Amino acid dipepetide frequency for Penicillium freii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.347AlaAla: 8.347 ± 0.052	1.044AlaCys: 1.044 ± 0.015	4.19AlaAsp: 4.19 ± 0.03	4.987AlaGlu: 4.987 ± 0.041	3.182AlaPhe: 3.182 ± 0.03	5.646AlaGly: 5.646 ± 0.041	1.802AlaHis: 1.802 ± 0.019	4.405AlaIle: 4.405 ± 0.031	3.821AlaLys: 3.821 ± 0.032	7.656AlaLeu: 7.656 ± 0.049	2.025AlaMet: 2.025 ± 0.018	2.987AlaAsn: 2.987 ± 0.025	4.757AlaPro: 4.757 ± 0.045	3.354AlaGln: 3.354 ± 0.029	4.703AlaArg: 4.703 ± 0.032	7.037AlaSer: 7.037 ± 0.043	5.199AlaThr: 5.199 ± 0.032	5.306AlaVal: 5.306 ± 0.036	1.14AlaTrp: 1.14 ± 0.013	2.142AlaTyr: 2.142 ± 0.022	0.0AlaXaa: 0.0 ± 0.0
Cys	0.942CysAla: 0.942 ± 0.015	0.23CysCys: 0.23 ± 0.007	0.655CysAsp: 0.655 ± 0.011	0.592CysGlu: 0.592 ± 0.011	0.571CysPhe: 0.571 ± 0.011	0.91CysGly: 0.91 ± 0.014	0.34CysHis: 0.34 ± 0.008	0.729CysIle: 0.729 ± 0.012	0.468CysLys: 0.468 ± 0.01	1.291CysLeu: 1.291 ± 0.016	0.278CysMet: 0.278 ± 0.008	0.43CysAsn: 0.43 ± 0.009	0.639CysPro: 0.639 ± 0.013	0.462CysGln: 0.462 ± 0.01	0.722CysArg: 0.722 ± 0.011	0.892CysSer: 0.892 ± 0.014	0.68CysThr: 0.68 ± 0.012	0.815CysVal: 0.815 ± 0.013	0.203CysTrp: 0.203 ± 0.007	0.352CysTyr: 0.352 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	4.474AspAla: 4.474 ± 0.03	0.605AspCys: 0.605 ± 0.011	3.864AspAsp: 3.864 ± 0.04	4.255AspGlu: 4.255 ± 0.034	2.165AspPhe: 2.165 ± 0.022	3.856AspGly: 3.856 ± 0.032	1.346AspHis: 1.346 ± 0.016	3.222AspIle: 3.222 ± 0.028	2.248AspLys: 2.248 ± 0.022	5.208AspLeu: 5.208 ± 0.033	1.307AspMet: 1.307 ± 0.017	1.937AspAsn: 1.937 ± 0.022	3.394AspPro: 3.394 ± 0.028	2.043AspGln: 2.043 ± 0.019	3.084AspArg: 3.084 ± 0.027	4.265AspSer: 4.265 ± 0.029	3.07AspThr: 3.07 ± 0.027	3.609AspVal: 3.609 ± 0.028	0.896AspTrp: 0.896 ± 0.012	1.591AspTyr: 1.591 ± 0.017	0.0AspXaa: 0.0 ± 0.0
Glu	5.113GluAla: 5.113 ± 0.039	0.621GluCys: 0.621 ± 0.011	4.119GluAsp: 4.119 ± 0.035	5.206GluGlu: 5.206 ± 0.054	1.986GluPhe: 1.986 ± 0.021	3.583GluGly: 3.583 ± 0.029	1.42GluHis: 1.42 ± 0.016	3.219GluIle: 3.219 ± 0.029	3.503GluLys: 3.503 ± 0.031	5.111GluLeu: 5.111 ± 0.04	1.504GluMet: 1.504 ± 0.017	2.374GluAsn: 2.374 ± 0.02	2.861GluPro: 2.861 ± 0.038	2.459GluGln: 2.459 ± 0.024	3.722GluArg: 3.722 ± 0.035	4.46GluSer: 4.46 ± 0.031	3.653GluThr: 3.653 ± 0.033	3.482GluVal: 3.482 ± 0.027	0.862GluTrp: 0.862 ± 0.014	1.694GluTyr: 1.694 ± 0.019	0.0GluXaa: 0.0 ± 0.0
Phe	3.098PheAla: 3.098 ± 0.026	0.581PheCys: 0.581 ± 0.012	2.35PheAsp: 2.35 ± 0.022	2.15PheGlu: 2.15 ± 0.021	1.799PhePhe: 1.799 ± 0.026	2.924PheGly: 2.924 ± 0.027	0.945PheHis: 0.945 ± 0.013	1.957PheIle: 1.957 ± 0.024	1.495PheLys: 1.495 ± 0.02	3.589PheLeu: 3.589 ± 0.032	0.871PheMet: 0.871 ± 0.014	1.517PheAsn: 1.517 ± 0.017	2.045PhePro: 2.045 ± 0.022	1.472PheGln: 1.472 ± 0.017	1.97PheArg: 1.97 ± 0.019	3.079PheSer: 3.079 ± 0.028	2.197PheThr: 2.197 ± 0.023	2.464PheVal: 2.464 ± 0.025	0.68PheTrp: 0.68 ± 0.012	1.164PheTyr: 1.164 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	5.228GlyAla: 5.228 ± 0.039	0.886GlyCys: 0.886 ± 0.016	3.56GlyAsp: 3.56 ± 0.026	3.508GlyGlu: 3.508 ± 0.026	2.873GlyPhe: 2.873 ± 0.024	5.369GlyGly: 5.369 ± 0.057	1.688GlyHis: 1.688 ± 0.022	3.654GlyIle: 3.654 ± 0.031	3.269GlyLys: 3.269 ± 0.029	6.219GlyLeu: 6.219 ± 0.043	1.634GlyMet: 1.634 ± 0.022	2.562GlyAsn: 2.562 ± 0.024	3.378GlyPro: 3.378 ± 0.032	2.575GlyGln: 2.575 ± 0.024	3.907GlyArg: 3.907 ± 0.031	5.708GlySer: 5.708 ± 0.043	3.97GlyThr: 3.97 ± 0.033	4.402GlyVal: 4.402 ± 0.033	1.159GlyTrp: 1.159 ± 0.016	2.146GlyTyr: 2.146 ± 0.021	0.0GlyXaa: 0.0 ± 0.0
His	1.84HisAla: 1.84 ± 0.02	0.34HisCys: 0.34 ± 0.008	1.334HisAsp: 1.334 ± 0.014	1.359HisGlu: 1.359 ± 0.018	0.963HisPhe: 0.963 ± 0.016	1.734HisGly: 1.734 ± 0.02	0.83HisHis: 0.83 ± 0.016	1.283HisIle: 1.283 ± 0.015	0.879HisLys: 0.879 ± 0.014	2.283HisLeu: 2.283 ± 0.024	0.535HisMet: 0.535 ± 0.01	0.892HisAsn: 0.892 ± 0.012	1.724HisPro: 1.724 ± 0.018	0.995HisGln: 0.995 ± 0.013	1.573HisArg: 1.573 ± 0.017	1.927HisSer: 1.927 ± 0.023	1.336HisThr: 1.336 ± 0.016	1.468HisVal: 1.468 ± 0.018	0.365HisTrp: 0.365 ± 0.008	0.709HisTyr: 0.709 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	4.344IleAla: 4.344 ± 0.03	0.813IleCys: 0.813 ± 0.013	2.944IleAsp: 2.944 ± 0.026	2.968IleGlu: 2.968 ± 0.027	2.163IlePhe: 2.163 ± 0.026	3.417IleGly: 3.417 ± 0.03	1.28IleHis: 1.28 ± 0.017	2.689IleIle: 2.689 ± 0.028	2.166IleLys: 2.166 ± 0.021	4.758IleLeu: 4.758 ± 0.039	1.105IleMet: 1.105 ± 0.016	1.868IleAsn: 1.868 ± 0.02	3.321IlePro: 3.321 ± 0.025	2.05IleGln: 2.05 ± 0.022	2.81IleArg: 2.81 ± 0.025	4.136IleSer: 4.136 ± 0.028	2.921IleThr: 2.921 ± 0.026	3.299IleVal: 3.299 ± 0.026	0.76IleTrp: 0.76 ± 0.012	1.524IleTyr: 1.524 ± 0.02	0.0IleXaa: 0.0 ± 0.0
Lys	3.927LysAla: 3.927 ± 0.035	0.47LysCys: 0.47 ± 0.009	2.659LysAsp: 2.659 ± 0.021	3.179LysGlu: 3.179 ± 0.03	1.459LysPhe: 1.459 ± 0.017	2.818LysGly: 2.818 ± 0.027	1.075LysHis: 1.075 ± 0.015	2.24LysIle: 2.24 ± 0.021	3.099LysLys: 3.099 ± 0.037	3.873LysLeu: 3.873 ± 0.028	1.019LysMet: 1.019 ± 0.014	1.733LysAsn: 1.733 ± 0.02	2.661LysPro: 2.661 ± 0.025	1.81LysGln: 1.81 ± 0.019	3.159LysArg: 3.159 ± 0.031	3.549LysSer: 3.549 ± 0.03	2.758LysThr: 2.758 ± 0.025	2.722LysVal: 2.722 ± 0.024	0.678LysTrp: 0.678 ± 0.011	1.353LysTyr: 1.353 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	7.79LeuAla: 7.79 ± 0.041	1.239LeuCys: 1.239 ± 0.016	5.238LeuAsp: 5.238 ± 0.034	5.469LeuGlu: 5.469 ± 0.039	3.5LeuPhe: 3.5 ± 0.03	6.065LeuGly: 6.065 ± 0.032	2.256LeuHis: 2.256 ± 0.023	4.171LeuIle: 4.171 ± 0.036	4.039LeuLys: 4.039 ± 0.03	8.439LeuLeu: 8.439 ± 0.063	1.9LeuMet: 1.9 ± 0.02	3.311LeuAsn: 3.311 ± 0.028	5.461LeuPro: 5.461 ± 0.038	3.907LeuGln: 3.907 ± 0.032	5.621LeuArg: 5.621 ± 0.041	7.514LeuSer: 7.514 ± 0.046	4.867LeuThr: 4.867 ± 0.033	5.579LeuVal: 5.579 ± 0.039	1.206LeuTrp: 1.206 ± 0.017	2.399LeuTyr: 2.399 ± 0.022	0.0LeuXaa: 0.0 ± 0.0
Met	2.213MetAla: 2.213 ± 0.021	0.263MetCys: 0.263 ± 0.007	1.306MetAsp: 1.306 ± 0.016	1.331MetGlu: 1.331 ± 0.013	0.817MetPhe: 0.817 ± 0.014	1.576MetGly: 1.576 ± 0.02	0.532MetHis: 0.532 ± 0.011	1.115MetIle: 1.115 ± 0.016	1.01MetLys: 1.01 ± 0.014	1.923MetLeu: 1.923 ± 0.022	0.614MetMet: 0.614 ± 0.012	0.867MetAsn: 0.867 ± 0.015	1.263MetPro: 1.263 ± 0.018	0.932MetGln: 0.932 ± 0.014	1.272MetArg: 1.272 ± 0.016	1.977MetSer: 1.977 ± 0.019	1.38MetThr: 1.38 ± 0.017	1.405MetVal: 1.405 ± 0.016	0.279MetTrp: 0.279 ± 0.007	0.53MetTyr: 0.53 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	3.145AsnAla: 3.145 ± 0.026	0.45AsnCys: 0.45 ± 0.009	1.98AsnAsp: 1.98 ± 0.019	2.118AsnGlu: 2.118 ± 0.023	1.435AsnPhe: 1.435 ± 0.019	2.877AsnGly: 2.877 ± 0.03	0.889AsnHis: 0.889 ± 0.014	2.144AsnIle: 2.144 ± 0.02	1.544AsnLys: 1.544 ± 0.015	3.37AsnLeu: 3.37 ± 0.026	0.913AsnMet: 0.913 ± 0.014	1.484AsnAsn: 1.484 ± 0.018	2.632AsnPro: 2.632 ± 0.025	1.432AsnGln: 1.432 ± 0.018	1.984AsnArg: 1.984 ± 0.015	2.809AsnSer: 2.809 ± 0.026	2.285AsnThr: 2.285 ± 0.023	2.364AsnVal: 2.364 ± 0.021	0.584AsnTrp: 0.584 ± 0.012	1.091AsnTyr: 1.091 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	5.187ProAla: 5.187 ± 0.042	0.52ProCys: 0.52 ± 0.011	3.221ProAsp: 3.221 ± 0.027	3.918ProGlu: 3.918 ± 0.035	2.125ProPhe: 2.125 ± 0.019	3.873ProGly: 3.873 ± 0.03	1.38ProHis: 1.38 ± 0.024	2.722ProIle: 2.722 ± 0.022	2.614ProLys: 2.614 ± 0.028	4.776ProLeu: 4.776 ± 0.028	1.164ProMet: 1.164 ± 0.018	2.281ProAsn: 2.281 ± 0.025	4.633ProPro: 4.633 ± 0.057	2.489ProGln: 2.489 ± 0.028	3.41ProArg: 3.41 ± 0.034	6.101ProSer: 6.101 ± 0.056	4.185ProThr: 4.185 ± 0.035	3.706ProVal: 3.706 ± 0.03	0.773ProTrp: 0.773 ± 0.013	1.515ProTyr: 1.515 ± 0.018	0.0ProXaa: 0.0 ± 0.0
Gln	3.343GlnAla: 3.343 ± 0.029	0.435GlnCys: 0.435 ± 0.01	2.055GlnAsp: 2.055 ± 0.021	2.397GlnGlu: 2.397 ± 0.02	1.407GlnPhe: 1.407 ± 0.019	2.445GlnGly: 2.445 ± 0.025	1.072GlnHis: 1.072 ± 0.012	2.019GlnIle: 2.019 ± 0.021	1.979GlnLys: 1.979 ± 0.025	3.495GlnLeu: 3.495 ± 0.028	0.954GlnMet: 0.954 ± 0.015	1.652GlnAsn: 1.652 ± 0.023	2.609GlnPro: 2.609 ± 0.034	2.19GlnGln: 2.19 ± 0.038	2.568GlnArg: 2.568 ± 0.023	3.342GlnSer: 3.342 ± 0.033	2.477GlnThr: 2.477 ± 0.023	2.253GlnVal: 2.253 ± 0.022	0.599GlnTrp: 0.599 ± 0.011	1.211GlnTyr: 1.211 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	4.484ArgAla: 4.484 ± 0.031	0.673ArgCys: 0.673 ± 0.013	3.309ArgAsp: 3.309 ± 0.024	3.688ArgGlu: 3.688 ± 0.032	2.154ArgPhe: 2.154 ± 0.022	3.641ArgGly: 3.641 ± 0.035	1.518ArgHis: 1.518 ± 0.018	2.877ArgIle: 2.877 ± 0.025	3.241ArgLys: 3.241 ± 0.028	5.382ArgLeu: 5.382 ± 0.034	1.313ArgMet: 1.313 ± 0.017	2.206ArgAsn: 2.206 ± 0.022	3.392ArgPro: 3.392 ± 0.033	2.543ArgGln: 2.543 ± 0.024	4.706ArgArg: 4.706 ± 0.04	4.753ArgSer: 4.753 ± 0.047	3.266ArgThr: 3.266 ± 0.024	3.418ArgVal: 3.418 ± 0.023	0.918ArgTrp: 0.918 ± 0.012	1.633ArgTyr: 1.633 ± 0.016	0.0ArgXaa: 0.0 ± 0.0
Ser	6.67SerAla: 6.67 ± 0.041	0.86SerCys: 0.86 ± 0.014	4.387SerAsp: 4.387 ± 0.034	4.335SerGlu: 4.335 ± 0.031	3.155SerPhe: 3.155 ± 0.026	5.593SerGly: 5.593 ± 0.042	2.078SerHis: 2.078 ± 0.022	4.215SerIle: 4.215 ± 0.031	3.654SerLys: 3.654 ± 0.031	7.495SerLeu: 7.495 ± 0.037	1.814SerMet: 1.814 ± 0.019	3.108SerAsn: 3.108 ± 0.028	5.637SerPro: 5.637 ± 0.057	3.393SerGln: 3.393 ± 0.034	4.874SerArg: 4.874 ± 0.045	8.66SerSer: 8.66 ± 0.076	5.724SerThr: 5.724 ± 0.039	4.797SerVal: 4.797 ± 0.034	1.165SerTrp: 1.165 ± 0.016	2.106SerTyr: 2.106 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	5.132ThrAla: 5.132 ± 0.03	0.732ThrCys: 0.732 ± 0.013	2.978ThrAsp: 2.978 ± 0.025	3.344ThrGlu: 3.344 ± 0.028	2.271ThrPhe: 2.271 ± 0.021	4.277ThrGly: 4.277 ± 0.033	1.335ThrHis: 1.335 ± 0.017	3.199ThrIle: 3.199 ± 0.028	2.601ThrLys: 2.601 ± 0.025	5.344ThrLeu: 5.344 ± 0.036	1.253ThrMet: 1.253 ± 0.016	2.188ThrAsn: 2.188 ± 0.018	4.403ThrPro: 4.403 ± 0.037	2.244ThrGln: 2.244 ± 0.022	3.158ThrArg: 3.158 ± 0.026	5.378ThrSer: 5.378 ± 0.042	4.218ThrThr: 4.218 ± 0.045	3.797ThrVal: 3.797 ± 0.03	0.858ThrTrp: 0.858 ± 0.012	1.626ThrTyr: 1.626 ± 0.019	0.0ThrXaa: 0.0 ± 0.0
Val	5.172ValAla: 5.172 ± 0.035	0.846ValCys: 0.846 ± 0.013	3.776ValAsp: 3.776 ± 0.03	3.767ValGlu: 3.767 ± 0.034	2.575ValPhe: 2.575 ± 0.024	4.059ValGly: 4.059 ± 0.03	1.453ValHis: 1.453 ± 0.017	3.193ValIle: 3.193 ± 0.028	2.735ValLys: 2.735 ± 0.024	5.666ValLeu: 5.666 ± 0.043	1.359ValMet: 1.359 ± 0.017	2.318ValAsn: 2.318 ± 0.024	3.639ValPro: 3.639 ± 0.03	2.434ValGln: 2.434 ± 0.021	3.317ValArg: 3.317 ± 0.025	4.899ValSer: 4.899 ± 0.032	3.574ValThr: 3.574 ± 0.032	4.265ValVal: 4.265 ± 0.039	0.86ValTrp: 0.86 ± 0.012	1.765ValTyr: 1.765 ± 0.022	0.0ValXaa: 0.0 ± 0.0
Trp	1.142TrpAla: 1.142 ± 0.017	0.198TrpCys: 0.198 ± 0.006	0.902TrpAsp: 0.902 ± 0.017	0.846TrpGlu: 0.846 ± 0.012	0.531TrpPhe: 0.531 ± 0.01	0.948TrpGly: 0.948 ± 0.015	0.354TrpHis: 0.354 ± 0.009	0.801TrpIle: 0.801 ± 0.012	0.815TrpLys: 0.815 ± 0.012	1.36TrpLeu: 1.36 ± 0.017	0.387TrpMet: 0.387 ± 0.009	0.66TrpAsn: 0.66 ± 0.011	0.601TrpPro: 0.601 ± 0.011	0.561TrpGln: 0.561 ± 0.012	0.94TrpArg: 0.94 ± 0.013	1.11TrpSer: 1.11 ± 0.015	0.934TrpThr: 0.934 ± 0.015	0.899TrpVal: 0.899 ± 0.015	0.286TrpTrp: 0.286 ± 0.008	0.44TrpTyr: 0.44 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.14TyrAla: 2.14 ± 0.02	0.395TyrCys: 0.395 ± 0.008	1.612TyrAsp: 1.612 ± 0.018	1.534TyrGlu: 1.534 ± 0.018	1.215TyrPhe: 1.215 ± 0.017	2.085TyrGly: 2.085 ± 0.025	0.777TyrHis: 0.777 ± 0.011	1.482TyrIle: 1.482 ± 0.018	1.063TyrLys: 1.063 ± 0.016	2.733TyrLeu: 2.733 ± 0.027	0.656TyrMet: 0.656 ± 0.011	1.153TyrAsn: 1.153 ± 0.018	1.542TyrPro: 1.542 ± 0.022	1.158TyrGln: 1.158 ± 0.013	1.613TyrArg: 1.613 ± 0.019	2.124TyrSer: 2.124 ± 0.023	1.645TyrThr: 1.645 ± 0.017	1.624TyrVal: 1.624 ± 0.019	0.44TyrTrp: 0.44 ± 0.009	0.932TyrTyr: 0.932 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 11722 proteins (5271666 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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