Mycobacterium kansasii 824

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium kansasii

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7684 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X7XX58|X7XX58_MYCKA Putative ferredoxin-NADP+ reductase OS=Mycobacterium kansasii 824 OX=1299328 GN=I547_5864 PE=4 SV=1
MM1 pKa = 7.29NLASTSATSAGSPAPFLSQIADD23 pKa = 3.57NLDD26 pKa = 3.59GYY28 pKa = 10.31IQLTGQSLGAAVTDD42 pKa = 4.45FNSNLYY48 pKa = 10.07NLPQHH53 pKa = 6.7LMAAFSDD60 pKa = 4.06LLAGNISGAVQQVANGVFGLFVDD83 pKa = 4.64TSSLFSVTGNFPQLTAVVNGALGDD107 pKa = 4.05LLPILTIPGEE117 pKa = 4.25SAQNVANVVKK127 pKa = 10.67LLTDD131 pKa = 3.35PTISVDD137 pKa = 3.38VTNFLAPTQTLGFPVALGLEE157 pKa = 4.38LVGPAFSTAAAAGKK171 pKa = 9.61SAAAFSQAVQAGNMPAALTVLVDD194 pKa = 4.38APAVIADD201 pKa = 4.03GFLNGQYY208 pKa = 10.83VLPTPLTLTVPFVVPLVGVVPQTITLQNNVPFNGILHH245 pKa = 5.95PLEE248 pKa = 5.44RR249 pKa = 11.84ITAIAPNPLTGGMLNVTTNGTEE271 pKa = 3.37IGGIVPALLNYY282 pKa = 9.06WPQQLANAIGAA293 pKa = 3.98

Molecular weight: 29.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X7YBN4|X7YBN4_MYCKA TVP38/TMEM64 family membrane protein OS=Mycobacterium kansasii 824 OX=1299328 GN=I547_1551 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSGRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.66GRR42 pKa = 11.84RR43 pKa = 11.84ALTAA47 pKa = 3.88

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7684

0

7684

1907453

34

2559

248.2

26.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.231 ± 0.043

1.027 ± 0.01

5.746 ± 0.026

4.656 ± 0.026

2.978 ± 0.019

9.275 ± 0.06

2.304 ± 0.015

4.125 ± 0.022

1.976 ± 0.018

9.572 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.092 ± 0.014

2.438 ± 0.034

6.124 ± 0.032

3.102 ± 0.017

7.695 ± 0.037

5.729 ± 0.022

6.022 ± 0.021

8.222 ± 0.033

1.59 ± 0.014

2.096 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski