Puniceibacterium sp. IMCC21224

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Puniceibacterium; unclassified Puniceibacterium

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5212 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J5QKQ7|A0A0J5QKQ7_9RHOB Dual specificity phosphatase OS=Puniceibacterium sp. IMCC21224 OX=1618204 GN=IMCC21224_113407 PE=4 SV=1
MM1 pKa = 6.74SQSVVVGGFRR11 pKa = 11.84RR12 pKa = 11.84FVYY15 pKa = 10.84GLMACTTFGATQAQAGDD32 pKa = 4.0FQIDD36 pKa = 3.2WSTFDD41 pKa = 3.27WPAGVTGPLVRR52 pKa = 11.84TLRR55 pKa = 11.84DD56 pKa = 3.25QYY58 pKa = 11.46GFEE61 pKa = 3.9VDD63 pKa = 3.12ATVEE67 pKa = 3.96HH68 pKa = 6.46TGIFSNYY75 pKa = 9.41SGSTPSPDD83 pKa = 3.13DD84 pKa = 3.4VAIFGGNVEE93 pKa = 4.19SLIIVSDD100 pKa = 3.97APQNLGAIGDD110 pKa = 3.92SRR112 pKa = 11.84TISTVSASSGGIAFPVDD129 pKa = 3.45SLTIDD134 pKa = 3.9VLDD137 pKa = 4.88IDD139 pKa = 5.7ANDD142 pKa = 3.8NNSTTDD148 pKa = 2.85RR149 pKa = 11.84CDD151 pKa = 4.13FITAFGNNGNPTMAALSATPSVIAGPGPGSGLTGALGANQAQCIYY196 pKa = 10.64VEE198 pKa = 4.69GFAGSPTSPNDD209 pKa = 3.25DD210 pKa = 3.58SGTIRR215 pKa = 11.84ATFPDD220 pKa = 3.75ATSSVTFWYY229 pKa = 10.15DD230 pKa = 3.06EE231 pKa = 4.41SIQNVRR237 pKa = 11.84NYY239 pKa = 11.32ASFINYY245 pKa = 9.63DD246 pKa = 3.26PGARR250 pKa = 11.84GIGMFGNANFSVDD263 pKa = 3.43QSISLSRR270 pKa = 11.84SATPSTGTQGEE281 pKa = 4.96TVTYY285 pKa = 9.05TYY287 pKa = 10.74TVTNTGSLPFNPGQDD302 pKa = 3.55VLIEE306 pKa = 5.03DD307 pKa = 4.79DD308 pKa = 4.26LLGTVTCNTISTLIAPGGTVTCEE331 pKa = 3.41APYY334 pKa = 9.99TITAADD340 pKa = 3.91VLTGSVDD347 pKa = 3.7SNAVAGIGAIGQPFVARR364 pKa = 11.84LQSDD368 pKa = 4.06TQPLSVVSSVILGGGGGAQNCTPQSVFAQPRR399 pKa = 11.84TQLAGPGTNTNMTTSDD415 pKa = 3.02IFLFDD420 pKa = 5.49DD421 pKa = 3.63VTVDD425 pKa = 3.96NNGNPIDD432 pKa = 4.23VVFQLDD438 pKa = 3.75QISNAINLEE447 pKa = 4.22LTSGLQARR455 pKa = 11.84MTPVDD460 pKa = 3.35NGYY463 pKa = 9.8VVYY466 pKa = 10.57RR467 pKa = 11.84LRR469 pKa = 11.84LVQDD473 pKa = 4.08GTATAANPLGTAIEE487 pKa = 3.91QSRR490 pKa = 11.84FNGIIVQQTDD500 pKa = 2.53IDD502 pKa = 4.04SRR504 pKa = 11.84GPGDD508 pKa = 4.54DD509 pKa = 3.65STDD512 pKa = 3.08VGGPIEE518 pKa = 4.38TPTTISHH525 pKa = 7.09FNTAPIASFPAGGNAIGQDD544 pKa = 3.5PAKK547 pKa = 10.62AGNPLDD553 pKa = 3.98WFDD556 pKa = 4.76EE557 pKa = 4.44PNEE560 pKa = 4.18TDD562 pKa = 3.52FDD564 pKa = 4.04NYY566 pKa = 9.73ATYY569 pKa = 10.2EE570 pKa = 3.81YY571 pKa = 9.47DD572 pKa = 3.4TFVEE576 pKa = 4.09ARR578 pKa = 11.84FIHH581 pKa = 7.09GYY583 pKa = 8.2TGTSTVPATRR593 pKa = 11.84GSGILLCAIANTSADD608 pKa = 4.08VIAEE612 pKa = 4.23DD613 pKa = 4.87DD614 pKa = 5.06DD615 pKa = 4.44YY616 pKa = 11.65TASPVNTLLGGTAGEE631 pKa = 4.41VMANDD636 pKa = 4.76TILGLPATPLNATLEE651 pKa = 4.47VLTEE655 pKa = 4.12AVPQNLGDD663 pKa = 4.02PVPLLEE669 pKa = 4.85TAGLDD674 pKa = 3.29AGRR677 pKa = 11.84VTVPAGVPAGVYY689 pKa = 8.51TIEE692 pKa = 4.01YY693 pKa = 8.7RR694 pKa = 11.84LCSAVDD700 pKa = 3.37PTDD703 pKa = 3.62CDD705 pKa = 3.31RR706 pKa = 11.84ARR708 pKa = 11.84VTIAVFEE715 pKa = 4.09GLGLDD720 pKa = 4.49FGDD723 pKa = 4.97APLSYY728 pKa = 9.48LTASHH733 pKa = 6.06TVGVVASVYY742 pKa = 10.72LGATPPDD749 pKa = 3.62IEE751 pKa = 5.78LVAQTDD757 pKa = 3.66ATATADD763 pKa = 4.17DD764 pKa = 4.86LLGTDD769 pKa = 5.26DD770 pKa = 4.41EE771 pKa = 5.98DD772 pKa = 4.66GLIFPVLTQGAVSTLEE788 pKa = 3.91VTVNGAGYY796 pKa = 9.23LQAWLDD802 pKa = 3.86FNGDD806 pKa = 3.77GLFEE810 pKa = 5.0DD811 pKa = 4.77ALGEE815 pKa = 4.75RR816 pKa = 11.84IGTDD820 pKa = 3.15LRR822 pKa = 11.84DD823 pKa = 5.07DD824 pKa = 3.7GTQFDD829 pKa = 5.04NIAGDD834 pKa = 3.95GVIQIDD840 pKa = 3.71VTVPANATTSLTFSRR855 pKa = 11.84FRR857 pKa = 11.84YY858 pKa = 8.64ATDD861 pKa = 2.98TGVVVAGLALDD872 pKa = 4.66GEE874 pKa = 4.91VEE876 pKa = 4.17DD877 pKa = 4.81HH878 pKa = 6.67SLIIAAADD886 pKa = 3.48LVDD889 pKa = 5.34RR890 pKa = 11.84GDD892 pKa = 3.89APASYY897 pKa = 10.23GDD899 pKa = 3.71PRR901 pKa = 11.84HH902 pKa = 5.89VVVPDD907 pKa = 3.86IYY909 pKa = 11.05LGAAEE914 pKa = 5.37PDD916 pKa = 4.11TEE918 pKa = 4.26TSPQNSVNADD928 pKa = 3.32GDD930 pKa = 4.12DD931 pKa = 4.38LADD934 pKa = 5.37LDD936 pKa = 5.77DD937 pKa = 4.86EE938 pKa = 5.66DD939 pKa = 5.11SVATFPVLEE948 pKa = 4.86AGTTVSLTVQTHH960 pKa = 4.76EE961 pKa = 4.39TLSAQLDD968 pKa = 4.08LPIPTLLPGITNLQLWIDD986 pKa = 3.88YY987 pKa = 8.34NQNGVFDD994 pKa = 4.2TSEE997 pKa = 4.58HH998 pKa = 5.91IAVDD1002 pKa = 3.73YY1003 pKa = 11.12RR1004 pKa = 11.84DD1005 pKa = 4.3GDD1007 pKa = 3.6TGDD1010 pKa = 3.34TDD1012 pKa = 4.71GVFNNQISFDD1022 pKa = 3.47ISVPADD1028 pKa = 2.96IGNGQSYY1035 pKa = 11.22ARR1037 pKa = 11.84LRR1039 pKa = 11.84WSTTSAVAADD1049 pKa = 4.52PFDD1052 pKa = 3.72GLNFDD1057 pKa = 5.41GEE1059 pKa = 4.57VEE1061 pKa = 4.53DD1062 pKa = 4.89YY1063 pKa = 11.15LVTLSNPNGPLSCTANIYY1081 pKa = 9.3MVATEE1086 pKa = 4.28TAQNLPALSEE1096 pKa = 4.04LAFTEE1101 pKa = 4.14SGGNYY1106 pKa = 8.54TLNQTLFPPDD1116 pKa = 3.21YY1117 pKa = 9.29TGNYY1121 pKa = 9.56LVTGWGYY1128 pKa = 11.46NEE1130 pKa = 4.84LDD1132 pKa = 3.61DD1133 pKa = 4.72YY1134 pKa = 11.4IYY1136 pKa = 10.99GVRR1139 pKa = 11.84QSPRR1143 pKa = 11.84TLMRR1147 pKa = 11.84INAAGAVQEE1156 pKa = 4.27VADD1159 pKa = 4.49LSGLGIEE1166 pKa = 5.15SPDD1169 pKa = 3.31STADD1173 pKa = 3.32ILPNGIMVYY1182 pKa = 9.98MSGTSNNIYY1191 pKa = 10.23QLLDD1195 pKa = 3.05ISDD1198 pKa = 4.07PANPVNLGVLTAGAGSPYY1216 pKa = 10.51GRR1218 pKa = 11.84DD1219 pKa = 2.68IAYY1222 pKa = 10.08NPRR1225 pKa = 11.84DD1226 pKa = 3.08GMMYY1230 pKa = 10.45FIDD1233 pKa = 4.26PNRR1236 pKa = 11.84DD1237 pKa = 3.02VYY1239 pKa = 11.14ILDD1242 pKa = 4.1PRR1244 pKa = 11.84DD1245 pKa = 3.72GVPGATTVTLVGNVPLPAGYY1265 pKa = 9.82GAIDD1269 pKa = 3.78IDD1271 pKa = 4.47SVWFDD1276 pKa = 3.28GSGFFYY1282 pKa = 10.69GFDD1285 pKa = 3.36NQSRR1289 pKa = 11.84QVFVVEE1295 pKa = 4.72LGGDD1299 pKa = 3.63GDD1301 pKa = 4.21RR1302 pKa = 11.84PGSFQFIEE1310 pKa = 4.18VLGTVQDD1317 pKa = 3.67LTYY1320 pKa = 10.52QGNDD1324 pKa = 3.46GASCRR1329 pKa = 11.84AFPGPFVSTVFLDD1342 pKa = 3.95GTISGTLYY1350 pKa = 10.82EE1351 pKa = 4.79DD1352 pKa = 4.19ANNSGVFDD1360 pKa = 4.31AGEE1363 pKa = 4.05TGLPVGITVEE1373 pKa = 4.71LYY1375 pKa = 10.85DD1376 pKa = 5.19EE1377 pKa = 4.95NGTPANFADD1386 pKa = 4.06DD1387 pKa = 3.93TLVTSTEE1394 pKa = 3.89TAADD1398 pKa = 3.66GTYY1401 pKa = 10.96SFISVDD1407 pKa = 2.83SSLTYY1412 pKa = 10.44RR1413 pKa = 11.84IQVDD1417 pKa = 3.57EE1418 pKa = 5.25ADD1420 pKa = 3.94PEE1422 pKa = 4.45IPASLVMSTANPITGVTVATGTEE1445 pKa = 4.21TPDD1448 pKa = 3.13QNFGFVTAAASADD1461 pKa = 3.53LSLTKK1466 pKa = 10.54VAIDD1470 pKa = 3.65VASGQPTTEE1479 pKa = 3.66ATAGTEE1485 pKa = 3.46IDD1487 pKa = 3.85FVLSVTNDD1495 pKa = 3.14GPGSVTGVRR1504 pKa = 11.84VRR1506 pKa = 11.84DD1507 pKa = 3.92QIPNGYY1513 pKa = 8.0TYY1515 pKa = 11.27VSDD1518 pKa = 4.12DD1519 pKa = 3.29AAAAGDD1525 pKa = 4.2TYY1527 pKa = 11.52ASSTGVWTVGDD1538 pKa = 3.85VANGATEE1545 pKa = 3.97VLTIRR1550 pKa = 11.84VTMNPTGEE1558 pKa = 4.15HH1559 pKa = 5.16TNRR1562 pKa = 11.84AEE1564 pKa = 4.1IVASSLPDD1572 pKa = 4.22PDD1574 pKa = 4.09SDD1576 pKa = 4.09PAVGALTDD1584 pKa = 4.1DD1585 pKa = 4.57LSDD1588 pKa = 3.79GVADD1592 pKa = 5.1DD1593 pKa = 5.44DD1594 pKa = 4.31EE1595 pKa = 6.65ASAAISFVGTGAILSGNVFEE1615 pKa = 6.19DD1616 pKa = 3.41NGAGGATAYY1625 pKa = 10.36DD1626 pKa = 3.91GLKK1629 pKa = 10.74GGTEE1633 pKa = 3.84PATNKK1638 pKa = 10.36AVVSVLNASSVLIGTPDD1655 pKa = 3.17VAADD1659 pKa = 4.25GSWSLTLPEE1668 pKa = 5.32GYY1670 pKa = 10.05SDD1672 pKa = 3.93AVTVSIAPAVGLRR1685 pKa = 11.84TVSEE1689 pKa = 4.4APGALPGLVNAAPRR1703 pKa = 11.84DD1704 pKa = 3.64GSFTFTPVPGVAYY1717 pKa = 10.61SDD1719 pKa = 4.36LNFGLIEE1726 pKa = 4.1VARR1729 pKa = 11.84LSQSQQSAIRR1739 pKa = 11.84AGQVVTLRR1747 pKa = 11.84HH1748 pKa = 6.25EE1749 pKa = 4.24YY1750 pKa = 10.61LADD1753 pKa = 3.53SDD1755 pKa = 4.01GTVIFDD1761 pKa = 4.33VDD1763 pKa = 3.48IQQASAPGLFSVGLFLDD1780 pKa = 4.63AACDD1784 pKa = 4.0GSVGAAVDD1792 pKa = 3.7GALPISANTRR1802 pKa = 11.84ICLVARR1808 pKa = 11.84VSVSSAASAGSTIGFDD1824 pKa = 3.66LVADD1828 pKa = 4.18TTYY1831 pKa = 10.97GASGLTEE1838 pKa = 3.72QDD1840 pKa = 3.02RR1841 pKa = 11.84NTDD1844 pKa = 3.62LVRR1847 pKa = 11.84VEE1849 pKa = 4.63SPNGALKK1856 pKa = 10.65LSKK1859 pKa = 9.14TVRR1862 pKa = 11.84NVTQGTPEE1870 pKa = 4.19GVSNGAAAGDD1880 pKa = 3.61VLEE1883 pKa = 4.01YY1884 pKa = 10.06RR1885 pKa = 11.84VYY1887 pKa = 11.1LEE1889 pKa = 4.33NPGVLPATDD1898 pKa = 3.55IVIHH1902 pKa = 6.96DD1903 pKa = 4.15RR1904 pKa = 11.84TPPYY1908 pKa = 9.89TVLATAIPSPVNVGGDD1924 pKa = 3.84VVCSVAAPGVNAGGYY1939 pKa = 9.49SGALRR1944 pKa = 11.84WEE1946 pKa = 4.47CTGTHH1951 pKa = 6.1RR1952 pKa = 11.84PGAIGAVSFQVQIAPP1967 pKa = 3.69

Molecular weight: 203.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J5QHI7|A0A0J5QHI7_9RHOB Lactate dehydrogenase-like oxidoreductase OS=Puniceibacterium sp. IMCC21224 OX=1618204 GN=IMCC21224_113144 PE=3 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.12RR4 pKa = 11.84TFQPSNLVRR13 pKa = 11.84KK14 pKa = 8.83HH15 pKa = 4.64RR16 pKa = 11.84HH17 pKa = 3.91GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.37AGRR29 pKa = 11.84KK30 pKa = 8.54ILNARR35 pKa = 11.84RR36 pKa = 11.84ASGRR40 pKa = 11.84KK41 pKa = 9.06SLSAA45 pKa = 4.0

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5212

0

5212

1636611

11

4622

314.0

34.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.14 ± 0.04

0.919 ± 0.014

6.26 ± 0.036

5.297 ± 0.03

3.695 ± 0.021

8.774 ± 0.041

2.014 ± 0.018

5.212 ± 0.026

2.861 ± 0.025

10.145 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.733 ± 0.018

2.64 ± 0.018

5.079 ± 0.027

3.331 ± 0.018

6.78 ± 0.038

5.416 ± 0.024

5.8 ± 0.027

7.346 ± 0.025

1.351 ± 0.014

2.208 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski