Acidianus brierleyi

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; Acidianus

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2858 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U9IE46|A0A2U9IE46_9CREN ABC transporter ATP-binding protein OS=Acidianus brierleyi OX=41673 GN=DFR85_06280 PE=4 SV=1
MM1 pKa = 6.95VTLKK5 pKa = 11.0CPVCGGNINLEE16 pKa = 4.13DD17 pKa = 4.27DD18 pKa = 4.31ALPGEE23 pKa = 4.69IIEE26 pKa = 4.8HH27 pKa = 5.89EE28 pKa = 4.52CGAQLEE34 pKa = 4.8VYY36 pKa = 10.26NDD38 pKa = 3.48NGRR41 pKa = 11.84LALRR45 pKa = 11.84LAEE48 pKa = 4.3QVGEE52 pKa = 4.26DD53 pKa = 3.07WGEE56 pKa = 3.62

Molecular weight: 6.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U9IEK8|A0A2U9IEK8_9CREN Peptidyl-prolyl cis-trans isomerase OS=Acidianus brierleyi OX=41673 GN=DFR85_07320 PE=3 SV=1
MM1 pKa = 7.66KK2 pKa = 8.54GTPSFGKK9 pKa = 9.49MNNNYY14 pKa = 7.14THH16 pKa = 6.5IRR18 pKa = 11.84CRR20 pKa = 11.84RR21 pKa = 11.84CGRR24 pKa = 11.84NSYY27 pKa = 10.77NVTKK31 pKa = 9.77HH32 pKa = 4.91YY33 pKa = 10.59CAACGFGKK41 pKa = 9.85SKK43 pKa = 10.68RR44 pKa = 11.84IRR46 pKa = 11.84SYY48 pKa = 9.35SWQNKK53 pKa = 7.17KK54 pKa = 10.49VNGVRR59 pKa = 11.84IKK61 pKa = 10.94

Molecular weight: 7.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2858

0

2858

790434

32

1361

276.6

31.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.432 ± 0.043

0.664 ± 0.016

4.941 ± 0.046

6.666 ± 0.06

4.719 ± 0.041

6.422 ± 0.042

1.257 ± 0.016

10.491 ± 0.065

8.105 ± 0.066

9.853 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.298 ± 0.021

5.418 ± 0.036

3.803 ± 0.031

2.002 ± 0.025

3.803 ± 0.037

7.151 ± 0.038

4.679 ± 0.036

6.469 ± 0.032

1.017 ± 0.019

4.81 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski