Acinetobacter phage AB1
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E2GM03|E2GM03_9CAUD AB1gp65 OS=Acinetobacter phage AB1 OX=889876 GN=AB1-65 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.23 NALILASSVALAACSGKK19 pKa = 9.62 PVITGPYY26 pKa = 8.41 EE27 pKa = 4.1 VEE29 pKa = 4.22 SLDD32 pKa = 3.67 MEE34 pKa = 5.01 HH35 pKa = 6.49 NVAAIKK41 pKa = 10.92 SGDD44 pKa = 3.47 LVLEE48 pKa = 4.32 VEE50 pKa = 4.35 FEE52 pKa = 4.21 GRR54 pKa = 11.84 FFKK57 pKa = 11.12 DD58 pKa = 2.6 GDD60 pKa = 4.21 GFQSWNDD67 pKa = 3.43 VEE69 pKa = 4.54 IKK71 pKa = 10.46 SVSDD75 pKa = 3.14 VKK77 pKa = 11.24 VYY79 pKa = 11.17 NEE81 pKa = 4.27 DD82 pKa = 4.01 GEE84 pKa = 4.64 TEE86 pKa = 4.86 DD87 pKa = 3.85 YY88 pKa = 11.0 VLPSEE93 pKa = 4.24 EE94 pKa = 3.83 VSNIVQVIEE103 pKa = 4.04 NEE105 pKa = 3.82 IAEE108 pKa = 4.25 NMM110 pKa = 3.84
Molecular weight: 12.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.234
IPC2_protein 4.024
IPC_protein 3.935
Toseland 3.77
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.05
Wikipedia 3.77
Rodwell 3.77
Grimsley 3.681
Solomon 3.884
Lehninger 3.834
Nozaki 4.012
DTASelect 4.139
Thurlkill 3.795
EMBOSS 3.783
Sillero 4.05
Patrickios 3.503
IPC_peptide 3.884
IPC2_peptide 4.037
IPC2.peptide.svr19 3.968
Protein with the highest isoelectric point:
>tr|E2GLZ4|E2GLZ4_9CAUD AB1gp56 OS=Acinetobacter phage AB1 OX=889876 GN=AB1-56 PE=4 SV=1
MM1 pKa = 7.62 SKK3 pKa = 10.18 PATSFLFLGEE13 pKa = 4.57 LKK15 pKa = 10.3 PLEE18 pKa = 4.18 STVIEE23 pKa = 3.99 NTKK26 pKa = 8.36 RR27 pKa = 11.84 TKK29 pKa = 10.5 VRR31 pKa = 11.84 DD32 pKa = 3.78 AIRR35 pKa = 11.84 RR36 pKa = 11.84 YY37 pKa = 10.37 GDD39 pKa = 3.17 RR40 pKa = 11.84 KK41 pKa = 10.6 FNTTQVGNNVIVTRR55 pKa = 11.84 IQQ57 pKa = 2.88
Molecular weight: 6.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.277
IPC2_protein 9.677
IPC_protein 10.262
Toseland 10.877
ProMoST 10.482
Dawson 10.935
Bjellqvist 10.584
Wikipedia 11.111
Rodwell 11.272
Grimsley 10.965
Solomon 11.067
Lehninger 11.038
Nozaki 10.833
DTASelect 10.599
Thurlkill 10.847
EMBOSS 11.272
Sillero 10.862
Patrickios 11.052
IPC_peptide 11.067
IPC2_peptide 9.151
IPC2.peptide.svr19 8.762
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
85
0
85
13582
37
763
159.8
17.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.995 ± 0.322
1.156 ± 0.119
6.398 ± 0.257
6.568 ± 0.395
4.477 ± 0.26
6.774 ± 0.282
1.576 ± 0.164
7.193 ± 0.305
7.606 ± 0.47
7.797 ± 0.269
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.776 ± 0.19
5.971 ± 0.318
3.151 ± 0.19
3.991 ± 0.238
3.659 ± 0.198
6.582 ± 0.26
5.228 ± 0.41
6.752 ± 0.25
1.406 ± 0.103
3.946 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here