Intoshia linei

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Spiralia; Lophotrochozoa; Mesozoa; Orthonectida; Rhopaluridae; Intoshia

Average proteome isoelectric point is 7.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8720 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177AUG4|A0A177AUG4_9BILA Uncharacterized protein OS=Intoshia linei OX=1819745 GN=A3Q56_06684 PE=4 SV=1
MM1 pKa = 7.79VEE3 pKa = 4.16LLVNEE8 pKa = 4.62LSDD11 pKa = 4.57NEE13 pKa = 4.45LDD15 pKa = 3.6NASTVRR21 pKa = 11.84YY22 pKa = 9.36QEE24 pKa = 3.89NSVCIYY30 pKa = 10.8EE31 pKa = 4.22NDD33 pKa = 3.55SLLGIGTLLIYY44 pKa = 10.79DD45 pKa = 4.22NFCRR49 pKa = 11.84WRR51 pKa = 11.84SSKK54 pKa = 9.54TDD56 pKa = 3.17QIMDD60 pKa = 3.68INYY63 pKa = 10.1EE64 pKa = 4.39DD65 pKa = 3.85IGVHH69 pKa = 6.68AICRR73 pKa = 11.84DD74 pKa = 3.29KK75 pKa = 11.31TRR77 pKa = 11.84FPQNCIYY84 pKa = 11.12LMVDD88 pKa = 3.29KK89 pKa = 10.63FVEE92 pKa = 4.36YY93 pKa = 10.32EE94 pKa = 3.78NSEE97 pKa = 4.17NVPTDD102 pKa = 3.01IYY104 pKa = 11.42NEE106 pKa = 3.83IRR108 pKa = 11.84FVPQNEE114 pKa = 3.82ASIDD118 pKa = 3.87TIFNTMIQFVVSSEE132 pKa = 3.84SDD134 pKa = 3.22IEE136 pKa = 4.51NKK138 pKa = 10.23FSDD141 pKa = 4.4YY142 pKa = 11.11EE143 pKa = 4.46DD144 pKa = 3.89NGCQFDD150 pKa = 3.99EE151 pKa = 5.18ADD153 pKa = 4.12EE154 pKa = 4.29EE155 pKa = 4.83TNSDD159 pKa = 3.03GDD161 pKa = 3.59

Molecular weight: 18.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177AZ59|A0A177AZ59_9BILA ADF-H domain-containing protein OS=Intoshia linei OX=1819745 GN=A3Q56_05122 PE=3 SV=1
MM1 pKa = 5.43VTKK4 pKa = 10.51RR5 pKa = 11.84KK6 pKa = 9.72VGRR9 pKa = 11.84KK10 pKa = 5.25ATKK13 pKa = 8.74PKK15 pKa = 9.99VVKK18 pKa = 10.28KK19 pKa = 9.21VAVRR23 pKa = 11.84KK24 pKa = 6.56TVKK27 pKa = 10.34RR28 pKa = 11.84SAKK31 pKa = 9.57PKK33 pKa = 9.14RR34 pKa = 11.84KK35 pKa = 9.05VVKK38 pKa = 10.58KK39 pKa = 10.05IVKK42 pKa = 9.27KK43 pKa = 9.88VNRR46 pKa = 11.84ASAKK50 pKa = 9.57GKK52 pKa = 9.92KK53 pKa = 9.14SKK55 pKa = 10.51KK56 pKa = 9.92

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8720

0

8720

3312867

9

6337

379.9

43.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.439 ± 0.018

2.32 ± 0.029

5.561 ± 0.017

6.023 ± 0.027

4.924 ± 0.021

3.562 ± 0.033

2.183 ± 0.012

9.147 ± 0.026

9.308 ± 0.032

8.853 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.457 ± 0.011

8.442 ± 0.033

3.031 ± 0.019

3.445 ± 0.016

3.633 ± 0.02

8.007 ± 0.021

5.27 ± 0.023

5.104 ± 0.017

0.831 ± 0.007

4.459 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski