Amino acid dipepetide frequency for Mycobacterium phage NaSiaTalie

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.739AlaAla: 10.739 ± 1.028
0.483AlaCys: 0.483 ± 0.151
5.128AlaAsp: 5.128 ± 0.561
7.964AlaGlu: 7.964 ± 0.868
3.439AlaPhe: 3.439 ± 0.556
7.903AlaGly: 7.903 ± 0.802
1.87AlaHis: 1.87 ± 0.328
3.801AlaIle: 3.801 ± 0.472
4.706AlaLys: 4.706 ± 0.597
8.627AlaLeu: 8.627 ± 1.009
2.534AlaMet: 2.534 ± 0.405
2.896AlaAsn: 2.896 ± 0.389
4.525AlaPro: 4.525 ± 0.542
3.982AlaGln: 3.982 ± 0.555
6.998AlaArg: 6.998 ± 0.719
4.827AlaSer: 4.827 ± 0.453
5.43AlaThr: 5.43 ± 0.52
7.421AlaVal: 7.421 ± 0.63
1.75AlaTrp: 1.75 ± 0.351
2.715AlaTyr: 2.715 ± 0.43
0.0AlaXaa: 0.0 ± 0.0
Cys
0.543CysAla: 0.543 ± 0.176
0.0CysCys: 0.0 ± 0.0
0.664CysAsp: 0.664 ± 0.227
0.483CysGlu: 0.483 ± 0.14
0.302CysPhe: 0.302 ± 0.146
0.905CysGly: 0.905 ± 0.24
0.422CysHis: 0.422 ± 0.17
0.241CysIle: 0.241 ± 0.112
0.543CysLys: 0.543 ± 0.166
0.603CysLeu: 0.603 ± 0.218
0.302CysMet: 0.302 ± 0.162
0.362CysAsn: 0.362 ± 0.138
0.543CysPro: 0.543 ± 0.223
0.181CysGln: 0.181 ± 0.099
0.483CysArg: 0.483 ± 0.16
0.543CysSer: 0.543 ± 0.187
0.603CysThr: 0.603 ± 0.153
0.543CysVal: 0.543 ± 0.147
0.362CysTrp: 0.362 ± 0.144
0.302CysTyr: 0.302 ± 0.122
0.0CysXaa: 0.0 ± 0.0
Asp
6.576AspAla: 6.576 ± 0.706
0.664AspCys: 0.664 ± 0.194
3.499AspAsp: 3.499 ± 0.46
5.128AspGlu: 5.128 ± 0.685
3.017AspPhe: 3.017 ± 0.506
5.611AspGly: 5.611 ± 0.555
1.448AspHis: 1.448 ± 0.351
3.258AspIle: 3.258 ± 0.385
2.413AspLys: 2.413 ± 0.366
5.189AspLeu: 5.189 ± 0.648
1.81AspMet: 1.81 ± 0.271
1.629AspAsn: 1.629 ± 0.41
4.585AspPro: 4.585 ± 0.621
1.991AspGln: 1.991 ± 0.271
2.715AspArg: 2.715 ± 0.409
2.896AspSer: 2.896 ± 0.36
2.896AspThr: 2.896 ± 0.381
4.344AspVal: 4.344 ± 0.475
1.146AspTrp: 1.146 ± 0.27
2.293AspTyr: 2.293 ± 0.353
0.0AspXaa: 0.0 ± 0.0
Glu
7.662GluAla: 7.662 ± 0.646
0.362GluCys: 0.362 ± 0.197
5.309GluAsp: 5.309 ± 0.699
4.585GluGlu: 4.585 ± 0.653
2.896GluPhe: 2.896 ± 0.431
5.128GluGly: 5.128 ± 0.673
1.931GluHis: 1.931 ± 0.333
4.465GluIle: 4.465 ± 0.522
2.172GluLys: 2.172 ± 0.306
7.3GluLeu: 7.3 ± 0.88
2.051GluMet: 2.051 ± 0.313
1.931GluAsn: 1.931 ± 0.284
2.956GluPro: 2.956 ± 0.448
2.051GluGln: 2.051 ± 0.345
4.404GluArg: 4.404 ± 0.623
3.198GluSer: 3.198 ± 0.462
4.223GluThr: 4.223 ± 0.449
4.706GluVal: 4.706 ± 0.478
1.327GluTrp: 1.327 ± 0.31
2.232GluTyr: 2.232 ± 0.372
0.0GluXaa: 0.0 ± 0.0
Phe
3.379PheAla: 3.379 ± 0.362
0.362PheCys: 0.362 ± 0.149
2.413PheAsp: 2.413 ± 0.465
2.836PheGlu: 2.836 ± 0.368
0.784PhePhe: 0.784 ± 0.227
3.017PheGly: 3.017 ± 0.39
0.905PheHis: 0.905 ± 0.287
1.026PheIle: 1.026 ± 0.215
1.267PheLys: 1.267 ± 0.296
2.775PheLeu: 2.775 ± 0.472
0.784PheMet: 0.784 ± 0.221
1.931PheAsn: 1.931 ± 0.308
1.87PhePro: 1.87 ± 0.29
1.207PheGln: 1.207 ± 0.331
2.474PheArg: 2.474 ± 0.32
1.81PheSer: 1.81 ± 0.284
2.293PheThr: 2.293 ± 0.329
2.172PheVal: 2.172 ± 0.317
0.362PheTrp: 0.362 ± 0.139
0.603PheTyr: 0.603 ± 0.174
0.0PheXaa: 0.0 ± 0.0
Gly
6.094GlyAla: 6.094 ± 0.846
0.724GlyCys: 0.724 ± 0.209
5.611GlyAsp: 5.611 ± 0.611
5.128GlyGlu: 5.128 ± 0.54
3.499GlyPhe: 3.499 ± 0.433
7.36GlyGly: 7.36 ± 1.167
1.689GlyHis: 1.689 ± 0.359
4.042GlyIle: 4.042 ± 0.48
4.163GlyLys: 4.163 ± 0.498
5.732GlyLeu: 5.732 ± 0.765
1.931GlyMet: 1.931 ± 0.408
3.137GlyAsn: 3.137 ± 0.444
3.741GlyPro: 3.741 ± 0.459
3.258GlyGln: 3.258 ± 0.476
3.56GlyArg: 3.56 ± 0.418
3.861GlySer: 3.861 ± 0.505
5.068GlyThr: 5.068 ± 0.701
5.852GlyVal: 5.852 ± 0.655
1.388GlyTrp: 1.388 ± 0.298
2.655GlyTyr: 2.655 ± 0.357
0.0GlyXaa: 0.0 ± 0.0
His
1.629HisAla: 1.629 ± 0.35
0.362HisCys: 0.362 ± 0.137
1.75HisAsp: 1.75 ± 0.315
1.388HisGlu: 1.388 ± 0.302
0.543HisPhe: 0.543 ± 0.192
1.75HisGly: 1.75 ± 0.411
0.483HisHis: 0.483 ± 0.177
1.388HisIle: 1.388 ± 0.286
0.965HisLys: 0.965 ± 0.216
1.508HisLeu: 1.508 ± 0.259
0.543HisMet: 0.543 ± 0.178
0.362HisAsn: 0.362 ± 0.126
1.267HisPro: 1.267 ± 0.237
1.026HisGln: 1.026 ± 0.249
1.629HisArg: 1.629 ± 0.378
0.905HisSer: 0.905 ± 0.215
0.965HisThr: 0.965 ± 0.267
1.327HisVal: 1.327 ± 0.305
0.362HisTrp: 0.362 ± 0.158
0.543HisTyr: 0.543 ± 0.205
0.0HisXaa: 0.0 ± 0.0
Ile
5.551IleAla: 5.551 ± 0.576
0.483IleCys: 0.483 ± 0.131
3.499IleAsp: 3.499 ± 0.423
4.465IleGlu: 4.465 ± 0.49
1.086IlePhe: 1.086 ± 0.237
4.042IleGly: 4.042 ± 0.404
1.146IleHis: 1.146 ± 0.287
1.508IleIle: 1.508 ± 0.281
2.112IleLys: 2.112 ± 0.372
3.922IleLeu: 3.922 ± 0.391
0.543IleMet: 0.543 ± 0.194
1.569IleAsn: 1.569 ± 0.353
3.62IlePro: 3.62 ± 0.493
1.508IleGln: 1.508 ± 0.358
3.258IleArg: 3.258 ± 0.486
2.172IleSer: 2.172 ± 0.314
3.379IleThr: 3.379 ± 0.361
2.956IleVal: 2.956 ± 0.465
0.543IleTrp: 0.543 ± 0.167
1.026IleTyr: 1.026 ± 0.25
0.0IleXaa: 0.0 ± 0.0
Lys
5.37LysAla: 5.37 ± 0.624
0.422LysCys: 0.422 ± 0.177
2.474LysAsp: 2.474 ± 0.376
2.232LysGlu: 2.232 ± 0.421
1.026LysPhe: 1.026 ± 0.223
3.62LysGly: 3.62 ± 0.615
0.543LysHis: 0.543 ± 0.18
2.655LysIle: 2.655 ± 0.441
3.318LysLys: 3.318 ± 0.457
4.223LysLeu: 4.223 ± 0.503
0.965LysMet: 0.965 ± 0.252
1.388LysAsn: 1.388 ± 0.358
2.896LysPro: 2.896 ± 0.477
1.75LysGln: 1.75 ± 0.324
2.956LysArg: 2.956 ± 0.49
2.112LysSer: 2.112 ± 0.37
3.077LysThr: 3.077 ± 0.441
4.344LysVal: 4.344 ± 0.475
0.724LysTrp: 0.724 ± 0.218
1.327LysTyr: 1.327 ± 0.25
0.0LysXaa: 0.0 ± 0.0
Leu
9.412LeuAla: 9.412 ± 1.092
0.543LeuCys: 0.543 ± 0.194
4.827LeuAsp: 4.827 ± 0.607
5.611LeuGlu: 5.611 ± 0.691
2.293LeuPhe: 2.293 ± 0.26
5.611LeuGly: 5.611 ± 0.591
1.569LeuHis: 1.569 ± 0.33
4.284LeuIle: 4.284 ± 0.461
3.439LeuLys: 3.439 ± 0.481
6.335LeuLeu: 6.335 ± 0.641
2.715LeuMet: 2.715 ± 0.401
2.474LeuAsn: 2.474 ± 0.382
4.646LeuPro: 4.646 ± 0.462
2.534LeuGln: 2.534 ± 0.484
6.214LeuArg: 6.214 ± 0.75
4.646LeuSer: 4.646 ± 0.738
6.395LeuThr: 6.395 ± 0.774
4.766LeuVal: 4.766 ± 0.654
1.448LeuTrp: 1.448 ± 0.283
2.293LeuTyr: 2.293 ± 0.375
0.0LeuXaa: 0.0 ± 0.0
Met
2.836MetAla: 2.836 ± 0.39
0.06MetCys: 0.06 ± 0.055
0.905MetAsp: 0.905 ± 0.236
1.267MetGlu: 1.267 ± 0.248
0.422MetPhe: 0.422 ± 0.143
1.569MetGly: 1.569 ± 0.301
0.543MetHis: 0.543 ± 0.152
1.81MetIle: 1.81 ± 0.307
2.051MetLys: 2.051 ± 0.347
1.689MetLeu: 1.689 ± 0.309
0.483MetMet: 0.483 ± 0.143
0.724MetAsn: 0.724 ± 0.209
1.448MetPro: 1.448 ± 0.332
0.845MetGln: 0.845 ± 0.202
1.508MetArg: 1.508 ± 0.268
2.232MetSer: 2.232 ± 0.351
2.534MetThr: 2.534 ± 0.38
1.207MetVal: 1.207 ± 0.253
0.362MetTrp: 0.362 ± 0.155
0.784MetTyr: 0.784 ± 0.225
0.0MetXaa: 0.0 ± 0.0
Asn
3.258AsnAla: 3.258 ± 0.469
0.543AsnCys: 0.543 ± 0.17
1.81AsnAsp: 1.81 ± 0.28
2.413AsnGlu: 2.413 ± 0.416
0.784AsnPhe: 0.784 ± 0.236
3.379AsnGly: 3.379 ± 0.679
0.845AsnHis: 0.845 ± 0.197
1.388AsnIle: 1.388 ± 0.283
1.508AsnLys: 1.508 ± 0.313
2.956AsnLeu: 2.956 ± 0.455
0.784AsnMet: 0.784 ± 0.215
0.965AsnAsn: 0.965 ± 0.288
2.353AsnPro: 2.353 ± 0.368
0.845AsnGln: 0.845 ± 0.198
2.232AsnArg: 2.232 ± 0.42
1.388AsnSer: 1.388 ± 0.257
1.267AsnThr: 1.267 ± 0.246
2.836AsnVal: 2.836 ± 0.497
0.724AsnTrp: 0.724 ± 0.219
0.784AsnTyr: 0.784 ± 0.199
0.0AsnXaa: 0.0 ± 0.0
Pro
5.913ProAla: 5.913 ± 0.599
0.422ProCys: 0.422 ± 0.175
4.042ProAsp: 4.042 ± 0.581
4.465ProGlu: 4.465 ± 0.537
2.232ProPhe: 2.232 ± 0.409
4.525ProGly: 4.525 ± 0.692
1.086ProHis: 1.086 ± 0.211
2.112ProIle: 2.112 ± 0.367
2.775ProLys: 2.775 ± 0.582
3.439ProLeu: 3.439 ± 0.422
1.267ProMet: 1.267 ± 0.272
1.991ProAsn: 1.991 ± 0.364
2.232ProPro: 2.232 ± 0.42
1.569ProGln: 1.569 ± 0.309
3.922ProArg: 3.922 ± 0.55
2.534ProSer: 2.534 ± 0.329
3.499ProThr: 3.499 ± 0.498
4.042ProVal: 4.042 ± 0.523
1.448ProTrp: 1.448 ± 0.307
1.327ProTyr: 1.327 ± 0.284
0.0ProXaa: 0.0 ± 0.0
Gln
3.198GlnAla: 3.198 ± 0.5
0.181GlnCys: 0.181 ± 0.117
1.508GlnAsp: 1.508 ± 0.3
1.689GlnGlu: 1.689 ± 0.304
1.026GlnPhe: 1.026 ± 0.256
2.534GlnGly: 2.534 ± 0.328
0.724GlnHis: 0.724 ± 0.183
2.413GlnIle: 2.413 ± 0.347
1.689GlnLys: 1.689 ± 0.366
3.62GlnLeu: 3.62 ± 0.579
1.026GlnMet: 1.026 ± 0.238
1.448GlnAsn: 1.448 ± 0.397
1.689GlnPro: 1.689 ± 0.315
2.172GlnGln: 2.172 ± 0.415
2.474GlnArg: 2.474 ± 0.421
1.87GlnSer: 1.87 ± 0.354
1.931GlnThr: 1.931 ± 0.312
2.715GlnVal: 2.715 ± 0.408
0.905GlnTrp: 0.905 ± 0.241
0.845GlnTyr: 0.845 ± 0.207
0.0GlnXaa: 0.0 ± 0.0
Arg
5.551ArgAla: 5.551 ± 0.611
0.965ArgCys: 0.965 ± 0.217
4.465ArgAsp: 4.465 ± 0.504
5.611ArgGlu: 5.611 ± 0.721
2.775ArgPhe: 2.775 ± 0.472
3.861ArgGly: 3.861 ± 0.509
1.388ArgHis: 1.388 ± 0.322
3.198ArgIle: 3.198 ± 0.448
3.499ArgLys: 3.499 ± 0.513
5.611ArgLeu: 5.611 ± 0.633
2.172ArgMet: 2.172 ± 0.409
2.172ArgAsn: 2.172 ± 0.395
2.715ArgPro: 2.715 ± 0.426
2.293ArgGln: 2.293 ± 0.362
6.275ArgArg: 6.275 ± 0.655
3.017ArgSer: 3.017 ± 0.421
3.198ArgThr: 3.198 ± 0.386
4.223ArgVal: 4.223 ± 0.507
1.327ArgTrp: 1.327 ± 0.299
2.112ArgTyr: 2.112 ± 0.356
0.0ArgXaa: 0.0 ± 0.0
Ser
3.922SerAla: 3.922 ± 0.509
0.543SerCys: 0.543 ± 0.143
3.198SerAsp: 3.198 ± 0.424
2.775SerGlu: 2.775 ± 0.465
2.232SerPhe: 2.232 ± 0.421
4.163SerGly: 4.163 ± 0.569
0.905SerHis: 0.905 ± 0.22
2.293SerIle: 2.293 ± 0.32
3.077SerLys: 3.077 ± 0.407
4.223SerLeu: 4.223 ± 0.567
1.388SerMet: 1.388 ± 0.258
1.569SerAsn: 1.569 ± 0.312
3.077SerPro: 3.077 ± 0.429
1.87SerGln: 1.87 ± 0.366
3.499SerArg: 3.499 ± 0.508
3.318SerSer: 3.318 ± 0.585
3.318SerThr: 3.318 ± 0.471
3.439SerVal: 3.439 ± 0.531
1.267SerTrp: 1.267 ± 0.271
1.87SerTyr: 1.87 ± 0.306
0.0SerXaa: 0.0 ± 0.0
Thr
5.551ThrAla: 5.551 ± 0.513
0.845ThrCys: 0.845 ± 0.349
3.861ThrAsp: 3.861 ± 0.516
3.922ThrGlu: 3.922 ± 0.549
2.051ThrPhe: 2.051 ± 0.306
5.189ThrGly: 5.189 ± 0.758
0.965ThrHis: 0.965 ± 0.262
2.413ThrIle: 2.413 ± 0.376
3.258ThrLys: 3.258 ± 0.468
5.309ThrLeu: 5.309 ± 0.755
1.388ThrMet: 1.388 ± 0.262
1.629ThrAsn: 1.629 ± 0.399
4.585ThrPro: 4.585 ± 0.669
2.112ThrGln: 2.112 ± 0.261
3.077ThrArg: 3.077 ± 0.373
2.775ThrSer: 2.775 ± 0.417
3.922ThrThr: 3.922 ± 0.57
5.852ThrVal: 5.852 ± 0.658
1.267ThrTrp: 1.267 ± 0.285
1.87ThrTyr: 1.87 ± 0.382
0.0ThrXaa: 0.0 ± 0.0
Val
6.817ValAla: 6.817 ± 0.772
0.483ValCys: 0.483 ± 0.153
5.068ValAsp: 5.068 ± 0.634
5.068ValGlu: 5.068 ± 0.573
2.293ValPhe: 2.293 ± 0.417
4.284ValGly: 4.284 ± 0.459
1.086ValHis: 1.086 ± 0.28
3.741ValIle: 3.741 ± 0.546
3.258ValLys: 3.258 ± 0.437
5.189ValLeu: 5.189 ± 0.643
1.569ValMet: 1.569 ± 0.398
3.017ValAsn: 3.017 ± 0.412
3.56ValPro: 3.56 ± 0.465
2.232ValGln: 2.232 ± 0.336
4.706ValArg: 4.706 ± 0.546
4.887ValSer: 4.887 ± 0.64
5.128ValThr: 5.128 ± 0.501
5.128ValVal: 5.128 ± 0.476
1.327ValTrp: 1.327 ± 0.326
2.172ValTyr: 2.172 ± 0.421
0.0ValXaa: 0.0 ± 0.0
Trp
1.267TrpAla: 1.267 ± 0.313
0.302TrpCys: 0.302 ± 0.149
1.026TrpAsp: 1.026 ± 0.222
1.327TrpGlu: 1.327 ± 0.25
0.483TrpPhe: 0.483 ± 0.173
1.448TrpGly: 1.448 ± 0.276
0.543TrpHis: 0.543 ± 0.211
1.026TrpIle: 1.026 ± 0.216
0.664TrpLys: 0.664 ± 0.195
1.388TrpLeu: 1.388 ± 0.288
0.543TrpMet: 0.543 ± 0.157
1.086TrpAsn: 1.086 ± 0.266
1.146TrpPro: 1.146 ± 0.313
0.965TrpGln: 0.965 ± 0.236
1.327TrpArg: 1.327 ± 0.233
1.207TrpSer: 1.207 ± 0.331
1.026TrpThr: 1.026 ± 0.278
1.207TrpVal: 1.207 ± 0.246
0.422TrpTrp: 0.422 ± 0.157
0.603TrpTyr: 0.603 ± 0.191
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.534TyrAla: 2.534 ± 0.413
0.181TyrCys: 0.181 ± 0.115
2.051TyrAsp: 2.051 ± 0.348
2.534TyrGlu: 2.534 ± 0.385
1.086TyrPhe: 1.086 ± 0.235
2.594TyrGly: 2.594 ± 0.383
0.603TyrHis: 0.603 ± 0.19
1.327TyrIle: 1.327 ± 0.252
0.543TyrLys: 0.543 ± 0.21
2.413TyrLeu: 2.413 ± 0.362
0.422TyrMet: 0.422 ± 0.181
0.784TyrAsn: 0.784 ± 0.228
1.388TyrPro: 1.388 ± 0.308
1.267TyrGln: 1.267 ± 0.283
2.715TyrArg: 2.715 ± 0.397
1.75TyrSer: 1.75 ± 0.332
1.689TyrThr: 1.689 ± 0.289
1.931TyrVal: 1.931 ± 0.406
0.543TyrTrp: 0.543 ± 0.18
0.603TyrTyr: 0.603 ± 0.193
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 98 proteins (16576 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski