Aequorivita sublithincola (strain DSM 14238 / LMG 21431 / ACAM 643 / 9-3)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Aequorivita; Aequorivita sublithincola

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3134 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I3Z094|I3Z094_AEQSU 4-hydroxy-tetrahydrodipicolinate synthase OS=Aequorivita sublithincola (strain DSM 14238 / LMG 21431 / ACAM 643 / 9-3) OX=746697 GN=dapA PE=3 SV=1
MM1 pKa = 7.63KK2 pKa = 10.37KK3 pKa = 8.4ITLLLVCLIGLSGMVAAQNVQSSSTVDD30 pKa = 3.3DD31 pKa = 4.04LLNRR35 pKa = 11.84LSQIGTNAGDD45 pKa = 3.91LSAYY49 pKa = 7.47FTKK52 pKa = 10.52QEE54 pKa = 3.81QQTLHH59 pKa = 6.65NYY61 pKa = 9.55FNLNKK66 pKa = 10.0NAPTSATVKK75 pKa = 9.8YY76 pKa = 9.56AKK78 pKa = 9.9NVNGSGFGDD87 pKa = 5.08FILTGNTQAIQLNYY101 pKa = 9.95KK102 pKa = 9.09VASDD106 pKa = 3.81VEE108 pKa = 4.77SNRR111 pKa = 11.84GVMAIIAYY119 pKa = 7.22TDD121 pKa = 3.24RR122 pKa = 11.84PTFEE126 pKa = 3.87GAYY129 pKa = 9.5TGTLVNEE136 pKa = 4.72DD137 pKa = 3.74FSGGPGAGSILACGPVMSSGGDD159 pKa = 3.21GCFAAGEE166 pKa = 4.36LEE168 pKa = 4.62DD169 pKa = 4.75GFSITASSGGDD180 pKa = 3.34TIYY183 pKa = 10.45IGAGAIGNTSTLVGANTFADD203 pKa = 3.72TTVLNFSPDD212 pKa = 2.82GAYY215 pKa = 10.31AVGMDD220 pKa = 4.51LFVDD224 pKa = 3.95SVGNADD230 pKa = 3.1IRR232 pKa = 11.84VYY234 pKa = 11.73DD235 pKa = 3.5MGGTLMDD242 pKa = 4.11TFTVSNTANTEE253 pKa = 3.83NFIGLISDD261 pKa = 3.76DD262 pKa = 4.83AIGKK266 pKa = 9.26IEE268 pKa = 4.01IQAEE272 pKa = 3.9ADD274 pKa = 3.22AGEE277 pKa = 4.35LFGNLAFGTDD287 pKa = 4.18PIGGGGSGPAVCFGANNTTSSIITFDD313 pKa = 3.86PADD316 pKa = 3.76PAAFTTLGTSPAPVFEE332 pKa = 4.37NAGAVDD338 pKa = 4.28PNDD341 pKa = 3.35DD342 pKa = 3.29TTAYY346 pKa = 10.56VLDD349 pKa = 4.18SGGLFYY355 pKa = 11.1SVDD358 pKa = 3.95LTTGVYY364 pKa = 9.75TNLGTILAPGGNQWSGAEE382 pKa = 4.15FDD384 pKa = 5.19PISGTLYY391 pKa = 10.43AISVNGALTATTLSTIDD408 pKa = 3.6IGALTATTIGLTGMAGGISLMIDD431 pKa = 3.26ANGDD435 pKa = 3.79GYY437 pKa = 11.62SHH439 pKa = 8.09DD440 pKa = 3.61IADD443 pKa = 3.83DD444 pKa = 3.26NFYY447 pKa = 11.22YY448 pKa = 10.81VDD450 pKa = 4.33LASGTASPIGPLGFDD465 pKa = 3.53ANFGQGGTWIDD476 pKa = 3.56GDD478 pKa = 3.92PGFVYY483 pKa = 10.67LSAFDD488 pKa = 4.01SGAFQSQWRR497 pKa = 11.84RR498 pKa = 11.84VDD500 pKa = 3.39VLTGSSTVIGLFNGGADD517 pKa = 3.52QVGWSSAKK525 pKa = 10.14GSLAVGIAEE534 pKa = 3.98NALEE538 pKa = 4.39GFSYY542 pKa = 10.95APNPTSGVLSLKK554 pKa = 10.45SINNIDD560 pKa = 3.28TVAIYY565 pKa = 11.39NMLGQNVMSSKK576 pKa = 10.32IGATTSDD583 pKa = 4.14LDD585 pKa = 3.55ISSLKK590 pKa = 9.07TGTYY594 pKa = 9.86IMQVTVAGQTAAFRR608 pKa = 11.84VLKK611 pKa = 10.69NN612 pKa = 3.33

Molecular weight: 62.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3YV35|I3YV35_AEQSU ATP synthase subunit beta OS=Aequorivita sublithincola (strain DSM 14238 / LMG 21431 / ACAM 643 / 9-3) OX=746697 GN=atpD PE=3 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.32KK6 pKa = 9.59RR7 pKa = 11.84KK8 pKa = 7.05RR9 pKa = 11.84HH10 pKa = 5.11KK11 pKa = 10.59VATHH15 pKa = 5.21KK16 pKa = 10.3RR17 pKa = 11.84KK18 pKa = 9.62KK19 pKa = 9.04RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84ANRR25 pKa = 11.84HH26 pKa = 4.77KK27 pKa = 10.58KK28 pKa = 9.8KK29 pKa = 10.51KK30 pKa = 10.04

Molecular weight: 3.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3134

0

3134

1040270

29

4288

331.9

37.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.454 ± 0.042

0.724 ± 0.013

5.518 ± 0.042

6.905 ± 0.048

5.333 ± 0.04

6.522 ± 0.059

1.6 ± 0.021

7.896 ± 0.038

7.802 ± 0.062

9.222 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.254 ± 0.024

6.223 ± 0.047

3.455 ± 0.027

3.277 ± 0.025

3.35 ± 0.034

6.537 ± 0.035

5.791 ± 0.049

6.134 ± 0.036

1.012 ± 0.013

3.991 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski