Microbacterium sp. RU33B

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3173 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1R3X9Y6|A0A1R3X9Y6_9MICO Glycerophosphoryl diester phosphodiesterase OS=Microbacterium sp. RU33B OX=1907390 GN=SAMN05880545_2871 PE=4 SV=1
MM1 pKa = 7.64AAAQRR6 pKa = 11.84KK7 pKa = 5.94TNWFAIWVSVGVVVVLVLVGALVVWMNTAAKK38 pKa = 10.5DD39 pKa = 3.56PGTLPEE45 pKa = 4.65SGGIDD50 pKa = 3.43TTTGAIAVGEE60 pKa = 4.36GANEE64 pKa = 3.75LATYY68 pKa = 10.08IDD70 pKa = 5.05FMCPICGDD78 pKa = 3.96FEE80 pKa = 4.79ASYY83 pKa = 10.9GEE85 pKa = 4.18EE86 pKa = 3.74MLGLVEE92 pKa = 6.37DD93 pKa = 4.28GTITLNIHH101 pKa = 7.23PISILDD107 pKa = 3.89RR108 pKa = 11.84YY109 pKa = 9.77SQGTQYY115 pKa = 9.57STRR118 pKa = 11.84AANAMYY124 pKa = 9.98CVADD128 pKa = 3.9SDD130 pKa = 4.04PTATVAFMQTLFEE143 pKa = 4.27NQPAEE148 pKa = 3.99QSEE151 pKa = 4.39GLSDD155 pKa = 4.11QEE157 pKa = 5.52LIDD160 pKa = 3.82FAKK163 pKa = 10.66GVTDD167 pKa = 5.18ADD169 pKa = 3.79VASCITDD176 pKa = 3.4GTYY179 pKa = 10.89KK180 pKa = 10.87DD181 pKa = 4.69FVAAMTEE188 pKa = 3.98KK189 pKa = 10.44TPIQPGASGIGTPTIAVNGEE209 pKa = 4.17VISNSTIPSPGSLATLFEE227 pKa = 4.4

Molecular weight: 23.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1R3VU72|A0A1R3VU72_9MICO Signal transduction histidine kinase OS=Microbacterium sp. RU33B OX=1907390 GN=SAMN05880545_0363 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3173

0

3173

1050477

29

2911

331.1

35.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.859 ± 0.069

0.467 ± 0.009

6.434 ± 0.035

5.567 ± 0.035

3.16 ± 0.024

8.949 ± 0.041

1.908 ± 0.021

4.562 ± 0.028

1.788 ± 0.028

10.049 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.725 ± 0.019

1.779 ± 0.023

5.517 ± 0.034

2.621 ± 0.02

7.316 ± 0.051

5.641 ± 0.03

6.042 ± 0.049

9.123 ± 0.04

1.569 ± 0.019

1.924 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski