Acinetobacter phage AP205
Average proteome isoelectric point is 8.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9AZ42|Q9AZ42_9VIRU Coat protein OS=Acinetobacter phage AP205 OX=154784 PE=1 SV=1
MM1 pKa = 7.72 LNACDD6 pKa = 3.9 QLKK9 pKa = 8.86 STSIPAFQSNVLSDD23 pKa = 3.84 VLSLSDD29 pKa = 3.98 AADD32 pKa = 3.01 ITVKK36 pKa = 10.44 HH37 pKa = 6.31 RR38 pKa = 11.84 IVSKK42 pKa = 10.32 FGEE45 pKa = 4.28 PAGSSLRR52 pKa = 11.84 DD53 pKa = 3.31 VAFNNYY59 pKa = 9.75 KK60 pKa = 10.54 LFEE63 pKa = 4.05 QHH65 pKa = 6.57 LGSIPQITNLWQEE78 pKa = 3.83 GKK80 pKa = 10.64 EE81 pKa = 3.97 FFFLRR86 pKa = 11.84 KK87 pKa = 9.76 AKK89 pKa = 10.77 ANLGKK94 pKa = 9.44 WLKK97 pKa = 9.16 TFKK100 pKa = 10.64 LDD102 pKa = 3.64 YY103 pKa = 11.07 NSITVEE109 pKa = 4.0 FTPGEE114 pKa = 4.57 SYY116 pKa = 10.86 TSATGHH122 pKa = 5.39 VSVFAKK128 pKa = 10.53 LSNLAHH134 pKa = 5.93 WTCTADD140 pKa = 4.03 VVDD143 pKa = 5.09 DD144 pKa = 4.1 VCHH147 pKa = 5.67 LVYY150 pKa = 10.93 YY151 pKa = 10.81 NRR153 pKa = 11.84 GLKK156 pKa = 9.71 AAARR160 pKa = 11.84 KK161 pKa = 9.57 HH162 pKa = 5.68 IGLMVPIEE170 pKa = 4.41 GEE172 pKa = 4.11 SGFDD176 pKa = 3.02 TFSRR180 pKa = 11.84 HH181 pKa = 4.55 LMGVISIVPGARR193 pKa = 11.84 GASVPKK199 pKa = 10.36 NQEE202 pKa = 3.04 TDD204 pKa = 2.9 RR205 pKa = 11.84 FIDD208 pKa = 3.58 VEE210 pKa = 4.29 PTFNMILQRR219 pKa = 11.84 WVAGEE224 pKa = 3.62 ITRR227 pKa = 11.84 CLTLAKK233 pKa = 10.04 NHH235 pKa = 6.44 LGASRR240 pKa = 11.84 NINGKK245 pKa = 8.33 VVFHH249 pKa = 7.44 DD250 pKa = 3.47 AQEE253 pKa = 4.03 LHH255 pKa = 7.02 KK256 pKa = 11.09 EE257 pKa = 4.17 MIRR260 pKa = 11.84 DD261 pKa = 3.93 LSYY264 pKa = 10.82 ATIDD268 pKa = 3.6 FSNASDD274 pKa = 4.67 SVLLWVVQLLFPKK287 pKa = 10.03 HH288 pKa = 5.2 VSYY291 pKa = 11.57 VLTQYY296 pKa = 10.82 RR297 pKa = 11.84 SSTVQLGSDD306 pKa = 4.1 LIEE309 pKa = 4.53 PNKK312 pKa = 10.03 LSSMGNGFTFEE323 pKa = 4.2 VMTLLLLSIGRR334 pKa = 11.84 IFDD337 pKa = 3.56 PTCRR341 pKa = 11.84 VYY343 pKa = 11.34 GDD345 pKa = 3.84 DD346 pKa = 4.19 VIIKK350 pKa = 10.65 AEE352 pKa = 4.04 VADD355 pKa = 4.4 DD356 pKa = 4.65 FINTVSSIAFMTNNKK371 pKa = 8.38 KK372 pKa = 8.16 TFLKK376 pKa = 10.7 GLFRR380 pKa = 11.84 EE381 pKa = 4.49 SCGAFQFDD389 pKa = 3.82 TFDD392 pKa = 3.23 IQSFEE397 pKa = 4.69 FEE399 pKa = 4.04 WADD402 pKa = 3.35 NFTDD406 pKa = 5.81 VIAICNKK413 pKa = 9.96 LKK415 pKa = 10.98 LIIDD419 pKa = 4.49 AAQCNEE425 pKa = 3.6 AVIAILRR432 pKa = 11.84 NAHH435 pKa = 5.55 TVICEE440 pKa = 4.43 CIPVLCKK447 pKa = 10.62 GPQPPDD453 pKa = 3.41 FNLFLSQYY461 pKa = 10.75 VYY463 pKa = 11.43 DD464 pKa = 5.08 DD465 pKa = 3.2 NWKK468 pKa = 9.4 KK469 pKa = 10.97 KK470 pKa = 7.46 QMKK473 pKa = 9.85 SDD475 pKa = 3.74 LAITKK480 pKa = 10.11 LNRR483 pKa = 11.84 LVDD486 pKa = 3.89 KK487 pKa = 10.97 QWGFFSATHH496 pKa = 5.57 HH497 pKa = 6.52 HH498 pKa = 7.09 PEE500 pKa = 3.84 EE501 pKa = 4.18 LCYY504 pKa = 11.18 VNIPVYY510 pKa = 10.26 VPRR513 pKa = 11.84 RR514 pKa = 11.84 DD515 pKa = 3.63 SVHH518 pKa = 6.7 AGQNLFVDD526 pKa = 4.8 LSNLYY531 pKa = 10.55 ALRR534 pKa = 11.84 FTKK537 pKa = 9.8 STVRR541 pKa = 11.84 GKK543 pKa = 10.73 GKK545 pKa = 9.22 WVNVPHH551 pKa = 7.02 WVTPVGSIYY560 pKa = 9.84 RR561 pKa = 11.84 ASRR564 pKa = 11.84 IRR566 pKa = 11.84 QQYY569 pKa = 8.68 PNIGEE574 pKa = 4.91 LPTCYY579 pKa = 9.69 WSPHH583 pKa = 4.8 QLDD586 pKa = 5.71 LITSS590 pKa = 4.18
Molecular weight: 66.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.675
IPC2_protein 6.825
IPC_protein 6.825
Toseland 6.781
ProMoST 7.293
Dawson 7.41
Bjellqvist 7.585
Wikipedia 7.351
Rodwell 7.424
Grimsley 6.883
Solomon 7.439
Lehninger 7.454
Nozaki 7.892
DTASelect 7.658
Thurlkill 7.702
EMBOSS 7.717
Sillero 7.907
Patrickios 4.228
IPC_peptide 7.439
IPC2_peptide 7.19
IPC2.peptide.svr19 7.015
Protein with the highest isoelectric point:
>tr|Q9AZ45|Q9AZ45_9VIRU Lysis protein OS=Acinetobacter phage AP205 OX=154784 PE=4 SV=1
MM1 pKa = 7.39 NMYY4 pKa = 9.94 KK5 pKa = 9.81 WVPEE9 pKa = 4.24 SIRR12 pKa = 11.84 DD13 pKa = 3.66 SGEE16 pKa = 3.87 GQPSYY21 pKa = 11.61 SNNGDD26 pKa = 3.77 YY27 pKa = 10.89 APSGPWVAAGIHH39 pKa = 5.7 TMPQSLRR46 pKa = 11.84 DD47 pKa = 3.63 SMRR50 pKa = 11.84 NSIMVTAQARR60 pKa = 11.84 RR61 pKa = 11.84 DD62 pKa = 3.76 VIGPEE67 pKa = 3.67 WGPDD71 pKa = 3.27 GRR73 pKa = 11.84 FTGYY77 pKa = 10.83 ASVIGTPDD85 pKa = 3.92 PKK87 pKa = 10.65 PADD90 pKa = 3.27 IVNKK94 pKa = 8.17 FTVEE98 pKa = 3.69 RR99 pKa = 11.84 RR100 pKa = 11.84 PVSNGNFQQRR110 pKa = 11.84 VKK112 pKa = 11.07 AGDD115 pKa = 3.48 IVVAPYY121 pKa = 10.31 TSDD124 pKa = 2.99 GKK126 pKa = 9.55 ITVKK130 pKa = 10.5 LVAGQKK136 pKa = 10.14 DD137 pKa = 3.55 ISSTPDD143 pKa = 2.86 YY144 pKa = 10.68 DD145 pKa = 3.68 YY146 pKa = 11.21 RR147 pKa = 11.84 IDD149 pKa = 3.74 SSLASSAGFVVAGEE163 pKa = 3.62 RR164 pKa = 11.84 WYY166 pKa = 8.65 YY167 pKa = 8.15 TKK169 pKa = 10.6 RR170 pKa = 11.84 HH171 pKa = 6.14 FIIPRR176 pKa = 11.84 YY177 pKa = 7.01 FQNWRR182 pKa = 11.84 MRR184 pKa = 11.84 RR185 pKa = 11.84 RR186 pKa = 11.84 KK187 pKa = 9.79 YY188 pKa = 8.05 VTGWVMPTFYY198 pKa = 10.71 SPKK201 pKa = 10.09 EE202 pKa = 3.51 IFNRR206 pKa = 11.84 LKK208 pKa = 10.93 DD209 pKa = 3.65 SLVPDD214 pKa = 3.99 TGLVTQVWADD224 pKa = 3.54 NNTKK228 pKa = 10.58 RR229 pKa = 11.84 MDD231 pKa = 3.69 FLTAMAEE238 pKa = 4.18 IPQTLSSFLDD248 pKa = 3.23 ALGYY252 pKa = 10.31 LGSLIKK258 pKa = 10.54 DD259 pKa = 4.05 FKK261 pKa = 10.69 RR262 pKa = 11.84 RR263 pKa = 11.84 RR264 pKa = 11.84 FFLNKK269 pKa = 8.26 AHH271 pKa = 6.24 QRR273 pKa = 11.84 IRR275 pKa = 11.84 NKK277 pKa = 10.54 LGVSFAEE284 pKa = 3.87 RR285 pKa = 11.84 RR286 pKa = 11.84 SQIVSKK292 pKa = 10.22 YY293 pKa = 9.33 DD294 pKa = 3.28 RR295 pKa = 11.84 KK296 pKa = 10.03 IASARR301 pKa = 11.84 KK302 pKa = 7.89 PAIIVKK308 pKa = 9.84 LRR310 pKa = 11.84 QRR312 pKa = 11.84 KK313 pKa = 8.38 EE314 pKa = 3.83 KK315 pKa = 10.57 ALKK318 pKa = 10.51 ALDD321 pKa = 3.35 KK322 pKa = 10.69 MRR324 pKa = 11.84 VRR326 pKa = 11.84 EE327 pKa = 4.19 EE328 pKa = 3.49 KK329 pKa = 10.92 KK330 pKa = 9.89 MIRR333 pKa = 11.84 EE334 pKa = 4.27 FATQAASLWLSFRR347 pKa = 11.84 YY348 pKa = 9.84 EE349 pKa = 4.0 IMPLYY354 pKa = 10.12 YY355 pKa = 10.08 QSQDD359 pKa = 3.51 VLDD362 pKa = 4.87 VIANSTSEE370 pKa = 4.05 FMTSRR375 pKa = 11.84 DD376 pKa = 3.65 FVAKK380 pKa = 10.13 AINIGIPLEE389 pKa = 4.14 WNLDD393 pKa = 3.57 QEE395 pKa = 4.78 NLVSQPRR402 pKa = 11.84 HH403 pKa = 4.02 NVMVKK408 pKa = 10.43 SKK410 pKa = 10.74 LSPEE414 pKa = 3.91 NNIGKK419 pKa = 7.59 TLSVNPFTTAWEE431 pKa = 4.11 LLTLSFVVDD440 pKa = 3.13 WFVNFGDD447 pKa = 4.23 VIAGFTGGYY456 pKa = 9.51 SDD458 pKa = 6.04 DD459 pKa = 4.26 SGATASWRR467 pKa = 11.84 FDD469 pKa = 3.61 DD470 pKa = 5.31 KK471 pKa = 11.53 KK472 pKa = 11.25 VFHH475 pKa = 6.96 LKK477 pKa = 10.34 NIPSAMVIVDD487 pKa = 2.93 INFYY491 pKa = 8.72 TRR493 pKa = 11.84 QVIDD497 pKa = 3.59 PRR499 pKa = 11.84 LCGGLAFSPKK509 pKa = 10.36 LNLFRR514 pKa = 11.84 YY515 pKa = 9.22 LDD517 pKa = 3.93 AMSLSWNRR525 pKa = 11.84 SRR527 pKa = 11.84 LKK529 pKa = 10.38 ISRR532 pKa = 11.84 ATT534 pKa = 3.32
Molecular weight: 60.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.141
IPC2_protein 9.326
IPC_protein 9.37
Toseland 10.014
ProMoST 9.706
Dawson 10.218
Bjellqvist 9.882
Wikipedia 10.394
Rodwell 10.599
Grimsley 10.292
Solomon 10.233
Lehninger 10.204
Nozaki 9.984
DTASelect 9.882
Thurlkill 10.058
EMBOSS 10.409
Sillero 10.131
Patrickios 10.028
IPC_peptide 10.233
IPC2_peptide 8.404
IPC2.peptide.svr19 8.407
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1290
35
590
322.5
36.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.364 ± 1.087
1.24 ± 0.64
5.736 ± 0.955
3.721 ± 0.475
5.504 ± 0.935
5.271 ± 0.626
1.938 ± 0.702
6.202 ± 0.248
6.202 ± 0.357
8.45 ± 1.534
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.326 ± 0.589
5.194 ± 0.655
4.574 ± 0.513
3.488 ± 0.18
5.814 ± 1.33
8.14 ± 0.718
5.969 ± 1.203
7.519 ± 0.567
2.016 ± 0.355
3.333 ± 0.958
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here