Pseudopropionibacterium propionicum
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3001 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S4UTM1|A0A3S4UTM1_9ACTN LysM domain/BON superfamily protein OS=Pseudopropionibacterium propionicum OX=1750 GN=NCTC12967_00813 PE=4 SV=1
MM1 pKa = 7.49 ALAYY5 pKa = 10.53 LGAEE9 pKa = 4.25 PPGDD13 pKa = 3.54 LLDD16 pKa = 4.08 RR17 pKa = 11.84 LGQIEE22 pKa = 4.66 VPVLSIAGTEE32 pKa = 4.01 DD33 pKa = 3.54 ALVGCSPVRR42 pKa = 11.84 ALSDD46 pKa = 3.18 VFARR50 pKa = 11.84 GEE52 pKa = 3.95 FVAIDD57 pKa = 3.85 DD58 pKa = 4.53 CGHH61 pKa = 5.85 YY62 pKa = 10.13 PWVEE66 pKa = 3.97 QPAAFISVISGFTDD80 pKa = 3.58 SLLFWSPDD88 pKa = 3.25 EE89 pKa = 4.89 APGDD93 pKa = 3.92 EE94 pKa = 4.09 QDD96 pKa = 3.64 QGLFF100 pKa = 3.53
Molecular weight: 10.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.802
IPC2_protein 3.617
IPC_protein 3.567
Toseland 3.363
ProMoST 3.757
Dawson 3.567
Bjellqvist 3.732
Wikipedia 3.528
Rodwell 3.401
Grimsley 3.274
Solomon 3.541
Lehninger 3.49
Nozaki 3.706
DTASelect 3.91
Thurlkill 3.439
EMBOSS 3.541
Sillero 3.694
Patrickios 1.825
IPC_peptide 3.528
IPC2_peptide 3.656
IPC2.peptide.svr19 3.675
Protein with the highest isoelectric point:
>tr|A0A3N4CXZ2|A0A3N4CXZ2_9ACTN DUF349 domain-containing protein OS=Pseudopropionibacterium propionicum OX=1750 GN=FBF34_07925 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SRR15 pKa = 11.84 THH17 pKa = 6.17 GFRR20 pKa = 11.84 SRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILSARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.98 GRR40 pKa = 11.84 TEE42 pKa = 3.99 LSAA45 pKa = 4.86
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.464
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.34
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.076
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.14
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3001
0
3001
1008578
29
5084
336.1
36.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.749 ± 0.056
0.85 ± 0.013
5.668 ± 0.037
6.041 ± 0.04
3.098 ± 0.027
8.893 ± 0.038
2.106 ± 0.022
4.441 ± 0.029
2.591 ± 0.035
10.626 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.083 ± 0.02
2.356 ± 0.023
5.494 ± 0.036
2.988 ± 0.025
7.429 ± 0.051
5.677 ± 0.037
5.982 ± 0.038
8.469 ± 0.044
1.575 ± 0.018
1.883 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here