Xenorhabdus bovienii (strain SS-2004)
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4102 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D3UXU7|D3UXU7_XENBS Transposase OS=Xenorhabdus bovienii (strain SS-2004) OX=406818 GN=XBJ1_1339 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 10.51 LHH4 pKa = 7.01 FSTDD8 pKa = 3.18 NAEE11 pKa = 4.21 LPEE14 pKa = 4.52 CVLSGDD20 pKa = 5.33 DD21 pKa = 3.75 MQQMGFMPNTLFHH34 pKa = 6.25 IQQYY38 pKa = 11.16 NNGLMLTLAEE48 pKa = 4.76 PDD50 pKa = 4.29 VIPLSHH56 pKa = 6.35 TAEE59 pKa = 4.05 NDD61 pKa = 3.42 PYY63 pKa = 11.28 QGIDD67 pKa = 2.95 WVRR70 pKa = 11.84 DD71 pKa = 3.4 NGEE74 pKa = 4.42 LYY76 pKa = 10.94 LAGDD80 pKa = 4.1 WLTEE84 pKa = 3.97 TGLLDD89 pKa = 4.2 NPVQVAFGYY98 pKa = 10.23 GKK100 pKa = 10.25 IMLMTEE106 pKa = 3.82 SDD108 pKa = 5.07 LIPVV112 pKa = 4.08
Molecular weight: 12.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.872
IPC_protein 3.808
Toseland 3.605
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.643
Grimsley 3.528
Solomon 3.795
Lehninger 3.745
Nozaki 3.935
DTASelect 4.139
Thurlkill 3.668
EMBOSS 3.757
Sillero 3.935
Patrickios 0.846
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.849
Protein with the highest isoelectric point:
>tr|D3V7A4|D3V7A4_XENBS Response regulator in two-component regulatory system with NarQ (Or NarX) regulates anaerobic respiratory gene expression OS=Xenorhabdus bovienii (strain SS-2004) OX=406818 GN=narP PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.68 RR12 pKa = 11.84 NRR14 pKa = 11.84 THH16 pKa = 7.1 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.48 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.36 GRR39 pKa = 11.84 TRR41 pKa = 11.84 LTVSKK46 pKa = 10.95
Molecular weight: 5.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4102
0
4102
1169299
20
9647
285.1
31.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.225 ± 0.051
1.089 ± 0.015
5.21 ± 0.034
5.942 ± 0.033
3.958 ± 0.027
6.864 ± 0.044
2.399 ± 0.022
6.721 ± 0.041
5.093 ± 0.058
10.596 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.586 ± 0.027
4.346 ± 0.037
4.265 ± 0.042
4.703 ± 0.041
5.315 ± 0.032
6.354 ± 0.038
5.397 ± 0.031
6.407 ± 0.031
1.367 ± 0.016
3.163 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here