Pasteurella phage AFS-2018a

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Bcepmuvirus; unclassified Bcepmuvirus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4PZI5|A0A2Z4PZI5_9CAUD Peptidase OS=Pasteurella phage AFS-2018a OX=2201988 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.14KK3 pKa = 10.37VKK5 pKa = 10.05LRR7 pKa = 11.84NGNDD11 pKa = 3.08AEE13 pKa = 4.33IVYY16 pKa = 10.51EE17 pKa = 4.05SDD19 pKa = 3.07FGKK22 pKa = 10.87LLVVEE27 pKa = 4.4KK28 pKa = 10.29TGDD31 pKa = 3.58EE32 pKa = 4.1LPAVHH37 pKa = 6.45WHH39 pKa = 5.81NADD42 pKa = 2.88GSFYY46 pKa = 10.93ADD48 pKa = 4.23CEE50 pKa = 4.37SSLDD54 pKa = 3.66IVV56 pKa = 3.85

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4PZG7|A0A2Z4PZG7_9CAUD Host-nuclease inhibitor protein OS=Pasteurella phage AFS-2018a OX=2201988 PE=4 SV=1
MM1 pKa = 7.46LKK3 pKa = 10.25ININDD8 pKa = 3.4QQVGIKK14 pKa = 9.44LQQIAIQLQHH24 pKa = 6.21PRR26 pKa = 11.84KK27 pKa = 9.68LYY29 pKa = 10.2GVLGEE34 pKa = 4.39TLKK37 pKa = 9.75KK38 pKa = 7.18THH40 pKa = 6.67KK41 pKa = 10.28EE42 pKa = 3.63RR43 pKa = 11.84FKK45 pKa = 11.34QEE47 pKa = 3.31VDD49 pKa = 2.99PDD51 pKa = 3.92GKK53 pKa = 10.28KK54 pKa = 8.94WRR56 pKa = 11.84ALSPVTLALKK66 pKa = 10.43AKK68 pKa = 9.83RR69 pKa = 11.84GKK71 pKa = 9.86SPKK74 pKa = 9.49ILRR77 pKa = 11.84QEE79 pKa = 4.81GYY81 pKa = 10.77LSDD84 pKa = 3.55KK85 pKa = 9.85TAYY88 pKa = 10.62NYY90 pKa = 10.57DD91 pKa = 3.64DD92 pKa = 3.59QGLEE96 pKa = 3.94FGSAAKK102 pKa = 10.09YY103 pKa = 10.59ARR105 pKa = 11.84LHH107 pKa = 5.39QFGGQAGRR115 pKa = 11.84GRR117 pKa = 11.84KK118 pKa = 7.1VTIPQRR124 pKa = 11.84KK125 pKa = 6.82WLGISKK131 pKa = 10.25NDD133 pKa = 3.79EE134 pKa = 4.07PLLLAKK140 pKa = 9.24ATSLLQRR147 pKa = 11.84QISKK151 pKa = 10.51IVGG154 pKa = 3.21

Molecular weight: 17.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

10143

56

782

230.5

25.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.42 ± 0.388

0.779 ± 0.129

5.373 ± 0.233

7.72 ± 0.454

3.618 ± 0.219

6.625 ± 0.314

1.923 ± 0.208

6.606 ± 0.257

7.808 ± 0.37

9.228 ± 0.363

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.169 ± 0.225

4.939 ± 0.316

3.184 ± 0.159

4.506 ± 0.226

4.742 ± 0.337

5.915 ± 0.29

5.649 ± 0.283

6.27 ± 0.218

1.43 ± 0.134

3.096 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski