Amino acid dipepetide frequency for Microbacterium sp. RU33B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.29AlaAla: 21.29 ± 0.225	0.683AlaCys: 0.683 ± 0.028	8.733AlaAsp: 8.733 ± 0.095	8.182AlaGlu: 8.182 ± 0.107	4.061AlaPhe: 4.061 ± 0.074	12.29AlaGly: 12.29 ± 0.125	2.529AlaHis: 2.529 ± 0.052	5.854AlaIle: 5.854 ± 0.091	2.493AlaLys: 2.493 ± 0.058	14.727AlaLeu: 14.727 ± 0.153	2.618AlaMet: 2.618 ± 0.05	2.148AlaAsn: 2.148 ± 0.05	7.105AlaPro: 7.105 ± 0.102	3.876AlaGln: 3.876 ± 0.074	9.641AlaArg: 9.641 ± 0.135	7.973AlaSer: 7.973 ± 0.098	7.563AlaThr: 7.563 ± 0.092	12.253AlaVal: 12.253 ± 0.119	2.093AlaTrp: 2.093 ± 0.045	2.481AlaTyr: 2.481 ± 0.053	0.0AlaXaa: 0.0 ± 0.0
Cys	0.664CysAla: 0.664 ± 0.024	0.044CysCys: 0.044 ± 0.006	0.31CysAsp: 0.31 ± 0.018	0.214CysGlu: 0.214 ± 0.012	0.155CysPhe: 0.155 ± 0.013	0.512CysGly: 0.512 ± 0.024	0.114CysHis: 0.114 ± 0.011	0.171CysIle: 0.171 ± 0.013	0.061CysLys: 0.061 ± 0.007	0.408CysLeu: 0.408 ± 0.019	0.069CysMet: 0.069 ± 0.011	0.103CysAsn: 0.103 ± 0.009	0.278CysPro: 0.278 ± 0.02	0.114CysGln: 0.114 ± 0.012	0.292CysArg: 0.292 ± 0.02	0.283CysSer: 0.283 ± 0.016	0.307CysThr: 0.307 ± 0.02	0.41CysVal: 0.41 ± 0.021	0.066CysTrp: 0.066 ± 0.008	0.089CysTyr: 0.089 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	10.252AspAla: 10.252 ± 0.106	0.196AspCys: 0.196 ± 0.015	4.863AspAsp: 4.863 ± 0.078	4.329AspGlu: 4.329 ± 0.077	1.713AspPhe: 1.713 ± 0.032	6.305AspGly: 6.305 ± 0.089	1.124AspHis: 1.124 ± 0.034	2.571AspIle: 2.571 ± 0.055	0.905AspLys: 0.905 ± 0.033	6.324AspLeu: 6.324 ± 0.093	0.85AspMet: 0.85 ± 0.03	0.892AspAsn: 0.892 ± 0.03	4.351AspPro: 4.351 ± 0.069	1.456AspGln: 1.456 ± 0.044	4.497AspArg: 4.497 ± 0.084	2.599AspSer: 2.599 ± 0.052	2.991AspThr: 2.991 ± 0.054	5.954AspVal: 5.954 ± 0.084	0.947AspTrp: 0.947 ± 0.032	1.222AspTyr: 1.222 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	7.205GluAla: 7.205 ± 0.114	0.23GluCys: 0.23 ± 0.013	2.567GluAsp: 2.567 ± 0.057	3.105GluGlu: 3.105 ± 0.072	1.77GluPhe: 1.77 ± 0.043	4.192GluGly: 4.192 ± 0.063	1.266GluHis: 1.266 ± 0.04	2.776GluIle: 2.776 ± 0.054	1.347GluLys: 1.347 ± 0.046	6.377GluLeu: 6.377 ± 0.077	0.851GluMet: 0.851 ± 0.026	1.147GluAsn: 1.147 ± 0.034	3.054GluPro: 3.054 ± 0.061	1.913GluGln: 1.913 ± 0.047	5.163GluArg: 5.163 ± 0.076	2.96GluSer: 2.96 ± 0.06	3.145GluThr: 3.145 ± 0.061	4.473GluVal: 4.473 ± 0.078	0.982GluTrp: 0.982 ± 0.032	1.139GluTyr: 1.139 ± 0.031	0.0GluXaa: 0.0 ± 0.0
Phe	4.631PheAla: 4.631 ± 0.069	0.149PheCys: 0.149 ± 0.012	2.436PheAsp: 2.436 ± 0.061	1.714PheGlu: 1.714 ± 0.042	1.144PhePhe: 1.144 ± 0.039	3.376PheGly: 3.376 ± 0.057	0.547PheHis: 0.547 ± 0.022	1.172PheIle: 1.172 ± 0.039	0.415PheLys: 0.415 ± 0.02	2.864PheLeu: 2.864 ± 0.06	0.417PheMet: 0.417 ± 0.022	0.63PheAsn: 0.63 ± 0.028	1.44PhePro: 1.44 ± 0.041	0.738PheGln: 0.738 ± 0.031	1.879PheArg: 1.879 ± 0.042	1.711PheSer: 1.711 ± 0.042	2.187PheThr: 2.187 ± 0.05	3.019PheVal: 3.019 ± 0.054	0.51PheTrp: 0.51 ± 0.026	0.62PheTyr: 0.62 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	11.114GlyAla: 11.114 ± 0.138	0.535GlyCys: 0.535 ± 0.027	5.462GlyAsp: 5.462 ± 0.076	5.049GlyGlu: 5.049 ± 0.067	3.404GlyPhe: 3.404 ± 0.054	7.873GlyGly: 7.873 ± 0.112	1.683GlyHis: 1.683 ± 0.038	5.031GlyIle: 5.031 ± 0.071	1.912GlyLys: 1.912 ± 0.047	8.678GlyLeu: 8.678 ± 0.106	1.945GlyMet: 1.945 ± 0.045	1.534GlyAsn: 1.534 ± 0.04	3.935GlyPro: 3.935 ± 0.066	2.265GlyGln: 2.265 ± 0.059	6.294GlyArg: 6.294 ± 0.081	5.375GlySer: 5.375 ± 0.084	5.453GlyThr: 5.453 ± 0.088	8.13GlyVal: 8.13 ± 0.091	1.679GlyTrp: 1.679 ± 0.048	2.143GlyTyr: 2.143 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	2.427HisAla: 2.427 ± 0.057	0.091HisCys: 0.091 ± 0.009	1.309HisAsp: 1.309 ± 0.039	1.017HisGlu: 1.017 ± 0.032	0.554HisPhe: 0.554 ± 0.024	1.93HisGly: 1.93 ± 0.046	0.504HisHis: 0.504 ± 0.024	0.746HisIle: 0.746 ± 0.027	0.252HisLys: 0.252 ± 0.018	1.929HisLeu: 1.929 ± 0.056	0.296HisMet: 0.296 ± 0.017	0.331HisAsn: 0.331 ± 0.017	1.491HisPro: 1.491 ± 0.047	0.419HisGln: 0.419 ± 0.021	1.503HisArg: 1.503 ± 0.046	0.937HisSer: 0.937 ± 0.029	0.967HisThr: 0.967 ± 0.027	1.693HisVal: 1.693 ± 0.042	0.293HisTrp: 0.293 ± 0.015	0.388HisTyr: 0.388 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.426IleAla: 7.426 ± 0.092	0.197IleCys: 0.197 ± 0.014	3.626IleAsp: 3.626 ± 0.069	2.809IleGlu: 2.809 ± 0.052	1.143IlePhe: 1.143 ± 0.033	4.673IleGly: 4.673 ± 0.083	0.697IleHis: 0.697 ± 0.025	1.795IleIle: 1.795 ± 0.051	0.672IleLys: 0.672 ± 0.03	3.911IleLeu: 3.911 ± 0.071	0.608IleMet: 0.608 ± 0.03	0.77IleAsn: 0.77 ± 0.028	2.537IlePro: 2.537 ± 0.046	0.943IleGln: 0.943 ± 0.031	2.671IleArg: 2.671 ± 0.055	2.304IleSer: 2.304 ± 0.051	2.627IleThr: 2.627 ± 0.062	4.986IleVal: 4.986 ± 0.08	0.516IleTrp: 0.516 ± 0.025	0.703IleTyr: 0.703 ± 0.025	0.0IleXaa: 0.0 ± 0.0
Lys	2.249LysAla: 2.249 ± 0.066	0.055LysCys: 0.055 ± 0.007	1.045LysAsp: 1.045 ± 0.038	0.864LysGlu: 0.864 ± 0.032	0.417LysPhe: 0.417 ± 0.021	1.523LysGly: 1.523 ± 0.042	0.393LysHis: 0.393 ± 0.022	0.868LysIle: 0.868 ± 0.033	0.696LysLys: 0.696 ± 0.034	1.647LysLeu: 1.647 ± 0.05	0.32LysMet: 0.32 ± 0.02	0.426LysAsn: 0.426 ± 0.021	1.084LysPro: 1.084 ± 0.036	0.549LysGln: 0.549 ± 0.027	1.335LysArg: 1.335 ± 0.035	1.041LysSer: 1.041 ± 0.036	1.246LysThr: 1.246 ± 0.036	1.509LysVal: 1.509 ± 0.047	0.224LysTrp: 0.224 ± 0.015	0.393LysTyr: 0.393 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	14.752LeuAla: 14.752 ± 0.134	0.471LeuCys: 0.471 ± 0.021	6.753LeuAsp: 6.753 ± 0.097	5.329LeuGlu: 5.329 ± 0.072	3.002LeuPhe: 3.002 ± 0.059	8.959LeuGly: 8.959 ± 0.107	1.79LeuHis: 1.79 ± 0.044	4.206LeuIle: 4.206 ± 0.076	1.571LeuLys: 1.571 ± 0.046	10.046LeuLeu: 10.046 ± 0.144	1.514LeuMet: 1.514 ± 0.038	1.601LeuAsn: 1.601 ± 0.045	5.557LeuPro: 5.557 ± 0.078	2.486LeuGln: 2.486 ± 0.047	7.699LeuArg: 7.699 ± 0.111	5.89LeuSer: 5.89 ± 0.084	6.313LeuThr: 6.313 ± 0.086	9.554LeuVal: 9.554 ± 0.122	1.339LeuTrp: 1.339 ± 0.039	1.654LeuTyr: 1.654 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	1.979MetAla: 1.979 ± 0.054	0.098MetCys: 0.098 ± 0.009	0.789MetAsp: 0.789 ± 0.03	0.603MetGlu: 0.603 ± 0.025	0.474MetPhe: 0.474 ± 0.027	1.286MetGly: 1.286 ± 0.042	0.33MetHis: 0.33 ± 0.019	0.865MetIle: 0.865 ± 0.032	0.381MetLys: 0.381 ± 0.02	1.816MetLeu: 1.816 ± 0.036	0.322MetMet: 0.322 ± 0.022	0.429MetAsn: 0.429 ± 0.02	1.168MetPro: 1.168 ± 0.036	0.475MetGln: 0.475 ± 0.022	1.321MetArg: 1.321 ± 0.035	1.478MetSer: 1.478 ± 0.04	1.708MetThr: 1.708 ± 0.036	1.238MetVal: 1.238 ± 0.039	0.196MetTrp: 0.196 ± 0.015	0.288MetTyr: 0.288 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.332AsnAla: 2.332 ± 0.048	0.104AsnCys: 0.104 ± 0.011	1.096AsnAsp: 1.096 ± 0.038	0.895AsnGlu: 0.895 ± 0.027	0.559AsnPhe: 0.559 ± 0.025	1.733AsnGly: 1.733 ± 0.054	0.322AsnHis: 0.322 ± 0.015	0.773AsnIle: 0.773 ± 0.03	0.347AsnLys: 0.347 ± 0.02	1.699AsnLeu: 1.699 ± 0.045	0.284AsnMet: 0.284 ± 0.016	0.394AsnAsn: 0.394 ± 0.023	1.435AsnPro: 1.435 ± 0.042	0.485AsnGln: 0.485 ± 0.025	1.177AsnArg: 1.177 ± 0.035	0.835AsnSer: 0.835 ± 0.031	1.061AsnThr: 1.061 ± 0.046	1.556AsnVal: 1.556 ± 0.047	0.295AsnTrp: 0.295 ± 0.015	0.406AsnTyr: 0.406 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	7.56ProAla: 7.56 ± 0.115	0.177ProCys: 0.177 ± 0.011	4.159ProAsp: 4.159 ± 0.062	3.677ProGlu: 3.677 ± 0.072	1.816ProPhe: 1.816 ± 0.037	5.082ProGly: 5.082 ± 0.077	1.176ProHis: 1.176 ± 0.036	2.139ProIle: 2.139 ± 0.046	0.924ProLys: 0.924 ± 0.034	5.149ProLeu: 5.149 ± 0.063	0.822ProMet: 0.822 ± 0.029	0.922ProAsn: 0.922 ± 0.031	2.672ProPro: 2.672 ± 0.069	1.518ProGln: 1.518 ± 0.034	3.683ProArg: 3.683 ± 0.079	3.281ProSer: 3.281 ± 0.063	3.324ProThr: 3.324 ± 0.068	5.08ProVal: 5.08 ± 0.074	0.958ProTrp: 0.958 ± 0.03	1.053ProTyr: 1.053 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	3.289GlnAla: 3.289 ± 0.062	0.105GlnCys: 0.105 ± 0.011	1.258GlnAsp: 1.258 ± 0.036	1.218GlnGlu: 1.218 ± 0.033	0.855GlnPhe: 0.855 ± 0.03	1.959GlnGly: 1.959 ± 0.042	0.568GlnHis: 0.568 ± 0.028	1.293GlnIle: 1.293 ± 0.035	0.584GlnLys: 0.584 ± 0.026	2.934GlnLeu: 2.934 ± 0.056	0.45GlnMet: 0.45 ± 0.024	0.581GlnAsn: 0.581 ± 0.022	1.455GlnPro: 1.455 ± 0.046	0.988GlnGln: 0.988 ± 0.036	2.318GlnArg: 2.318 ± 0.053	1.542GlnSer: 1.542 ± 0.044	1.562GlnThr: 1.562 ± 0.041	2.19GlnVal: 2.19 ± 0.046	0.49GlnTrp: 0.49 ± 0.021	0.571GlnTyr: 0.571 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	9.41ArgAla: 9.41 ± 0.125	0.276ArgCys: 0.276 ± 0.018	4.555ArgAsp: 4.555 ± 0.085	4.187ArgGlu: 4.187 ± 0.071	2.371ArgPhe: 2.371 ± 0.044	5.645ArgGly: 5.645 ± 0.083	1.537ArgHis: 1.537 ± 0.036	3.776ArgIle: 3.776 ± 0.061	1.217ArgLys: 1.217 ± 0.037	7.449ArgLeu: 7.449 ± 0.109	1.673ArgMet: 1.673 ± 0.038	1.184ArgAsn: 1.184 ± 0.033	3.769ArgPro: 3.769 ± 0.078	1.83ArgGln: 1.83 ± 0.039	6.751ArgArg: 6.751 ± 0.109	4.147ArgSer: 4.147 ± 0.064	4.414ArgThr: 4.414 ± 0.059	6.33ArgVal: 6.33 ± 0.089	1.159ArgTrp: 1.159 ± 0.036	1.48ArgTyr: 1.48 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	7.466SerAla: 7.466 ± 0.099	0.236SerCys: 0.236 ± 0.017	3.345SerAsp: 3.345 ± 0.06	2.8SerGlu: 2.8 ± 0.052	1.95SerPhe: 1.95 ± 0.042	5.977SerGly: 5.977 ± 0.088	1.029SerHis: 1.029 ± 0.031	2.679SerIle: 2.679 ± 0.057	0.934SerLys: 0.934 ± 0.029	5.574SerLeu: 5.574 ± 0.073	1.16SerMet: 1.16 ± 0.036	1.024SerAsn: 1.024 ± 0.034	3.245SerPro: 3.245 ± 0.05	1.327SerGln: 1.327 ± 0.033	3.785SerArg: 3.785 ± 0.062	3.363SerSer: 3.363 ± 0.067	3.585SerThr: 3.585 ± 0.065	4.865SerVal: 4.865 ± 0.085	0.947SerTrp: 0.947 ± 0.029	1.119SerTyr: 1.119 ± 0.039	0.0SerXaa: 0.0 ± 0.0
Thr	8.053ThrAla: 8.053 ± 0.13	0.302ThrCys: 0.302 ± 0.018	3.755ThrAsp: 3.755 ± 0.066	2.851ThrGlu: 2.851 ± 0.057	1.91ThrPhe: 1.91 ± 0.047	5.734ThrGly: 5.734 ± 0.08	1.16ThrHis: 1.16 ± 0.037	3.011ThrIle: 3.011 ± 0.063	1.092ThrLys: 1.092 ± 0.036	5.929ThrLeu: 5.929 ± 0.078	0.963ThrMet: 0.963 ± 0.03	1.112ThrAsn: 1.112 ± 0.038	3.991ThrPro: 3.991 ± 0.062	1.492ThrGln: 1.492 ± 0.041	3.756ThrArg: 3.756 ± 0.064	3.484ThrSer: 3.484 ± 0.074	3.982ThrThr: 3.982 ± 0.086	5.711ThrVal: 5.711 ± 0.102	0.941ThrTrp: 0.941 ± 0.033	1.195ThrTyr: 1.195 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	12.355ValAla: 12.355 ± 0.131	0.515ValCys: 0.515 ± 0.023	6.058ValAsp: 6.058 ± 0.081	5.053ValGlu: 5.053 ± 0.077	2.992ValPhe: 2.992 ± 0.057	7.391ValGly: 7.391 ± 0.1	1.709ValHis: 1.709 ± 0.05	4.301ValIle: 4.301 ± 0.064	1.466ValLys: 1.466 ± 0.043	9.262ValLeu: 9.262 ± 0.115	1.432ValMet: 1.432 ± 0.04	1.689ValAsn: 1.689 ± 0.039	4.844ValPro: 4.844 ± 0.075	2.289ValGln: 2.289 ± 0.045	6.543ValArg: 6.543 ± 0.086	5.144ValSer: 5.144 ± 0.08	5.769ValThr: 5.769 ± 0.094	9.545ValVal: 9.545 ± 0.134	1.337ValTrp: 1.337 ± 0.04	1.535ValTyr: 1.535 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.666TrpAla: 1.666 ± 0.035	0.092TrpCys: 0.092 ± 0.008	0.888TrpAsp: 0.888 ± 0.032	0.66TrpGlu: 0.66 ± 0.026	0.593TrpPhe: 0.593 ± 0.027	1.203TrpGly: 1.203 ± 0.043	0.327TrpHis: 0.327 ± 0.017	0.882TrpIle: 0.882 ± 0.032	0.287TrpLys: 0.287 ± 0.016	1.725TrpLeu: 1.725 ± 0.038	0.392TrpMet: 0.392 ± 0.021	0.44TrpAsn: 0.44 ± 0.023	0.728TrpPro: 0.728 ± 0.028	0.541TrpGln: 0.541 ± 0.025	1.297TrpArg: 1.297 ± 0.037	1.004TrpSer: 1.004 ± 0.037	1.047TrpThr: 1.047 ± 0.032	1.227TrpVal: 1.227 ± 0.038	0.383TrpTrp: 0.383 ± 0.021	0.312TrpTyr: 0.312 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.472TyrAla: 2.472 ± 0.059	0.11TyrCys: 0.11 ± 0.009	1.332TyrAsp: 1.332 ± 0.035	1.111TyrGlu: 1.111 ± 0.032	0.715TyrPhe: 0.715 ± 0.023	1.852TyrGly: 1.852 ± 0.046	0.269TyrHis: 0.269 ± 0.016	0.707TyrIle: 0.707 ± 0.027	0.327TyrLys: 0.327 ± 0.019	2.038TyrLeu: 2.038 ± 0.043	0.259TyrMet: 0.259 ± 0.014	0.43TyrAsn: 0.43 ± 0.018	1.035TyrPro: 1.035 ± 0.032	0.506TyrGln: 0.506 ± 0.022	1.555TyrArg: 1.555 ± 0.043	1.061TyrSer: 1.061 ± 0.036	1.17TyrThr: 1.17 ± 0.043	1.506TyrVal: 1.506 ± 0.037	0.338TyrTrp: 0.338 ± 0.018	0.446TyrTyr: 0.446 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3173 proteins (1050477 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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