Penicillium freii

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11722 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A101MFJ0|A0A101MFJ0_9EURO Uncharacterized protein OS=Penicillium freii OX=48697 GN=ACN42_g7456 PE=4 SV=1
MM1 pKa = 7.13TFNVFLTGLLAATAAALPHH20 pKa = 5.95SARR23 pKa = 11.84SGEE26 pKa = 4.15SGAVEE31 pKa = 4.28TRR33 pKa = 11.84DD34 pKa = 3.5SDD36 pKa = 3.82SGSLQIVNNMDD47 pKa = 3.43TNVYY51 pKa = 10.12LWTTSSDD58 pKa = 3.31AGSMKK63 pKa = 10.11TLSTGGDD70 pKa = 3.74YY71 pKa = 10.92SEE73 pKa = 4.65KK74 pKa = 10.17WVTNSNGGGISIKK87 pKa = 10.03MSTSEE92 pKa = 4.55SEE94 pKa = 4.54DD95 pKa = 3.2SVLQFEE101 pKa = 4.64YY102 pKa = 10.25TQDD105 pKa = 3.4GDD107 pKa = 3.76TLYY110 pKa = 10.59WDD112 pKa = 4.05MSSIDD117 pKa = 4.36LDD119 pKa = 3.86STSEE123 pKa = 3.99FVKK126 pKa = 10.7SGFSVVPSDD135 pKa = 4.2SSCKK139 pKa = 9.39TVSCAAGDD147 pKa = 4.24ADD149 pKa = 5.95CKK151 pKa = 10.73DD152 pKa = 4.2AYY154 pKa = 10.6QLPDD158 pKa = 3.43DD159 pKa = 4.56VNTYY163 pKa = 10.45SCSLSAGFTLTLGG176 pKa = 3.67

Molecular weight: 18.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A101MEX0|A0A101MEX0_9EURO Uncharacterized protein OS=Penicillium freii OX=48697 GN=ACN42_g7797 PE=4 SV=1
MM1 pKa = 7.49PVSPSRR7 pKa = 11.84TRR9 pKa = 11.84PSTAGRR15 pKa = 11.84TMSTTTSVPLPRR27 pKa = 11.84ARR29 pKa = 11.84TSVPASSSTTPSALFAPRR47 pKa = 11.84PGPIVGTVSARR58 pKa = 11.84LATSLSTLTSRR69 pKa = 5.13

Molecular weight: 7.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11722

0

11722

5271666

8

6432

449.7

49.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.373 ± 0.019

1.22 ± 0.009

5.644 ± 0.015

6.095 ± 0.024

3.835 ± 0.016

6.77 ± 0.02

2.437 ± 0.01

5.134 ± 0.018

4.624 ± 0.018

8.997 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.261 ± 0.007

3.753 ± 0.011

6.059 ± 0.022

4.05 ± 0.016

5.911 ± 0.021

8.372 ± 0.025

6.034 ± 0.019

6.167 ± 0.016

1.471 ± 0.008

2.792 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski