
0.313TrpArg: 0.313 ± 0.139
0.391TrpSer: 0.391 ± 0.185
0.548TrpThr: 0.548 ± 0.143
0.86TrpVal: 0.86 ± 0.281
0.156TrpTrp: 0.156 ± 0.091
0.704TrpTyr: 0.704 ± 0.242
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.173TyrAla: 1.173 ± 0.322
0.156TyrCys: 0.156 ± 0.119
1.956TyrAsp: 1.956 ± 0.333
2.034TyrGlu: 2.034 ± 0.349
2.19TyrPhe: 2.19 ± 0.471
1.956TyrGly: 1.956 ± 0.396
0.704TyrHis: 0.704 ± 0.243
4.068TyrIle: 4.068 ± 0.538
5.084TyrLys: 5.084 ± 0.545
3.833TyrLeu: 3.833 ± 0.634
0.939TyrMet: 0.939 ± 0.26
3.755TyrAsn: 3.755 ± 0.625
1.643TyrPro: 1.643 ± 0.359
1.643TyrGln: 1.643 ± 0.331
1.956TyrArg: 1.956 ± 0.453
3.051TyrSer: 3.051 ± 0.459
2.268TyrThr: 2.268 ± 0.463
0.939TyrVal: 0.939 ± 0.338
0.548TyrTrp: 0.548 ± 0.188
1.33TyrTyr: 1.33 ± 0.343
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (12785 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski