Ochrobactrum phage vB_OspP_OH

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XY23|A0A6G6XY23_9CAUD Uncharacterized protein OS=Ochrobactrum phage vB_OspP_OH OX=2712957 GN=phiOH_p63 PE=4 SV=1
MM1 pKa = 7.67NYY3 pKa = 10.23GPIYY7 pKa = 9.31MPIYY11 pKa = 10.56GGGEE15 pKa = 3.92GTAPAVTSAPSITGTAQVGQTLTATPAVFTGDD47 pKa = 3.5PAPTVTRR54 pKa = 11.84QWEE57 pKa = 4.27KK58 pKa = 10.15STGGAYY64 pKa = 10.05AAIEE68 pKa = 4.26GATDD72 pKa = 3.71LTYY75 pKa = 11.14DD76 pKa = 3.94PVVGDD81 pKa = 3.22VGATIRR87 pKa = 11.84VTEE90 pKa = 4.02MATNSKK96 pKa = 8.75GTVTSSSAPTAAVIAAEE113 pKa = 4.01

Molecular weight: 11.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XXL8|A0A6G6XXL8_9CAUD Uncharacterized protein OS=Ochrobactrum phage vB_OspP_OH OX=2712957 GN=phiOH_p18 PE=4 SV=1
MM1 pKa = 7.5SAPPGAATRR10 pKa = 11.84PRR12 pKa = 11.84CSTEE16 pKa = 4.27SPWTSYY22 pKa = 10.68RR23 pKa = 11.84SPRR26 pKa = 11.84VAAPPGGGSKK36 pKa = 10.47

Molecular weight: 3.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

13070

28

848

201.1

22.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.008 ± 0.57

0.796 ± 0.116

6.756 ± 0.247

6.572 ± 0.374

3.328 ± 0.241

7.896 ± 0.354

1.721 ± 0.176

5.164 ± 0.178

5.57 ± 0.25

6.664 ± 0.229

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.175 ± 0.171

4.376 ± 0.176

4.453 ± 0.248

3.917 ± 0.213

6.236 ± 0.253

5.738 ± 0.195

6.014 ± 0.249

6.756 ± 0.303

1.729 ± 0.166

3.129 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski