Pediculus humanus subsp. corporis (Body louse)
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10788 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E0VTR4|E0VTR4_PEDHC PDZ domain-containing protein OS=Pediculus humanus subsp. corporis OX=121224 GN=8230578 PE=4 SV=1
AA1 pKa = 7.56 NVSNGYY7 pKa = 7.88 VHH9 pKa = 6.75 NAHH12 pKa = 6.55 SSVSNYY18 pKa = 9.7 LQSLLSQLGGSYY30 pKa = 10.17 KK31 pKa = 10.45 KK32 pKa = 10.38 PSTTSPLTTSSTTEE46 pKa = 3.65 YY47 pKa = 10.77 KK48 pKa = 10.71 LEE50 pKa = 4.14 EE51 pKa = 4.38 EE52 pKa = 4.57 EE53 pKa = 6.18 EE54 pKa = 4.12 EE55 pKa = 4.9 DD56 pKa = 4.69 DD57 pKa = 4.64 WIPDD61 pKa = 3.54 SLEE64 pKa = 4.06 GQLEE68 pKa = 4.23 DD69 pKa = 4.2 EE70 pKa = 4.44 NAIAARR76 pKa = 11.84 TTTTTPATGSSYY88 pKa = 11.25 NPQQYY93 pKa = 9.41 PPVYY97 pKa = 10.02 EE98 pKa = 4.36 EE99 pKa = 4.55 EE100 pKa = 5.29 EE101 pKa = 4.08 EE102 pKa = 4.73 DD103 pKa = 4.34 DD104 pKa = 4.45 WIPDD108 pKa = 3.54 SLEE111 pKa = 4.06 GQLEE115 pKa = 4.23 DD116 pKa = 4.2 EE117 pKa = 4.44 NAIAARR123 pKa = 11.84 TTTTTTTTTTPSPALNII140 pKa = 4.03
Molecular weight: 15.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.872
IPC_protein 3.783
Toseland 3.617
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.617
Rodwell 3.63
Grimsley 3.541
Solomon 3.732
Lehninger 3.681
Nozaki 3.872
DTASelect 3.973
Thurlkill 3.643
EMBOSS 3.643
Sillero 3.897
Patrickios 0.896
IPC_peptide 3.732
IPC2_peptide 3.884
IPC2.peptide.svr19 3.815
Protein with the highest isoelectric point:
>tr|E0VCK8|E0VCK8_PEDHC Uncharacterized protein OS=Pediculus humanus subsp. corporis OX=121224 GN=8231676 PE=4 SV=1
MM1 pKa = 7.34 VFFLRR6 pKa = 11.84 ISVSAFGGSQIMSAHH21 pKa = 5.86 KK22 pKa = 9.34 TFKK25 pKa = 10.44 IKK27 pKa = 10.81 AKK29 pKa = 9.72 LAKK32 pKa = 9.76 KK33 pKa = 10.26 LKK35 pKa = 8.98 QNRR38 pKa = 11.84 PIPQWVRR45 pKa = 11.84 MRR47 pKa = 11.84 TGNTIRR53 pKa = 11.84 YY54 pKa = 5.79 NAKK57 pKa = 8.47 RR58 pKa = 11.84 RR59 pKa = 11.84 HH60 pKa = 4.1 WRR62 pKa = 11.84 RR63 pKa = 11.84 TKK65 pKa = 10.89 LKK67 pKa = 10.55 LL68 pKa = 3.39
Molecular weight: 8.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 11.067
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.574
Bjellqvist 12.559
Wikipedia 13.027
Rodwell 12.427
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.149
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.981
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10781
7
10788
5547409
45
10733
514.2
58.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.687 ± 0.024
1.968 ± 0.023
5.512 ± 0.026
7.086 ± 0.039
4.261 ± 0.02
5.367 ± 0.031
2.226 ± 0.016
6.2 ± 0.024
8.095 ± 0.048
9.058 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.125 ± 0.01
6.327 ± 0.028
4.718 ± 0.033
3.82 ± 0.02
4.501 ± 0.021
8.404 ± 0.034
5.54 ± 0.02
5.82 ± 0.019
1.037 ± 0.009
3.246 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here