Amino acid dipepetide frequency for Puniceibacterium sp. IMCC21224

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.106AlaAla: 16.106 ± 0.131	1.129AlaCys: 1.129 ± 0.029	7.161AlaAsp: 7.161 ± 0.081	7.191AlaGlu: 7.191 ± 0.075	4.213AlaPhe: 4.213 ± 0.051	10.211AlaGly: 10.211 ± 0.094	2.222AlaHis: 2.222 ± 0.041	5.866AlaIle: 5.866 ± 0.066	3.307AlaLys: 3.307 ± 0.055	13.808AlaLeu: 13.808 ± 0.143	3.606AlaMet: 3.606 ± 0.051	2.809AlaAsn: 2.809 ± 0.046	5.662AlaPro: 5.662 ± 0.068	4.801AlaGln: 4.801 ± 0.064	8.592AlaArg: 8.592 ± 0.103	5.892AlaSer: 5.892 ± 0.064	6.49AlaThr: 6.49 ± 0.071	8.486AlaVal: 8.486 ± 0.084	1.379AlaTrp: 1.379 ± 0.03	2.465AlaTyr: 2.465 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	1.144CysAla: 1.144 ± 0.028	0.137CysCys: 0.137 ± 0.009	0.666CysAsp: 0.666 ± 0.022	0.428CysGlu: 0.428 ± 0.018	0.343CysPhe: 0.343 ± 0.014	0.935CysGly: 0.935 ± 0.027	0.258CysHis: 0.258 ± 0.016	0.45CysIle: 0.45 ± 0.017	0.197CysLys: 0.197 ± 0.011	0.898CysLeu: 0.898 ± 0.025	0.178CysMet: 0.178 ± 0.009	0.238CysAsn: 0.238 ± 0.013	0.532CysPro: 0.532 ± 0.022	0.264CysGln: 0.264 ± 0.015	0.586CysArg: 0.586 ± 0.02	0.466CysSer: 0.466 ± 0.016	0.468CysThr: 0.468 ± 0.019	0.654CysVal: 0.654 ± 0.019	0.126CysTrp: 0.126 ± 0.009	0.216CysTyr: 0.216 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.63AspAla: 7.63 ± 0.083	0.524AspCys: 0.524 ± 0.019	3.786AspAsp: 3.786 ± 0.074	3.214AspGlu: 3.214 ± 0.049	2.32AspPhe: 2.32 ± 0.036	5.934AspGly: 5.934 ± 0.074	1.387AspHis: 1.387 ± 0.035	3.182AspIle: 3.182 ± 0.047	1.609AspLys: 1.609 ± 0.033	6.715AspLeu: 6.715 ± 0.062	1.689AspMet: 1.689 ± 0.031	1.356AspAsn: 1.356 ± 0.029	3.616AspPro: 3.616 ± 0.053	1.933AspGln: 1.933 ± 0.033	4.393AspArg: 4.393 ± 0.057	2.556AspSer: 2.556 ± 0.043	3.527AspThr: 3.527 ± 0.06	4.531AspVal: 4.531 ± 0.065	1.194AspTrp: 1.194 ± 0.031	1.502AspTyr: 1.502 ± 0.03	0.0AspXaa: 0.0 ± 0.0
Glu	6.994GluAla: 6.994 ± 0.08	0.364GluCys: 0.364 ± 0.018	3.034GluAsp: 3.034 ± 0.041	2.756GluGlu: 2.756 ± 0.05	1.716GluPhe: 1.716 ± 0.035	4.049GluGly: 4.049 ± 0.05	1.042GluHis: 1.042 ± 0.027	3.417GluIle: 3.417 ± 0.047	1.779GluLys: 1.779 ± 0.041	4.731GluLeu: 4.731 ± 0.063	1.689GluMet: 1.689 ± 0.037	1.581GluAsn: 1.581 ± 0.029	2.333GluPro: 2.333 ± 0.039	1.972GluGln: 1.972 ± 0.036	3.928GluArg: 3.928 ± 0.054	2.249GluSer: 2.249 ± 0.037	3.695GluThr: 3.695 ± 0.051	3.978GluVal: 3.978 ± 0.057	0.628GluTrp: 0.628 ± 0.021	1.029GluTyr: 1.029 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	4.503PheAla: 4.503 ± 0.062	0.42PheCys: 0.42 ± 0.016	2.933PheAsp: 2.933 ± 0.049	2.147PheGlu: 2.147 ± 0.04	1.386PhePhe: 1.386 ± 0.032	3.832PheGly: 3.832 ± 0.053	0.743PheHis: 0.743 ± 0.023	1.534PheIle: 1.534 ± 0.03	0.832PheLys: 0.832 ± 0.026	3.477PheLeu: 3.477 ± 0.055	0.822PheMet: 0.822 ± 0.022	1.036PheAsn: 1.036 ± 0.025	1.539PhePro: 1.539 ± 0.03	1.077PheGln: 1.077 ± 0.024	2.26PheArg: 2.26 ± 0.039	2.164PheSer: 2.164 ± 0.04	1.974PheThr: 1.974 ± 0.038	2.825PheVal: 2.825 ± 0.046	0.532PheTrp: 0.532 ± 0.014	0.911PheTyr: 0.911 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	9.891GlyAla: 9.891 ± 0.093	0.869GlyCys: 0.869 ± 0.027	5.013GlyAsp: 5.013 ± 0.073	4.13GlyGlu: 4.13 ± 0.058	3.722GlyPhe: 3.722 ± 0.054	7.858GlyGly: 7.858 ± 0.117	1.922GlyHis: 1.922 ± 0.037	4.608GlyIle: 4.608 ± 0.057	2.848GlyLys: 2.848 ± 0.043	9.324GlyLeu: 9.324 ± 0.084	2.583GlyMet: 2.583 ± 0.043	2.321GlyAsn: 2.321 ± 0.058	3.7GlyPro: 3.7 ± 0.05	3.309GlyGln: 3.309 ± 0.052	5.717GlyArg: 5.717 ± 0.065	4.577GlySer: 4.577 ± 0.066	5.152GlyThr: 5.152 ± 0.072	6.499GlyVal: 6.499 ± 0.067	1.41GlyTrp: 1.41 ± 0.031	2.286GlyTyr: 2.286 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	2.212HisAla: 2.212 ± 0.04	0.243HisCys: 0.243 ± 0.012	1.286HisAsp: 1.286 ± 0.032	0.94HisGlu: 0.94 ± 0.024	0.826HisPhe: 0.826 ± 0.024	1.911HisGly: 1.911 ± 0.04	0.538HisHis: 0.538 ± 0.02	0.998HisIle: 0.998 ± 0.023	0.54HisLys: 0.54 ± 0.015	2.063HisLeu: 2.063 ± 0.037	0.541HisMet: 0.541 ± 0.018	0.461HisAsn: 0.461 ± 0.015	1.335HisPro: 1.335 ± 0.031	0.636HisGln: 0.636 ± 0.02	1.328HisArg: 1.328 ± 0.032	0.958HisSer: 0.958 ± 0.027	0.901HisThr: 0.901 ± 0.021	1.576HisVal: 1.576 ± 0.037	0.333HisTrp: 0.333 ± 0.014	0.519HisTyr: 0.519 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	6.959IleAla: 6.959 ± 0.067	0.619IleCys: 0.619 ± 0.019	3.561IleAsp: 3.561 ± 0.054	3.345IleGlu: 3.345 ± 0.051	1.789IlePhe: 1.789 ± 0.035	4.918IleGly: 4.918 ± 0.059	0.91IleHis: 0.91 ± 0.023	2.276IleIle: 2.276 ± 0.042	1.422IleLys: 1.422 ± 0.032	4.881IleLeu: 4.881 ± 0.074	1.044IleMet: 1.044 ± 0.029	1.398IleAsn: 1.398 ± 0.03	2.437IlePro: 2.437 ± 0.041	1.297IleGln: 1.297 ± 0.027	3.267IleArg: 3.267 ± 0.052	3.064IleSer: 3.064 ± 0.046	3.083IleThr: 3.083 ± 0.049	3.911IleVal: 3.911 ± 0.054	0.726IleTrp: 0.726 ± 0.022	1.212IleTyr: 1.212 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	3.438LysAla: 3.438 ± 0.057	0.175LysCys: 0.175 ± 0.011	1.547LysAsp: 1.547 ± 0.033	1.306LysGlu: 1.306 ± 0.034	0.838LysPhe: 0.838 ± 0.026	2.506LysGly: 2.506 ± 0.041	0.559LysHis: 0.559 ± 0.02	1.565LysIle: 1.565 ± 0.031	1.053LysLys: 1.053 ± 0.031	2.743LysLeu: 2.743 ± 0.052	0.821LysMet: 0.821 ± 0.022	0.716LysAsn: 0.716 ± 0.023	1.643LysPro: 1.643 ± 0.035	0.843LysGln: 0.843 ± 0.023	2.068LysArg: 2.068 ± 0.035	1.75LysSer: 1.75 ± 0.037	1.89LysThr: 1.89 ± 0.037	2.153LysVal: 2.153 ± 0.042	0.38LysTrp: 0.38 ± 0.015	0.61LysTyr: 0.61 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	12.51LeuAla: 12.51 ± 0.104	1.082LeuCys: 1.082 ± 0.026	6.331LeuAsp: 6.331 ± 0.062	4.935LeuGlu: 4.935 ± 0.065	3.578LeuPhe: 3.578 ± 0.062	8.731LeuGly: 8.731 ± 0.084	1.886LeuHis: 1.886 ± 0.036	5.37LeuIle: 5.37 ± 0.081	2.866LeuLys: 2.866 ± 0.046	9.188LeuLeu: 9.188 ± 0.115	2.717LeuMet: 2.717 ± 0.045	2.902LeuAsn: 2.902 ± 0.046	5.58LeuPro: 5.58 ± 0.074	2.772LeuGln: 2.772 ± 0.042	7.492LeuArg: 7.492 ± 0.086	7.014LeuSer: 7.014 ± 0.085	6.365LeuThr: 6.365 ± 0.06	6.905LeuVal: 6.905 ± 0.084	1.286LeuTrp: 1.286 ± 0.032	1.945LeuTyr: 1.945 ± 0.032	0.0LeuXaa: 0.0 ± 0.0
Met	3.392MetAla: 3.392 ± 0.048	0.192MetCys: 0.192 ± 0.01	1.393MetAsp: 1.393 ± 0.033	1.162MetGlu: 1.162 ± 0.027	0.845MetPhe: 0.845 ± 0.027	2.227MetGly: 2.227 ± 0.038	0.458MetHis: 0.458 ± 0.015	1.611MetIle: 1.611 ± 0.033	0.967MetLys: 0.967 ± 0.022	2.664MetLeu: 2.664 ± 0.042	0.758MetMet: 0.758 ± 0.024	0.827MetAsn: 0.827 ± 0.023	1.474MetPro: 1.474 ± 0.029	1.024MetGln: 1.024 ± 0.025	1.931MetArg: 1.931 ± 0.035	1.755MetSer: 1.755 ± 0.033	2.215MetThr: 2.215 ± 0.036	1.864MetVal: 1.864 ± 0.032	0.23MetTrp: 0.23 ± 0.012	0.342MetTyr: 0.342 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.234AsnAla: 3.234 ± 0.046	0.282AsnCys: 0.282 ± 0.013	1.696AsnAsp: 1.696 ± 0.051	1.126AsnGlu: 1.126 ± 0.029	0.972AsnPhe: 0.972 ± 0.029	2.473AsnGly: 2.473 ± 0.048	0.527AsnHis: 0.527 ± 0.019	1.375AsnIle: 1.375 ± 0.029	0.634AsnLys: 0.634 ± 0.024	2.581AsnLeu: 2.581 ± 0.038	0.712AsnMet: 0.712 ± 0.021	0.695AsnAsn: 0.695 ± 0.02	1.836AsnPro: 1.836 ± 0.038	0.746AsnGln: 0.746 ± 0.023	1.799AsnArg: 1.799 ± 0.029	1.251AsnSer: 1.251 ± 0.032	1.445AsnThr: 1.445 ± 0.033	1.902AsnVal: 1.902 ± 0.036	0.461AsnTrp: 0.461 ± 0.017	0.651AsnTyr: 0.651 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	5.426ProAla: 5.426 ± 0.061	0.375ProCys: 0.375 ± 0.017	4.127ProAsp: 4.127 ± 0.059	3.719ProGlu: 3.719 ± 0.049	1.923ProPhe: 1.923 ± 0.032	4.532ProGly: 4.532 ± 0.055	1.061ProHis: 1.061 ± 0.028	2.264ProIle: 2.264 ± 0.036	1.538ProLys: 1.538 ± 0.032	4.755ProLeu: 4.755 ± 0.051	1.352ProMet: 1.352 ± 0.032	1.352ProAsn: 1.352 ± 0.03	2.366ProPro: 2.366 ± 0.045	1.864ProGln: 1.864 ± 0.035	2.885ProArg: 2.885 ± 0.049	2.566ProSer: 2.566 ± 0.041	2.577ProThr: 2.577 ± 0.042	4.298ProVal: 4.298 ± 0.053	0.665ProTrp: 0.665 ± 0.018	1.148ProTyr: 1.148 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	4.163GlnAla: 4.163 ± 0.051	0.206GlnCys: 0.206 ± 0.011	1.829GlnAsp: 1.829 ± 0.037	1.485GlnGlu: 1.485 ± 0.032	1.227GlnPhe: 1.227 ± 0.027	2.725GlnGly: 2.725 ± 0.045	0.681GlnHis: 0.681 ± 0.02	2.215GlnIle: 2.215 ± 0.037	1.058GlnLys: 1.058 ± 0.029	2.9GlnLeu: 2.9 ± 0.046	1.091GlnMet: 1.091 ± 0.026	1.0GlnAsn: 1.0 ± 0.027	1.721GlnPro: 1.721 ± 0.034	1.163GlnGln: 1.163 ± 0.03	2.275GlnArg: 2.275 ± 0.042	2.008GlnSer: 2.008 ± 0.036	2.087GlnThr: 2.087 ± 0.042	2.488GlnVal: 2.488 ± 0.045	0.384GlnTrp: 0.384 ± 0.016	0.61GlnTyr: 0.61 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	8.091ArgAla: 8.091 ± 0.093	0.538ArgCys: 0.538 ± 0.016	4.545ArgAsp: 4.545 ± 0.06	3.426ArgGlu: 3.426 ± 0.044	2.67ArgPhe: 2.67 ± 0.043	4.76ArgGly: 4.76 ± 0.062	1.593ArgHis: 1.593 ± 0.033	3.969ArgIle: 3.969 ± 0.053	2.178ArgLys: 2.178 ± 0.041	7.333ArgLeu: 7.333 ± 0.086	1.928ArgMet: 1.928 ± 0.037	1.913ArgAsn: 1.913 ± 0.036	3.31ArgPro: 3.31 ± 0.052	2.355ArgGln: 2.355 ± 0.042	5.006ArgArg: 5.006 ± 0.068	3.494ArgSer: 3.494 ± 0.058	3.306ArgThr: 3.306 ± 0.057	4.801ArgVal: 4.801 ± 0.058	0.909ArgTrp: 0.909 ± 0.024	1.677ArgTyr: 1.677 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	6.206SerAla: 6.206 ± 0.064	0.461SerCys: 0.461 ± 0.017	3.692SerAsp: 3.692 ± 0.049	2.921SerGlu: 2.921 ± 0.049	2.247SerPhe: 2.247 ± 0.04	5.747SerGly: 5.747 ± 0.067	1.057SerHis: 1.057 ± 0.026	2.626SerIle: 2.626 ± 0.048	1.516SerLys: 1.516 ± 0.037	5.417SerLeu: 5.417 ± 0.073	1.455SerMet: 1.455 ± 0.029	1.399SerAsn: 1.399 ± 0.029	2.572SerPro: 2.572 ± 0.034	1.75SerGln: 1.75 ± 0.034	3.427SerArg: 3.427 ± 0.045	2.825SerSer: 2.825 ± 0.049	2.687SerThr: 2.687 ± 0.047	4.098SerVal: 4.098 ± 0.056	0.689SerTrp: 0.689 ± 0.021	1.371SerTyr: 1.371 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	6.564ThrAla: 6.564 ± 0.074	0.517ThrCys: 0.517 ± 0.019	3.519ThrAsp: 3.519 ± 0.052	2.988ThrGlu: 2.988 ± 0.044	2.048ThrPhe: 2.048 ± 0.038	5.775ThrGly: 5.775 ± 0.066	1.144ThrHis: 1.144 ± 0.026	2.951ThrIle: 2.951 ± 0.045	1.416ThrLys: 1.416 ± 0.028	6.514ThrLeu: 6.514 ± 0.073	1.389ThrMet: 1.389 ± 0.032	1.393ThrAsn: 1.393 ± 0.032	3.594ThrPro: 3.594 ± 0.051	1.879ThrGln: 1.879 ± 0.035	3.744ThrArg: 3.744 ± 0.055	3.036ThrSer: 3.036 ± 0.041	3.157ThrThr: 3.157 ± 0.05	4.35ThrVal: 4.35 ± 0.061	0.705ThrTrp: 0.705 ± 0.024	1.315ThrTyr: 1.315 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	9.14ValAla: 9.14 ± 0.091	0.676ValCys: 0.676 ± 0.019	4.087ValAsp: 4.087 ± 0.056	4.047ValGlu: 4.047 ± 0.048	2.861ValPhe: 2.861 ± 0.046	5.489ValGly: 5.489 ± 0.068	1.308ValHis: 1.308 ± 0.032	4.191ValIle: 4.191 ± 0.056	1.861ValLys: 1.861 ± 0.039	7.629ValLeu: 7.629 ± 0.093	2.071ValMet: 2.071 ± 0.036	1.972ValAsn: 1.972 ± 0.039	3.741ValPro: 3.741 ± 0.052	2.322ValGln: 2.322 ± 0.043	4.448ValArg: 4.448 ± 0.052	4.518ValSer: 4.518 ± 0.053	4.99ValThr: 4.99 ± 0.07	5.684ValVal: 5.684 ± 0.071	0.962ValTrp: 0.962 ± 0.027	1.462ValTyr: 1.462 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	1.275TrpAla: 1.275 ± 0.029	0.142TrpCys: 0.142 ± 0.01	0.803TrpAsp: 0.803 ± 0.026	0.548TrpGlu: 0.548 ± 0.019	0.531TrpPhe: 0.531 ± 0.017	0.992TrpGly: 0.992 ± 0.026	0.343TrpHis: 0.343 ± 0.015	0.719TrpIle: 0.719 ± 0.022	0.445TrpLys: 0.445 ± 0.017	1.539TrpLeu: 1.539 ± 0.032	0.422TrpMet: 0.422 ± 0.016	0.423TrpAsn: 0.423 ± 0.017	0.7TrpPro: 0.7 ± 0.024	0.617TrpGln: 0.617 ± 0.021	1.062TrpArg: 1.062 ± 0.026	0.825TrpSer: 0.825 ± 0.023	0.811TrpThr: 0.811 ± 0.026	0.865TrpVal: 0.865 ± 0.024	0.177TrpTrp: 0.177 ± 0.012	0.272TrpTyr: 0.272 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.518TyrAla: 2.518 ± 0.034	0.236TyrCys: 0.236 ± 0.012	1.587TyrAsp: 1.587 ± 0.033	1.151TyrGlu: 1.151 ± 0.028	0.89TyrPhe: 0.89 ± 0.025	2.134TyrGly: 2.134 ± 0.037	0.505TyrHis: 0.505 ± 0.019	0.929TyrIle: 0.929 ± 0.025	0.541TyrLys: 0.541 ± 0.019	2.294TyrLeu: 2.294 ± 0.039	0.461TyrMet: 0.461 ± 0.018	0.61TyrAsn: 0.61 ± 0.019	1.104TyrPro: 1.104 ± 0.027	0.689TyrGln: 0.689 ± 0.021	1.593TyrArg: 1.593 ± 0.034	1.196TyrSer: 1.196 ± 0.029	1.179TyrThr: 1.179 ± 0.027	1.592TyrVal: 1.592 ± 0.029	0.335TyrTrp: 0.335 ± 0.016	0.535TyrTyr: 0.535 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5212 proteins (1636611 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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