Squirrel monkey polyomavirus
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|A8Y987-2|VP2-2_POVSM Isoform of A8Y987 Isoform VP3 of Minor capsid protein VP2 OS=Squirrel monkey polyomavirus OX=452475 GN=VP2 PE=3 SV=1
MM1 pKa = 7.31 GALLAVLAEE10 pKa = 4.29 VVEE13 pKa = 4.39 LASVTGLSVEE23 pKa = 4.31 SFISGEE29 pKa = 3.99 AFATAEE35 pKa = 4.19 LLEE38 pKa = 3.97 AHH40 pKa = 7.15 IANLVTVGGLTEE52 pKa = 4.49 AEE54 pKa = 3.83 ALAATEE60 pKa = 4.53 VPAEE64 pKa = 4.29 AYY66 pKa = 9.12 AALTSLSSTFPQAFTAVAATEE87 pKa = 4.01 LATTGTLTVGATVAAALYY105 pKa = 8.79 PYY107 pKa = 9.81 YY108 pKa = 10.37 YY109 pKa = 10.45 DD110 pKa = 3.48 YY111 pKa = 10.56 STPVANLNRR120 pKa = 11.84 GLNPEE125 pKa = 4.22 MALQLWFPEE134 pKa = 3.59 IDD136 pKa = 3.62 YY137 pKa = 9.26 EE138 pKa = 5.16 FPGLMPFVRR147 pKa = 11.84 FINYY151 pKa = 9.15 IDD153 pKa = 3.59 PTQWATNLFEE163 pKa = 4.7 TIGRR167 pKa = 11.84 YY168 pKa = 7.68 SWEE171 pKa = 3.68 SAQRR175 pKa = 11.84 YY176 pKa = 5.49 GQNLIAHH183 pKa = 6.71 EE184 pKa = 4.3 VRR186 pKa = 11.84 SASRR190 pKa = 11.84 EE191 pKa = 3.93 LATRR195 pKa = 11.84 TAQGFSEE202 pKa = 4.24 AVARR206 pKa = 11.84 YY207 pKa = 9.01 FEE209 pKa = 4.05 NARR212 pKa = 11.84 WAVSTLPRR220 pKa = 11.84 SLYY223 pKa = 10.8 SGLQSYY229 pKa = 9.15 YY230 pKa = 10.58 EE231 pKa = 4.12 QLPSLNPMQVRR242 pKa = 11.84 DD243 pKa = 3.22 LHH245 pKa = 6.68 RR246 pKa = 11.84 RR247 pKa = 11.84 LGQPIPNRR255 pKa = 11.84 IALEE259 pKa = 3.91 EE260 pKa = 3.87 QAIKK264 pKa = 10.32 SAEE267 pKa = 4.08 YY268 pKa = 8.94 VQKK271 pKa = 10.7 VDD273 pKa = 3.87 PPGGANQRR281 pKa = 11.84 IAPDD285 pKa = 3.16 WLLPLILGLYY295 pKa = 10.37 GDD297 pKa = 5.2 ISPSWEE303 pKa = 3.98 STLEE307 pKa = 4.53 DD308 pKa = 3.47 IEE310 pKa = 4.55 EE311 pKa = 4.46 EE312 pKa = 3.8 EE313 pKa = 5.56 DD314 pKa = 3.62 APQKK318 pKa = 10.58 KK319 pKa = 9.27 KK320 pKa = 10.74 RR321 pKa = 11.84 KK322 pKa = 6.86 RR323 pKa = 11.84 TKK325 pKa = 10.5 KK326 pKa = 8.54 NTPRR330 pKa = 11.84 SAA332 pKa = 3.11
Molecular weight: 36.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.796
IPC2_protein 4.863
IPC_protein 4.762
Toseland 4.635
ProMoST 4.838
Dawson 4.685
Bjellqvist 4.825
Wikipedia 4.495
Rodwell 4.609
Grimsley 4.546
Solomon 4.673
Lehninger 4.635
Nozaki 4.787
DTASelect 4.863
Thurlkill 4.622
EMBOSS 4.52
Sillero 4.863
Patrickios 3.783
IPC_peptide 4.685
IPC2_peptide 4.863
IPC2.peptide.svr19 4.846
Protein with the highest isoelectric point:
>sp|A8Y987|VP2_POVSM Minor capsid protein VP2 OS=Squirrel monkey polyomavirus OX=452475 GN=VP2 PE=3 SV=1
MM1 pKa = 7.37 ARR3 pKa = 11.84 KK4 pKa = 9.87 LMARR8 pKa = 11.84 KK9 pKa = 7.39 TPPLVGLDD17 pKa = 3.6 TVQLRR22 pKa = 11.84 VEE24 pKa = 4.68 TIWLTSQMDD33 pKa = 4.24 LFVLTDD39 pKa = 3.25 KK40 pKa = 11.47 GEE42 pKa = 4.08 PRR44 pKa = 11.84 ITASRR49 pKa = 11.84 ASASRR54 pKa = 11.84 VATFGMTSVWLKK66 pKa = 10.57 NSCFF70 pKa = 3.6
Molecular weight: 7.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.254
IPC2_protein 9.37
IPC_protein 9.648
Toseland 10.76
ProMoST 10.262
Dawson 10.789
Bjellqvist 10.409
Wikipedia 10.935
Rodwell 11.125
Grimsley 10.804
Solomon 10.921
Lehninger 10.906
Nozaki 10.745
DTASelect 10.409
Thurlkill 10.716
EMBOSS 11.14
Sillero 10.73
Patrickios 10.95
IPC_peptide 10.935
IPC2_peptide 9.209
IPC2.peptide.svr19 8.6
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
2
8
2085
70
655
260.6
29.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.619 ± 1.376
1.871 ± 0.685
4.365 ± 0.289
7.77 ± 0.36
4.221 ± 0.671
5.228 ± 0.455
1.439 ± 0.296
4.892 ± 0.329
6.811 ± 1.067
11.079 ± 0.653
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.158 ± 0.271
4.556 ± 0.551
5.851 ± 0.85
4.748 ± 0.326
4.604 ± 1.013
6.427 ± 0.354
6.187 ± 0.489
5.659 ± 0.643
1.727 ± 0.322
3.789 ± 0.349
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here