Mycobacterium phage NaSiaTalie

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A109QIR2|A0A109QIR2_9CAUD DNA primase OS=Mycobacterium phage NaSiaTalie OX=1777062 GN=59 PE=4 SV=1
MM1 pKa = 7.98DD2 pKa = 5.86PEE4 pKa = 4.18QLTLFDD10 pKa = 5.41LEE12 pKa = 5.43DD13 pKa = 3.11IHH15 pKa = 9.21DD16 pKa = 4.13YY17 pKa = 10.22VHH19 pKa = 6.94EE20 pKa = 4.98GEE22 pKa = 5.05DD23 pKa = 4.38DD24 pKa = 3.67EE25 pKa = 5.33

Molecular weight: 3.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Y0ABN5|A0A0Y0ABN5_9CAUD Major tail protein OS=Mycobacterium phage NaSiaTalie OX=1777062 GN=23 PE=4 SV=1
MM1 pKa = 7.74SSTKK5 pKa = 10.68LPFLHH10 pKa = 6.77KK11 pKa = 9.87NARR14 pKa = 11.84SRR16 pKa = 11.84RR17 pKa = 11.84ITSKK21 pKa = 9.47EE22 pKa = 3.65VRR24 pKa = 11.84EE25 pKa = 4.19VFADD29 pKa = 3.65EE30 pKa = 4.18VTRR33 pKa = 11.84GLDD36 pKa = 3.0RR37 pKa = 11.84RR38 pKa = 11.84LDD40 pKa = 3.49RR41 pKa = 11.84EE42 pKa = 3.95EE43 pKa = 3.64YY44 pKa = 10.08LRR46 pKa = 11.84RR47 pKa = 11.84VMPP50 pKa = 4.78

Molecular weight: 6.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

98

0

98

16576

25

835

169.1

18.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.779 ± 0.403

0.893 ± 0.134

6.31 ± 0.226

7.089 ± 0.324

3.427 ± 0.157

7.849 ± 0.33

2.069 ± 0.173

4.838 ± 0.159

4.742 ± 0.193

8.205 ± 0.327

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.528 ± 0.161

3.306 ± 0.199

5.484 ± 0.245

3.565 ± 0.219

6.642 ± 0.372

5.194 ± 0.271

6.184 ± 0.234

7.101 ± 0.159

1.9 ± 0.141

2.896 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski