Achromobacter phage vB_AxyP_19-32_Axy11
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A514CUD2|A0A514CUD2_9CAUD Uncharacterized protein OS=Achromobacter phage vB_AxyP_19-32_Axy11 OX=2591042 GN=Axy11_007 PE=4 SV=1
MM1 pKa = 7.2 YY2 pKa = 10.48 SWLDD6 pKa = 3.62 KK7 pKa = 10.76 IIEE10 pKa = 4.12 EE11 pKa = 3.93 QAAYY15 pKa = 11.03 YY16 pKa = 11.16 GMTPEE21 pKa = 4.12 KK22 pKa = 10.22 WEE24 pKa = 3.99 QLKK27 pKa = 10.01 EE28 pKa = 3.61 AAIQYY33 pKa = 9.58 EE34 pKa = 4.39 EE35 pKa = 4.56 EE36 pKa = 4.23 NPHH39 pKa = 5.02 VV40 pKa = 3.79
Molecular weight: 4.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.131
IPC2_protein 4.495
IPC_protein 4.253
Toseland 4.139
ProMoST 4.342
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.012
Rodwell 4.113
Grimsley 4.062
Solomon 4.164
Lehninger 4.113
Nozaki 4.304
DTASelect 4.317
Thurlkill 4.139
EMBOSS 4.037
Sillero 4.355
Patrickios 1.952
IPC_peptide 4.177
IPC2_peptide 4.355
IPC2.peptide.svr19 4.28
Protein with the highest isoelectric point:
>tr|A0A514CUA3|A0A514CUA3_9CAUD Uncharacterized protein OS=Achromobacter phage vB_AxyP_19-32_Axy11 OX=2591042 GN=Axy11_079 PE=4 SV=1
MM1 pKa = 6.12 VHH3 pKa = 6.67 RR4 pKa = 11.84 RR5 pKa = 11.84 VKK7 pKa = 10.46 KK8 pKa = 10.06 KK9 pKa = 10.22 IPIPVEE15 pKa = 4.46 PIPEE19 pKa = 3.77 WRR21 pKa = 11.84 KK22 pKa = 9.76 KK23 pKa = 10.06 LKK25 pKa = 10.66 SYY27 pKa = 7.92 TAQIGSVGAVLYY39 pKa = 10.55 GVFEE43 pKa = 4.23 VLQQGLFAVPRR54 pKa = 11.84 HH55 pKa = 5.66 LLEE58 pKa = 4.72 KK59 pKa = 10.12 IPYY62 pKa = 8.05 GSKK65 pKa = 9.65 IALSIWASVMLVKK78 pKa = 10.71 LFRR81 pKa = 11.84 LKK83 pKa = 10.62 RR84 pKa = 11.84 KK85 pKa = 9.31 EE86 pKa = 3.85 KK87 pKa = 10.69
Molecular weight: 10.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.244
IPC2_protein 9.926
IPC_protein 10.262
Toseland 10.877
ProMoST 10.452
Dawson 10.95
Bjellqvist 10.57
Wikipedia 11.082
Rodwell 11.506
Grimsley 10.979
Solomon 11.008
Lehninger 10.994
Nozaki 10.833
DTASelect 10.57
Thurlkill 10.847
EMBOSS 11.257
Sillero 10.862
Patrickios 11.242
IPC_peptide 11.023
IPC2_peptide 9.077
IPC2.peptide.svr19 8.62
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
81
0
81
22662
40
3437
279.8
31.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.289 ± 0.701
0.728 ± 0.134
5.829 ± 0.125
6.456 ± 0.243
3.548 ± 0.165
7.025 ± 0.221
1.972 ± 0.205
5.366 ± 0.307
6.165 ± 0.242
8.23 ± 0.282
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.292 ± 0.119
4.911 ± 0.209
4.598 ± 0.217
4.713 ± 0.232
4.911 ± 0.194
5.591 ± 0.185
5.759 ± 0.229
6.862 ± 0.234
1.461 ± 0.195
3.296 ± 0.238
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here