Brenneria salicis ATCC 15712 = DSM 30166

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Pectobacteriaceae; Brenneria; Brenneria salicis

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3611 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A366I6W9|A0A366I6W9_9GAMM GntR family transcriptional regulator OS=Brenneria salicis ATCC 15712 = DSM 30166 OX=714314 GN=DES54_11059 PE=4 SV=1
MM1 pKa = 7.74IIGFVLLVSACGTDD15 pKa = 3.66FCDD18 pKa = 5.8AIPVSDD24 pKa = 5.34DD25 pKa = 2.88IYY27 pKa = 11.32LNRR30 pKa = 11.84EE31 pKa = 3.8SCQLVMDD38 pKa = 4.51IVHH41 pKa = 6.61EE42 pKa = 4.16RR43 pKa = 11.84RR44 pKa = 11.84PDD46 pKa = 3.18AVLICGEE53 pKa = 4.08VQRR56 pKa = 11.84DD57 pKa = 3.64EE58 pKa = 5.28NEE60 pKa = 4.56GEE62 pKa = 4.2DD63 pKa = 3.77EE64 pKa = 4.17

Molecular weight: 7.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A366I7U7|A0A366I7U7_9GAMM Uncharacterized protein OS=Brenneria salicis ATCC 15712 = DSM 30166 OX=714314 GN=DES54_10567 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.36GRR39 pKa = 11.84TRR41 pKa = 11.84LTVSKK46 pKa = 10.95

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3611

0

3611

1120221

27

2954

310.2

34.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.74 ± 0.047

1.072 ± 0.014

5.314 ± 0.033

5.544 ± 0.037

3.721 ± 0.028

7.277 ± 0.038

2.279 ± 0.021

6.025 ± 0.037

3.991 ± 0.034

11.03 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.679 ± 0.021

3.73 ± 0.027

4.449 ± 0.027

4.807 ± 0.041

5.984 ± 0.033

6.031 ± 0.032

5.263 ± 0.03

6.88 ± 0.033

1.347 ± 0.019

2.835 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski