Sapporo virus (isolate GI/Human/Germany/pJG-Sap01) (Hu/Dresden/pJG-Sap01/DE)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Picornavirales; Caliciviridae; Sapovirus; Sapporo virus; Sapovirus isolates

Average proteome isoelectric point is 7.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q672I1-2|POLG-2_SVSAP Isoform of Q672I1 Isoform Subgenomic capsid protein of Genome polyprotein OS=Sapporo virus (isolate GI/Human/Germany/pJG-Sap01) OX=291175 PE=4 SV=1
MM1 pKa = 7.68EE2 pKa = 5.79GNGSNPEE9 pKa = 4.03PKK11 pKa = 9.91QSNNPMVVDD20 pKa = 4.25PPGTTGPTTSHH31 pKa = 5.31VVVANPEE38 pKa = 4.18QPNGAAQRR46 pKa = 11.84LEE48 pKa = 4.03LAVATGAIQSNVPEE62 pKa = 4.99AIRR65 pKa = 11.84NCFAVFRR72 pKa = 11.84TFAWNDD78 pKa = 3.16RR79 pKa = 11.84MPTGTFLGSISLHH92 pKa = 6.21PNINPYY98 pKa = 8.33TAHH101 pKa = 7.35LSGMWAGWGGSFEE114 pKa = 4.2VRR116 pKa = 11.84LSISGSGVFAGRR128 pKa = 11.84IIASVIPPGVDD139 pKa = 2.98PSSIRR144 pKa = 11.84DD145 pKa = 3.72PGVLPHH151 pKa = 6.89AFVDD155 pKa = 3.85ARR157 pKa = 11.84ITEE160 pKa = 4.41PVSFMIPDD168 pKa = 3.52VRR170 pKa = 11.84AVDD173 pKa = 3.6YY174 pKa = 11.25HH175 pKa = 8.42RR176 pKa = 11.84MDD178 pKa = 3.7GAEE181 pKa = 4.27PTCSLGFWVYY191 pKa = 10.8QPLLNPFSTTAVSTCWVSVEE211 pKa = 4.43TKK213 pKa = 10.3PGGDD217 pKa = 3.57FDD219 pKa = 4.81FCLLRR224 pKa = 11.84PPGQQMEE231 pKa = 4.51NGVSPEE237 pKa = 3.65GLLPRR242 pKa = 11.84RR243 pKa = 11.84LGYY246 pKa = 10.5SRR248 pKa = 11.84GNRR251 pKa = 11.84VGGLVVGMVLVAEE264 pKa = 4.88HH265 pKa = 6.14KK266 pKa = 10.11QVNRR270 pKa = 11.84HH271 pKa = 5.13FNSNSVTFGWSTAPVNPMAAEE292 pKa = 3.8IVTNQAHH299 pKa = 5.38STSRR303 pKa = 11.84HH304 pKa = 3.86AWLSIGAQNKK314 pKa = 9.37GPLFPGIPNHH324 pKa = 6.28FPDD327 pKa = 4.05SCASTIVGAMDD338 pKa = 3.72TSLGGRR344 pKa = 11.84PSTGVCGPAISFQNNGDD361 pKa = 3.5VYY363 pKa = 11.26EE364 pKa = 4.6NDD366 pKa = 3.7TPSVMFATYY375 pKa = 10.72DD376 pKa = 3.63PLTSGTGVALTNSINPASLALVRR399 pKa = 11.84ISNNDD404 pKa = 2.83FDD406 pKa = 4.46TSGFANDD413 pKa = 3.76KK414 pKa = 10.55NVVVQMSWEE423 pKa = 4.37MYY425 pKa = 9.52TGTNQIRR432 pKa = 11.84GQVTPMSGTNYY443 pKa = 9.37TFTSTGANTLVLWQEE458 pKa = 3.84RR459 pKa = 11.84MLSYY463 pKa = 10.78DD464 pKa = 3.05GHH466 pKa = 5.24QAILYY471 pKa = 9.11SSQLEE476 pKa = 3.96RR477 pKa = 11.84TAEE480 pKa = 3.91YY481 pKa = 9.83FQNDD485 pKa = 3.16IVNIPEE491 pKa = 3.5NSMAVFNVEE500 pKa = 3.98TNSASFQIGIRR511 pKa = 11.84PDD513 pKa = 3.24GYY515 pKa = 9.73MVTGGSIGINVPLEE529 pKa = 3.92PEE531 pKa = 3.84TRR533 pKa = 11.84FQYY536 pKa = 11.03VGILPLSAALSGPSGNMGRR555 pKa = 11.84ARR557 pKa = 11.84RR558 pKa = 11.84VFQQ561 pKa = 3.97

Molecular weight: 60.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q672I1|POLG_SVSAP Genome polyprotein OS=Sapporo virus (isolate GI/Human/Germany/pJG-Sap01) OX=291175 PE=3 SV=1
MM1 pKa = 7.85APTQSQSRR9 pKa = 11.84ATTRR13 pKa = 11.84WSLTRR18 pKa = 11.84LAQQVRR24 pKa = 11.84PHH26 pKa = 6.47PTLLLLIRR34 pKa = 11.84SNPMGPHH41 pKa = 6.1SAWSWLLPLVQSNPMSLRR59 pKa = 11.84QYY61 pKa = 9.5ATALQSFVLLLGTTGCPRR79 pKa = 11.84EE80 pKa = 4.46LFLDD84 pKa = 4.31LYY86 pKa = 11.01RR87 pKa = 11.84FIPTLTRR94 pKa = 11.84TLLTSLGCGPGGAVVLRR111 pKa = 11.84SGYY114 pKa = 9.93RR115 pKa = 11.84SLVLACSLGVSLLLSYY131 pKa = 10.59HH132 pKa = 6.22QGLIPRR138 pKa = 11.84PSGTQACCLTLSLMLASLSQFLSS161 pKa = 3.48

Molecular weight: 17.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

1

4

3167

161

2280

791.8

86.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.241 ± 0.485

1.326 ± 0.306

4.168 ± 0.662

4.484 ± 0.759

4.2 ± 0.45

8.178 ± 0.585

2.4 ± 0.188

4.926 ± 0.55

2.873 ± 0.861

7.831 ± 2.349

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.716 ± 0.238

4.61 ± 0.877

6.599 ± 0.47

4.231 ± 0.729

5.526 ± 0.378

6.978 ± 1.492

8.304 ± 0.395

7.925 ± 0.868

1.674 ± 0.044

2.81 ± 0.455
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski