Amino acid dipepetide frequency for Ochrobactrum phage vB_OspP_OH

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.773AlaAla: 13.773 ± 2.328
0.995AlaCys: 0.995 ± 0.384
5.28AlaAsp: 5.28 ± 0.638
7.728AlaGlu: 7.728 ± 0.898
3.52AlaPhe: 3.52 ± 0.422
8.034AlaGly: 8.034 ± 1.109
1.454AlaHis: 1.454 ± 0.376
4.514AlaIle: 4.514 ± 0.518
6.657AlaLys: 6.657 ± 0.72
6.58AlaLeu: 6.58 ± 0.775
2.602AlaMet: 2.602 ± 0.511
3.214AlaAsn: 3.214 ± 0.517
4.744AlaPro: 4.744 ± 0.901
5.203AlaGln: 5.203 ± 0.782
6.427AlaArg: 6.427 ± 1.126
5.433AlaSer: 5.433 ± 0.67
4.974AlaThr: 4.974 ± 0.747
5.203AlaVal: 5.203 ± 0.761
1.224AlaTrp: 1.224 ± 0.325
2.525AlaTyr: 2.525 ± 0.559
0.0AlaXaa: 0.0 ± 0.0
Cys
0.459CysAla: 0.459 ± 0.264
0.077CysCys: 0.077 ± 0.094
0.689CysAsp: 0.689 ± 0.256
0.383CysGlu: 0.383 ± 0.225
0.306CysPhe: 0.306 ± 0.153
0.765CysGly: 0.765 ± 0.24
0.153CysHis: 0.153 ± 0.114
0.23CysIle: 0.23 ± 0.127
0.23CysLys: 0.23 ± 0.145
0.306CysLeu: 0.306 ± 0.152
0.23CysMet: 0.23 ± 0.137
0.383CysAsn: 0.383 ± 0.174
0.612CysPro: 0.612 ± 0.217
0.153CysGln: 0.153 ± 0.093
0.842CysArg: 0.842 ± 0.233
0.765CysSer: 0.765 ± 0.25
0.306CysThr: 0.306 ± 0.145
0.536CysVal: 0.536 ± 0.18
0.0CysTrp: 0.0 ± 0.0
0.536CysTyr: 0.536 ± 0.192
0.0CysXaa: 0.0 ± 0.0
Asp
8.264AspAla: 8.264 ± 0.83
0.306AspCys: 0.306 ± 0.139
4.285AspAsp: 4.285 ± 0.579
4.514AspGlu: 4.514 ± 0.775
2.372AspPhe: 2.372 ± 0.385
5.127AspGly: 5.127 ± 0.661
0.995AspHis: 0.995 ± 0.325
3.596AspIle: 3.596 ± 0.44
3.367AspLys: 3.367 ± 0.459
6.045AspLeu: 6.045 ± 0.634
2.831AspMet: 2.831 ± 0.53
3.137AspAsn: 3.137 ± 0.482
3.214AspPro: 3.214 ± 0.539
2.219AspGln: 2.219 ± 0.402
3.214AspArg: 3.214 ± 0.643
2.831AspSer: 2.831 ± 0.425
4.438AspThr: 4.438 ± 0.833
3.826AspVal: 3.826 ± 0.458
1.224AspTrp: 1.224 ± 0.264
1.76AspTyr: 1.76 ± 0.447
0.0AspXaa: 0.0 ± 0.0
Glu
7.04GluAla: 7.04 ± 1.026
0.765GluCys: 0.765 ± 0.207
4.591GluAsp: 4.591 ± 0.581
4.821GluGlu: 4.821 ± 0.912
1.989GluPhe: 1.989 ± 0.372
5.968GluGly: 5.968 ± 0.899
1.53GluHis: 1.53 ± 0.344
3.443GluIle: 3.443 ± 0.505
3.902GluLys: 3.902 ± 0.459
4.744GluLeu: 4.744 ± 0.625
3.29GluMet: 3.29 ± 0.546
2.755GluAsn: 2.755 ± 0.528
1.836GluPro: 1.836 ± 0.406
2.755GluGln: 2.755 ± 0.48
4.285GluArg: 4.285 ± 0.68
2.296GluSer: 2.296 ± 0.457
3.214GluThr: 3.214 ± 0.536
3.979GluVal: 3.979 ± 0.658
0.842GluTrp: 0.842 ± 0.241
1.683GluTyr: 1.683 ± 0.35
0.0GluXaa: 0.0 ± 0.0
Phe
2.908PheAla: 2.908 ± 0.346
0.383PheCys: 0.383 ± 0.183
2.372PheAsp: 2.372 ± 0.568
2.755PheGlu: 2.755 ± 0.552
0.612PhePhe: 0.612 ± 0.221
3.443PheGly: 3.443 ± 0.458
0.842PheHis: 0.842 ± 0.218
1.607PheIle: 1.607 ± 0.388
1.301PheLys: 1.301 ± 0.411
1.76PheLeu: 1.76 ± 0.364
0.995PheMet: 0.995 ± 0.257
2.066PheAsn: 2.066 ± 0.368
0.918PhePro: 0.918 ± 0.285
1.071PheGln: 1.071 ± 0.367
2.219PheArg: 2.219 ± 0.43
1.989PheSer: 1.989 ± 0.423
1.913PheThr: 1.913 ± 0.444
2.449PheVal: 2.449 ± 0.503
0.536PheTrp: 0.536 ± 0.217
1.148PheTyr: 1.148 ± 0.24
0.0PheXaa: 0.0 ± 0.0
Gly
7.652GlyAla: 7.652 ± 1.284
0.306GlyCys: 0.306 ± 0.141
5.739GlyAsp: 5.739 ± 0.81
4.285GlyGlu: 4.285 ± 0.547
2.296GlyPhe: 2.296 ± 0.385
8.264GlyGly: 8.264 ± 1.241
1.53GlyHis: 1.53 ± 0.334
4.361GlyIle: 4.361 ± 0.7
4.514GlyLys: 4.514 ± 0.649
4.208GlyLeu: 4.208 ± 0.695
2.066GlyMet: 2.066 ± 0.382
4.285GlyAsn: 4.285 ± 0.636
1.989GlyPro: 1.989 ± 0.405
2.449GlyGln: 2.449 ± 0.557
4.132GlyArg: 4.132 ± 0.629
5.05GlySer: 5.05 ± 0.789
5.05GlyThr: 5.05 ± 0.695
5.203GlyVal: 5.203 ± 0.603
2.296GlyTrp: 2.296 ± 0.496
3.29GlyTyr: 3.29 ± 0.467
0.0GlyXaa: 0.0 ± 0.0
His
1.071HisAla: 1.071 ± 0.347
0.306HisCys: 0.306 ± 0.167
1.301HisAsp: 1.301 ± 0.365
0.765HisGlu: 0.765 ± 0.283
0.842HisPhe: 0.842 ± 0.308
1.454HisGly: 1.454 ± 0.407
0.689HisHis: 0.689 ± 0.244
0.612HisIle: 0.612 ± 0.216
0.918HisLys: 0.918 ± 0.296
0.842HisLeu: 0.842 ± 0.275
0.383HisMet: 0.383 ± 0.185
0.459HisAsn: 0.459 ± 0.176
0.995HisPro: 0.995 ± 0.276
0.459HisGln: 0.459 ± 0.179
1.683HisArg: 1.683 ± 0.328
0.536HisSer: 0.536 ± 0.239
1.76HisThr: 1.76 ± 0.386
0.995HisVal: 0.995 ± 0.306
0.612HisTrp: 0.612 ± 0.205
0.536HisTyr: 0.536 ± 0.183
0.0HisXaa: 0.0 ± 0.0
Ile
4.668IleAla: 4.668 ± 0.758
0.383IleCys: 0.383 ± 0.169
4.514IleAsp: 4.514 ± 0.499
4.055IleGlu: 4.055 ± 0.605
1.607IlePhe: 1.607 ± 0.345
3.673IleGly: 3.673 ± 0.618
0.765IleHis: 0.765 ± 0.271
2.525IleIle: 2.525 ± 0.458
3.137IleLys: 3.137 ± 0.448
3.596IleLeu: 3.596 ± 0.461
1.454IleMet: 1.454 ± 0.352
2.142IleAsn: 2.142 ± 0.388
2.449IlePro: 2.449 ± 0.437
2.219IleGln: 2.219 ± 0.368
3.137IleArg: 3.137 ± 0.451
2.755IleSer: 2.755 ± 0.414
2.755IleThr: 2.755 ± 0.659
3.749IleVal: 3.749 ± 0.629
0.918IleTrp: 0.918 ± 0.308
1.148IleTyr: 1.148 ± 0.329
0.0IleXaa: 0.0 ± 0.0
Lys
5.356LysAla: 5.356 ± 0.734
0.153LysCys: 0.153 ± 0.103
3.979LysAsp: 3.979 ± 0.641
3.673LysGlu: 3.673 ± 0.533
1.989LysPhe: 1.989 ± 0.477
4.055LysGly: 4.055 ± 0.488
0.842LysHis: 0.842 ± 0.271
2.755LysIle: 2.755 ± 0.459
3.826LysLys: 3.826 ± 0.672
4.208LysLeu: 4.208 ± 0.633
1.989LysMet: 1.989 ± 0.384
2.066LysAsn: 2.066 ± 0.434
2.831LysPro: 2.831 ± 0.59
2.142LysGln: 2.142 ± 0.38
3.443LysArg: 3.443 ± 0.587
3.367LysSer: 3.367 ± 0.428
2.984LysThr: 2.984 ± 0.527
3.673LysVal: 3.673 ± 0.538
0.765LysTrp: 0.765 ± 0.262
1.607LysTyr: 1.607 ± 0.507
0.0LysXaa: 0.0 ± 0.0
Leu
8.034LeuAla: 8.034 ± 0.831
0.612LeuCys: 0.612 ± 0.215
4.897LeuAsp: 4.897 ± 0.684
3.979LeuGlu: 3.979 ± 0.703
1.454LeuPhe: 1.454 ± 0.376
4.668LeuGly: 4.668 ± 0.568
1.148LeuHis: 1.148 ± 0.401
2.525LeuIle: 2.525 ± 0.34
2.831LeuLys: 2.831 ± 0.504
4.514LeuLeu: 4.514 ± 0.568
2.296LeuMet: 2.296 ± 0.417
3.367LeuAsn: 3.367 ± 0.543
3.29LeuPro: 3.29 ± 0.642
2.449LeuGln: 2.449 ± 0.354
4.821LeuArg: 4.821 ± 0.624
4.821LeuSer: 4.821 ± 0.586
4.055LeuThr: 4.055 ± 0.677
3.826LeuVal: 3.826 ± 0.59
0.918LeuTrp: 0.918 ± 0.245
2.142LeuTyr: 2.142 ± 0.365
0.0LeuXaa: 0.0 ± 0.0
Met
3.902MetAla: 3.902 ± 0.494
0.306MetCys: 0.306 ± 0.171
1.301MetAsp: 1.301 ± 0.369
1.913MetGlu: 1.913 ± 0.465
1.148MetPhe: 1.148 ± 0.336
1.989MetGly: 1.989 ± 0.473
0.23MetHis: 0.23 ± 0.135
1.683MetIle: 1.683 ± 0.335
1.454MetLys: 1.454 ± 0.325
1.913MetLeu: 1.913 ± 0.498
0.995MetMet: 0.995 ± 0.31
1.76MetAsn: 1.76 ± 0.336
1.683MetPro: 1.683 ± 0.361
1.683MetGln: 1.683 ± 0.353
2.449MetArg: 2.449 ± 0.595
2.372MetSer: 2.372 ± 0.43
1.913MetThr: 1.913 ± 0.393
1.683MetVal: 1.683 ± 0.308
0.612MetTrp: 0.612 ± 0.245
0.765MetTyr: 0.765 ± 0.291
0.0MetXaa: 0.0 ± 0.0
Asn
3.596AsnAla: 3.596 ± 0.76
0.23AsnCys: 0.23 ± 0.147
2.755AsnAsp: 2.755 ± 0.515
3.137AsnGlu: 3.137 ± 0.542
1.301AsnPhe: 1.301 ± 0.283
3.826AsnGly: 3.826 ± 0.596
0.612AsnHis: 0.612 ± 0.184
2.372AsnIle: 2.372 ± 0.403
1.913AsnLys: 1.913 ± 0.337
2.525AsnLeu: 2.525 ± 0.459
1.071AsnMet: 1.071 ± 0.308
2.449AsnAsn: 2.449 ± 0.492
3.061AsnPro: 3.061 ± 0.514
1.454AsnGln: 1.454 ± 0.297
2.142AsnArg: 2.142 ± 0.37
2.296AsnSer: 2.296 ± 0.403
2.602AsnThr: 2.602 ± 0.499
3.749AsnVal: 3.749 ± 0.55
0.918AsnTrp: 0.918 ± 0.383
1.76AsnTyr: 1.76 ± 0.366
0.0AsnXaa: 0.0 ± 0.0
Pro
4.591ProAla: 4.591 ± 0.998
0.459ProCys: 0.459 ± 0.216
3.214ProAsp: 3.214 ± 0.467
3.29ProGlu: 3.29 ± 0.622
1.53ProPhe: 1.53 ± 0.305
2.066ProGly: 2.066 ± 0.408
0.765ProHis: 0.765 ± 0.274
2.296ProIle: 2.296 ± 0.465
3.673ProLys: 3.673 ± 0.694
1.683ProLeu: 1.683 ± 0.403
0.612ProMet: 0.612 ± 0.202
2.066ProAsn: 2.066 ± 0.419
2.296ProPro: 2.296 ± 0.469
1.224ProGln: 1.224 ± 0.327
3.214ProArg: 3.214 ± 0.381
2.219ProSer: 2.219 ± 0.436
3.367ProThr: 3.367 ± 0.531
3.749ProVal: 3.749 ± 0.457
0.536ProTrp: 0.536 ± 0.244
1.683ProTyr: 1.683 ± 0.483
0.0ProXaa: 0.0 ± 0.0
Gln
3.596GlnAla: 3.596 ± 0.71
0.23GlnCys: 0.23 ± 0.158
1.913GlnAsp: 1.913 ± 0.423
2.372GlnGlu: 2.372 ± 0.504
1.454GlnPhe: 1.454 ± 0.356
2.372GlnGly: 2.372 ± 0.439
0.689GlnHis: 0.689 ± 0.248
2.755GlnIle: 2.755 ± 0.446
1.989GlnLys: 1.989 ± 0.444
2.755GlnLeu: 2.755 ± 0.538
1.454GlnMet: 1.454 ± 0.361
1.53GlnAsn: 1.53 ± 0.379
1.836GlnPro: 1.836 ± 0.422
2.449GlnGln: 2.449 ± 0.605
2.984GlnArg: 2.984 ± 0.537
2.525GlnSer: 2.525 ± 0.484
2.296GlnThr: 2.296 ± 0.43
2.449GlnVal: 2.449 ± 0.381
0.689GlnTrp: 0.689 ± 0.221
0.842GlnTyr: 0.842 ± 0.272
0.0GlnXaa: 0.0 ± 0.0
Arg
4.668ArgAla: 4.668 ± 0.497
0.689ArgCys: 0.689 ± 0.248
4.132ArgAsp: 4.132 ± 0.642
4.438ArgGlu: 4.438 ± 0.795
1.913ArgPhe: 1.913 ± 0.454
3.443ArgGly: 3.443 ± 0.525
1.224ArgHis: 1.224 ± 0.356
3.902ArgIle: 3.902 ± 0.459
3.443ArgLys: 3.443 ± 0.736
4.361ArgLeu: 4.361 ± 0.636
2.219ArgMet: 2.219 ± 0.402
2.449ArgAsn: 2.449 ± 0.433
2.219ArgPro: 2.219 ± 0.394
2.219ArgGln: 2.219 ± 0.472
4.132ArgArg: 4.132 ± 0.643
3.902ArgSer: 3.902 ± 0.648
4.285ArgThr: 4.285 ± 0.57
4.974ArgVal: 4.974 ± 0.768
1.224ArgTrp: 1.224 ± 0.369
2.525ArgTyr: 2.525 ± 0.52
0.0ArgXaa: 0.0 ± 0.0
Ser
4.361SerAla: 4.361 ± 0.589
0.459SerCys: 0.459 ± 0.209
3.749SerAsp: 3.749 ± 0.473
3.214SerGlu: 3.214 ± 0.515
2.142SerPhe: 2.142 ± 0.441
5.05SerGly: 5.05 ± 0.628
1.071SerHis: 1.071 ± 0.334
3.367SerIle: 3.367 ± 0.501
3.902SerLys: 3.902 ± 0.467
4.208SerLeu: 4.208 ± 0.538
2.831SerMet: 2.831 ± 0.547
2.449SerAsn: 2.449 ± 0.352
2.602SerPro: 2.602 ± 0.412
2.449SerGln: 2.449 ± 0.451
2.984SerArg: 2.984 ± 0.417
3.29SerSer: 3.29 ± 0.526
3.596SerThr: 3.596 ± 0.625
3.52SerVal: 3.52 ± 0.564
0.689SerTrp: 0.689 ± 0.236
1.454SerTyr: 1.454 ± 0.313
0.0SerXaa: 0.0 ± 0.0
Thr
5.356ThrAla: 5.356 ± 0.692
0.306ThrCys: 0.306 ± 0.16
3.673ThrAsp: 3.673 ± 0.595
3.826ThrGlu: 3.826 ± 0.579
1.913ThrPhe: 1.913 ± 0.509
5.739ThrGly: 5.739 ± 0.721
0.383ThrHis: 0.383 ± 0.189
3.979ThrIle: 3.979 ± 0.508
3.137ThrLys: 3.137 ± 0.666
4.208ThrLeu: 4.208 ± 0.62
1.148ThrMet: 1.148 ± 0.337
1.836ThrAsn: 1.836 ± 0.306
3.902ThrPro: 3.902 ± 0.716
2.525ThrGln: 2.525 ± 0.443
3.902ThrArg: 3.902 ± 0.548
2.984ThrSer: 2.984 ± 0.456
3.137ThrThr: 3.137 ± 0.442
5.127ThrVal: 5.127 ± 0.74
1.071ThrTrp: 1.071 ± 0.363
1.989ThrTyr: 1.989 ± 0.319
0.0ThrXaa: 0.0 ± 0.0
Val
6.733ValAla: 6.733 ± 0.806
0.765ValCys: 0.765 ± 0.249
5.662ValAsp: 5.662 ± 0.613
3.749ValGlu: 3.749 ± 0.497
2.755ValPhe: 2.755 ± 0.609
5.05ValGly: 5.05 ± 0.606
1.301ValHis: 1.301 ± 0.395
3.061ValIle: 3.061 ± 0.453
3.29ValLys: 3.29 ± 0.715
4.668ValLeu: 4.668 ± 0.573
1.836ValMet: 1.836 ± 0.304
2.984ValAsn: 2.984 ± 0.561
1.989ValPro: 1.989 ± 0.454
2.219ValGln: 2.219 ± 0.336
3.443ValArg: 3.443 ± 0.527
4.974ValSer: 4.974 ± 0.585
4.821ValThr: 4.821 ± 0.521
4.668ValVal: 4.668 ± 0.635
1.224ValTrp: 1.224 ± 0.365
2.296ValTyr: 2.296 ± 0.445
0.0ValXaa: 0.0 ± 0.0
Trp
1.301TrpAla: 1.301 ± 0.322
0.153TrpCys: 0.153 ± 0.103
1.301TrpAsp: 1.301 ± 0.331
0.765TrpGlu: 0.765 ± 0.257
0.689TrpPhe: 0.689 ± 0.256
1.301TrpGly: 1.301 ± 0.301
0.077TrpHis: 0.077 ± 0.079
0.918TrpIle: 0.918 ± 0.306
0.842TrpLys: 0.842 ± 0.265
1.76TrpLeu: 1.76 ± 0.318
0.612TrpMet: 0.612 ± 0.194
0.689TrpAsn: 0.689 ± 0.235
0.689TrpPro: 0.689 ± 0.278
0.842TrpGln: 0.842 ± 0.226
1.377TrpArg: 1.377 ± 0.369
0.918TrpSer: 0.918 ± 0.265
0.842TrpThr: 0.842 ± 0.287
1.224TrpVal: 1.224 ± 0.263
0.459TrpTrp: 0.459 ± 0.178
0.536TrpTyr: 0.536 ± 0.174
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.755TyrAla: 2.755 ± 0.406
0.077TyrCys: 0.077 ± 0.085
2.219TyrAsp: 2.219 ± 0.395
2.066TyrGlu: 2.066 ± 0.437
1.454TyrPhe: 1.454 ± 0.355
2.678TyrGly: 2.678 ± 0.438
0.918TyrHis: 0.918 ± 0.272
1.148TyrIle: 1.148 ± 0.353
1.377TyrLys: 1.377 ± 0.408
1.76TyrLeu: 1.76 ± 0.323
0.765TyrMet: 0.765 ± 0.215
1.683TyrAsn: 1.683 ± 0.366
1.377TyrPro: 1.377 ± 0.381
0.995TyrGln: 0.995 ± 0.237
1.53TyrArg: 1.53 ± 0.384
2.066TyrSer: 2.066 ± 0.479
1.836TyrThr: 1.836 ± 0.376
2.984TyrVal: 2.984 ± 0.555
0.536TyrTrp: 0.536 ± 0.247
1.071TyrTyr: 1.071 ± 0.402
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 65 proteins (13070 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski